pMAK705
$55.00
In stock
SKU
PL22748
-
100μg liquid plasmid(Instantly):$76
| name | pMAK705 | another name | apMAK705 |
| Insert Size (bp) | Plasmid host | Bacteria | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Chl |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pSC101 | |
| Competence | DH5a | Growth Temperature | 30°C |
| Vector backbone | pSUP202，pRK415，pGRG37 | 5′ sequencing primer | M13F |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GATCCCCGAC AGTAAGACGG GTAAGCCTGT TGATGATACC GCTGCCTTAC TGGGTGCATT AGCCAGTCTG AATGACCTGT CACGGGATAA TCCGAAGTGG TCAGACTGGA AAATCAGAGG GCAGGAACTG CTGAACAGCA AAAAGTCAGA TAGCACCACA TAGCAGACCC GCCATAAAAC GCCCTGAGAA GCCCGTGACG GGCTTTTCTT GTATTATGGG TAGTTTCCTT GCATGAATCC ATAAAAGGCG CCTGTAGTGC CATTTACCCC CATTCACTGC CAGAGCCGTG AGCGCAGCGA ACTGAATGTC ACGAAAAAGA CAGCGACTCA GGTGCCTGAT GGTCGGAGAC AAAAGGAATA TTCAGCGATT TGCCCGAGCT TGCGAGGGTG CTACTTAAGC CTTTAGGGTT TTAAGGTCTG TTTTGTAGAG GAGCAAACAG CGTTTGCGAC ATCCTTTTGT AATACTGCGG AACTGACTAA AGTAGTGAGT TATACACAGG GCTGGGATCT ATTCTTTTTA TCTTTTTTTA TTCTTTCTTT ATTCTATAAA TTATAACCAC TTGAATATAA ACAAAAAAAA CACACAAAGG TCTAGCGGAA TTTACAGAGG GTCTAGCAGA ATTTACAAGT TTTCCAGCAA AGGTCTAGCA GAATTTACAG ATACCCACAA CTCAAAGGAA AAGGACTAGT AATTATCATT GACTAGCCCA TCTCAATTGG TATAGTGATT AAAATCACCT AGACCAATTG AGATGTATGT CTGAATTAGT TGTTTTCAAA GCAAATGAAC TAGCGATTAG TCGCTATGAC TTAACGGAGC ATGAAACCAA GCTAATTTTA TGCTGTGTGG CACTACTCAA CCCCACGATT GAAAACCCTA CAAGGAAAGA ACGGACGGTA TCGTTCACTT ATAACCAATA CGTTCAGATG ATGAACATCA GTAGGGAAAA TGCTTATGGT GTATTAGCTA AAGCAACCAG AGAGCTGATG ACGAGAACTG TGGAAATCAG GAATCCTTTG GTTAAAGGCT TTGAGATTTT CCAGTGGACA AACTATGCCA AGTTCTCAAG CGAAAAATTA GAATTAGTTT TTAGTGAAGA GATATTGCCT TATCTTTTCC AGTTAAAAAA ATTCATAAAA TATAATCTGG AACATGTTAA GTCTTTTGAA AACAAATACT CTATGAGGAT TTATGAGTGG TTATTAAAAG AACTAACACA AAAGAAAACT CACAAGGCAA ATATAGAGAT TAGCCTTGAT GAATTTAAGT TCATGTTAAT GCTTGAAAAT AACTACCATG AGTTTAAAAG GCTTAACCAA TGGGTTTTGA AACCAATAAG TAAAGATTTA AACACTTACA GCAATATGAA ATTGGTGGTT GATAAGCGAG GCCGCCCGAC TGATACGTTG ATTTTCCAAG TTGAACTAGA TAGACAAATG GATCTCGTAA CCGAACTTGA GAACAACCAG ATAAAAATGA ATGGTGACAA AATACCAACA ACCATTACAT CAGATTCCTA CCTACATAAC GGACTAAGAA AAACACTACA CGATGCTTTA ACTGCAAAAA TTCAGCTCAC CAGTTTTGAG GCAAAATTTT TGAGTGACAT GCAAAGTAAG TATGATCTCA ATGGTTCGTT CTCATGGCTC ACGCAAAAAC AACGAACCAC ACTAGAGAAC ATACTGGCTA AATACGGAAG GATCTGAGGT TCTTATGGCT CTTGTATCTA TCAGTGAAGC ATCAAGACTA ACAAACAAAA GTAGAACAAC TGTTCACCGT TACATATCAA AGGGAAAACT GTCCATATGC ACAGATGAAA ACGGTGTAAA AAAGATAGAT ACATCAGAGC TTTTACGAGT TTTTGGTGCA TTTAAAGCTG TTCACCATGA ACAGATCGAC AATGTAACAG ATGAACAGCA TGTAACACCT AATAGAACAG GTGAAACCAG TAAAACAAAG CAACTAGAAC ATGAAATTGA ACACCTGAGA CAACTTGTTA CAGCTCAACA GTCACACATA GACAGCCTGA AACAGGCGAT GCTGCTTATC GAATCAAAGC TGCCGACAAC ACGGGAGCCA GTGACGCCTC CCGTGGGGAA AAAATCATGG CAATTCTGGA AGAAATAGCG CTTTCAGCCG GCAAACCGGC TGAAGCCGGA TCTGCGATTC TGATAACAAA CTAGCAACAC CAGAACAGCC CGTTTGCGGG CAGCAAAACC CGTACTTTTG GACGTTCCGG CGGTTTTTTG TGGCGAGTGG TGTTCGGGCG GTGCGCGCAA GATCCATTAT GTTAAACGGG CGAGTTTACA TCTCAAAACC GCCCGCTTAA CACCATCAGA AATCCTCAGC GCGATTTTAA GCACCAACCC CCCCCCGTAA CACCCAAATC CATACTGAAA GTGGCTTTGT TGAATAAATC GAACTTTTGC TGAGTTGAAG GATCAGATCA CGCATCCTCC CGACAACACA GACCATTCCG TGGCAAAGCA AAAGTTCAGA ATCACCAACT GGTCCACCTA CAACAAAGCT CTCATCAACC GTGGCTCCCT CACTTTCTGG CTGGATGATG AGGCGATTCA GGCCTGGTAT GAGTCGGCAA CACCTTCATC ACGAGGAAGG CCCCAGCGCT ATTCTGATCT CGCCATCACC ACCGTTCTGG TGATTAAACG CGTATTCCGG CTGACCCTGC GGGCTGCGCA GGGTTTTATT GATTCCATTT TTGCCCTGAT GAACGTTCCG TTGCGCTGCC CGGATTACAC CAGTGTCAGT AAGCGGGCAA AGTCGGTTAA TGTCAGTTTC AAAACGTCCA CCCGGGGTGA AATCGCACAC CTGGTGATTG ATTCCACCGG GCTGAAGGTC TTTGGTGAAG GCGAATGGAA AGTCAGAAAG CACGGCAAAG AGCGCCGTCG TATCTGGCGA AAGTTGCATC TTGCTGTGGG GATCAACACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGTC TTCGAATAAA TACCTGTGAC GGAAGATCAC TTCGCAGAAT AAATAAATCC TGGTGTCCCT GTTGATACCG GGAAGCCCTG GGCCAACTTT TGGCGAAAAT GAGACGTTGA TCGGCACGTA AGAGGTTCCA ACTTTCACCA TAATGAAATA AGATCACTAC CGGGCGTATT TTTTGAGTTA TCGAGATTTT CAGGAGCTAA GGAAGCTAAA ATGGAGAAAA AAATCACTGG ATATACCACC GTTGATATAT CCCAATGGCA TCGTAAAGAA CATTTTGAGG CATTTCAGTC AGTTGCTCAA TGTACCTATA ACCAGACCGT TCAGCTGGAT ATTACGGCCT TTTTAAAGAC CGTAAAGAAA AATAAGCACA AGTTTTATCC GGCCTTTATT CACATTCTTG CCCGCCTGAT GAATGCTCAT CCGGAATTCC GTATGGCAAT GAAAGACGGT GAGCTGGTGA TATGGGATAG TGTTCACCCT TGTTACACCG TTTTCCATGA GCAAACTGAA ACGTTTTCAT CGCTCTGGAG TGAATACCAC GACGATTTCC GGCAGTTTCT ACACATATAT TCGCAAGATG TGGCGTGTTA CGGTGAAAAC CTGGCCTATT TCCCTAAAGG GTTTATTGAG AATATGTTTT TCGTCTCAGC CAATCCCTGG GTGAGTTTCA CCAGTTTTGA TTTAAACGTG GCCAATATGG ACAACTTCTT CGCCCCCGTT TTCACCATGG GCAAATATTA TACGCAAGGC GACAAGGTGC TGATGCCGCT GGCGATTCAG GTTCATCATG CCGTTTGTGA TGGCTTCCAT GTCGGCAGAA TGCTTAATGA ATTACAACAG TACTGCGATG AGTGGCAGGG CGGGGCGTAA TTTTTTTAAG GCAGTTATTG GTGCCCTTAA ACGCCTGGTT GCTACGCCTG AATAAGTGAT AATAAGCGGA TGAATGGCAG AAATTCGAAA GCAAATTCGA CCCGGTCGTC GGTTCAGGGC AGGGTCGTTA AATAGCCGCT TATGTCTATT GCTGGTTTAC CGGTTTATTG ACTACCGGAA GCAGTGTGAC CGTGTGCTTC TCAAATGCCT GAGGCCAGTT TGCTCAGGCT CTCCCCGTGG AGGTAATAAT TGACGATATG ATCATTTATT CTGCCTCCCA GAGCCTGATA AAAACGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAATGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCATG TTTGACAGCT TATCATCGAT AAGCTCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGAATTCGAG CTCGGTACCC GGGGATCCTC TAGAGTCGAC CTGCAGGCAT GCAAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGCACC ATTCCTTGCG GCGGCGGTGC TCAACGGCCT CAACCTACTA CTGGGCTGCT TCCTAATGCA GGAGTCGCAT AAGGGAGAGC GTC
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