pCMV-ELOC(human)-3×HA-CAG-ELOB(human)-6×His-Neo
$70.00
In stock
SKU
PL24217
-
100μg liquid plasmid(Instantly):$91
| name | pCMV-ELOC(human)-3×HA-CAG-ELOB(human)-6×His-Neo | another name | apCMV-ELOC(human)-3×HA-CAG-ELOB(human)-6×His-Neo |
| Insert Size (bp) | 354bp+288bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pCMV-MCS-3×FLAG-CAG-mCherry-Neo | 5′ sequencing primer | pcaggs-F |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CAGCATATGG GCATATGTTG CCAAACTCTA AACCAAATAC TCATTCTGAT GTTTTAAATG ATTTGCCCTC CCATATGTCC TTCCGAGTGA GAGACACAAA AAATTCCAAC ACACTATTGC AATGAAAATA AATTTCCTTT ATTAGCCAGA AGTCAGATGC TCAAGGGGCT TCATGATGTC CCCATAATTT TTGGCAGAGG GAAAAAGATC TTCAATGGTG ATGATGGTGA TGCTGCACAG CTTGTTCATT GGCACTGCTT CCCGAGTCCT GGGGCTTCAT CACATCGGGC AGCTCTGGCG GGCTGGAAAA CGGCTCGATG CACAGGGCCT CGAAGGTGTC ATCTGCCCGG AAGGCCAGCC CCACTGTGGC TGGGGCCTGT GGCCGTGCTG TTTGACTGGT GAAGCCACAC TCGCCCAGTG TCTTGCCATC ATCCAAGAGT TGGTCATCCT TGTACAGCCG CTGCTCGTCA GGAGGCCGCT TGAGGATGCC CTCGACGATG CGCTTCAGTT CGAACACCGT GCTGGACTCC TTGGCGTCCG TGAAGATGGT GGTCTTGTGG CGCCGGATCA TGAGAAACAC GTCCATGGTG GCCTCGAGGA ATTCTTTGCC AAAATGATGA GACAGCACAA TAACCAGCAC GTTGCCCAGG AGCTGTAGGA AAAAGAAGAA GGCATGAACA TGGTTAGCAG AGGCTCTAGA GCCGCCGGTC ACACGCCAGA AGCCGAACCC CGCCCTGCCC CGTCCCCCCC GAAGGCAGCC GTCCCCCCGC GGACAGCCCC GAGGCTGGAG AGGGAGAAGG GGACGGCGGC GCGGCGACGC ACGAAGGCCC TCCCCGCCCA TTTCCTTCCT GCCGGCGCCG CACCGCTTCG CCCCGCGCCC GCTAGAGGGG GTGCGGCGGC GCCTCCCAGA TTTCGGCTCC GCACAGATTT GGGACAAAGG AAGTCCCTGC GCCCTCTCGC ACGATTACCA TAAAAGGCAA TGGCTGCGGC TCGCCGCGCC TCGACAGCCG CCGGCGCTCC GGGGGCCGCC GCGCCCCTTC CCCCGAGCCC TCCCCGGCCC GAGGCGGCCC CGCCCCGCCC GGCACCCCCA CCTGCCGCCA CCCCCCGCCC GGCACGGCGA GCCCCGCGCC ACGCCCCGTA CGGAGCCCCG CACCCGAAGC CGGGCCGTGC TCAGCAACTC GGGGAGGGGG GTGCAGGGGG GGTTGCAGCC CGACCGACGC GCCCACACCC CCTGCTCACC CCCCCACGCA CACACCCCGC ACGCAGCCTT TGTTCCCCTC GCAGCCCCCC CCGCACCGCG GGGCACCGCC CCCGGCCGCG CTCCCCTCGC GCACACTGCG GAGCGCACAA AGCCCCGCGC CGCGCCCGCA GCGCTCACAG CCGCCGGGCA GCGCGGAGCC GCACGCGGCG CTCCCCACGC ACACACACAC GCACGCACCC CCCGAGCCGC TCCCCCCGCA CAAAGGGCCC TCCCGGAGCC CCTCAAGGCT TTCACGCAGC CACAGAAAAG AAACAAGCCG TCATTAAACC AAGCGCTAAT TACAGCCCGG AGGAGAAGGG CCGTCCCGCC CGCTCACCTG TGGGAGTAAC GCGGTCAGTC AGAGCCGGGG CGGGCGGCGC GAGGCGGCGG CGGAGCGGGG CACGGGGGGA AGGCAGCGCG CAACGACTCC CGCCCGCCGC GCGCTTCGCT TTTTATAGGG CCGCCGCCGC CGCCGCCTCG CCATAAAAGG AAACTTTCGG AGCGCGCCGC TCTGATTGGC TGCCGCCGCA CCTTTCCGCC TCGCCCCGCC CCGCCCCTCG CCCCGCCCCG CCCCGCCTGG GGGGGGCCCC CCCCCCCCCC CCGCCCCCAT CGCTGCACAA AATAATTAAA AAATAAATAA ATACAAAATT GGGGGTGGGG AGGGGGGGGA GATGGGGAGA GTGAAGCAGA ACGTGGGGCT CACCTCGACC ATGGTAATAG CGATGACTAA TACGTAGATG TACTGCCAAG TAGGAAAGTC CCATAAGGTC ATGTACTGGG CATAATGCCA GGCGGGCCAT TTACCGTCAT TGACGTCAAT AGGGGGCGTA CTTGGCATAT GATACACTTG ATGTACTGCC AAGTGGGCAG TTTACCGTAA ATACTCCACC CATTGACGTC AATGGAAAGT CCCTATTGGC GTTACTATGG GAACATACGT CATTATTGAC GTCAATGGGC GGGGGTCGTT GGGCGGTCAG CCAGGCGGGC CATTTACCGT AAGTTATGTA ACGCGGAACT CCATATATGG GCTATGAACT AATGACCCCG TAATTGATTA CTATTAATAA CTAGTCAATA ATCAATGTCG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTGGTTTAG TGAACCGTCA GATCCCTGCA GTCGACGGTA CCGCGGGCCC GGGATCCGCC ACCATGTATG TCAAATTGAT ATCATCTGAT GGCCATGAAT TTATTGTAAA AAGAGAACAT GCATTAACAT CAGGCACGAT AAAAGCCATG TTGAGTGGCC CAGGTCAGTT TGCTGAGAAC GAAACCAATG AGGTCAATTT TAGAGAGATA CCTTCACATG TGCTATCGAA AGTATGCATG TATTTTACGT ACAAGGTTCG CTACACTAAC AGCTCCACCG AGATTCCTGA ATTCCCAATT GCACCTGAAA TTGCACTGGA ACTGCTGATG GCTGCGAACT TCTTAGATTG TTACCCATAC GACGTACCTG ATTATGCAAC TTATCCTTAT GATGTACCTG ATTATGCTAG CTATCCTTAT GATGTCCCAG ACTACGCATA GCGGCCGCGA CTCTAGATCA TAATCAGCCA TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTAAGCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTCCTG AGGCGGAAAG AACCAGCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAGATCG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCTAGG GGGAGGCTAA CTGAAACACG GAAGGAGACA ATACCGGAAG GAACCCGCGC TATGACGGCA ATAAAAAGAC AGAATAAAAC GCACGGTGTT GGGTCGTTTG TTCATAAACG CGGGGTTCGG TCCCAGGGCT GGCACTCTGT CGATACCCCA CCGAGACCCC ATTGGGGCCA ATACGCCCGC GTTTCTTCCT TTTCCCCACC CCACCCCCCA AGTTCGGGTG AAGGCCCAGG GCTCGCAGCC AACGTCGGGG CGGCAGGCCC TGCCATAGCC TCAGGTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG T
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