pCMV-EGFP-ATP7A(human)-3×FLAG-Puro
$113.00
In stock
SKU
PL23620
-
100μg liquid plasmid(Instantly):$134
| name | pCMV-EGFP-ATP7A(human)-3×FLAG-Puro | another name | apCMV-EGFP-ATP7A(human)-3×FLAG-Puro |
| Insert Size (bp) | 4500bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | EPI400 | Growth Temperature | 30°C |
| Vector backbone | pCMV-EGFP-MCS-3×FLAG-Puro | 5′ sequencing primer | pEGFP-C-5' |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC AAGCTTTCTA GAGAATTCGA TATCGGATCC GCCACCATGG TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGGGCGGTG GAGGCAGTGG TGGAGGCGGT AGCGGTACCA TGGATCCAAG TATGGGTGTG AATTCTGTTA CCATTTCTGT TGAGGGTATG ACTTGCAATT CCTGTGTTTG GACCATTGAG CAGCAGATTG GAAAAGTGAA TGGTGTGCAT CACATTAAGG TATCACTGGA AGAAAAAAAT GCAACTATTA TTTATGACCC TAAACTACAG ACTCCAAAGA CCCTACAGGA AGCTATTGAT GACATGGGCT TTGATGCTGT TATCCATAAT CCTGACCCTC TCCCTGTTTT AACTGACACC TTGTTTCTGA CTGTTACGGC GTCACTGACT TTGCCATGGG ACCATATCCA AAGCACATTG CTGAAGACCA AGGGTGTGAC AGACATTAAA ATTTACCCTC AGAAAAGAAC TGTAGCAGTG ACAATAATCC CTTCTATAGT GAATGCCAAT CAGATAAAAG AGCTGGTTCC AGAACTCAGT TTAGATACTG GGACACTGGA GAAAAAGTCA GGAGCTTGTG AAGATCATAG TATGGCTCAA GCTGGTGAAG TCGTGCTGAA GATGAAAGTG GAAGGGATGA CCTGCCATTC ATGTACTAGC ACTATTGAAG GAAAAATTGG GAAACTGCAA GGTGTTCAGC GAATTAAAGT CTCCCTGGAC AATCAAGAAG CTACTATTGT TTATCAACCT CATCTTATCT CAGTAGAGGA AATGAAAAAG CAGATTGAAG CTATGGGCTT TCCAGCATTT GTCAAAAAGC AGCCCAAGTA CCTCAAATTG GGAGCTATTG ATGTAGAACG TCTAAAGAAC ACACCAGTTA AATCCTCAGA AGGGTCACAG CAAAGGAGTC CATCATATAC CAATGATTCA ACAGCCACTT TCATCATTGA TGGCATGCAT TGTAAATCAT GTGTGTCAAA TATTGAAAGT ACTTTATCTG CACTCCAATA TGTAAGCAGC ATAGTAGTTT CTTTAGAGAA TAGGTCTGCC ATTGTGAAGT ATAATGCAAG CTCAGTCACT CCAGAATCCC TGAGAAAAGC AATAGAGGCT GTATCACCGG GGCTATATAG AGTTAGTATC ACAAGTGAAG TTGAGAGTAC CTCAAACTCT CCCTCCAGCT CATCTCTTCA GAAGATTCCT TTGAATGTAG TTAGCCAGCC TCTGACACAA GAAACTGTGA TAAACATTGA TGGCATGACT TGTAATTCCT GTGTGCAGTC TATTGAGGGT GTCATATCAA AAAAGCCAGG TGTAAAATCC ATACGAGTCT CCCTTGCAAA TAGCAATGGG ACTGTTGAGT ATGATCCTCT ACTAACCTCT CCAGAAACGT TGAGAGGAGC AATAGAAGAC ATGGGATTTG ATGCTACCTT GTCAGACACG AATGAGCCGT TGGTAGTAAT AGCTCAGCCT TCATCGGAAA TGCCGCTTTT GACTTCAACT AATGAATTTT ATACTAAAGG GATGACACCA GTTCAAGACA AGGAGGAAGG AAAGAATTCA TCTAAGTGTT ACATACAGGT CACTGGCATG ACTTGCGCTT CCTGTGTAGC AAACATTGAA CGGAATTTAA GGCGGGAAGA AGGAATATAT TCTATACTTG TGGCCCTGAT GGCTGGCAAG GCAGAAGTAA GGTATAATCC TGCTGTTATA CAACCCCCAA TGATAGCAGA GTTCATCCGA GAACTTGGAT TTGGAGCCAC TGTGATAGAA AATGCTGATG AAGGAGATGG TGTTTTGGAA CTTGTTGTGA GGGGAATGAC GTGTGCCTCC TGCGTACATA AAATAGAGTC TAGTCTCACA AAACACAGAG GGATCCTATA CTGCTCCGTG GCCCTGGCAA CCAACAAAGC ACATATTAAA TATGACCCAG AAATTATTGG TCCTAGAGAT ATTATCCATA CAATTGAAAG CTTAGGTTTT GAAGCTTCTT TGGTCAAGAA GGATCGGTCA GCAAGTCACT TAGATCATAA ACGAGAAATA AGACAATGGA GACGGTCTTT TCTTGTGAGT CTGTTTTTCT GTATTCCTGT AATGGGGCTG ATGATATATA TGATGGTTAT GGACCACCAC TTTGCAACTC TTCACCATAA TCAAAACATG AGTAAAGAAG AAATGATCAA CCTTCATTCT TCTATGTTCC TGGAGCGCCA GATTCTTCCA GGATTGTCTG TTATGAATTT GCTGTCCTTT TTATTGTGTG TACCTGTACA GTTTTTCGGA GGCTGGTACT TCTACATTCA GGCTTATAAA GCACTGAAGC ATAAGACAGC AAATATGGAC GTACTGATTG TGCTGGCAAC CACCATTGCA TTTGCCTACT CTTTGATTAT TCTTCTAGTT GCAATGTATG AGAGAGCCAA AGTGAACCCT ATTACTTTCT TTGACACACC CCCTATGCTG TTTGTGTTTA TTGCACTAGG CCGATGGCTG GAACATATAG CAAAGGGCAA AACATCAGAG GCTCTTGCAA AGTTAATTTC ACTACAAGCT ACAGAAGCAA CTATTGTAAC TCTTGATTCT GATAATATCC TCCTCAGTGA AGAACAAGTG GATGTGGAAC TTGTACAACG TGGAGATATC ATTAAAGTAG TTCCAGGAGG CAAATTTCCA GTGGATGGTC GTGTTATTGA AGGACATTCT ATGGTAGATG AGTCCCTCAT CACAGGGGAG GCAATGCCTG TGGCTAAGAA ACCTGGCAGC ACAGTGATTG CTGGTTCCAT TAACCAGAAC GGGTCACTGC TTATCTGCGC AACACATGTT GGAGCAGACA CAACCCTTTC TCAAATTGTC AAACTTGTGG AAGAGGCACA AACATCAAAG GCTCCTATCC AGCAGTTTGC AGACAAACTC AGTGGCTATT TTGTTCCTTT TATTGTTTTT GTTTCCATTG CCACCCTCTT GGTATGGATT GTAATTGGAT TTCTGAATTT TGAAATTGTG GAAACCTACT TTCCTGGCTA CAATAGAAGT ATCTCCCGAA CAGAAACGAT AATACGATTT GCTTTCCAAG CCTCTATCAC AGTTCTGTGT ATTGCATGTC CCTGTTCACT GGGACTGGCC ACTCCAACTG CTGTGATGGT GGGTACAGGA GTAGGTGCTC AAAATGGCAT ACTAATAAAA GGTGGAGAGC CATTGGAGAT GGCTCATAAG GTAAAGGTAG TGGTATTTGA TAAGACTGGA ACCATTACTC ACGGAACCCC AGTGGTGAAT CAAGTAAAGG TTCTAACTGA AAGTAACAGA ATATCACACC ATAAAATCTT GGCCATTGTG GGAACTGCTG AAAGTAACAG TGAACACCCT CTAGGAACAG CCATAACCAA ATATTGCAAA CAGGAGCTGG ACACTGAAAC CTTGGGTACC TGCATAGATT TCCAGGTTGT GCCAGGCTGT GGTATTAGCT GTAAAGTCAC CAATATTGAA GGCTTGCTAC ATAAGAATAA CTGGAATATA GAGGACAATA ATATTAAAAA TGCATCCCTG GTTCAAATTG ATGCCAGTAA TGAACAGTCA TCAACTTCGT CTTCCATGAT TATTGATGCC CAGATCTCAA ATGCTCTTAA TGCTCAGCAG TATAAAGTCC TCATTGGTAA CCGGGAGTGG ATGATTAGAA ATGGTCTTGT CATTAATAAC GATGTAAATG ATTTCATGAC TGAACATGAG AGAAAAGGTC GGACTGCTGT ATTAGTAGCA GTTGATGATG AGCTGTGTGG CTTGATAGCC ATTGCAGACA CAGTGAAGCC TGAAGCAGAA CTGGCTATCC ATATTCTGAA ATCTATGGGC TTAGAAGTAG TTCTGATGAC TGGAGACAAC AGTAAAACAG CTAGATCTAT TGCTTCTCAG GTTGGCATTA CTAAGGTGTT TGCTGAAGTT CTACCTTCTC ACAAGGTTGC TAAAGTGAAG CAACTTCAAG AGGAGGGGAA ACGGGTAGCA ATGGTGGGAG ATGGAATCAA TGACTCCCCA GCTCTGGCAA TGGCTAATGT GGGAATTGCT ATTGGCACAG GCACAGATGT AGCCATTGAA GCAGCTGATG TGGTTTTGAT AAGGAATGAT CTTCTGGATG TAGTGGCAAG TATTGACTTA TCAAGAAAGA CAGTCAAGAG GATTCGGATA AATTTTGTCT TTGCTCTAAT TTATAATCTG GTTGGAATTC CCATAGCTGC TGGAGTTTTT ATGCCCATTG GTTTGGTTTT GCAGCCCTGG ATGGGATCTG CAGCAATGGC TGCTTCATCT GTTTCTGTAG TACTTTCTTC TCTCTTCCTT AAACTTTACA GGAAACCAAC TTACGAGAGT TATGAACTGC CTGCCCGGAG CCAGATAGGA CAGAAGAGTC CTTCAGAAAT CAGCGTTCAT GTTGGAATAG ATGATACCTC AAGGAATTCT CCTAAACTGG GTTTGCTGGA CCGGATTGTT AATTATAGCA GAGCCTCTAT AAACTCACTA CTGTCTGATA AACGCTCCCT AAACAGTGTT GTTACCAGTG AACCTGACAA GCACTCACTC CTGGTGGGAG ACTTCAGGGA AGATGATGAC ACTGCATTAC TCGAGACCGG TGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC ATCGATTACA AGGATGACGA TGACAAGTGA CCGGTAAGTT TAAACCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAT GCGGTGGGCT CTATGGCTTC TGAGGCGGAA AGAACCAGCT GGGGCTCTAG GGGGTATCCC CACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGGCCATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT AATTCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCTGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGATGACC GAGTACAAGC CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCAGGGC CGTACGCACC CTCGCCGCCG CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGATCC GGACCGCCAC ATCGAGCGGG TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG CCACCGTCGG CGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC CCGGAGTGGA GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC GAGGTGCCCG AAGGACCGCG CACCTGGTGC ATGACCCGCA AGCCCGGTGC CTGATTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTC
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