pBiFC-CMV-FLAG-HDAC6(pig)-VN173-Puro
$99.00
In stock
SKU
PL22985
XM_005673600.3;
100μg liquid plasmid(Instantly):$120
| name | pBiFC-CMV-FLAG-HDAC6(pig)-VN173-Puro | another name | apBiFC-CMV-FLAG-HDAC6(pig)-VN173-Puro |
| Insert Size (bp) | 3477bp | Plasmid host | Mammary cell |
| Plasmid uses | Bimolecular fluorescence | Fragment type | BIFC |
| Fragment species | Pig | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pBiFC-VN173-Puro | 5′ sequencing primer | CMV-F |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CCACCATGGA CTACAAAGAC GATGACGACA AGCTTATGGA AAAGAGAGGC TCAGGCACCC GCGGCCCTAC AGGTCGCCGA GAAATTGGCG GCGCGCGCCA AGGGCGGAGT TTGAGAAAGG GGCTGCGCCA AAACCGCGTC AGGGGTGAAG TCTCCCCAGC CATGACCTCC ACCGGCCAGG ATTCCACCAC AACCAGGCAG CGAAGAAGTA GGCACAACCC CCACTCCCCC CCACACGACT CCAGCGTCAC CTCGAAGCGA GGTGTTAAAA AGGGTGCCGG ACTCCGCTCC AGCCCCAATC TAGCGGATGT AAAGAAGAAA GGCAGAATGA AGAAGCTTAG CCAAGCAGCC GAGCAAGACC TAATCGTGGG GCTCCAAGGG CTGGATCTGA ACCTGGAGGC CAGGACACCG TCTGGCACTG GCTTGGTGTT GGATGAGCAG CTAAATGAAT TCCACTGCCT CTGGGATGAC AGCTTCCCTG AAGGCCCTGA GCGGCTCCAT GCCATCAAGG AGCAGCTGAT GCAGGAGGGC CTCCTGGACC GCTGCGTGTC CTTTCAGGCC CGATTCGCCG AAAAGGAGGA GCTTATGTTG GTTCACAGCC TAGAGTACAT TGATCTGATG GAGACGACCC AGTACATGAA TGAGGGGGAG CTCCGCGTCC TAGCAGACAC CTATGATTCG GTTTATCTGC ATCCGAACTC ATACACCTGT GCCTGCCTGG CCTCAGGCTC CGTCCTCAGG CTGGTGGATG CCGTCCTGGG GGCTGAGATC CGGAATGGCA TGGCCATCAT CAGGCCTCCT GGACACCACG CCCAGCACAG TCTTATGGAC GGGTATTGCA TGTTCAACCA CGTAGCCTTG GCCGCCCGCT ACGCTCAGCA GAAGCATGAC GTTCAGAGGG TCCTTATTGT GGATTGGGAT GTGCACCATG GTCAGGGAAC ACAGTTCACC TTTGACCAAG ACCCCAGCGT CCTCTATTTC TCCATCCACC GCTATGAGCA GGGTCGGTTC TGGCCCCACC TTAAGGCCTC TAATTGGTCC ACCACGGGTT TTGGCCAAGG CCAGGGATAC ACCATCAATG TGCCTTGGAA CCAGGTGGGG ATGCGAGATG CCGACTACAT TGCTGCTTTC CTGCACCTCC TGCTGCCAGT CGCCCTTGAG TTCCAGCCCC AGCTGGTCCT GGTAGCCGCT GGCTTTGATG CCCTCCAAGG GGACCCCAAG GGTGAGATGG CCGCCACTCC GGCAGGGTTC GCCCAGCTAA CTCATCTGCT CATGGGTCTG GCAGGAGGCA AGTTGATCCT CTCACTGGAG GGCGGCTACA ACCTCCGCTC CCTGGCTGAG GGCGTCAGTG CCTCACTCCA CACCCTTCTG GGAGACCCTT GTCCCTTGCT GGAGTTCCCG GGCGCCCCCT GTCCCAGTGC CCAGGCGTCA CTATCCTGTG CTCTGGAAGC CCTTGAGCCC TTCTGGGAGG TCCTTGTGAG ATCTGCTGAG ACCCTGGAGG ACGAGGACAC CGTGGAGGGG GACCATGTGG AGGAGGAGGA GGAACCATGG CAGCCCCCTG AGCTACCGAT GCTGGCGTGG CCGGTGCTGC AGGCTCGCAC AGGGCTGGTC TACGACCAAC AGATGATGGA TCACTACAAC TTATGGGACA GCCACCACCC CGAGATGCCT CAGCGCATCC TGCGTATCAT GCGCCGACTG GAGGAGCTGG GCCTCGCCGG GCGCTGCCTC GCTCTGCCCG CCCGTCCCGC CACGGATGCT GAGCTGCTTG CCTGCCACAG TGCTGAATAC GTGAGTCGCA TCCGGGCCAC GGAGAAGATG AGGACCCGGG AGCTGCACCG CGAGGGTGCC AACTATGACT CCATCTACAT CTGCCCTGGC ACCTTCGCCT GTGCGCAGCT GGCCGCGGGC GCCGCCTGCC GCCTGGTGGA GGCTGTGCTG GCAGGAGAGG TTTTGAATGG TATTGCCGTG GTGCGCCCCC CTGGACACCA CGCAGAAAGG GACTCGGCTT GCGGTTTCTG CTTTTTCAAT TCTGTGGCAG TAGCTGCTCG CCATGCTCAG GCCATCAGTG GGCATGCGCT GCGGATCCTG ATCGTGGACT GGGACGTCCA TCATGGTAAC GGCACTCAGC ACTTATTTGA GGAGGACCCC AGCGTGCTCT ACATTTCCCT GCACCGCTAC GATCATGGCA CCTTCTTTCC CATGGGGGAT GAGGGTGCCA GCAGCCAGAT AGGTCAGGCT GCAGGCACGG GCTTCACTGT CAACGTGGCC TGGAATGGGC CCCGCGTGGG TGACCCCGAG TACCTGGCTG CATGGCATCG GCTGGTGCTT CCTATTGCCT ATGAGTTTAA CCCGGAACTG GTGCTGGTCT CAGCTGGTTT CGACGCTGCC CGGGGGGACC CGCTGGGTGG CTGCCAGGTG TCACCTGAGT GCTACGCCCA CCTCACCCAC CTGCTGATGG GCCTGGCCAA TGGCCGTATT ATTCTAATCC TAGAGGGTGG CTATAACCTG ATATCCATCT CGGAGTCCAT GGCTGCCTGT ACCCGCTCCC TCCTTGGGGA CTCACCACCC CTGCTGACCC TGCTGAGGCC CCCACTCTCA CGGGCCCTGG CCTCGATCGC CGAAACCACC CAAATCCATC GCAGATACTG GCGCAGCTTG CGGGTCACAA AGGTCGAAGA CAAAGAGAGA CCCTCCAGTT CTAAGTCGTT CGCCAAGAAG CCATCCCAAC CAGCCAAGCC TAAGCCATCC AAGGGGATGA CCGCCCCAAA AGGGGACATT CCGGAAGCAC ACACAGGGAA GGCCACCTCG GTATCATCAG TGAAAGTGTC CCCCCCAGGC CAGACGAAGT CAGAGGCAGC CCTAGTGGAA CTTACTCAGG ACCAGTCCTC AAAGGCTGCC ACAGAGGAGG CTGTGGGAGG AGCTAAGCTG ATCCAAAGCC CTCCAGCCTC GGGAGGTGAC AACCAGACTC CTCCAGCTTC TCCACGGCAA GGATCCACAA CTCTGATCTC CCCCAGCAAG CTGGTAGGCA ATCTCGGAGC CTTGGAACTG AGAAATGAGG TTCAGGAGTC CCCAAAAGAG GAAGGGCTGC TAGGAGAGGC AGCTGGAGGT CAGAACATGG ACAATTTAAT GCTGATGCAG GTCTTTGGAA ACCAAGCTGA CACTGACCAG GCCATGTTCT ATGCTGTGTC ACCACTGCTC TGGTGTCCAC ATGTGGTGGC AGTACGCCCC GTACCTGAAG CGGGCCTGGA CGTGACCCAA CCTTGTCAAG ACTGTGGAGC ACTCCAGGAG AACTGGGTGT GTCTGTCTTG CTATCAGGTC TGCTGCGGCC GTTATATCAA CGCCCATATG CTCCAGCACC ACGAAAGCTC AGGCCACCCC ATGGTCCTCA GCTACGCCGA CCTGTCTACC TGGTGTTACC CCTGCCAGGC CTATGTTCAC CACGAGGCTC TCCTGGCTGT GAAGAACATC GCCCACCAGA ACAAGTTTGG GGAGGGCGTG CCCCACTCCC GCTCTAGAAG ATCCATCGCC ACCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA GCTGATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGGG CTACGGCCTG CAGTGCTTCG CCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA CAACAGCCAC AACGTCTATA TCACCGCCGA CAAGCAGAAG AACGGCATCA AGGCCAACTT CAAGATCCGC CACAACATCG AGTAGAGAGG GCCCGTTTAA ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG AACCAGCTGG GGCTCTAGGG GGTATCCCCA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTAA TTCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC TGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ACCGAGTACA AGCCCACGGT GCGCCTCGCC ACCCGCGACG ACGTCCCCCG GGCCGTACGC ACCCTCGCCG CCGCGTTCGC CGACTACCCC GCCACGCGCC ACACCGTCGA CCCGGACCGC CACATCGAGC GGGTCACCGA GCTGCAAGAA CTCTTCCTCA CGCGCGTCGG GCTCGACATC GGCAAGGTGT GGGTCGCGGA CGACGGCGCC GCGGTGGCGG TCTGGACCAC GCCGGAGAGC GTCGAAGCGG GGGCGGTGTT CGCCGAGATC GGCCCGCGCA TGGCCGAGTT GAGCGGTTCC CGGCTGGCCG CGCAGCAACA GATGGAAGGC CTCCTGGCGC CGCACCGGCC CAAGGAGCCC GCGTGGTTCC TGGCCACCGT CGGCGTCTCG CCCGACCACC AGGGCAAGGG TCTGGGCAGC GCCGTCGTGC TCCCCGGAGT GGAGGCGGCC GAGCGCGCCG GGGTGCCCGC CTTCCTGGAG ACCTCCGCGC CCCGCAACCT CCCCTTCTAC GAGCGGCTCG GCTTCACCGT CACCGCCGAC GTCGAGGTGC CCGAAGGACC GCGCACCTGG TGCATGACCC GCAAGCCCGG TGCCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCGACGG ATCGGGAGAT CTCCCGATCC CCTATGGT
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