pBJHF-EGFP-Fyco1(mouse)-Hyg
$113.00
In stock
SKU
PL22950
NM_148925.3;"2810409M01Rik; Mem2; RUFY3; ZFYVE7
100μg liquid plasmid(Instantly):$134
| name | pBJHF-EGFP-Fyco1(mouse)-Hyg | another name | apBJHF-EGFP-Fyco1(mouse)-Hyg |
| Insert Size (bp) | 4314bp | Plasmid host | Bacteria |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Hyg | Fluorescent labeling | Green |
| Promoter | CAG | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pBJHF-Hygro-GFP-TAA | 5′ sequencing primer | pEGFP-C-5' |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACATTGATT ATTGACTAGT TATTAATAGT AATCAATTAC GGGGTCATTA GTTCATAGCC CATATATGGA GTTCCGCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTCGAGGTGA GCCCCACGTT CTGCTTCACT CTCCCCATCT CCCCCCCCTC CCCACCCCCA ATTTTGTATT TATTTATTTT TTAATTATTT TGTGCAGCGA TGGGGGCGGG GGGGGGGGGG GCGCGCGCCA GGCGGGGCGG GGCGGGGCGA GGGGCGGGGC GGGGCGAGGC GGAAAGGTGC GGCGGCAGCC AATCAGAGCG GCGCGCTCCG AAAGTTTCCT TTTATGGCGA GGCGGCGGCG GCGGCGGCCC TATAAAAAGC GAAGCGCGCG GCGGGCGGGA GTCGCTGCGT TGCCTTCGCC CCGTGCCCCG CTCCGCGCCG CCTCGCGCCG CCCGCCCCGG CTCTGACTGA CCGCGTTACT CCCACAGGTG AGCGGGCGGG ACGGCCCTTC TCCTCCGGGC TGTAATTAGC GCTTGGTTTA ATGACGGCTC GTTTCTTTTC TGTGGCTGCG TGAAAGCCTT AAAGGGCTCC GGGAGGGCCC TTTGTGCGGG GGGGAGCGGC TCGGGGGGTG CGTGCGTGTG TGTGTGCGTG GGGAGCGCCG CGTGCGGCTC CGCGCTGCCC GGCGGCTGTG AGCGCTGCGG GCGCGGCGCG GGGCTTTGTG CGCTCCGCAG TGTGCGCGAG GGGAGCGCGG CCGGGGGCGG TGCCCCGCGG TGCGGGGGGG GCTGCGAGGG GAACAAAGGC TGCGTGCGGG GTGTGTGCGT GGGGGGGTGA GCAGGGGGTG TGGGCGCGGC GGTCGGGCTG TAACCCCCCC CTGCACCCCC CTCCCCGAGT TGCTGAGCAC GGCCCGGCTT CGGGTGCGGG GCTCCGTACG GGGCGTGGCG CGGGGCTCGC CGTGCCGGGC GGGGGGTGGC GGCAGGTGGG GGTGCCGGGC GGGGCGGGGC CGCCTCGGGC CGGGGAGGGC TCGGGGGAGG GGCGCGGCGG CCCCCGGAGC GCCGGCGGCT GTCGAGGCGC GGCGAGCCGC AGCCATTGCC TTTTATGGTA ATCGTGCGAG AGGGCGCAGG GACTTCCTTT GTCCCAAATC TGTGCGGAGC CGAAATCTGG GAGGCGCCGC CGCACCCCCT CTAGCGGGCG CGGGGCGAAG CGGTGCGGCG CCGGCAGGAA GGAAATGGGC GGGGAGGGCC TTCGTGCGTC GCCGCGCCGC CGTCCCCTTC TCCCTCTCCA GCCTCGGGGC TGTCCGCGGG GGGACGGCTG CCTTCGGGGG GGACGGGGCA GGGCGGGGTT CGGCTTCTGG CGTGTGACCG GCGGCTCTAG AGCCTCTGCT AACCATGTTC ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG GCAACGTGCT GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTCCTC GACGGTATCG ATAAGCTATC ACAAGTTTGT ACAGCCACCA TGCCCGGGAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTAT ACAAGGGCGG TGGAGGCAGT GGTGGAGGCG GTAGCGGATC CATGGCTTCT AGCAGCACTG AGACTCAGCT CCAGAGGATC ATCCGTGATC TACAAGATGC AGCGACAGAA CTAAGCCATG AATTTAAGGA AGGCGGAGAG CCAATCACAG ATGACAGCAC CAGTTTGCAC AAGTTTTCTT ACAAACTTGA GTATCTTCTG CAATTTGATC AGAAGGAGAA GGCGAGCCTC CTGGGAAGCA AGAAAGACTA CTGGGATTAC TTTTGTGCGT GCCTAGCCAA GGTGAAAGGA GCAAATGATG GCATTCGATT TGTCAGGTCC ATCTCTGAGC TCCGAACATC TCTGGGGAAA GGGAGAGCCT TCATTCGCTA CTCTTTGGTG CACCAGAGGC TGGCAGACAC CTTGCAGCAG TGCTTCATGA ACACTAAAGT GACCAGTGAC TGGTATTATG CAAGAAGCCC CTTTCTGAAG CCAAAGCTGA GCTCTGACAT TGTGGGTCAA CTGTATGAGC TGACTGAGGT TCAGTTTGAT CTAGCACCAA GAGGCTATGA CTTGGATGCC GCCTGGCCAA CATTTGCCAG GAGGACGCTA GCCACCAGCA CGTCTGCTTA CATGTGGAAA CCCCCCAGCC GAAGCTCCAG CATGAGTAGT TTAGTGAGCA ACTACTTGCA GACTCAGGAG ATGGCCTCCA GTCTTGACCT GAATTGTTCT CTAAACAATG AAGCACTAGA AAGCTTCGAC GAGATGCGGC TGGAGCTGGA CCAGTTGGAG GTTCGGGAGA AACAGCTACA AGAACGTGTA CAGCAGCTAG ACAGGGAGAA CCAAGCACTG CGAATGTTGG TCAGCAGACA AGGGGGGCAG CTTCAGGTAG AGAAGGAGAT GGGGTACCTT GCAGTCGAGG ACAGCATTGG CCTTGTGAGC CTGGTAGCAG AACTCCAGAA GCAGGGGGAC GTCAGCCAGG CCACAGTGAA GAAGCTACAG TCATGTCTGC AGGCCTTGGA GCTAAATGTA GACAAGAAAG AGTACAGTCC CTCAGCCTTG CAGCTTGAGA ACATGGCGAA GGAGCTTGAC ACTGTGAGAG GCTCCTTGGG TAGGGAGAAC CAGCTCCTGG CCAGCCTTTC AGAGCGCCTT GCTAGGGCAG AGAAAGGGGA AAAGACACCT CCAGACACAG AGCTTCATCA GGAGCCAGTT CCTGCGGATC TGGTGCTCAA GTTCCAGGAG CTGAAGGGAA AACTTCAAGC CCTAGAAGGA GAGAACACCG AGGCCCAGGA GCTCAACAGG CAGCAGAGTA TCAAGCTAGA GCAACTGGCC AAGGAACTAC AGCTGAAGGA GGAGGCCCGG GCTAGCCTAG CACACTTAGT GAAGGATGTG GTCCCACTTC AGGAAGAGCT GTCTGGAAAG AAGCAAGAGT CAGCTCAGCT CCGACGACAG CTTCAGGAAT CCCTGGCCCA CTTGAGCTCT GTGGAGGAGG AGCTGGCTGA AGCCAGGCAG CAGGAAAAGC AGCATCGAGA AGAAAAGCAG CTACTGGAGC AGGAAGCCAC GTCTCTTACA TGGCAGCTGC AGCTCCTAGA GACCCAGCTA GGACAGGTGA GTCAGCTTGT AAGTGACCTG GAGGAGCAGA AGAAGCAACT CATGCAGGAG AGAGACCATC TCAGCCAGAG AGTGGGCACA CTGGAGCAGC TAGCCGAGGT GCATGGCCCG CCACAGTCTG CGGAGATGCC GGAAAAGAGG CAGCAGTGCC TCCGGGAAGA ACAGGTAAAC AATAGCACAG TGTCAGAGGC AGAGCAGGAG GAGTTGCAGA AGGAGCTGCA GAACATGGTT GACCGCAACC AGCTCCTCGA AGGCAAACTG CAGGCCTTGC AAACTGACTA TAAGGCACTG CAGCAGCGAG AGGCAGCAAT CCAGGGCTCT TTAGCCTCCC TGGAAGCTGA ACAGGCTAGC ATCCGGCACC TGGGAAACCA GATGGAAGCA AGCCTCCTGG CTGTGAAGAA AGCCAAAGAG ACTATGAAAG CCCAGGTGGC AGAGAAGGAG GCTGCCCTGC AGAGTAAGGA GAGTGAGTGC CAGAGGCTGC AGGAAGAGGC AGATCAGTGC CGGCTTCAGG CAGAGGCCCA GGCCCAGGAG CTCAGAGCTC TTGAGAACCA GTGCCAGCAG CAGATTCAGC TGATTGAGGT CCTCTCTGCA GAGAAAGGCC AACAGGGACT CAGCCTGCCC CAAGTCAACA CAGACCAACT GGCCCTGTCT CAAGCACAGC TGGAAATCCA CCAGGGGGAA GCCCAGCGGT TACAGAATGA GGTGGTGGAC CTCCAGGCCA AGCTCCAGGT GGCCCTAGGT GATCGAGACA AGTTACAGAG CCAGCTGGGT GTGGCTGAGA CAGTTCTAAG GGAGCACAAG ACCCTGGTGC AACAACTGAA AGAGCAGAAT GAAGCCCTCA ACAGGGCCCA TGTTCAGGAG CTGCTTCAGT GCTCAGAGAG AGAGGGGATA CTACAGGAGG AGAGCATCTA CAAGGCCCAG AAGCAGGAAC AAGAGCTACG AGCCCTGCAG GCAGAGCTGT CACAGGTGAG GTGCAGCTCT GAGGGAGCCC ACCTGGAACA TGCAGAGCTG CAAGACCAGC TGCACCGAGC CAACACAGAC ACAGCTGAGC TTGGTATACA GGTCTGTGCA CTGACAGCTG AGAAGGATCG AATGGAGGAG GCCCTGGCCA GCCTAGCCCA GGAGCTCCAG GACTCCAAAG AGGCAGCACT ACAGGAACGA AAGGGCTTGG AGCTCCAAGT GATGCAACTT CAGCAAGAGA AGGAGAAGTT GCAGGAAAAG GTGAAGGCAG CTGAGGAGGC AGCCAGTTCA TTCTCTGGTC TGCAGGCACA GCTGGCCCAG GCTGAGCAGC TAGCCCAGAG CCTCCAAGAG ACTGCACACC AGGAACAAGA TGCCCTCAAG TTCCAACTAA GTGCTGAGAT TATGGACCAC CAGAACCGAT TAAAGACAGC CAATGAAGAG TGTGGGCACC TCAGGGCCCA GCTAGAAGAA CAAGGCCAGC AGCTGCAAAT GACTAAGGAG GCTGTGCAGG AACTGGAGAT CACCAAGGCG GCCATGGAGG AGAAGCTGAA TTGCACCAGT AGCCACCTTG CAGAGTGCCA GGCCACTTTA CTGCGCAAAG ATGAAGAAAG CACTATGCTT CAAACCAGTC TAGAAAGAAC ACAGAAGGAA CTTGAAAAGG CTACATCAAA AATTCAAGAA TATTACAACA AACTCTGCCA AGAGGTGACA AACAGGGAAA GGAATGACCA GAAGATGCTT GCAGACCTCG ATGACCTGAA CAGAACCAAG AAATACCTTG AGGAGCGGCT GATAGAGCTG CTCAGGGACA AAGATGCTCT CTGGCAAAAA TCCGATGCAC TGGAATTCCA GCAGAAGCTC AGTGCTGAAG AGAAATGTCT TGGGGACATG GAAGTCAACC ACTGCCATGA CTGCAAGCGG GAGTTCAGCT GGATAGTGCG ACGGCACCAC TGCAGGATAT GTGGCCGTAT CTTCTGTTAC TACTGCTGTA ATAACTATGT TGTGACTAAG CCCAGTGGCA AGAAGGAACG CTGCTGCCGA GCCTGCTTCC AGAAGTTCGG CGAAGGCTCT GGATCCAATG ATAGCAGTGG TTCAGGCACT AGCCAGGGAG AGCCCAGCCC CATGGTGTCA CCAGCTGAAG CAAGTCCCCA GTCCATAGGA AGCCAAGGGA TAAACTCAGT CTGCAGACCA CCAGACGATG CTGTGTTTGA CATCATCACT GATGAAGAAC TGTGCCAGAT CCAAGAATCT GGCTCCTCCT TGCCTGAAAC ACCCACTGAA ACTGATTCAA TGGACCCGAA TACGGCTGAA CAGGACACCA CATCAAACTC ATTAACCCCT GAAGACACTG AAGACGTGCC CATGGGGCAA GATGCTGAAA TCTGCTTGCT GAAGTCAGGA GAGCTGATGA TCAAATTACC CCTCACAGTA GAGGAGGTCG CCAGCTTCGG GGAGGGCAGC AGGGAGCTGT TTGTGAGGTC CAGTACCTAC AGCCTGATCA CCATCACCGT GGCTGAGCCT GGCCTCACCA TCAGCTGGGT CTTCTCTTCT GATCCCAAGA GCATCTCCTT CAGTGTGGTC TTCCAAGAGA CAGAAGACAC GCCGCTCGAC CAGTGCAAGG TCCTCATTCC CACCACCCGA TGCAATTCCC ACAAGGAGAA CATCCGGGGC CAGCTGAAGG TCCGAATCCC TGGCATCTAC TTGCTCATCT TTGACAACAC CTTTTCAAGA TTTATCTCCA AAAAAGTGCT TTACCACCTG ACTGTTGACC GGCCTGTGAT CTACGATGGA AGCGATTTCC CATAGGCTAG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG GCTTCTGAGG CGGTTAATTA AAATAAAACT AGAGCGGGCC CGGTACTAAC CAAACTGGAT CTCTGCTGTC CCTGTAATAA ACCCGAAAAT TTTGAATTTT TGTAATTTGT TTTTGTAATT CTTTAGTTTG TATGTCTGTT GCTATTATGT CTACTATTCT TTCCCCTGCA CTGTACCCCC CAATCCCCCC TTTTCTTTTA AAATTGTGGA TGAATACTGC CATTTGTCTG CAGAGCGGCC GCGTCGAGAT AACTTCGTAT AATGTATGCT ATACGAAGTT ATGTTTTGTT ACTTTATAGA AGAAATTTTG AGTTTTTGTT TTTTTTTAAT AAATAAATAA ACATAAATAA ATTGTTTGTT GAATTTATTA TTAGTATGTA AGTGTAAATA TAATAAAACT TAATATCTAT TCAAATTAAT AAATAAACCT CGATATACAG ACCGATAAAA CACATGCGTC AATTTTACGC ATGATTATCT TTAACGTACG TCACAATATG ATTATCTTTC TAGGGTTAAA GAGCTCCAGC TTTTGTTCCC TTTAGTGAGG GTTAATTCGC GCGCTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG CTTACAATTT AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA CGCCAAGCGC GCAATTAACC CTCACTAAAG GGAACAAAAG CTGGGTACCT TAACCCTAGA AAGATAGTCT GCGTAAAATT GACGCATGCA TTCTTGAAAT ATTGCTCTCT CTTTCTAAAT AGCGCGAATC CGTCGCTGTG CATTTAGGAC ATCTCAGTCG CCGCTTGGAG CTCCCGTGAG GCGTGCTTGT CAATGCGGTA AGTGTCACTG ATTTTGAACT ATAACGACCG CGTGAGTCAA AATGACGCAT GATTATCTTT TACGTGACTT TTAAGATTTA ACTCATACGA TAATTATATT GTTATTTCAT GTTCTACTTA CGTGATAACT TATTATATAT ATATTTTCTT GTTATAGATA TCCAACTATG AAACATTATC ATAAAGCAAT AGTAAAGAGC CTTCAGTAAA GAGCAGGCAT TTATCTAATC CCACCCCACC CCCACCCCCG TAGCTCCAAT CCTTCCATTC AAAATGTAGG TACTCTGTTC TCACCCTTCT TAACAAAGTA TGACAGGAAA AAACTTCCAT GTGGACATCT TTATTGTTTA ATAGATCATC AATTTCTCGA CTATTCCTTT GCCCTCGGAC GAGTGCTGGG GCGTCGGTTT CCACTATCGG CGAGTACTTC TACACAGCCA TCGGTCCAGA CGGCCGCGCT TCTGCGGGCG ATTTGTGTAC GCCCGACAGT CCCGGCTCCG GATCGGACGA TTGCGTCGCA TCGACCCTGC GCCCAAGCTG CATCATCGAA ATTGCCGTCA ACCAAGCTCT GATAGAGTTG GTCAAGACCA ATGCGGAGCA TATACGCCCG GAGCCGCGGC GATCCTGCAA GCTCCGGATG CCTCCGCTCG AAGTAGCGCG TCTGCTGCTC CATACAAGCC AACCACGGCC TCCAGAAGAA GATGTTGGCG ACCTCGTATT GGGAATCCCC GAACATCGCC TCGCTCCAGT CAATGACCGC TGTTATGCGG CCATTGTCCG TCAGGACATT GTTGGAGCCG AAATCCGCGT GCACGAGGTG CCGGACTTCG GGGCAGTCCT CGGCCCAAAG CATCAGCTCA TCGAGAGCCT GCGCGACGGA CGCACTGACG GTGTCGTCCA TCACAGTTTG CCAGTGATAC ACATGGGGAT CAGCAATCGC GCATATGAAA TCACGCCATG TAGTGTATTG ACCGATTCCT TGCGGTCCGA ATGGGCCGAA CCCGCTCGTC TGGCTAAGAT CGGCCGCAGC GATCGCATCC ATGGCCTCCG CGACCGGCTG CAGAACAGCG GGCAGTTCGG TTTCAGGCAG GTCTTGCAAC GTGACACCCT GTGCACGGCG GGAGATGCAA TAGGTCAGGC TCTCGCTGAA TTCCCCAATG TCAAGCACTT CCGGAATCGG GAGCGCGGCC GATGCAAAGT GCCGATAAAC ATAACGATCT TTGTAGAAAC CATCGGCGCA GCTATTTACC CGCAGGACAT ATCCACGCCC TCCTACATCG AAGCTGAAAG CACGAGATTC TTCGCCCTCC GAGAGCTGCA TCAGGTCGGA GACGCTGTCG AACTTTTCGA TCAGAAACTT CTCGACAGAC GTCGCGGTGA GTTCAGGCTT TTTCATGGTG GCGGCTGGAT CGGTCGAAAG GCCCGGAGAT GAGGAAGAGG AGAACAGCGC GGCAGACGTG CGCTTTTGAA GCGTGCGAGA ATGCCGGGCC TCCGGAGGAC CTTCGGGCGC CCGCCCCGCC CCTGAGCCCG CCCCTGAGCC CGCCCCCGGA CCCACCCCTT CCCAGCCTCT GAGCCCAGAA AGCGAAGGAG CAAAGCTGCT ATTGGCCGCT GCCCCAAAGG CCTACCCGCT TCCATTGCTC AGCGGTGCTG TCCATCTGCA CGAGACTAGT GAGACGTGCT ACTTCCATTT GTCACGTCCT GCACGACGCG AGCTGCGGGG CGGGGGGGAA CTTCCTGACT AGGGGAGGAG TAGAAGGTGG CGCGAAGGGG CCACCAAAGA ACGGAGCCGG TTGGCGCCTA CCGGTGGATG TGGAATGTGT GCGAGGCCAG AGGCCACTTG TGTAGCGCCA AGTGCCAGCG GGGCTGCTAA AGCGCATGCT CCAGACTGCC TTGGGAAAAG CGCCTCCCCT ACCCGGTAGA ATTCATCGAT GATATCATAA CTTCGTATAA TGTATGCTAT ACGAAGTTAT GCGGCCCTCG AGGTC
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