NM_001163470.1;1110008K06Rik; Fln29;
100μg liquid plasmid(Instantly):$91
| name | pLV2-TRE3GS-Trafd1(mouse)-3×FLAG-TetOne-Fluc-Puro | another name | apLV2-TRE3GS-Trafd1(mouse)-3×FLAG-TetOne-Fluc-Puro |
| Insert Size (bp) | 1740bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression,Tetracycline induction | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Fluc |
| Promoter | TRE3GS | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | pLV2-TRE3GS-MCS-3×FLAG-TetOne-Fluc-Puro | 5′ sequencing primer | TRE3GS-F1 |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACTACTAAGT TTGTAGTACA TATTTAACAA ATACAATTTC TTTAAAAACT ACTAAGTTTG TAGTACATAT TTAACAAATA CAATTTCTTT AAAATGAAAA TAATTCAGAG GAATCACAGG TTTAGAGTAA ATGAAACCAC TAGGTAATTG GCAGTGGTAA TAGGGTATGG GGTGGGAAGT TTGGGATGAT TTTGGTTAGC TTGAGTTATC CAGTTGATCC AGACATGATA AGATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT GATCTCCCGA TCCGTCGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTCT AGCTAGAGGT CGACGGTAAA ACGCTAGCAA TGTCAAGGCC TCTCACTCTC TGATATTCAT TTCTTTGCAA GTTATAAATA CTGAATAATA AGATGACATG AACTACTACT GCTAGAGATT TTCCACACTG ACTAAAAGGG TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGGCA AAAAGCAGCT GCTTATATGC AGGATCTGAG GGCTCGCCAC TCCCCAGTCC CGCCCAGGCC ACGCCTCCCT GGAAAGTCCC CAGCGGAAAG TCCCTTGTAG CAAGCTCGAT ATCAGCAGTT CTTGAAGTAC TCCGGATGCA GCTCTCGGGC CACGTGATGA AATGCTAGGC GGCTGTCAAA CCTCCACTCT AACACTTCTC TCTCCGGGTC ATCCATCCCA TGCAGGCTCA CAGGGTGTAA CAAGCTGGTG TTCTCTCCTT TATTGGCCTC TTCTACCTTA TCTGGCTCAA CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA TCTGACCCCT GGCCCTGGTG TGTAGTTCTG CTAATCAGGG AAGTAGCCTT GTGTGTGGTA GATCCACAGA TCAAGGATAT CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCT CTTTTCTTTT AAAAAGTGGC TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG TACCTGAGGT GTGACTGGAA AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA ATCAGCATTG GTAGCTGCTG TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG ACTGCAGAAT TAATTCCAGG CGGGGAGGCG GCCCAAAGGG AGATCCGACT CGTCTGAGGG CGAAGGCGAA GACGCGGAAG AGGCCGCAGA GCCGGCAGCA GGCCGCGGGA AGGAAGGTCC GCTGGATTGA GGGCCGAAGG GACGTAGCAG AAGGACGTCC CGCGCAGAAT CCAGGTGGCA ACACAGGCGA GCAGCCATGG AAAGGACGTC AGCTTCCCCG ACAACACCAC GGAATTGTCA GTGCCCAACA GCCGAGCCCC TGTCCAGCAG CGGGCAAGGC AGGCGGCGAT GAGTTCCGCC GTGGCAATAG GGAGGGGGAA AGCGAAAGTC CCGGAAAGGA GCTGACAGGT GGTGGCAATG CCCCAACCAG TGGGGGTTGC GTCAGCAAAC ACAGTGCACA CCACGCCACG TTGCCTGACA ACGGGCCACA ACTCCTCATA AAGAGACAGC AACCAGGATT TATACAAGGA GGAGAAAATG AAAGCCATAC GGGAAGCAAT AGCATGATAC AAAGGCATTA AAGCAGCGTA TCCACATAGC GTAAAAGGAG CAACATAGTT AAGAATACCA GTCAATCTTT CACAAATTTT GTAATCCAGA GGTTGATTGT TCCAGACGCG TTCAGGCACC GGGCTTGCGG GTCATGCACC AGGTGCGCGG TCCTTCGGGC ACCTCGACGT CGGCGGTGAC GGTGAAGCCG AGCCGCTCGT AGAAGGGGAG GTTGCGGGGC GCGGAGGTCT CCAGGAAGGC GGGCACCCCG GCGCGCTCGG CCGCCTCCAC TCCGGGGAGC ACGACGGCGC TGCCCAGACC CTTGCCCTGG TGGTCGGGCG AGACGCCGAC GGTGGCCAGG AACCACGCGG GCTCCTTGGG CCGGTGCGGC GCCAGGAGGC CTTCCATCTG TTGCTGCGCG GCCAGCCGGG AACCGCTCAA CTCGGCCATG CGCGGGCCGA TCTCGGCGAA CACCGCCCCC GCTTCGACGC TCTCCGGCGT GGTCCAGACC GCCACCGCGG CGCCGTCGTC CGCGACCCAC ACCTTGCCGA TGTCGAGCCC GACGCGCGTG AGGAAGAGTT CTTGCAGCTC GGTGACCCGC TCGATGTGGC GGTCCGGGTC GACGGTGTGG CGCGTGGCGG GGTAGTCGGC GAACGCGGCG GCGAGGGTGC GTACGGCCCT GGGGACGTCG TCGCGGGTGG CGAGGCGCAC CGTGGGCTTG TACTCGGTCA TAGGGCCGGG ATTCTCCTCC ACGTCACCGC ATGTTAGAAG ACTTCCTCTG CCCTCGCGAG ATCCGGTGGA GCCGGGTCCG GCGGTGCCGT CCACGGCAGA ATTGGACGAC TGAGCGCGGG ATCTCACGGC GATCTTTCCG CCCTTCTTGG CCTTTATGAG GATCTCTCTG ATTTTTCTTG CGTCGAGTTT TCCGGTAAGA CCTTTCGGTA CTTCGTCCAC AAACACAACT CCTCCGCGCA ACTTTTTCGC GGTTGTTACT TGACTGGCGA CGTAATCCAC GATCTCTTTT TCCGTCATCG TCTTTCCGTG CTCCAAAACA ACAACGGCGG CGGGAAGTTC ACCGGCGTCA TCGTCGGGAA GACCTGCGAC ACCTGCGTCG AAGATGTTGG GGTGTTGGAG CAAGATGGAT TCCAATTCAG CGGGAGCCAC CTGATAGCCT TTGTACTTAA TCAGAGACTT CAGGCGGTCA ACGATGAAGA AGTGTTCGTC TTCGTCCCAG TAAGCTATGT CTCCAGAATG TAGCCATCCA TCCTTGTCAA TCAAGGCGTT GGTCGCTTCC GGATTGTTTA CATAACCGGA CATAATCATA GGACCTCTCA CACACAGTTC GCCTCTTTGA TTAACGCCCA GCGTTTTCCC GGTATCCAGA TCCACAACCT TCGCTTCAAA AAATGGAACA ACTTTACCGA CCGCGCCCGG TTTATCATCC CCCTCGGGTG TAATCAGAAT AGCTGATGTA GTCTCAGTGA GCCCATATCC TTGCCTGATA CCTGGCAGAT GGAACCTCTT GGCAACCGCT TCCCCGACTT CCTTAGAGAG GGGAGCGCCA CCAGAAGCAA TTTCGTGTAA ATTAGATAAA TCGTATTTGT CAATCAGAGT GCTTTTGGCG AAGAAGGAGA ATAGGGTTGG CACCAGCAGC GCACTTTGAA TCTTGTAATC CTGAAGGCTC CTCAGAAACA GCTCTTCTTC AAATCTATAC ATTAAGACGA CTCGAAATCC ACATATCAAA TATCCGAGTG TAGTAAACAT TCCAAAACCG TGATGGAATG GAACAACACT TAAAATCGCA GTATCCGGAA TGATTTGATT GCCAAAAATA GGATCTCTGG CATGCGAGAA TCTCACGCAG GCAGTTCTAT GAGGCAGAGC GACACCTTTA GGCAGACCAG TAGATCCAGA GGAGTTCATG ATCAGTGCAA TTGTCTTGTC CCTATCGAAG GACTCTGGCA CAAAATCGTA TTCATTAAAA CCGGGAGGTA GATGAGATGT GACGAACGTG TACATCGACT GAAATCCCTG GTAATCCGTT TTAGAATCCA TGATAATAAT TTTTTGGATG ATTGGGAGCT TTTTTTGCAC GTTCAAAATT TTTTGCAACC CCTTTTTGGA AACGAACACC ACGGTAGGCT GCGAAATGCC CATACTGTTG AGCAATTCAC GTTCATTATA AATGTCGTTC GCGGGCGCAA CTGCAACTCC GATAAATAAC GCGCCCAACA CCGGCATAAA GAATTGAAGA GAGTTTTCAC TGCATACGAC GATTCTGTGA TTTGTATTCA GCCCATATCG TTTCATAGCT TCTGCCAACC GAACGGACAT TTCGAAGTAC TCAGCGTAAG TGATGTCCAC CTCGATATGT GCATCTGTAA AAGCAATTGT TCCAGGAACC AGGGCGTATC TCTTCATAGC CTTATGCAGT TGCTCTCCAG CGGTTCCATC TTCCAGCGGA TAGAATGGCG CCGGGCCTTT CTTTATGTTT TTGGCGTCTT CCATCAGGCC GCTCTCGTCG CTCTCCATGG TGGCGTCTAG CGTAGGCGCC GGTCACAGCT TGTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCGCGC GTTTACCCGG GGAGCATGTC AAGGTCAAAA TCGTCAAGAG CGTCAGCAGG CAGCATATCA AGGTCAAAGT CGTCAAGGGC ATCGGCTGGG AGCATGTCTA AGTCAAAATC GTCAAGGGCG TCGGTCGGCC CGCCGCTTTC GCACTTTAGC TGTTTCTCCA GGCCACATAT GATTAGTTCC AGGCCGAAAA GGAAGGCAGG TTCGGCTCCC TGCCGGTCGA ACAGCTCAAT TGCTTGTTTC AGAAGTGGGG GCATAGAATC GGTGGTAGGT GTCTCTCTTT CCTCTTTTGC TACTTGATGC TCCTGTTCCT CCAATACGCA GCCCAGTGTA AAGTGGCCCA CGGCGGACAG AGCGTACAGT GCGTTCTCCA GGGAGAAGCC TTGCTGACAC AGGAACGCGA GCTGATTTTC CAGGGTTTCG TACTGTTTCT CTGTTGGGCG GGTGCCGAGA TGCACTTTAG CCCCGTCGCG ATGTGAGAGG AGAGCACAGC GGTATGACTT GGCGTTGTTC CGCAGAAAGT CTTGCCATGA CTCGCCTTCC AGGGGGCAGG AGTGGGTATG ATGCCTGTCC AGCATCTCGA TTGGCAGGGC ATCGAGCAGG GCCCGCTTGT TCTTCACGTG CCAGTACAGG GTAGGCTGCT CAACTCCCAG CTTTTGAGCG AGTTTCCTTG TCGTCAGGCC TTCGATACCG ACTCCATTGA GTAATTCCAG AGCAGAGTTT ATGACTTTGC TCTTGTCCAG TCTAGACATG GTAATTCGAT GATCCCCCTG GGGAGAGAGG TCGGTGATTC GGTCAACGAG GGAGCCGACT GCCGACGTGC GCTCCGGAGG CTTGCAGAAT GCGGAACACC GCGCGGGCAG GAACAGGGCC CACACTACCG CCCCACACCC CGCCTCCCGC ACCGCCCCTT CCCGGCCGCT GCTCTCGGCG CGCCCTGCTG AGCAGCCGCT ATTGGCCACA GCCCATCGCG GTCGGCGCGC TGCCATTGCT CCCTGGCGCT GTCCGTCTGC GAGGGTACTA GTGAGACGTG CGGCTTCCGT TTGTCACGTC CGGCACGCCG CGAACCGCAA GGAACCTTCC CGACTTAGGG GCGGAGCAGG AAGCGTCGCC GGGGGGCCCA CAAGGGTAGC GGCGAAGATC CGGGTGACGC TGCGAACGGA CGTGAAGAAT GTGCGAGACC CAGGGTCGGC GCCGCTGCGT TTCCCGGAAC CACGCCCAGA GCAGCCGCGT CCCTGCGCAA ACCCAGGGCT GCCTTGGAAA AGGCGCAACC CCAACCCCGT GGAATTATCA CCTCGAGTTT ACTCCCTATC AGTGATAGAG AACGTATGAA GAGTTTACTC CCTATCAGTG ATAGAGAACG TATGCAGACT TTACTCCCTA TCAGTGATAG AGAACGTATA AGGAGTTTAC TCCCTATCAG TGATAGAGAA CGTATGACCA GTTTACTCCC TATCAGTGAT AGAGAACGTA TCTACAGTTT ACTCCCTATC AGTGATAGAG AACGTATATC CAGTTTACTC CCTATCAGTG ATAGAGAACG TATAAGCTTT GCTTATGTAA ACCAGGGCGC CTATAAAAGA GTGCTGATTT TTTGAGTAAA CTTCAATTCC ACAACACTTT TGTCTTATAC CAACTTTCCG TACCACTTCC TACCCTCGTA AAGAATTCGC CACCATGGCC GAGTTTCGAG ATGACCAGGC TTCTAGGCTG TGTGACAACT GCAAGAAGGA AATTCCTGTA TTTAATTTTA CCATCCATGA AATCCACTGT CAAAGGAACA TTGGTGTGTG CCCTGTCTGC AAGGAACCGT TCCCCAAATC TGACATGGAC ATTCACATGG CTGCAGAGCA CTGTCAGGTG ACCTGTAAAT GCAACAAGAA GTTGGAGAAG AGGCAGTTAA AGCAGCATGC GGAGACAGAG TGTCCCCTGC GGCTCGCCGT CTGCCAGCAC TGTGATCTGG AGCTTTCTGT TGTCAAGTTG AAGGAGCATG AGGATTACTG TGGAGCCCGG ACAGAGCTGT GTGGCAGCTG TGGGCGCAAC GTGCTTGTGA AGGAGCTGAA GACTCACCCC GAAGTCTGTG GGAGAGTGGA GGAGGAAAAG AGAACGGAGG CTGCCATCCC TCCGGAGGCT TACGACGAGC CCTGGAGTCA GGACAGAATC TGGATCGCAT CCCAGCTCCT CAGACAAATC GAGGCTCTGG ACCCGCCCAT GAGGCTCCCT GGAAGGCCCC TGCAAGCCTT TGAGGCAGAC CCCTTCTACA GTAGGACTAC CAGCCAGAGG AGCATGGCAG CCCAGTTTCC AGTTCAAAAT AATCTTTTTG AAGAACAAGA AAGGCAGGAA AGGAACAGAA GCCGGCAGTC CCCAAAGGAC AGCGCTGAGA ATAACGCACA CTTGGACTTC ATGTTGGCCT TGAGTCTGCA GAATGAGGGA CAGGCCACCA GCATGGTAGA GCAGGGCTTC TGGGAGTCTG TGCCTGAGGC TGATCCGGCT CGTGCTGGGC CCACATCTCT AGGTGACATA AAGGGTGCTG CTGACGAGAT TCTGCTGCCG TGTGAGTTCT GTGAGGAGCT GTACCCAGAG GAACTGCTCA TTGACCATCA GACAAGCTGC AACCCTTCTC ATGCCTTACG TTCACTCAAT ACGGGCAGCT CTTCCATCAG GGGTGTGGAA GATCCTGGTA CCATCTTCCA GAACTTTCTA CAACAAGCAA CAAGTAACCA GTTTGACACT TTAATGGGCC TGAGCAGTTC AGCTGCTGTG GAAGACAGCA TCATCATCCC CTGTGAGTTC TGTGGGGTGC AGCTGGAAGA GGAGGTGCTG TTCTACCATC AGGACCAGTG TGACCAACGC CCAGCCACAG CAAACCACCG TGCAGTGGAG GGCATCCCAG CCCAGGATTC GCAGCCAGAA AACACTTCAG CAGAGCTGTC CAGGAGGCGG GTCAAACACC AGGGAGACCT GTCATCTGGT TACATGGATG ATGTCAAGCC GGAATCAGTG AAAGGCCCCA CCTACTCGAT GTCTCCTAAC AGGACCATGA ACAATGTGGC TTCCTGCAAC CGACTGTTGA ACTTACCGTC AGGGCCCAGG TCTGACTGCC AGCGTAGCCC TCCCGGTGTG CTGAAACTCA ACAACTCTGA TAGCCAGGAC ATCCGTGGGC AGATGCGGGG CAGCCAGAAT GGGCCCATAG CATCCGGGCA CGCTCCAGTG ATCCACTCTA TTCAAAATCT CTATCCAGAA AACTTTGCGC CCTCTTTTCC TCATGGATCC CCTGGGAGGT ACGGAGCTGG TGGGAGGAGT GAAGGTGGTC GGAGCTCTCG GGTCAGCCCT GCAGCTGCTG GCTACCACAG CAGAGCAGCA AAGGCCAAGC CTCCAAAGCA GCAGGGAGCA GGCGATGCGG AGGAGGAAGA GGAGGGATCC GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT GACAAGTAGA GACCACCTCC CCTGCGAGCT AAGCTGGACA GCCAATGACG GGTAAGAGAG TGACATTTTT CACTAACCTA AGACAGGAGG GCCGTCAGAG CTACTGCCTA ATCCAAAGAC GGGTAAAAGT GATAAAAATG TATCACTCCA ACCTAAGACA GGCGCAGCTT CCGAGGGATT TGAGATCCAG ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCAAAAC TAGAATGCAG TGAAAAAAAT GCCTTATTTG TGAAATTTGT GATGCTATTG CCTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA GTCGATAAAC TGGATCTCTG CTGTCCCTGT AATAAACCCG AAAATTTTGA ATTTTTGTAA TTTGTTTTTG TAGTTCTTTA GTTTGTATGT CTGTTGCTAT TATGTCTACT ATTCTTTCCC CTGCACTGTA CCCCCCAATC CCCCCTTTTC TTTTAAAGGC GATACCGTCG AGATCCGTTC ACTAATCGAA TGGATCTGTC TCTGTCTCTC TCTCCACCTT CTTCTTCTAT TCCTTCGGGC CTGTCGGGTC CCCTCGGGGT TGGGAGGTGG GTCTGAAACG ATAATGGTGA ATATCCCTGC CTAACTCTAT TCACTATAGA AAGTACAGCA AAAACTATTC TTAAACCTAC CAAGCCTCCT ACTATCATTA TGAATAATTT TATATACCAC AGCCAATTTG TTATGTTAAA CCAATTCCAC AAACTTGCCC ATTTATCTAA TTCCAATAAT TCTTGTTCAT TCTTTTCTTG CTGGTTTTGC GATTCTTCAA TTAAGGAGTG TATTAAGCTT GTGTAATTGT TAATTTCTCT GTCCCACTCC ATCCAGGTCG TGTGATTCCA AATCTGTTCC AGAGATTTAT TACTCCAACT AGCATTCCAA GGCACAGCAG TGGTGCAAAT GAGTTTTCCA GAGCAACCCC AAATCCCCAG GAGCTGTTGA TCCTTTAGGT ATCTTTCCAC AGCCAGGATT CTTGCCTGGA GCTGCTTGAT GCCCCAGACT GTGAGTTGCA ACAGATGCTG TTGCGCCTCA ATAGCCCTCA GCAAATTGTT CTGCTGCTGC ACTATACCAG ACAATAATTG TCTGGCCTGT ACCGTCAGCG TCATTGACGC TGCGCCCATA GTGCTTCCTG CTGCTCCCAA GAACCCAAGG AACAAAGCTC CTATTCCCAC TGCTCTTTTT TCTCTCTGCA CCACTCTTCT CTTTGCCTTG GTGGGTGCTA CTCCTAATGG TTCAATTTTT ACTACTTTAT ATTTATATAA TTCACTTCTC CAATTGTCCC TCATATCTCC TCCTCCAGGT CTGAAGATCA GCGGCCGGCC GCTTGCTGTG CGGTGGTCTT ACTTTTGTTT TGCTCTTCCT CTATCTTGTC TAAAGCTTCC TTGGTGTCTT TTATCTCTAT CCTTTGATGC ACACAATAGA GGGTTGCTAC TGTATTATAT AATGATCTAA GTTCTTCTGA TCCTGTCTGA AGGGATGGTT GTAGCTGTCC CAGTATTTGT CTACAGCCTT CTGATGTTTC TAACAGGCCA GGATTAACTG CGAATCGTTC TAGCTCCCTG CTTGCCCATA CTATATGTTT TAATTTATAT TTTTTCTTTC CCCCTGGCCT TAACCGAATT TTTTCCCATC GCGATCTAAT TCTCCCCCGC TTAATACTGA CGCTCTCGCA CCCATCTCTC TCCTTCTAGC CTCCGCTAGT CAAAATTTTT GGCGTACTCA CCAGTCGCCG CCCCTCGCCT CTTGCCGTGC GCGCTTCAGC AAGCCGAGTC CTGCGTCGAG AGAGCTCCTC TGGTTTCCCT TTCGCTTTCA AGTCCCTGTT CGGGCGCCAC TGCTAGAGAT TTTCCACACT GACTAAAAGG GTCTGAGGGA TCTCTAGTTA CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA CTCAAGGCAA GCTTTATTGA GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC AGGCTCAGAT CTGGTCTAAC CAGAGAGACC CAGTACAGGC AAAAAGCAGC TGCTTATATG CAGGATCTGA GGGCTCGCCA CTCCCCAGTC CCGCCCAGGC CACGCCTCCC TGGAAAGTCC CCAGCGGAAA GTCCCTTGTA GCAAGCTCGA TATCAGCAGT TCTTGAAGTA CTCCGGATGC AGCTCTCGGG CCACGTGATG AAATGCTAGG CGGCTGTCAA ACCTCCACTC TAACACTTCT CTCTCCGGGT CATCCATCCC ATGCAGGCTC ACAGGGTGTA ACAAGCTGGT GTTCTCTCCT TTATTGGCCT CTTCTACCTT ATCTGGCTCA ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT ATCTGACCCC TGGCCCTGGT GTGTAGTTCT GCTAATCAGG GAAGTAGCCT TGTGTGTGGT AGATCCACAG ATCAAGGATA TCTTGTCTTC TTTGGGAGTG AATTAGCCCT TCCA
