pENTER-CMV-MR1(human)-FLAG-6×His-Puro
$70.00
In stock
SKU
PL23978
NM_001531;HLALS;
100μg liquid plasmid(Instantly):$91
| name | pENTER-CMV-MR1(human)-FLAG-6×His-Puro | another name | apENTER-CMV-MR1(human)-FLAG-6×His-Puro |
| Insert Size (bp) | 1023bp | Plasmid host | Mammary cell,Adeno-associated virus |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Kan |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | pENTER-FLAG-6×His | 5′ sequencing primer | CMV-F |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TTCGAATTTA AATTAATTAA CATCATCAAT AATATACCTT ATTTTGGATT GAAGCCAATA TGATAATGAG GGGGTGGAGT TTGTGACGTG GCGCGGGGCG TGGGAACGGG GCGGGTGACG TAGTAGTGTG GCGGAAGTGT GATGTTGCAA GTGTGGCGGA ACACATGTAA GCGACGGATG TGGCAAAAGT GACGTTTTTG GTGTGCGCCG GTGTACACAG GAAGTGACAA TTTTCGCGCG GTTTTAGGCG GATGTTGTAG TAAATTTGGG CGTAACCGAG TAAGATTTGG CCATTTTCGC GGGAAAACTG AATAAGAGGA AGTGAAATCT GAATAATTTT GTGTTACTCA TAGCGCGTAA TACTGTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTATG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACATCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAC AACTCCGCCC CATTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGTAATACG ACTCACTATA GGGAGAGGAT CCGGTACCGA GGAGATCTGC CGCCGCGATC GCCGGCGCGC CAGATCTCAA GCTTGCCACC ATGGGGGAAC TGATGGCGTT CCTGTTACCT CTCATCATTG TGTTAATGGT GAAGCACAGC GATTCCCGGA CGCACTCTCT GAGATATTTT CGCCTGGGCG TTTCGGATCC CATCCATGGG GTCCCTGAAT TTATTTCGGT TGGGTACGTG GACTCGCACC CTATCACCAC ATATGACAGT GTCACTCGGC AGAAGGAGCC ACGGGCCCCA TGGATGGCAG AGAACCTCGC GCCTGATCAC TGGGAGAGGT ACACTCAGCT GCTGAGGGGC TGGCAGCAGA TGTTCAAGGT GGAACTGAAG CGCCTACAGA GGCACTACAA TCACTCAGGG TCTCACACTT ACCAGAGAAT GATTGGCTGT GAGCTGCTGG AGGATGGAAG CACCACAGGA TTTCTGCAGT ATGCATATGA CGGGCAGGAT TTCCTGATCT TCAATAAAGA CACCCTCTCC TGGCTGGCTG TAGATAATGT GGCTCACACC ATCAAGCAGG CATGGGAGGC CAATCAGCAT GAGTTGCTGT ATCAAAAGAA TTGGCTGGAA GAAGAATGTA TTGCCTGGCT AAAGAGATTC CTGGAGTATG GGAAAGACAC CCTACAAAGA ACAGAGCCCC CACTGGTCAG AGTAAATCGC AAAGAAACTT TTCCAGGGGT TACAGCTCTC TTCTGCAAAG CTCATGGCTT TTACCCCCCA GAAATTTACA TGACATGGAT GAAAAACGGG GAAGAAATTG TCCAAGAAAT TGATTATGGA GACATTCTTC CCAGTGGGGA TGGAACCTAT CAGGCGTGGG CATCAATTGA GCTTGATCCT CAGAGCAGCA ACCTTTACTC CTGTCATGTG GAGCACTGCG GTGTCCACAT GGTTCTTCAG GTCCCCCAGG AATCAGAAAC TATCCCTCTT GTGATGAAAG CTGTCTCTGG GTCCATTGTC CTTGTCATTG TGCTGGCTGG AGTTGGTGTT CTAGTCTGGA GAAGAAGGCC CCGAGAGCAA AATGGAGCCA TCTACCTTCC AACACCAGAT CGACTCGAGG ATTATAAGGA TGACGACGAT AAATTCGTCG AGCACCACCA CCACCACCAC TAATAAGGTT TATCCGATCC ACCGGATCTA GATAAGATAT CCGATCCACC GGATCTAGAT AACTGATCAT AATCAGCCAT ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TAACGCGGAT CTGGGCGTGG TTAAGGGTGG GAAAGAATAT ATAAGGTGGG GGTCTTATGT AGTTTTGTAT CTGTTTTGCA GCAGCCGCCG CCGCCATGAG CACCAACTCG TTTGATGGAA GCATTGTGAG CTCATATTTG ACAACGCGCA TGCCCCCATG GGCCGGGGTG CGTCAGAATG TGATGGGCTC CAGCATTGAT GGTCGCCCCG TCCTGCCCGC AAACTCTACT ACCTTGACCT ACGAGACCGT GTCTGGAACG CCGTTGGAGA CTGCAGCCTC CGCCGCCGCT TCAGCCGCTG CAGCCACCGC CCGCGGGATT GTGACTGACT TTGCTTTCCT GAGCCCGCTT GCAAGCAGTG CAGCTTCCCG TTCATCCGCC CGCGATGACA AGTTGACGGC TCTTTTGGCA CAATTGGATT CTTTGACCCG GGAACTTAAT GTCGTTTCTC AGCAGCTGTT GGATCTGCGC CAGCAGGTTT CTGCCCTGAA GGCTTCCTCC CCTCCCAATG CGGTTTAAAA CATAAATAAA AAACCAGACT CTGTTTGGAT TTGGATCAAG CAAGTGTCTT GCTGTCTTTA TTTAGGGGTT TTGCGCGCGC GGTAGGCCCG GGACCAGCGG TCTCGGTCGT TGAGGGTCCT GTGTATTTTT TCCAGGACGT GGTAAAGGTG ACTCTGGATG TTCAGATACA TGGGCATAAG CCCGTCTCTG GGGTGGAGGT AGCACCACTG CAGAGCTTCA TGCTGCGGGG TGGTGTTGTA GATGATCCAG TCGTAGCAGG AGCGCTGGGC GTGGTGCCTA AAAATGTCTT TCAGTAGCAA GCTGATTGCC AGGGGCAGGC CCTTGGTGTA AGTGTTTACA AAGCGGTTAA GCTGGGATGG GTGCATACGT GTTTAAACTA GGGATAACAG GGTAATCGGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACACGTA CGACCATGAC CGAGTACAAG CCCACGGTGC GCCTCGCCAC CCGCGACGAC GTCCCCCGGG CAGTACGCAC CCTCGCCGCC GCGTTCGCCG ACTACCCCGC CACGCGCCAC ACCGTCGATC CAGACCGCCA CATCGAGCGG GTCACCGAGC TGCAAGAACT CTTCCTCACG CGCGTCGGGC TCGACATCGG CAAGGTGTGG GTCGCGGACG ACGGCGCCGC GGTGGCGGTC TGGACCACGC CGGAGAGCGT CGAAGCGGGG GCGGTGTTCG CCGAGATCGG CCCGCGCATG GCCGAGTTGA GCGGTTCCCG GCTGGCCGCG CAGCAACAGA TGGAAGGCCT CCTGGCGCCG CACCGGCCCA AGGAGCCCGC GTGGTTCCTG GCCACCGTCG GCGTCTCGCC CGACCACCAG GGCAAGGGTC TGGGCAGCGC CGTCGTGCTC CCCGGAGTGG AGGCGGCCGA GCGCGCCGGG GTGCCCGCCT TCCTGGAGAC CTCCGCGCCC CGCAACCTCC CCTTCTACGA GCGGCTCGGC TTCACCGTCA CCGCCGACGT CGAGGTGCCC GAAGGACCGC GCACCTGGTG CATGACCCGC AAGCCCGGTG CCTGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG GGATGCGGTG GGCTCTATGG TAGGGATAAC AGGGTAATGT TTAAACTATA AAATGCAAGG TGCTGCTCAA AAAATCAGGC AAAGCCTCGC GCAAAAAAGA AAGCACATCG TAGTCATGCT CATGCAGATA AAGGCAGGTA AGCTCCGGAA CCACCACAGA AAAAGACACC ATTTTTCTCT CAAACATGTC TGCGGGTTTC TGCATAAACA CAAAATAAAA TAACAAAAAA ACATTTAAAC ATTAGAAGCC TGTCTTACAA CAGGAAAAAC AACCCTTATA AGCATAAGAC GGACTACGGC CATGCCGGCG TGACCGTAAA AAAACTGGTC ACCGTGATTA AAAAGCACCA CCGACAGCTC CTCGGTCATG TCCGGAGTCA TAATGTAAGA CTCGGTAAAC ACATCAGGTT GATTCACATC GGTCAGTGCT AAAAAGCGAC CGAAATAGCC CGGGGGAATA CATACCCGCA GGCGTAGAGA CAACATTACA GCCCCCATAG GAGGTATAAC AAAATTAATA GGAGAGAAAA ACACATAAAC ACCTGAAAAA CCCTCCTGCC TAGGCAAAAT AGCACCCTCC CGCTCCAGAA CAACATACAG CGCTTCCACA GCGGCAGCCA TAACAGTCAG CCTTACCAGT AAAAAAGAAA ACCTATTAAA AAAACACCAC TCGACACGGC ACCAGCTCAA TCAGTCACAG TGTAAAAAAG GGCCAAGTGC AGAGCGAGTA TATATAGGAC TAAAAAATGA CGTAACGGTT AAAGTCCACA AAAAACACCC AGAAAACCGC ACGCGAACCT ACGCCCAGAA ACGAAAGCCA AAAAACCCAC AACTTCCTCA AATCGTCACT TCCGTTTTCC CACGTTACGT CACTTCCCAT TTTAAGAAAA CTACAATTCC CAACACATAC AAGTTACTCC GCCCTAAAAC CTACGTCACC CGCCCCGTTC CCACGCCCCG CGCCACGTCA CAAACTCCAC CCCCTCATTA TCATATTGGC TTCAATCCAA AATAAGGTAT ATTATTGATG ATGTTAATTA ATTTAAATTC GAACATGCAT GGATCCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTG CAGCCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTCACGTAG AAAGCCAGTC CGCAGAAACG GTGCTGACCC CGGATGAATG TCAGCTACTG GGCTATCTGG ACAAGGGAAA ACGCAAGCGC AAAGAGAAAG CAGGTAGCTT GCAGTGGGCT TACATGGCGA TAGCTAGACT GGGCGGTTTT ATGGACAGCA AGCGAACCGG AATTGCCAGC TGGGGCGCCC TCTGGTAAGG TTGGGAAGCC CTGCAAAGTA AACTGGATGG CTTTCTTGCC GCCAAGGATC TGATGGCGCA GGGGATCAAG CTCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCCG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAA TTTTGTTAAA ATTTTTGTTA AATCAGCTCA TTTTTTAACC AATAGGCCGA AATCGGCAAA ATCCCTTATA AATCAAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC AAGAGTCCAC TATTAAAGAA CGTGGACTCC AACGTCAAAG GGCGAAAAAC CGTCTATCAG GGCGATGGCC CACTACGTGA ACCATCACCC TAATCAAGTT TTTTGGGGTC GAGGTGCCGT AAAGCACTAA ATCGGAACCC TAAAGGGAGC CCCCGATTTA GAGCTTGACG GGGAAAGCCG GCGAACGTGG CGAGAAAGGA AGGGAAGAAA GCGAAAGGAG CGGGCGCTAG GGCGCTGGCA AGTGTAGCGG TCACGCTGCG CGTAACCACC ACACCCGCCG CGCTTAATGC GCCGCTACAG GGCGCGTCCA TTCGCCATTC AGGATCGAAT TAATTC
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