pT7-6×His-LbCas12a-6×His
$99.00
In stock
SKU
PL22943
-
100μg liquid plasmid(Instantly):$142
| name | pT7-6×His-LbCas12a-6×His | another name | apT7-6×His-LbCas12a-6×His |
| Insert Size (bp) | 3684bp | Plasmid host | Escherichia coli |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Fluorescent labeling | ||
| Promoter | T7 | Replicon | pBR322 |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pET-28a | 5′ sequencing primer | T7 |
| 3′ sequencing primer | Copy number | Low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCAGCA GCCATCATCA TCATCATCAC AGCAGCGGCC TGGTGCCGCG CGGCAGCCAT ATGGCTAGCA TGACTGGTGG ACAGCAAATG GGTCGCGGAT CCATGAGCAA ACTGGAGAAG TTCACGAACT GTTATTCTCT GTCGAAAACC TTACGTTTTA AGGCCATTCC GGTAGGTAAA ACGCAAGAAA ATATCGACAA TAAACGTCTC CTGGTTGAAG ATGAAAAGCG TGCCGAAGAT TATAAAGGTG TTAAAAAGCT GCTTGATCGC TATTACCTGT CTTTTATCAA CGATGTTCTG CATTCCATCA AATTGAAAAA CTTGAATAAT TACATCTCGT TATTTCGCAA AAAAACCCGT ACCGAAAAAG AAAACAAGGA GCTGGAAAAC CTTGAAATTA ATCTGCGTAA AGAAATCGCG AAAGCATTTA AAGGTAATGA AGGATATAAA TCTCTGTTTA AAAAAGACAT TATTGAAACC ATCCTGCCTG AGTTCTTGGA TGATAAAGAT GAAATTGCCC TGGTTAATAG CTTTAATGGC TTTACGACTG CATTTACCGG CTTTTTTGAT AACCGTGAAA ACATGTTCAG CGAAGAAGCG AAATCGACTT CCATTGCCTT CCGTTGCATT AATGAAAACC TGACCCGCTA CATTTCTAAT ATGGACATCT TTGAAAAAGT TGATGCCATT TTCGACAAAC ATGAAGTTCA AGAAATCAAA GAAAAGATTT TAAACTCCGA TTATGATGTT GAAGATTTCT TTGAGGGAGA ATTTTTCAAT TTTGTTCTCA CCCAAGAGGG TATTGATGTC TATAACGCCA TTATCGGAGG ATTTGTTACG GAATCAGGCG AAAAGATTAA AGGACTGAAT GAATACATTA ATTTATACAA TCAGAAAACC AAACAGAAAT TGCCAAAATT CAAACCACTG TATAAACAGG TATTAAGCGA CCGTGAATCC TTGTCCTTTT ATGGCGAAGG CTATACAAGC GATGAAGAGG TGCTCGAAGT ATTCCGTAAT ACTTTGAACA AAAACTCGGA AATCTTCTCC AGCATTAAAA AACTGGAAAA ATTGTTTAAA AATTTCGATG AATATAGTAG CGCAGGCATC TTCGTCAAAA ATGGCCCTGC GATTTCAACC ATTTCTAAAG ACATTTTCGG TGAATGGAAC GTGATCCGCG ATAAATGGAA CGCGGAGTAT GACGACATTC ATCTGAAAAA AAAAGCCGTT GTTACCGAAA AGTATGAAGA TGACCGCCGC AAGAGCTTTA AAAAAATTGG TAGTTTCTCA CTGGAACAGT TACAAGAATA TGCAGACGCC GATCTGTCGG TTGTGGAAAA GTTAAAAGAG ATTATTATCC AGAAAGTCGA TGAAATTTAT AAAGTGTACG GCTCCAGCGA AAAGCTCTTT GATGCCGACT TTGTTTTAGA AAAATCGCTG AAAAAAAACG ATGCCGTGGT GGCCATCATG AAAGATCTGT TGGATTCGGT CAAATCGTTC GAAAACTATA TCAAGGCGTT CTTTGGCGAA GGGAAAGAGA CCAATCGTGA CGAATCGTTC TATGGCGATT TTGTGTTGGC GTATGATATT CTTCTGAAAG TAGATCACAT CTATGATGCT ATCCGCAACT ACGTGACCCA GAAACCGTAT TCGAAGGATA AATTTAAGCT GTATTTTCAA AACCCTCAGT TTATGGGTGG CTGGGATAAA GATAAAGAAA CCGACTACCG CGCGACGATC TTGCGCTATG GCTCTAAGTA CTATTTAGCC ATCATGGATA AAAAGTATGC AAAATGCCTC CAGAAAATCG ATAAGGATGA TGTCAACGGG AACTACGAAA AAATCAACTA CAAACTCTTG CCTGGCCCGA ATAAAATGCT GCCGAAAGTC TTTTTTTCCA AAAAATGGAT GGCCTATTAT AACCCTTCCG AAGATATTCA GAAGATTTAC AAGAACGGTA CCTTTAAAAA AGGTGACATG TTTAATCTGA ATGACTGTCA CAAGCTTATT GATTTCTTCA AAGATAGTAT TTCTCGCTAC CCTAAATGGT CAAACGCGTA CGATTTTAAT TTCTCAGAAA CAGAGAAATA CAAAGATATT GCGGGGTTTT ATCGCGAAGT CGAAGAACAA GGGTATAAGG TGTCGTTCGA ATCGGCTTCC AAAAAGGAAG TGGATAAACT TGTTGAAGAA GGCAAACTCT ATATGTTCCA GATCTACAAC AAAGATTTCT CCGACAAGAG CCATGGCACG CCGAACTTAC ATACCATGTA CTTTAAATTA CTGTTCGATG AAAATAATCA TGGTCAGATT CGCTTGAGCG GTGGCGCCGA ACTGTTTATG CGCCGCGCAT CATTGAAAAA GGAAGAACTT GTTGTGCACC CAGCAAACAG TCCGATTGCA AACAAAAATC CGGATAACCC GAAGAAGACT ACCACCTTAA GCTATGATGT ATATAAAGAT AAACGTTTTA GCGAAGATCA GTATGAACTC CACATTCCGA TCGCGATTAA CAAATGTCCT AAGAACATCT TTAAAATCAA TACCGAGGTT CGTGTACTGC TCAAACACGA CGACAACCCC TATGTTATTG GCATTGACCG CGGCGAACGC AACCTGCTGT ATATTGTCGT TGTGGATGGC AAAGGTAACA TTGTTGAACA GTATAGCCTT AATGAAATCA TCAATAATTT CAACGGCATT CGTATTAAAA CGGACTATCA TAGCCTGCTG GACAAAAAAG AGAAAGAACG CTTCGAAGCC CGTCAGAATT GGACCTCTAT CGAAAATATC AAGGAACTGA AAGCTGGGTA CATTTCTCAA GTGGTCCACA AGATTTGCGA ACTGGTGGAA AAGTATGACG CGGTGATCGC ACTGGAGGAT CTGAATTCGG GCTTTAAAAA TAGCCGTGTG AAGGTGGAGA AACAGGTTTA CCAAAAGTTC GAAAAAATGC TGATTGACAA ACTTAATTAC ATGGTGGACA AAAAATCTAA TCCATGTGCG ACCGGCGGGG CTCTCAAAGG CTATCAGATT ACGAATAAAT TCGAAAGCTT CAAATCCATG TCTACCCAGA ACGGATTTAT CTTTTACATC CCCGCCTGGC TGACTTCCAA AATTGATCCG AGTACCGGTT TTGTTAATCT GCTGAAAACC AAATATACGT CGATCGCCGA TTCAAAAAAA TTCATTAGCA GCTTCGACCG TATTATGTAC GTCCCGGAAG AAGACCTGTT CGAATTCGCC CTTGACTATA AAAATTTCTC GCGTACGGAT GCCGATTATA TCAAAAAATG GAAACTGTAC AGCTACGGCA ACCGCATCCG CATTTTTCGC AACCCAAAGA AGAACAACGT ATTTGACTGG GAGGAAGTTT GCTTGACGAG CGCTTACAAA GAGCTCTTTA ACAAATATGG GATCAATTAT CAGCAAGGGG ACATCCGTGC GTTGCTGTGT GAGCAGTCAG ATAAAGCGTT CTACAGCTCT TTCATGGCTT TAATGTCGCT GATGTTGCAG ATGCGCAATT CGATCACTGG CCGCACCGAT GTGGATTTCC TGATTAGCCC GGTCAAAAAT AGCGATGGCA TTTTTTACGA TTCACGTAAC TATGAAGCCC AGGAAAACGC TATTCTGCCG AAGAACGCTG ATGCGAACGG CGCGTACAAT ATTGCCCGTA AGGTACTGTG GGCAATTGGT CAGTTCAAGA AAGCGGAAGA TGAAAAGTTG GATAAGGTCA AAATTGCGAT CTCGAATAAA GAATGGCTGG AATATGCGCA GACCAGCGTG AAACATCTCG AGCACCACCA CCACCACCAC TGAGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGAT
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