pLV2-CMV-C3(mouse)-3×FLAG-mCMV-ZsGreen1-Puro
$113.00
In stock
SKU
PL22841
NM_009778.3;ASP; HSE-MSF; Plp;
100μg liquid plasmid(Instantly):$134
| name | pLV2-CMV-C3(mouse)-3×FLAG-mCMV-ZsGreen1-Puro | another name | apLV2-CMV-C3(mouse)-3×FLAG-mCMV-ZsGreen1-Puro |
| Insert Size (bp) | 4989bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | pLV2-CMV-MCS-3×FLAG-mCMV-ZsGreen1-Puro | 5′ sequencing primer | CMV-F |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGGAAGGGCT AATTCACTCC CAAAGAAGAC AAGATATCCT TGATCTGTGG ATCTACCACA CACAAGGCTA CTTCCCTGAT TAGCAGAACT ACACACCAGG GCCAGGGGTC AGATATCCAC TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCC AGATAAGGTA GAAGAGGCCA ATAAAGGAGA GAACACCAGC TTGTTACACC CTGTGAGCCT GCATGGGATG GATGACCCGG AGAGAGAAGT GTTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC GTGGCCCGAG AGCTGCATCC GGAGTACTTC AAGAACTGCT GATATCGAGC TTGCTACAAG GGACTTTCCG CTGGGGACTT TCCAGGGAGG CGTGGCCTGG GCGGGACTGG GGAGTGGCGA GCCCTCAGAT CCTGCATATA AGCAGCTGCT TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCTCGACGG TATCGCCTTT AAAAGAAAAG GGGGGATTGG GGGGTACAGT GCAGGGGAAA GAATAGTAGA CATAATAGCA ACAGACATAC AAACTAAAGA ACTACAAAAA CAAATTACAA AAATTCAAAA TTTTCGGGTT TATTACAGGG ACAGCAGAGA TCCAGTTTAT CGATAAGCTT GGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA CGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACACCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCG TTTAGTGAAC CGTCAGATCG CCTGGAGACG CCATCCACGC TGTTTTGACC TCCATAGAAG ACACCGACTC TACTAGAGGA TCTATTTCCG GTGAATTCGC CACCATGGGA CCAGCTTCAG GGTCCCAGCT ACTAGTGCTA CTGCTGCTGT TGGCCAGCTC CCCATTAGCT CTGGGGATCC CCATGTATTC CATCATTACT CCCAATGTCC TACGGCTGGA GAGCGAAGAG ACCATCGTAC TGGAGGCCCA CGATGCTCAG GGTGACATCC CAGTCACAGT CACTGTGCAA GACTTCCTAA AGAGGCAAGT GCTGACCAGT GAGAAGACAG TGTTGACAGG AGCCAGTGGA CATCTGAGAA GCGTCTCCAT CAAGATTCCA GCCAGTAAGG AATTCAACTC AGATAAGGAG GGGCACAAGT ACGTGACAGT GGTGGCAAAC TTCGGGGAAA CGGTGGTGGA GAAAGCAGTG ATGGTAAGCT TCCAGAGTGG GTACCTCTTC ATCCAGACAG ACAAGACCAT CTACACCCCT GGCTCCACTG TCTTATATCG GATCTTCACT GTGGACAACA ACCTACTGCC CGTGGGCAAG ACAGTCGTCA TCCTCATTGA GACCCCCGAT GGCATTCCTG TCAAGAGAGA CATTCTGTCT TCCAACAACC AACACGGCAT CTTGCCTTTG TCTTGGAACA TTCCTGAACT GGTCAACATG GGGCAGTGGA AGATCCGAGC CTTTTACGAA CATGCGCCGA AGCAGATCTT CTCCGCAGAG TTTGAGGTGA AGGAATACGT GCTGCCCAGT TTTGAGGTCC GGGTGGAGCC CACAGAGACA TTTTATTACA TCGATGACCC AAATGGCCTG GAAGTTTCCA TCATAGCCAA GTTCCTGTAC GGGAAAAACG TGGACGGGAC AGCCTTCGTG ATTTTTGGGG TCCAGGATGG CGATAAGAAG ATTTCTCTGG CCCACTCCCT CACGCGCGTA GTGATTGAGG ATGGTGTGGG GGATGCAGTG CTGACCCGGA AGGTGCTGAT GGAGGGGGTA CGGCCTTCCA ACGCCGACGC CCTGGTGGGG AAGTCCCTGT ATGTCTCCGT CACTGTCATC CTGCACTCAG GTAGTGACAT GGTAGAGGCA GAGCGCAGTG GGATCCCGAT TGTCACTTCC CCGTACCAGA TCCACTTCAC CAAGACACCC AAATTCTTCA AGCCAGCCAT GCCCTTTGAC CTCATGGTGT TCGTGACCAA CCCCGATGGC TCTCCGGCCA GCAAAGTGCT GGTGGTCACT CAGGGATCTA ATGCAAAGGC TCTCACCCAA GATGATGGCG TGGCCAAGCT AAGCATCAAC ACACCCAACA GCCGCCAACC CCTGACCATC ACAGTCCGCA CCAAGAAGGA CACTCTCCCA GAATCACGGC AGGCCACCAA GACAATGGAG GCCCATCCCT ACAGCACTAT GCACAACTCC AACAACTACC TACACTTGTC AGTGTCACGA ATGGAGCTCA AGCCGGGGGA CAACCTCAAT GTCAACTTCC ACCTGCGCAC AGACCCAGGC CATGAGGCCA AGATCCGATA CTACACCTAC CTGGTTATGA ACAAGGGGAA GCTCCTGAAG GCAGGCCGCC AGGTTCGGGA GCCTGGCCAG GACCTGGTGG TCTTGTCCCT GCCCATCACT CCAGAGTTTA TTCCTTCATT TCGCCTGGTG GCTTACTACA CCCTGATTGG AGCTAGTGGC CAGAGGGAGG TGGTGGCTGA CTCTGTGTGG GTGGATGTGA AGGATTCCTG TATTGGCACG CTGGTGGTGA AGGGTGACCC AAGAGATAAC CATCTCGCAC CTGGGCAACA AACGACACTC AGGATTGAAG GAAACCAGGG GGCCCGAGTG GGGCTAGTGG CTGTGGACAA GGGAGTGTTT GTGCTGAACA AGAAGAACAA ACTCACACAG AGCAAGATCT GGGATGTGGT AGAGAAGGCA GACATTGGCT GCACCCCAGG CAGTGGGAAG AACTATGCTG GTGTCTTCAT GGATGCAGGC CTGGCCTTCA AGACAAGCCA AGGACTGCAG ACTGAACAGA GAGCAGATCT TGAGTGCACC AAGCCAGCAG CCCGCCGCCG TCGCTCAGTA CAGTTGATGG AAAGAAGGAT GGACAAAGCT GGTCAGTACA CTGACAAGGG TCTTCGGAAG TGTTGTGAGG ATGGTATGCG GGATATCCCT ATGAGATACA GCTGCCAGCG CCGGGCACGC CTCATCACCC AGGGCGAGAA CTGCATAAAG GCCTTCATAG ACTGCTGCAA CCACATCACC AAGCTGCGTG AACAACACAG AAGAGACCAC GTGCTGGGCC TGGCCAGGAG TGAATTGGAG GAAGACATAA TTCCAGAAGA AGATATTATC TCTAGAAGCC ACTTCCCACA GAGCTGGTTG TGGACCATAG AAGAGTTGAA AGAACCAGAG AAAAATGGAA TCTCTACGAA GGTCATGAAC ATCTTTCTCA AAGATTCCAT CACCACCTGG GAGATTCTGG CAGTGAGCTT GTCAGACAAG AAAGGGATCT GTGTGGCAGA CCCCTATGAG ATCAGAGTGA TGCAGGACTT CTTCATTGAC CTGCGGCTGC CCTACTCTGT AGTGCGCAAC GAACAGGTGG AGATCAGAGC TGTGCTCTTC AACTACCGTG AACAGGAGGA ACTTAAGGTG AGGGTGGAAC TGTTGCATAA TCCAGCCTTC TGCAGCATGG CCACCGCCAA GAATCGCTAC TTCCAGACCA TCAAAATCCC TCCCAAGTCC TCGGTGGCTG TACCGTATGT CATTGTCCCC TTGAAGATCG GCCAACAAGA GGTGGAGGTC AAGGCTGCTG TCTTCAATCA CTTCATCAGT GATGGTGTCA AGAAGACACT GAAGGTCGTG CCAGAAGGAA TGAGAATCAA CAAAACTGTG GCCATCCATA CACTGGACCC AGAGAAGCTC GGTCAAGGGG GAGTGCAGAA GGTGGATGTG CCTGCCGCAG ACCTTAGCGA CCAAGTGCCA GACACAGACT CTGAGACCAG AATTATCCTG CAAGGGAGCC CGGTGGTTCA GATGGCTGAA GATGCTGTGG ACGGGGAGCG GCTGAAACAC CTGATCGTGA CCCCCGCAGG CTGTGGGGAA CAGAACATGA TTGGCATGAC ACCAACAGTC ATTGCGGTAC ACTACCTGGA CCAGACCGAA CAGTGGGAGA AGTTCGGCAT AGAGAAGAGG CAAGAGGCCC TGGAGCTCAT CAAGAAAGGG TACACCCAGC AGCTGGCCTT CAAACAGCCC AGCTCTGCCT ATGCTGCCTT CAACAACCGG CCCCCCAGCA CCTGGCTGAC AGCCTACGTG GTCAAGGTCT TCTCTCTAGC TGCCAACCTC ATCGCCATCG ACTCTCACGT CCTGTGTGGG GCTGTTAAAT GGTTGATTCT GGAGAAACAG AAGCCGGATG GTGTCTTTCA GGAGGATGGG CCCGTGATTC ACCAAGAAAT GATTGGTGGC TTCCGGAACG CCAAGGAGGC AGATGTGTCA CTCACAGCCT TCGTCCTCAT CGCACTGCAG GAAGCCAGGG ACATCTGTGA GGGGCAGGTC AATAGCCTTC CTGGGAGCAT CAACAAGGCA GGGGAGTATA TTGAAGCCAG TTACATGAAC CTGCAGAGAC CATACACAGT GGCCATTGCT GGGTATGCCC TGGCCCTGAT GAACAAACTG GAGGAACCTT ACCTCGGCAA GTTTCTGAAC ACAGCCAAAG ATCGGAACCG CTGGGAGGAG CCTGACCAGC AGCTCTACAA CGTAGAGGCC ACATCCTACG CCCTCCTGGC CCTGCTGCTG CTGAAAGACT TTGACTCTGT GCCCCCTGTA GTGCGCTGGC TCAATGAGCA AAGATACTAC GGAGGCGGCT ATGGCTCCAC CCAGGCTACC TTCATGGTAT TCCAAGCCTT GGCCCAATAT CAAACAGATG TCCCTGACCA TAAGGACTTG AACATGGATG TGTCCTTCCA CCTCCCCAGC CGTAGCTCTG CAACCACGTT TCGCCTGCTC TGGGAAAATG GCAACCTCCT GCGATCGGAA GAGACCAAGC AAAATGAGGC CTTCTCTCTA ACAGCCAAAG GAAAAGGCCG AGGCACATTG TCGGTGGTGG CAGTGTATCA TGCCAAACTC AAAAGCAAAG TCACCTGCAA GAAGTTTGAC CTCAGGGTCA GCATAAGACC AGCCCCTGAG ACAGCCAAGA AGCCCGAGGA AGCCAAGAAT ACCATGTTCC TTGAAATCTG CACCAAGTAC TTGGGAGATG TGGACGCCAC TATGTCCATC CTGGACATCT CCATGATGAC TGGCTTTGCT CCAGACACAA AGGACCTGGA ACTGCTGGCC TCTGGAGTAG ATAGATACAT CTCCAAGTAC GAGATGAACA AAGCCTTCTC CAACAAGAAC ACCCTCATCA TCTACCTAGA AAAGATTTCA CACACCGAAG AAGACTGCCT GACCTTCAAA GTTCACCAGT ACTTTAATGT GGGACTTATC CAGCCCGGGT CGGTCAAGGT CTACTCCTAT TACAACCTCG AGGAATCATG CACCCGGTTC TATCATCCAG AGAAGGACGA TGGGATGCTC AGCAAGCTGT GCCACAGTGA AATGTGCCGG TGTGCTGAAG AGAACTGCTT CATGCAACAG TCACAGGAGA AGATCAACCT GAATGTCCGG CTAGACAAGG CTTGTGAGCC CGGAGTCGAC TATGTGTACA AGACCGAGCT AACCAACATA GAGCTGTTGG ATGATTTTGA TGAGTACACC ATGACCATCC AGCAGGTCAT CAAGTCAGGC TCAGATGAGG TGCAGGCAGG GCAGCAACGC AAGTTCATCA GCCACATCAA GTGCAGAAAC GCCCTGAAGC TGCAGAAAGG GAAGAAGTAC CTCATGTGGG GCCTCTCCTC TGACCTCTGG GGAGAAAAGC CCAACACCAG CTACATCATT GGGAAGGACA CGTGGGTGGA GCACTGGCCT GAGGCAGAAG AATGCCAGGA TCAGAAGTAC CAGAAACAGT GCGAAGAACT TGGGGCATTC ACAGAATCTA TGGTGGTTTA TGGTTGTCCC AACACTAGTA TTTGCGGCCG CGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC ATCGATTACA AGGATGACGA TGACAAGTGA GGATCCGATA TACTGAGTCA TTAGGGACTT TCCAATGGGT TTTGCCCAGT ACATAAGGTC AATAGGGGTG AATCAACAGG AAAGTCCCAT TGGAGCCAAG TACACTGAGT CAATAGGGAC TTTCCATTGG GTTTTGCCCA GTACAAAAGG TCAATAGGGG GTGAGTCAAT GGGTTTTTCC CATTATTGGC ACGTACATAA GGTCAATAGG GGTGAGTCAT TGGGTTTTTC CAGCCAATTT AATTAAAACG CCATGTACTT TCCCACCATT GACGTCAATG GGCTATTGAA ACTAATGCAA CGTGACCTTT AAACGGTACT TTCCCATAGC TGATTAATGG GAAAGTACCG TTCTCGAGCC AATACACGTC AATGGGAAGT GAAAGGGCAG CCAAAACGTA ACACCGCCCC GGTTTTCCCC TGGAAATTCC ATATTGGCAC GCATTCTATT GGCTGAGCTG CGTTCTACGT GGGTATAAGA GGCGCGACCA GCGTCGGTAC CGTCGCAGTC TTCGGTCTGA CCACCGTAGA ACGCAGATCA CCGGTCGCCA CCATGGCCCA GTCCAAGCAC GGCCTGACCA AGGAGATGAC CATGAAGTAC CGCATGGAGG GCTGCGTGGA CGGCCACAAG TTCGTGATCA CCGGCGAGGG CATCGGCTAC CCCTTCAAGG GCAAGCAGGC CATCAACCTG TGCGTGGTGG AGGGCGGCCC CTTGCCCTTC GCCGAGGACA TCTTGTCCGC CGCCTTCATG TACGGCAACC GCGTGTTCAC CGAGTACCCC CAGGACATCG TCGACTACTT CAAGAACTCC TGCCCCGCCG GCTACACCTG GGACCGCTCC TTCCTGTTCG AGGACGGCGC CGTGTGCATC TGCAACGCCG ACATCACCGT GAGCGTGGAG GAGAACTGCA TGTACCACGA GTCCAAGTTC TACGGCGTGA ACTTCCCCGC CGACGGCCCC GTGATGAAGA AGATGACCGA CAACTGGGAG CCCTCCTGCG AGAAGATCAT CCCCGTGCCC AAGCAGGGCA TCTTGAAGGG CGACGTGAGC ATGTACCTGC TGCTGAAGGA CGGTGGCCGC TTGCGCTGCC AGTTCGACAC CGTGTACAAG GCCAAGTCCG TGCCCCGCAA GATGCCCGAC TGGCACTTCA TCCAGCACAA GCTGACCCGC GAGGACCGCA GCGACGCCAA GAACCAGAAG TGGCACCTGA CCGAGCACGC CATCGCCTCC GGCTCCGCCT TGCCCTAAGG CGCGCCCGCC CCTCTCCCTC CCCCCCCCCT AACGTTACTG GCCGAAGCCG CTTGGAATAA GGCCGGTGTG CGTTTGTCTA TATGTTATTT TCCACCATAT TGCCGTCTTT TGGCAATGTG AGGGCCCGGA AACCTGGCCC TGTCTTCTTG ACGAGCATTC CTAGGGGTCT TTCCCCTCTC GCCAAAGGAA TGCAAGGTCT GTTGAATGTC GTGAAGGAAG CAGTTCCTCT GGAAGCTTCT TGAAGACAAA CAACGTCTGT AGCGACCCTT TGCAGGCAGC GGAACCCCCC ACCTGGCGAC AGGTGCCTCT GCGGCCAAAA GCCACGTGTA TAAGATACAC CTGCAAAGGC GGCACAACCC CAGTGCCACG TTGTGAGTTG GATAGTTGTG GAAAGAGTCA AATGGCTCAC CTCAAGCGTA TTCAACAAGG GGCTGAAGGA TGCCCAGAAG GTACCCCATT GTATGGGATC TGATCTGGGG CCTCGGTGCA CATGCTTTAC ATGTGTTTAG TCGAGGTTAA AAAACGTCTA GGCCCCCCGA ACCACGGGGA CGTGGTTTTC CTTTGAAAAA CACGATGATA ATACCGGTAT GACCGAGTAC AAGCCCACGG TGCGCCTCGC CACCCGCGAC GACGTCCCCA GGGCCGTACG CACCCTCGCC GCCGCGTTCG CCGACTACCC CGCCACGCGC CACACCGTCG ATCCGGACCG CCACATCGAG CGGGTCACCG AGCTGCAAGA ACTCTTCCTC ACGCGCGTCG GGCTCGACAT CGGCAAGGTG TGGGTCGCGG ACGACGGCGC CGCGGTGGCG GTCTGGACCA CGCCGGAGAG CGTCGAAGCG GGGGCGGTGT TCGCCGAGAT CGGCCCGCGC ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC GCGCAGCAAC AGATGGAAGG CCTCCTGGCG CCGCACCGGC CCAAGGAGCC CGCGTGGTTC CTGGCCACCG TCGGCGTCTC GCCCGACCAC CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG CTCCCCGGAG TGGAGGCGGC CGAGCGCGCC GGGGTGCCCG CCTTCCTGGA GACCTCCGCG CCCCGCAACC TCCCCTTCTA CGAGCGGCTC GGCTTCACCG TCACCGCCGA CGTCGAGGTG CCCGAAGGAC CGCGCACCTG GTGCATGACC CGCAAGCCCG GTGCCTGAAC GCGTCTGGAA CAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAGCTG ACGTCCTTTC CATGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC CCTTTGGGCC GCCTCCCCGC CTGGAATTAA TTCTGCAGTC GAGACCTAGA AAAACATGGA GCAATCACAA GTAGCAATAC AGCAGCTACC AATGCTGATT GTGCCTGGCT AGAAGCACAA GAGGAGGAGG AGGTGGGTTT TTCCAGTCAC ACCTCAGGTA CCTTTAAGAC CAATGACTTA CAAGGCAGCT GTAGATCTTA GCCACTTTTT AAAAGAAAAG AGGGGACTGG AAGGGCTAAT TCACTCCCAA CGAAGACAAG ATATCCTTGA TCTGTGGATC TACCACACAC AAGGCTACTT CCCTGATTAG CAGAACTACA CACCAGGGCC AGGGGTCAGA TATCCACTGA CCTTTGGATG GTGCTACAAG CTAGTACCAG TTGAGCCAGA TAAGGTAGAA GAGGCCAATA AAGGAGAGAA CACCAGCTTG TTACACCCTG TGAGCCTGCA TGGGATGGAT GACCCGGAGA GAGAAGTGTT AGAGTGGAGG TTTGACAGCC GCCTAGCATT TCATCACGTG GCCCGAGAGC TGCATCCGGA GTACTTCAAG AACTGCTGAT ATCGAGCTTG CTACAAGGGA CTTTCCGCTG GGGACTTTCC AGGGAGGCGT GGCCTGGGCG GGACTGGGGA GTGGCGAGCC CTCAGATCCT GCATATAAGC AGCTGCTTTT TGCCTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT CAGTGTGGAA AATCTCTAGC AGTAGTAGTT CATGTCATCT TATTATTCAG TATTTATAAC TTGCAAAGAA ATGAATATCA GAGAGTGAGA GGCCTTGACA TTGCTAGCGT TTTACCGTCG ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCGACGGA TCGGGAGATC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GGATCAACTG GATAACTCAA GCTAACCAAA ATCATCCCAA ACTTCCCACC CCATACCCTA TTACCACTGC CAATTACCTA GTGGTTTCAT TTACTCTAAA CCTGTGATTC CTCTGAATTA TTTTCATTTT AAAGAAATTG TATTTGTTAA ATATGTACTA CAAACTTAGT AGTTTTTAAA GAAATTGTAT TTGTTAAATA TGTACTACAA ACTTAGTAGT
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