pSuper-H1-Orai1(mouse)-shRNA1-Retro-Puro
$55.00
In stock
SKU
PL24212
-
100μg liquid plasmid(Instantly):$76
| name | pSuper-H1-Orai1(mouse)-shRNA1-Retro-Puro | another name | apSuper-H1-Orai1(mouse)-shRNA1-Retro-Puro |
| Insert Size (bp) | Plasmid host | Mammary cell,retrovirus | |
| Plasmid uses | Interfere with silence | Fragment type | shRNA |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | H1 | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | pSuper.Retro.Puro-stuffer | 5′ sequencing primer | H1 |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TTCCCCCCTT TTTCTGGAGA CTAAATAAAA TCTTTTATTT TATCTATGGC TCGTACTCTA TAGGCTTCAG CTGGTGATAT TGTTGAGTCA AAACTAGAGC CTGGACCACT GATATCCTGT CTTTAACAAA TTGGACTAAT CGCCTCTACG CCGGACGCAT CGTGGCCGGC ATCACCGGCG CCACAGGTGC GGTTGCTGGC GCCTATATCG CCGACATCAC CGATGGGGAA GATCGGGCTC GCCACTTCGG GCTCATGAGC GCTTGTTTCG GCGTGGGTAT GGTGGCAGGC CCCGTGGCCG GGGGACTGTT GGGCGCCATC TCCTTGCATG CACCATTCCT TGCGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATGGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG ATTACGAATT AATTCTCCCC AGCTCTTCTG CGCTGTCCAT AATGAACTAT TTCAGGAGTA TTGGAGTATG TGGATTCAAA ACAGTGGCTA ATTGAGTCAG GATTACTTAA TATTCGACTC CTACCCTCAG AATGTTGTTC TCCAACTTCA AGAAACTGTT ACCCATGAAA GACCCCCGCT GACGGGTAGT CAATCACTCA GAGGAGACCC TCCCAAGGAA CAGCGAGACC ACAAGTCGGA TGCAACTGCA AGAGGGTTTA TTGGATACAC GGGTACCCGG GCGACGCAGT CTATCGGAGG ACTGGCGCGC CGAGTGAGGG GTTGTGGGCT CTTTTATTGA GCTCGGGGAG CAGAAGCGCG CGAACAGAAG CGAGAAGCGA ACTGATTGGT TAGTTCAAAT AAGGCACAGG GTCATTTCAG GTCCTTGGGG CACCCTGGAA ACATCTGATG GTTCTCTAGC TTGCCAAACC TACAGGTGGG GTCTTTCATT CCCCCCTTTT TCTGGAGACT AAATAAAATC TTTTATTTTA TCGATGCATG GGGTCGTGCG CTCCTTTCGG TCGGGCGCTG CGGGTCGTGG GGCGGGCGTC AGGCACCGGG CTTGCGGGTC ATGCACCAGG TGCGCGGTCC TTCGGGCACC TCGACGTCGG CGGTGACGGT GAAGCCGAGC CGCTCGTAGA AGGGGAGGTT GCGGGGCGCG GAGGTCTCCA GGAAGGCGGG CACCCCGGCG CGCTCGGCCG CCTCCACTCC GGGGAGCACG ACGGCGCTGC CCAGACCCTT GCCCTGGTGG TCGGGCGAGA CGCCGACGGT GGCCAGGAAC CACGCGGGCT CCTTGGGCCG GTGCGGCGCC AGGAGGCCTT CCATCTGTTG CTGCGCGGCC AGCCGGGAAC CGCTCAACTC GGCCATGCGC GGGCCGATCT CGGCGAACAC CGCCCCCGCT TCGACGCTCT CCGGCGTGGT CCAGACCGCC ACCGCGGCGC CGTCGTCCGC GACCCACACC TTGCCGATGT CGAGCCCGAC GCGCGTGAGG AAGAGTTCTT GCAGCTCGGT GACCCGCTCG ATGTGGCGGT CCGGATCGAC GGTGTGGCGC GTGGCGGGGT AGTCGGCGAA CGCGGCGGCG AGGGTGCGTA CGGCCCTGGG GACGTCGTCG CGGGTGGCGA GGCGCACCGT GGGCTTGTAC TCGGTCATGG TAAGCTAGCT TGGGCTGCAG GTCGAAAGGC CCGGAGATGA GGAAGAGGAG AACAGCGCGG CAGACGTGCG CTTTTGAAGC GTGCAGAATG CCGGGCCTCC GGAGGACCTT CGGGCGCCCG CCCCGCCCCT GAGCCCGCCC CTGAGCCCGC CCCCGGACCC ACCCCTTCCC AGCCTCTGAG CCCAGAAAGC GAAGGAGCAA AGCTGCTATT GGCCGCTGCC CCAAAGGCCT ACCCGCTTCC ATTGCTCAGC GGTGCTGTCC ATCTGCACGA GACTAGTGAG ACGTGCTACT TCCATTTGTC ACGTCCTGCA CGACGCGAGC TGCGGGGCGG GGGGGAACTT CCTGACTAGG GGAGGAGTAG AAGGTGGCGC GAAGGGGCCA CCAAAGAACG GAGCCGGTTG GCGCCTACCG GTGGATGTGG AATGTGTGCG AGGCCAGAGG CCACTTGTGT AGCGCCAAGT GCCCAGCGGG GCTGCTAAAG CGCATGCTCC AGACTGCCTT GGGAAAAGCG CCTCCCCTAC CCGGTAGAAT TCGAACGCTG ACGTCATCAA CCCGCTCCAA GGAATCGCGG GCCCAGTGTC ACTAGGCGGG AACACCCAGC GCGCGTGCGC CCTGGCAGGA AGATGGCTGT GAGGGACAGG GGAGTGGCGC CCTGCAATAT TTGCATGTCG CTATGTGTTC TGGGAAATCA CCATAAACGT GAAATGTCTT TGGATTTGGG AATCTTATAA GTTCTGTATG AGACCACAGA TCTGCACAAC CTCAACTCGG TCAAATTCAA GAGATTTGAC CGAGTTGAGG TTGTGTTTTT TAAGCTTATC GATACCGTCG ACCTCGAGAG ATCGATCTAA TTCCGGCGCC TAGAGAAGGA GTGAGGGCTG GATAAAGGGA GGATCGAGGC GGGGTCGAAC GAGGAGGTTC AAGGGGGAGA GACGGGGCGG ATGGAGGAAG AGGAGGCGGA GGCTTAGGGT GTACAAAGGG CTTGACCCAG GGAGGGGGGT CAAAAGCCAA GGCTTCCCAG GTCACGATGT AGGGGACCTG GTCTGGGTGT CCATGCGGGC CAGGTGAAAA GACCTTGATC TTAACCTGGG TGATGAGGTC TCGGTTAAAG GTGCCGTCTC GCGGCCATCC GACGTTAAAG GTTGGCCATT CTGCAGAGCA GAAGGTAACC CAACGTCTCT TCTTGACATC TACCGACTGG TTGTGAGCGA TCCGCTCGAC ATCTTTCCAG TGACCTAAGG TCAAACTTAA GGGAGTGGTA ACAGTCTGGC CCTAATTTTC AGACAAATAC AGAAACACAG TCAGACAGAG ACAACACAGA ACGATGCTGC AGCGCTGCAG CAGACAAGAC GCGCGGCTTC GGTTCCAAAC CGAAAGCAAA AATTCAGACG GAGGCGGGAA CTGTTTTAGG TTCTCGTCTC CTACCAGAAC CACATATCCT GACGGGGTCG GATTCCACAT CGACTCCCTT CCTCAGGTCG GGCCACAAAA ACGGCCCCCA AAGTCCCTGG GACGTCTCCC AGGGTTGCGG CCGGGTGTTC AGAACTCGTC AGTTCCACCA CGGGTCCGCC AGATACAGAG CTAGTTAGCT AACTAGTACA GACGCAGGCG CAAACATTAG ATGCCGGCAC AAACACGCAC AAAGACAGAG ACAGACACGA AACGACCGCT GGCCAGCTTA CCTCCCGGCG GGGGGGTCGG TGGTCCCTGG GCAGGGGTCT CCAAATCTCG GTGGAACCTC CAAATGAAAG ACCCCCGAGG TGGGCAGTCA ATCAATCTGA GGAGACCCTC CCAAGGATCA GCGAGTCCAC GATTCGGATG CAAACAGCAA GAGGCTTTAT TGGGAATACG GGTACCCGGG CGACGCAGTC TATCGGAGGA CTGGCGCGCC GAGTGAGGGG TTGTGGGCTC TTTTATTGAG CTCGGGGAGC AGAAGCGCGC GAACAGAAGC GAGAAGCGAA CTGATTGGTT AGTTCAAATA AGGCACAGGG TCATTTCAGG TCCTTGGGGC ACCCTGGAAA CATCTGATGG TTCTCTAGAA ACTGCTGAGG GCGGGACCGC ATCTGGGGAC CATCTGTTCT TGGCCCTGAG CCGGGGCAGG AACTGCTTAC CACAGATATC CTGTTTGGCC CATATTCTGC TGTCTCTCTG TTCCTAACCT TGATCTGAAC TTCTCTATTC TCAGTTATGT ATTTTCCATG CCTTGCAAAA TGGCGTTACT TAAGCTAGCT TGCCAAACCT ACAGGTGGGG TCTTTCA
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