NM_005245.4,FAT1/CDHF7/CDHR8/FAT/hFat1/ME5
100μg liquid plasmid(Instantly):$134
| name | pCMV-FAT1(human-Toxic-opt)(1-1638aa)-TM2-NES-P2A-mCherry-Neo | another name | apCMV-FAT1(human-Toxic-opt)(1-1638aa)-TM2-NES-P2A-mCherry-Neo |
| Insert Size (bp) | 4914bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | Red |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl2 | Growth Temperature | 30°C |
| Vector backbone | pCMV-MCS-TM2-NES-P2A-mCherry-Neo | 5′ sequencing primer | CMV-F |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGACACC GGGACCGATC CAGCCTCCGG ACTCTAGAGC TAGCGTTTAA ACTTAAGCTT GCCACCATGG GGAGACATTT GGCTTTGCTC CTGCTTCTGC TCCTTCTCTT CCAACATTTT GGAGACAGTG ATGGCAGCCA ACGACTTGAA CAGACTCCTC TGCAGTTTAC ACACCTCGAG TACAACGTCA CCGTGCAGGA GAACTCTGCA GCTAAGACTT ATGTGGGGCA TCCTGTCAAG ATGGGTGTTT ACATTACACA TCCAGCGTGG GAAGTAAGGT ACAAAATTGT TTCCGGAGAC AGTGAAAACC TGTTCAAAGC TGAAGAGTAC ATTCTCGGAG ACTTTTGCTT TCTAAGAATA AGGACCAAAG GAGGAAATAC AGCTATTCTT AATAGAGAAG TGAAGGATCA CTACACATTG ATAGTGAAAG CACTTGAAAA AAATACTAAT GTGGAGGCGC GAACAAAGGT CAGGGTGCAG GTGCTGGATA CAAATGACTT GAGACCGTTA TTCTCACCCA CCTCATACAG CGTTTCTTTA CCTGAAAACA CAGCTATAAG GACCAGTATC GCAAGAGTCA GCGCCACGGA TGCAGACATA GGAACCAACG GGGAATTTTA CTACAGTTTT AAAGATCGAA CAGATATGTT TGCTATTCAC CCAACCAGTG GTGTGATAGT GTTAACTGGT AGACTTGATT ACCTAGAGAC CAAGCTCTAT GAGATGGAAA TCCTCGCTGC GGACCGTGGC ATGAAGTTGT ATGGGAGCAG TGGCATCAGC AGCATGGCCA AGCTAACGGT GCACATCGAA CAGGCCAATG AATGTGCTCC GGTGATAACA GCAGTGACAT TGTCACCATC AGAACTGGAC AGGGACCCAG CATATGCAAT TGTGACAGTG GATGACTGCG ATCAGGGTGC CAATGGTGAC ATAGCATCTT TAAGCATCGT GGCAGGTGAC CTTCTCCAGC AGTTTAGAAC AGTGAGGTCC TTTCCAGGGA GTAAGGAGTA TAAAGTCAAA GCCATCGGTG GCATTGATTG GGACAGTCAT CCTTTCGGCT ACAATCTCAC ACTACAGGCT AAAGATAAAG GAACTCCGCC CCAGTTCTCT TCTGTTAAAG TCATTCACGT GACTTCTCCA CAGTTCAAAG CCGGGCCAGT CAAGTTTGAA AAGGATGTTT ACAGAGCAGA AATAAGTGAA TTTGCTCCTC CCAACACACC TGTGGTCATG GTAAAGGCCA TTCCTGCTTA TTCCCATTTG AGGTATGTTT TTAAAAGTAC ACCTGGAAAA GCTAAATTCA GTTTAAATTA CAACACTGGT CTCATTTCTA TTTTAGAACC AGTTAAAAGA CAGCAGGCAG CCCATTTTGA ACTTGAAGTA ACAACAAGTG ACAGAAAAGC GTCCACCAAG GTCTTGGTGA AAGTCTTAGG TGCAAATAGC AATCCCCCTG AATTTACCCA GACAGCGTAC AAAGCTGCTT TTGATGAGAA CGTGCCCATT GGTACTACTG TCATGAGCCT GAGTGCCGTA GACCCTGATG AGGGTGAGAA CGGGTACGTG ACATACAGTA TCGCAAATTT AAATCATGTG CCGTTTGCGA TTGACCATTT CACTGGTGCC GTGAGTACGT CAGAAAACCT GGACTACGAA CTGATGCCTC GGGTTTATAC TCTGAGGATT CGTGCATCAG ACTGGGGCTT GCCGTACCGC CGGGAAGTCG AAGTCCTTGC TACAATTACT CTCAATAACT TGAATGACAA CACACCTTTG TTTGAGAAAA TAAATTGTGA AGGGACAATT CCCAGAGATC TAGGCGTGGG AGAGCAAATA ACCACTGTTT CTGCTATTGA TGCAGATGAA CTTCAGTTGG TACAGTATCA GATTGAAGCT GGAAATGAAC TGGATTTCTT TAGTTTAAAC CCCAACTCGG GGGTATTGTC ATTAAAGCGA TCGCTAATGG ATGGCTTAGG TGCAAAGGTG TCTTTCCACA GTCTGAGAAT CACAGCTACA GATGGAGAAA ATTTTGCCAC ACCATTATAT ATCAACATAA CAGTGGCTGC CAGTCACAAG CTGGTAAACT TGCAGTGTGA AGAGACTGGT GTTGCCAAAA TGCTGGCAGA GAAGCTCCTG CAGGCAAATA AATTACACAA CCAGGGAGAG GTGGAGGATA TTTTCTTCGA TTCTCACTCT GTCAATGCTC ACATACCGCA GTTTAGAAGC ACTCTTCCGA CTGGTATTCA GGTAAAGGAA AACCAGCCTG TGGGTTCCAG TGTAATTTTC ATGAACTCCA CTGACCTTGA CACTGGCTTC AATGGAAAAC TGGTCTATGC TGTTTCTGGA GGAAATGAGG ATAGTTGCTT CATGATTGAC ATGGAAACAG GAATGCTTAA AATTCTCTCC CCACTGGACA GGGAGACCAC GGACAAGTAT ACCTTGAACA TCACCGTTTA TGATCTGGGA ATCCCACAAA AGGCCGCATG GAGGCTCTTG CACGTGGTTG TAGTTGACGC CAACGATAAC CCCCCCGAGT TCCTGCAGGA AAGTTACTTC GTGGAAGTTT CAGAGGACAA GGAGGTTCAC TCTGAAATCA TTCAGGTTGA GGCCACAGAT AAAGATCTTG GGCCCAACGG GCATGTGACC TACTCTATTG TGACTGACAC TGACACCTTC AGCATAGACT CCGTCACAGG CGTGGTGAAT ATCGCCCGGC CACTCGATCG AGAATTGCAA CATGAACATT CTCTTAAGAT TGAAGCCCGC GATCAGGCCA GAGAGGAACC TCAATTGTTT TCCACCGTGG TGGTAAAGGT GAGCCTTGAA GATGTGAACG ACAATCCTCC CACCTTCATT CCCCCAAATT ATCGGGTCAA GGTAAGGGAA GATCTCCCCG AGGGCACAGT GATCATGTGG CTCGAAGCTC ATGACCCAGA TCTGGGACAG TCCGGACAGG TTAGATACTC CCTGCTCGAC CACGGAGAAG GCAACTTTGA TGTGGATAAA CTGTCAGGTG CGGTACGCAT TGTTCAGCAG TTGGATTTCG AGAAGAAGCA GGTTTACAAC CTCACAGTGC GGGCAAAGGA CAAAGGGAAG CCGGTGAGTC TCTCTTCCAC TTGTTACGTG GAGGTGGAAG TCGTTGACGT AAACGAGAAC CTCCACCCAC CCGTCTTCAG CAGTTTTGTG GAGAAAGGCA CAGTTAAGGA AGATGCCCCT GTAGGTAGTC TGGTTATGAC GGTGTCGGCT CATGATGAGG ACGCCAGAAG AGATGGGGAG ATCCGATACT CCATTAGAGA TGGCTCTGGC GTTGGTGTTT TCAAAATAGG TGAAGAGACA GGTGTCATAG AGACGTCAGA TCGACTGGAC CGTGAATCGA CCTCCCATTA TTGGCTAACA GTCTTTGCAA CCGATCAGGG TGTCGTGCCT CTTTCATCGT TCATAGAGAT CTACATAGAG GTTGAGGATG TCAATGACAA TGCACCACAG ACATCAGAGC CTGTTTATTA CCCAGAAATC ATGGAAAATT CTCCTAAAGA TGTATCTGTG GTCCAGATCG AGGCATTTGA TCCAGATTCG AGCTCTAATG ACAAGCTCAT GTACAAAATT ACAAGTGGAA ATCCACAAGG ATTCTTTTCA ATACATCCTA AAACAGGTCT CATCACAACT ACGTCAAGGA AGCTAGACCG AGAACAGCAA GATGAACACA TATTAGAGGT TACTGTGACA GACAATGGTA GTCCCCCCAA ATCAACCATT GCAAGAGTCA TTGTGAAAAT CCTTGATGAA AATGACAACA AACCTCAGTT TCTGCAAAAG TTCTACAAAA TCAGACTCCC TGAGCGGGAA AAGCCAGACC GAGAAAGAAA TGCCAGACGG GAGCCGCTCT ATCACGTCAT AGCCACCGAC AAGGATGAGG GCCCCAATGC AGAAATCTCC TACAGCATCG AAGACGGGAA TGAGCATGGC AAATTTTTCA TCGAACCGAA AACTGGAGTG GTTTCGTCCA AGAGGTTTTC AGCAGCTGGA GAATATGATA TTCTTTCAAT TAAGGCAGTT GACAATGGTC GCCCTCAAAA GTCATCAACC ACCAGACTCC ATATTGAATG GATCTCCAAG CCCAAACCGT CCCTGGAGCC CATTTCATTT GAAGAATCAT TTTTTACCTT TACTGTGATG GAAAGTGACC CCGTTGCTCA CATGATTGGA GTAATATCTG TGGAGCCTCC TGGCATACCC CTTTGGTTTG ACATCACTGG TGGCAACTAC GACAGTCACT TCGATGTGGA CAAGGGAACT GGAACCATCA TTGTTGCCAA ACCTCTTGAT GCAGAACAGA AGTCAAACTA CAACCTCACA GTCGAGGCTA CAGATGGAAC CACCACTATC CTCACTCAGG TATTCATCAA AGTAATAGAC ACAAATGACC ATCGTCCTCA GTTTTCTACA TCAAAGTATG AAGTTGTTAT TCCTGAAGAT ACAGCGCCAG AAACAGAAAT TTTGCAAATC AGTGCTGTGG ATCAGGATGA GAAAAACAAA CTAATCTACA CTCTGCAGAG CAGTAGAGAT CCACTGAGTC TCAAGAAATT TCGTCTTGAT CCTGCAACCG GCTCTCTCTA TACTTCTGAG AAACTGGATC ATGAAGCTGT TCACCAGCAC ACCCTCACGG TCATGGTACG AGATCAAGAT GTGCCTGTAA AACGCAACTT TGCAAGGATT GTGGTCAATG TCAGCGACAC GAATGACCAC GCCCCGTGGT TCACCGCTTC CTCCTACAAA GGGCGGGTTT ATGAATCGGC AGCCGTTGGC TCAGTTGTGT TGCAGGTGAC GGCTCTGGAC AAGGACAAAG GGAAAAATGC TGAAGTGCTG TACTCGATCG AGTCAGGAAA TATTGGAAAT TCTTTTATGA TTGATCCTGT CTTGGGCTCT ATTAAAACTG CCAAAGAATT AGATCGAAGT AACCAAGCGG AGTATGATTT AATGGTAAAA GAATTCATGA TTCTCTGGGA AAAGAAAAGC GAAGATATTA CCGAGACATT CAACCCCGAC AACAACATGA TCGCCGCCGA CGCGAGCGAG GCATCGGAGG CGGGGAAGGC AGAAAACACC CATTACACCG GCAAGCACCG CTTCATGTAC CGGATCCTGT TCGTCCTGAC CTGCCTGATC ACCTTCATTG TGGTGGCTAT TGTCGGCGGA ATTATCGCTT CGAACAGAAA CGTCGGAAAT AGAATAGCTC AAGGCCAAGG ATTAGCCTTG AAATTAGCAG GTCTTGATAT CGGAAGCGGA GCTACTAACT TCAGCCTGCT GAAGCAGGCT GGCGACGTGG AGGAGAACCC TGGACCTATG GTGAGCAAGG GCGAGGAGGA TAACATGGCC ATCATCAAGG AGTTCATGCG CTTCAAGGTG CACATGGAGG GCTCCGTGAA CGGCCACGAG TTCGAGATCG AGGGCGAGGG CGAGGGCCGC CCCTACGAGG GCACCCAGAC CGCCAAGCTG AAGGTGACCA AGGGTGGCCC CCTGCCCTTC GCCTGGGACA TCCTGTCCCC TCAGTTCATG TACGGCTCCA AGGCCTACGT GAAGCACCCC GCCGACATCC CCGACTACTT GAAGCTGTCC TTCCCCGAGG GCTTCAAGTG GGAGCGCGTG ATGAACTTCG AGGACGGCGG CGTGGTGACC GTGACCCAGG ACTCCTCCCT GCAGGACGGC GAGTTCATCT ACAAGGTGAA GCTGCGCGGC ACCAACTTCC CCTCCGACGG CCCCGTAATG CAGAAGAAGA CCATGGGCTG GGAGGCCTCC TCCGAGCGGA TGTACCCCGA GGACGGCGCC CTGAAGGGCG AGATCAAGCA GAGGCTGAAG CTGAAGGACG GCGGCCACTA CGACGCTGAG GTCAAGACCA CCTACAAGGC CAAGAAGCCC GTGCAGCTGC CCGGCGCCTA CAACGTCAAC ATCAAGTTGG ACATCACCTC CCACAACGAG GACTACACCA TCGTGGAACA GTACGAACGC GCCGAGGGCC GCCACTCCAC CGGCGGCATG GACGAGCTGT ACAAGTAACT CGAGTCTAGA GGGCCCCTTA AGGGTTCGAT CCCTACCGGT TAGTAATGAG TTTGATATCT CGACAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAG CTGACGTCCT TTCCATGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCCTGGAAA CGGGGGAGGC TAACTGAAAC ACGGAAGGAG ACAATACCGG AAGGAACCCG CGCTATGACG GCAATAAAAA GACAGAATAA AACGCACGGG TGTTGGGTCG TTTGTTCATA AACGCGGGGT TCGGTCCCAG GGCTGGCACT CTGTCGATAC CCCACCGAGA CCCCATTGGG GCCAATACGC CCGCGTTTCT TCCTTTTCCC CACCCCACCC CCCAAGTTCG GGTGAAGGCC CAGGGCTCGC AGCCAACGTC GGGGCGGCAG GCCCTGCCAT AGCAGATCTG CGCAGCTGGG GCTCTAGGGG GTATCCCCAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGCATCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGG GGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTAAT TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCAATGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCGACGGA TCGGGAGATC TCCCGATCCC CTATGGTCGA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATCTGCTC CCTGCTTGTG TGTTGGAGGT CGCTGAGTAG TGCGCGAGCA AAATTTAAGC TACAACAAGG CAAGGCTTGA CCGACAATTG CATGAAGAAT CTGCTTAGG
