NM_145414.2;9830109N13Rik; Nol1r; Wbscr20; Wbscr20a
100μg liquid plasmid(Instantly):$91
| name | pT3-EF1a-Nsun5(mouse)-myr-AKT-HA | another name | apT3-EF1a-Nsun5(mouse)-myr-AKT-HA |
| Insert Size (bp) | 1395bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | EF1a | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pT3-myr-AKT-HA(mouse) | 5′ sequencing primer | PEF-F |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGCTCCGGTG CCCGTCAGTG GGCAGAGCGC ACATCGCCCA CAGTCCCCGA GAAGTTGGGG GGAGGGGTCG GCAATTGAAC CGGTGCCTAG AGAAGGTGGC GCGGGGTAAA CTGGGAAAGT GATGTCGTGT ACTGGCTCCG CCTTTTTCCC GAGGGTGGGG GAGAACCGTA TATAAGTGCA GTAGTCGCCG TGAACGTTCT TTTTCGCAAC GGGTTTGCCG CCAGAACACA GGTAAGTGCC GTGTGTGGTT CCCGCGGGCC TGGCCTCTTT ACGGGTTATG GCCCTTGCGT GCCTTGAATT ACTTCCACCT GGCTGCAGTA CGTGATTCTT GATCCCGAGC TTCGGGTTGG AAGTGGGTGG GAGAGTTCGA GGCCTTGCGC TTAAGGAGCC CCTTCGCCTC GTGCTTGAGT TGAGGCCTGG CCTGGGCGCT GGGGCCGCCG CGTGCGAATC TGGTGGCACC TTCGCGCCTG TCTCGCTGCT TTCGATAAGT CTCTAGCCAT TTAAAATTTT TGATGACCTG CTGCGACGCT TTTTTTCTGG CAAGATAGTC TTGTAAATGC GGGCCAAGAT CTGCACACTG GTATTTCGGT TTTTGGGGCC GCGGGCGGCG ACGGGGCCCG TGCGTCCCAG CGCACATGTT CGGCGAGGCG GGGCCTGCGA GCGCGGCCAC CGAGAATCGG ACGGGGGTAG TCTCAAGCTG GCCGGCCTGC TCTGGTGCCT GGCCTCGCGC CGCCGTGTAT CGCCCCGCCC TGGGCGGCAA GGCTGGCCCG GTCGGCACCA GTTGCGTGAG CGGAAAGATG GCCGCTTCCC GGCCCTGCTG CAGGGAGCTC AAAATGGAGG ACGCGGCGCT CGGGAGAGCG GGCGGGTGAG TCACCCACAC AAAGGAAAAG GGCCTTTCCG TCCTCAGCCG TCGCTTCATG TGACTCCACG GAGTACCGGG CGCCGTCCAG GCACCTCGAT TAGTTCTCGA GCTTTTGGAG TACGTCGTCT TTAGGTTGGG GGGAGGGGTT TTATGCGATG GAGTTTCCCC ACACTGAGTG GGTGGAGACT GAAGTTAGGC CAGCTTGGCA CTTGATGTAA TTCTCCTTGG AATTTGCCCT TTTTGAGTTT GGATCTTGGT TCATTCTCAA GCCTCAGACA GTGGTTCAAA GTTTTTTTCT TCCATTTCAG GTGTCGTGAG GAATTAGCTT GGTACTAATA CGACTCACTA TAGGGAGACC CAAGCTGGCT AGGTAAGCTT GGTACCGAGC TCGGATCCAC TAGTCCAGTG TGGTGGAATT CTGCAGATAT CAACAAGTTT GTACAAAAAA GCAGGCTCCG CGGCCGCCCC CTTCACCATG GGCCACCATG GGGCTGTACG CGGCCGCCGC GGCGGTGCTG GCCGGCGTGG AAAGCCGCCA GGGCTCCCTC AAGGGGTTGG TGTATTCCAG CAACTTCCAG AACCTGAAGC AGTTGTACGC TCTGGTGTGC GAGACGCAGC GCTACTCGGC CGTGCTGGAC GCTGTCATCG CCAGTGCTGG GCTCCTTCGC GCCGAGAAGA AGCTGAGACC GCACCTTGCC AAGGTGCTAG TGTATGAATT ATTGCTGGGG AAGGGGTTTC GAGGGGGTGG AGGCCGATGG AAGGCTCTGC TGGGCCGGCA CCAGGCAAGG CTCAAGGCTG AGCTGGCCCG ACTCAAGGTT CATCGGGGTG TGAGCCGAAA TGAGGACCTG TTGCAAGAGA GCTCCAGACC TGGCCAAGCC TACCAGGTAC CACGGTTCGT GCGTGTGAAC ACGCTCAAGA CCCGCCCTGA GGATGCAATC GATTATTTCA AGAGACAAGG TTTCTCCTAC CAGGGTCGGG CTTCCAGCCT GGAAGACTTG CGAGCTCTCA AGGGGCAGCA TTTTCTTTTG GATCCCTTGC TGCCTGAGCT TCTTGTATTT CCTGCACAGA CAGATCTGCA CGAGCATCCA CTCTACCGTG CTGGCCATCT CATCCTGCAG GACAAGGCCA GCTGCCTCCC AGCCATGCTG CTGTCCCCCC CGCCAGGCTC CCATGTCATT GATGCCTGTG CTGCCCCTGG TAACAAGACC AGCTACATTG CAGCCCTTCT AAAGAACCAG GGGAAGATCT TTGCTTTCGA CCAGGACGCT AAGCGGCTGG CAGCCATGGC GACGTTGGTG GCTCGGGCTG GGGTCTCTTG CTGCGAGTTA GCAGAGAAGG ACTTCCTGAC AGTCTCTCCC TCTGACCAGC GTTACAGCCA GGTCCAGTAC ATCTTGCTGG ATCCTTCTTG TAGTGGCTCT GGCATGCTGA GCCGGCAGCT GGAGGAACAT GGGGAAGGCA CACCTAGCAA GGAGCGCTTG CAGGCCTTGG CCGGGTTCCA GCAGCGGGCT CTGTGCCATG CACTTAGGTT CCCCTCTCTG CAGCGCCTAG TCTACTCCAC GTGTTCCCTT TGCCAAGAAG AGAATGAAGA CGTGGTCCAG GAGGCTCTAC AGCACAACTC GGGGACATTC AGGTTGGCTC CTGTCCTACC CACATGGCCT CACCGAGGTC TGAGTACCTT TCCAGGGTCT GAACACTGCC TCCGGGCTTC CCCTGAGACC ACGCTCACTG GTGGCTTCTT CATTGCTGTG TTTGAACGTG CAGAGGTGGT ACCAACCCCA GCCCCACAGA CCGATGCAAT GGATCCAGAA CCTCTCAGCC AAGTCCCAAA GAGAAAGAGA AGACGCAAAG CAGCAGTTGG TGCCAGCATG CAGCCAAGCA CAGGCGGCGG AGGGAGTGGA GGAGGGGGAA GCATGGGGAG CAGCAAGAGC AAGCCCAAGT CTAGAATGAA CGACGTAGCC ATTGTGAAGG AGGGCTGGCT GCACAAACGA GGGGAATATA TTAAAACCTG GCGGCCACGC TACTTCCTCC TCAAGAACGA TGGCACCTTT ATTGGCTACA AGGAACGGCC TCAGGATGTG GATCAGCGAG AGTCCCCACT CAACAACTTC TCAGTGGCAC AATGCCAGCT GATGAAGACA GAGCGGCCAA GGCCCAACAC CTTTATCATC CGCTGCCTGC AGTGGACCAC AGTCATTGAG CGCACCTTCC ATGTGGAAAC GCCTGAGGAG CGGGAAGAAT GGGCCACCGC CATTCAGACT GTGGCCGATG GACTCAAGAG GCAGGAAGAA GAGACGATGG ACTTCCGATC AGGCTCACCC AGTGACAACT CAGGGGCTGA AGAGATGGAG GTGTCCCTGG CCAAGCCCAA GCACCGTGTG ACCATGAACG AGTTTGAGTA CCTGAAACTA CTGGGCAAGG GCACCTTTGG GAAAGTGATT CTGGTGAAAG AGAAGGCCAC AGGCCGCTAC TATGCCATGA AGATCCTCAA GAAGGAGGTC ATCGTCGCCA AGGATGAGGT TGCCCACACG CTTACTGAGA ACCGTGTCCT GCAGAACTCT AGGCATCCCT TCCTTACGGC CCTCAAGTAC TCATTCCAGA CCCACGACCG CCTCTGCTTT GTCATGGAGT ATGCCAACGG GGGCGAGCTC TTCTTCCACC TGTCTCGAGA GCGCGTGTTC TCCGAGGACC GGGCCCGCTT CTATGGTGCG GAGATTGTGT CTGCCCTGGA CTACTTGCAC TCCGAGAAGA ACGTGGTGTA CCGGGACCTG AAGCTGGAGA ACCTCATGCT GGACAAGGAC GGGCACATCA AGATAACGGA CTTCGGGCTG TGCAAGGAGG GGATCAAGGA TGGTGCCACT ATGAAGACAT TCTGCGGAAC GCCGGAGTAC CTGGCCCCTG AGGTGCTGGA GGACAACGAC TACGGCCGTG CAGTGGACTG GTGGGGGCTG GGCGTGGTCA TGTATGAGAT GATGTGTGGC CGCCTGCCCT TCTACAACCA GGACCACGAG AAGCTGTTCG AGCTGATCCT CATGGAGGAG ATCCGCTTCC CGCGCACACT CGGCCCTGAG GCCAAGTCCC TGCTCTCCGG GCTGCTCAAG AAGGACCCTA CACAGAGGCT CGGTGGGGGC TCTGAGGATG CCAAGGAGAT CATGCAGCAC CGGTTCTTTG CCAACATCGT GTGGCAGGAT GTGTATGAGA AGAAGCTGAG CCCACCTTTC AAGCCCCAGG TCACCTCTGA GACTGACACC AGGTATTTCG ATGAGGAGTT CACAGCTCAG ATGATCACCA TCACGCCGCC TGATCAAGAT GACAGCATGG AGTGTGTGGA CAGTGAGCGG AGGCCGCACT TCCCCCAGTT CTCCTACTCA GCCAGTGGCA CAGCCTACCC ATACGACGTC CCAGACTACG CTTGAAAGGG TGGGCGCGCC GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCCAGCACA GTGGCGGCCG CTCGAGTCTA GAGGGCCCGC GGTTCGAAGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG CGTACCGGTT AGTAATGAGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AAAGAACCAG CTGGGGCTCT AGGGGGTATC CCCACGCGCC CTGTAGCGGC GCATTAAATA ACTTCGTATA ATGTATGCTA TACGAAGTTA TATCGTATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GAATTCCTTG AAATACATCC ACAGGTACAC CTCCAATTGA CTCAAATGAT GTCAATTAGT CTATCAGAAG CTTCTAAAGC CATGACATCA TTTTCTGGAA TTTTCCAAGC TGTTTAAAGG CACAGTCAAC TTAGTGTATG TAAACTTCTG ACCCACTGGA ATTGTGATAC AGTGAATTAT AAGTGAAATA ATCTGTCTGT AAACAATTGT TGGAAAAATG ACTTGTGTCA TGCACAAAGT AGATGTCCTA ACTGACTTGC CAAAACTATT GTTTGTTAAC AAGAAATTTG TGGAGTAGTT GAAAAACGAG TTTTAATGAC TCCAACTTAA GTGTATGTAA ACTTCCGACT TCAACTGTAG GGTACCGAGC TCGAATTCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGACCT ATACAGTTGA AGTCGGAAGT TTACATACAC TTAAGTTGGA GTCATTAAAA CTCGTTTTTC AACTACTCCA CAAATTTCTT GTTAACAAAC AATAGTTTTG GCAAGTCAGT TAGGACATCT ACTTTGTGCA TGACACAAGT CATTTTTCCA ACAATTGTTT ACAGACAGAT TATTTCACTT ATAATTCACT GTATCACAAT TCCAGTGGGT CAGAAGTTTA CATACACTAA GTTGACTGTG CCTTTAAACA GCTTGGAAAA TTCCAGAAAA TGATGTCATG GCTTTAGAAG CTTCTGATAG ACTAATTGAC ATCATTTGAG TCAATTGGAG GTGTACCTGT GGATGTATTT CAAGGGGATC CAGACATGAT AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG CAGCGGCCGC TAGCTCGAGA TCTTAATTAA ACTAGTATCG ATATAACTTC GTATAATGTA TGCTATACGA AGTTATATGT ACGGGCCAGA TATACGCGTT GACATTGATT ATTGACTAGG CTTTTGCAAA AAGCTTTGCA AAGATGGATA AAGTTTTAAA CAGAGAGGAA TCTTTGCAGC TAATGGACCT TCTAGGTCTT GAAAGGAGTG GGAATT
