NM_001165963.4；DEE6; DEE6A; DEE6B; DRVT; EIEE6; FEB3;FEB3A; FHM3; GEFSP2; HBSCI; NAC1; Nav1.1; SCN1; SMEI
100μg liquid plasmid(Instantly):$163
| name | pCMV-SCN1A(human-Toxic-2-opt)-3×FLAG-Neo | another name | apCMV-SCN1A(human-Toxic-2-opt)-3×FLAG-Neo |
| Insert Size (bp) | 6027bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pCMV-SCN1A(human-Toxic-opt)-3×FLAG-Neo | 5′ sequencing primer | CMV-F |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCATGGA ACAGACCGTG CTTGTTCCAC CAGGACCAGA TTCTTTCAAC TTTTTTACGA GGGAGTCCCT CGCCGCTATC GAACGAAGAA TTGCAGAGGA GAAGGCCAAG AACCCCAAAC CGGACAAAAA GGATGACGAT GAGAACGGTC CCAAACCTAA TTCAGACTTG GAGGCGGGAA AGAATCTGCC CTTCATCTAC GGGGATATTC CTCCTGAGAT GGTCAGCGAA CCATTGGAGG ACCTTGATCC ATACTACATA AACAAGAAGA CTTTCATCGT GCTTAATAAG GGTAAGGCCA TTTTCCGCTT TTCCGCTACC TCAGCTCTCT ACATCCTCAC GCCGTTCAAC CCACTGAGAA AGATCGCCAT AAAAATTCTC GTGCACAGCC TCTTCTCAAT GCTCATCATG TGCACCATTC TGACCAACTG CGTTTTTATG ACTATGTCTA ACCCACCTGA TTGGACCAAA AACGTGGAGT ATACCTTCAC AGGCATCTAC ACGTTCGAGA GCCTTATAAA GATCATTGCA AGGGGTTTTT GTCTTGAGGA CTTCACTTTC TTGCGCGATC CCTGGAATTG GCTGGACTTT ACTGTAATTA CATTCGCATA CGTGACAGAG TTCGTGGATC TGGGAAACGT AAGCGCTCTG AGGACTTTCC GGGTCTTGAG AGCCTTGAAG ACAATTTCAG TTATCCCGGG GCTGAAGACA ATCGTGGGCG CCTTGATTCA GTCAGTTAAA AAGCTGTCCG ACGTGATGAT ACTTACAGTA TTCTGTCTGA GCGTCTTCGC CCTTATCGGA CTGCAGTTGT TTATGGGTAA CCTCAGAAAT AAATGTATCC AATGGCCCCC CACTAATGCC AGCCTCGAAG AACACTCCAT CGAGAAAAAT ATTACCGTGA ACTACAACGG CACCCTTATT AACGAGACTG TGTTCGAGTT CGACTGGAAA TCTTACATTC AGGACAGTCG GTACCACTAC TTTCTGGAAG GATTTTTGGA TGCACTCCTG TGTGGAAACT CTTCTGATGC CGGTCAGTGT CCAGAGGGCT ATATGTGCGT TAAGGCCGGT AGAAATCCTA ACTACGGCTA TACCTCCTTC GATACCTTCT CATGGGCATT CCTGTCATTG TTTCGACTCA TGACCCAGGA TTTCTGGGAA AACCTGTACC AACTCACACT GCGCGCCGCC GGCAAAACTT ACATGATTTT CTTTGTTCTG GTTATATTTC TGGGGTCCTT CTACCTGATC AACCTCATCC TTGCTGTGGT GGCAATGGCA TATGAGGAGC AGAATCAGGC TACTCTGGAA GAGGCAGAAC AAAAAGAGGC TGAATTTCAG CAAATGATCG AACAGCTGAA GAAACAACAA GAAGCAGCAC AGCAGGCCGC CACAGCCACA GCTTCCGAAC ACTCTCGGGA ACCTTCAGCT GCAGGGAGAC TGAGTGACAG CAGCTCCGAA GCATCTAAAC TGTCTAGTAA GAGTGCCAAA GAAAGACGCA ACAGGAGGAA GAAGAGGAAA CAGAAAGAGC AGAGCGGAGG GGAGGAGAAG GATGAGGACG AGTTCCAGAA ATCCGAGTCT GAGGACTCAA TTAGACGCAA GGGGTTCCGG TTTTCCATCG AGGGCAATAG ATTGACCTAC GAGAAAAGGT ATAGCAGCCC TCATCAGTCA TTGCTCAGTA TTCGCGGCTC CCTGTTTAGT CCCCGCAGGA ACTCCAGGAC TTCCCTGTTT TCTTTCAGAG GCCGAGCCAA GGATGTGGGT TCAGAAAACG ACTTTGCTGA CGATGAGCAC TCTACCTTCG AAGATAACGA GTCTAGGAGA GATAGCCTTT TTGTCCCTAG AAGACACGGC GAAAGGCGCA ATAGCAACCT CAGCCAGACT AGCCGGAGCT CTAGAATGCT GGCAGTCTTT CCAGCCAACG GGAAGATGCA CTCAACCGTG GACTGTAATG GTGTGGTCTC ACTGGTTGGA GGGCCTTCTG TTCCCACTTC TCCTGTGGGA CAGTTGCTCC CCGAGGTAAT AATCGACAAA CCCGCCACCG ATGATAATGG CACGACGACA GAGACAGAAA TGAGGAAGCG GCGCTCATCA AGTTTTCACG TGTCCATGGA TTTTCTAGAA GATCCTTCCC AAAGGCAACG AGCAATGAGT ATAGCCAGCA TTCTAACAAA TACAGTAGAA GAACTTGAAG AATCCAGGCA GAAATGCCCA CCCTGTTGGT ATAAATTTTC CAACATATTC TTAATCTGGG ACTGTTCTCC ATATTGGTTA AAAGTGAAAC ATGTTGTCAA CCTGGTTGTG ATGGACCCAT TTGTTGACCT GGCCATCACC ATCTGTATTG TCTTAAATAC TCTTTTCATG GCCATGGAGC ACTATCCAAT GACGGACCAT TTCAATAATG TGCTTACAGT AGGAAACTTG GTTTTCACTG GGATCTTTAC AGCAGAAATG TTTCTGAAAA TTATTGCCAT GGATCCTTAC TATTATTTCC AAGAAGGCTG GAATATCTTT GACGGTTTTA TTGTGACGCT TAGCCTGGTA GAACTTGGAC TCGCCAATGT GGAAGGATTA TCTGTTCTCC GTTCATTTCG ATTGCTGCGA GTTTTCAAGT TGGCAAAATC TTGGCCAACG TTAAATATGC TAATAAAGAT CATCGGCAAT TCCGTGGGGG CTCTGGGAAA TTTAACCCTC GTCTTGGCCA TCATCGTCTT CATTTTTGCC GTGGTCGGCA TGCAGCTCTT TGGTAAAAGC TACAAAGATT GTGTCTGCAA GATCGCCAGT GATTGTCAAC TCCCACGCTG GCACATGAAT GACTTCTTCC ACTCCTTCCT GATTGTGTTC CGCGTGCTGT GTGGGGAGTG GATAGAGACC ATGTGGGACT GTATGGAGGT TGCTGGTCAA GCCATGTGCC TTACTGTCTT CATGATGGTC ATGGTGATTG GAAACCTAGT GGTCCTGAAT CTCTTTCTGG CCTTGCTTCT GAGCTCATTT AGTGCAGACA ACCTTGCAGC CACTGATGAT GATAATGAAA TGAATAATCT CCAAATTGCT GTGGATAGGA TGCACAAAGG AGTAGCTTAT GTGAAAAGAA AAATATATGA ATTTATTCAA CAGTCCTTCA TTAGGAAACA AAAGATTTTA GATGAAATTA AACCACTTGA TGATCTAAAC AACAAGAAAG ACAGTTGTAT GTCCAATCAT ACAGCAGAAA TTGGGAAAGA TCTTGACTAT CTTAAAGATG TAAATGGAAC TACAAGTGGT ATAGGAACTG GCAGCAGTGT TGAAAAATAC ATTATTGATG AAAGTGATTA CATGTCATTC ATAAACAACC CCAGTCTTAC TGTGACTGTA CCAATTGCTG TAGGAGAATC TGACTTTGAA AATTTAAACA CGGAAGACTT TAGTAGTGAA TCGGATCTGG AAGAAAGCAA AGAGAAACTG AATGAAAGCA GTAGCTCATC AGAAGGTAGC ACTGTGGACA TCGGCGCACC TGTAGAAGAA CAGCCCGTAG TGGAACCTGA AGAAACTCTT GAACCAGAAG CTTGTTTCAC TGAAGGCTGT GTACAAAGAT TCAAGTGTTG TCAAATCAAT GTGGAAGAAG GCAGAGGAAA ACAATGGTGG AACCTGAGAA GGACGTGTTT CCGAATAGTT GAACATAACT GGTTTGAGAC CTTCATTGTT TTCATGATTC TCCTTAGTAG TGGTGCTCTG GCATTTGAAG ATATATATAT TGATCAGCGA AAGACGATTA AGACGATGTT GGAATATGCT GACAAGGTTT TCACTTACAT TTTCATTCTG GAAATGCTTC TAAAATGGGT GGCATATGGC TATCAAACAT ATTTCACCAA TGCCTGGTGT TGGCTGGACT TCTTAATTGT TGATGTTTCA TTGGTCAGTT TAACAGCAAA TGCCTTGGGT TACTCAGAAC TTGGAGCCAT CAAATCTCTC AGGACACTAA GAGCTCTGAG ACCTCTAAGA GCCTTATCTC GATTTGAAGG GATGAGGGTG GTTGTGAATG CCCTTTTAGG AGCAATTCCA TCCATCATGA ATGTGCTTCT GGTTTGTCTT ATATTCTGGC TAATTTTCAG CATCATGGGC GTAAATTTGT TTGCTGGCAA ATTCTACCAC TGTATTAACA CCACAACTGG TGACAGGTTT GACATCGAAG ACGTGAATAA TCATACTGAT TGCCTAAAAC TAATAGAAAG AAATGAGACT GCTCGATGGA AAAATGTGAA AGTAAACTTT GATAATGTAG GATTTGGGTA TCTCTCTTTG CTTCAAGTTG CCACATTCAA AGGATGGATG GATATAATGT ATGCAGCAGT TGATTCCAGA AATGTGGAAC TCCAGCCTAA GTATGAAGAA AGTCTGTACA TGTATCTTTA CTTTGTTATT TTCATCATCT TTGGGTCCTT CTTCACCTTG AACCTGTTTA TTGGTGTCAT CATAGATAAT TTCAACCAGC AGAAAAAGAA GTTTGGAGGT CAAGACATCT TTATGACAGA AGAACAGAAG AAATACTATA ATGCAATGAA AAAATTAGGA TCGAAAAAAC CGCAAAAGCC TATACCTCGA CCAGGAAACA AATTTCAAGG AATGGTCTTT GACTTCGTAA CCAGACAAGT TTTTGACATA AGCATCATGA TTCTCATCTG TCTTAACATG GTCACAATGA TGGTGGAAAC AGATGACCAG AGTGAATATG TGACTACCAT TTTGTCACGC ATCAATCTGG TGTTCATTGT GCTATTTACT GGAGAGTGTG TACTGAAACT CATCTCTCTA CGCCATTATT ATTTTACCAT TGGATGGAAT ATTTTTGATT TTGTGGTTGT CATTCTCTCC ATTGTAGGTA TGTTTCTTGC CGAGCTGATA GAAAAGTATT TCGTGTCCCC TACCCTGTTC CGAGTGATCC GTCTTGCTAG GATTGGCCGA ATCCTACGTC TGATCAAAGG AGCAAAGGGG ATCCGCACGC TGCTCTTTGC TTTGATGATG TCCCTTCCTG CGTTGTTTAA CATCGGCCTC CTACTCTTCC TAGTCATGTT CATCTACGCC ATCTTTGGGA TGTCCAACTT TGCCTATGTT AAGAGGGAAG TTGGGATCGA TGACATGTTC AACTTTGAGA CCTTTGGCAA CAGCATGATC TGCCTATTCC AAATTACAAC CTCTGCTGGC TGGGATGGAT TGCTAGCACC CATTCTCAAC AGTAAGCCAC CCGACTGTGA CCCTAATAAA GTTAACCCTG GAAGCTCAGT TAAGGGAGAC TGTGGGAACC CATCTGTTGG AATTTTCTTT TTTGTCAGTT ACATCATCAT ATCCTTCCTG GTTGTGGTGA ACATGTACAT CGCGGTCATC CTGGAGAACT TCAGTGTTGC TACTGAAGAA AGTGCAGAGC CTCTGAGTGA GGATGACTTT GAGATGTTCT ATGAGGTTTG GGAGAAGTTT GATCCCGATG CAACTCAGTT CATGGAATTT GAAAAATTAT CTCAGTTTGC AGCTGCGCTT GAACCGCCTC TCAATCTGCC ACAACCAAAC AAACTCCAGC TCATTGCCAT GGATTTGCCC ATGGTGAGTG GTGACCGGAT CCACTGTCTT GATATCTTAT TTGCTTTTAC AAAGCGGGTT CTAGGAGAGA GTGGAGAGAT GGATGCTCTA CGAATACAGA TGGAAGAGCG ATTCATGGCT TCCAATCCTT CCAAGGTCTC CTATCAGCCA ATCACTACTA CTTTAAAACG AAAACAAGAG GAAGTATCTG CTGTCATTAT TCAGCGTGCT TACAGACGCC ACCTTTTAAA GCGAACTGTA AAACAAGCTT CCTTTACGTA CAATAAAAAC AAAATCAAAG GTGGGGCTAA TCTTCTTATA AAAGAAGACA TGATAATTGA CAGAATAAAT GAAAACTCTA TTACAGAAAA AACTGATCTG ACCATGTCCA CTGCAGCTTG TCCACCTTCC TATGACCGGG TGACAAAGCC AATTGTGGAA AAACATGAGC AAGAAGGCAA AGATGAAAAA GCCAAAGGGA AAGGATCCGA CTACAAAGAC CATGACGGTG ATTATAAAGA TCATGACATC GACTACAAGG ATGACGATGA CAAGTAGTGA GGGCCCGATA TCTCGACAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAGCTGACGT CCTTTCCATG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC AGACGAGTCG GATCTCCCTT TGGGCCGCCT CCCCGCCTGG AAACGGGGGA GGCTAACTGA AACACGGAAG GAGACAATAC CGGAAGGAAC CCGCGCTATG ACGGCAATAA AAAGACAGAA TAAAACGCAC GGGTGTTGGG TCGTTTGTTC ATAAACGCGG GGTTCGGTCC CAGGGCTGGC ACTCTGTCGA TACCCCACCG AGACCCCATT GGGGCCAATA CGCCCGCGTT TCTTCCTTTT CCCCACCCCA CCCCCCAAGT TCGGGTGAAG GCCCAGGGCT CGCAGCCAAC GTCGGGGCGG CAGGCCCTGC CATAGCAGAT CTGCGCAGCT GGGGCTCTAG GGGGTATCCC CACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGCA TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGGGGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT AATTCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCTGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCAATGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCGAC GGATCGGGAG ATCTCCCGAT CCCCTATGGT CGACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GG
