pSEVA231-Gen
$55.00
In stock
SKU
PL22404
-
100μg liquid plasmid(Instantly):$76
| name | pSEVA231-Gen | another name | apSEVA231-Gen |
| Insert Size (bp) | Plasmid host | Bacteria | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Empty vector | Prokaryotic resistance | Gen |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pBBR1 | |
| Competence | DH5a | Growth Temperature | 30°C |
| Vector backbone | pMRE132,pSEVA241,pBAD-MCS-pBBR1-Cam | 5′ sequencing primer | |
| 3′ sequencing primer | rrnBT1-R | Copy number | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TTAATTAAAG CGGATAACAA TTTCACACAG GAGGCCGCCT AGGCCGCGGC CGCGCGAATT CGAGCTCGGT ACCCGGGGAT CCTCTAGAGT CGACCTGCAG GCATGCAAGC TTGCGGCCGC GTCGTGACTG GGAAAACCCT GGCGACTAGT CTTGGACTCC TGTTGATAGA TCCAGTAATG ACCTCAGAAC TCCATCTGGA TTTGTTCAGA ACGCTCGGTT GCCGCCGGGC GTTTTTTATT GGTGAGAATC CAGGGGTCCC CAATAATTAC GATTTAAATT TGTGTCTCAA AATCTCTGAT GTTACATTGC ACAAGATAAA AATATATCAT CATGAACAAT AAAACTGTCT GCTTACATAA ACAGTAATAC AAGGGGTGTT ATGGAAGCCA TCACAAACGG CATGATGAAC CTGAATCGCC AGCGGCATCA GCACCTTGTC GCCTTGCGTA TAATATTTGC CCATGGACGC ACACCGTGGA AACGGATGAA GGCACGAACC CAGTTGACAT AAGCCTGTTC GGTTCGTAAA CTGTAATGCA AGTAGCGTAT GCGCTCACGC AACTGGTCCA GAACCTTGAC CGAACGCAGC GGTGGTAACG GCGCAGTGGC GGTTTTCATG GCTTGTTATG ACTGTTTTTT TGTACAGTCT ATGCCTCGGG CATCCAAGCA GCAAGCGCGT TACGCCGTGG GTCGATGTTT GATGTTATGG AGCAGCAACG ATGTTACGCA GCAGCAACGA TGTTACGCAG CAGGGCAGTC GCCCTAAAAC AAAGTTAGGT GGCTCAAGTA TGGGCATCAT TCGCACATGT AGGCTCGGCC CTGACCAAGT CAAATCCATG CGGGCTGCTC TTGATCTTTT CGGTCGTGAG TTCGGAGACG TAGCCACCTA CTCCCAACAT CAGCCGGACT CCGATTACCT CGGGAACTTG CTCCGTAGTA AGACATTCAT CGCGCTTGCT GCCTTCGACC AAGAAGCGGT TGTTGGCGCT CTCGCGGCTT ACGTTCTGCC CAGGTTTGAG CAGCCGCGTA GTGAGATCTA TATCTATGAT CTCGCAGTCT CCGGCGAGCA CCGGAGGCAG GGCATTGCCA CCGCGCTCAT CAATCTCCTC AAGCATGAGG CCAACGCGCT TGGTGCTTAT GTGATCTACG TGCAAGCAGA TTACGGTGAC GATCCCGCAG TGGCTCTCTA TACAAAGTTG GGCATACGGG AAGAAGTGAT GCACTTTGAT ATCGACCCAA GTACCGCCAC CTAACAATTC GTTCAAGCCG AGATCGGCTT CCCGTAATTA ATTGGACCGC GGTCCGCGCG TTGTCCTTTT CCGCTGCATA ACCCTGCTTC GGGGTCATTA TAGCGATTTT TTCGGTATAT CCATCCTTTT TCGCACGATA TACAGGATTT TGCCAAAGGG TTCGTGTAGA CTTTCCTTGG TGTATCCAAC GGCGTCAGCC GGGCAGGATA GGTGAAGTAG GCCCACCCGC GAGCGGGTGT TCCTTCTTCA CTGTCCCTTA TTCGCACCTG GCGGTGCTCA ACGGGAATCC TGCTCTGCGA GGCTGGCCGT AGGCCGGCCC TACCGGCGCG GCAGCGTTAC CCGTGTCGGC GGCTCCAACG GCTCGCCATC GTCCAGAAAA CACGGCTCAT CGGGCATCGG CAGGCGCTGC TGCCCGCGCC GTTCCCATTC CTCCGTTTCG GTCAAGGCTG GCAGGTCTGG TTCCATGCCC GGAATGCCGG GCTGGCTGGG CGGCTCCTCG CCGGGGCCGG TCGGTAGTTG CTGCTCGCCC GGATACAGGG TCGGGATGCG GCGCAGGTCG CCATGCCCCA ACAGCGATTC GTCCTGGTCG TCGTGATCAA CCACCACGGC GGCACTGAAC ACCGACAGGC GCAACTGGTC GCGGGGCTGG CCCCACGCCA CGCGGTCATT GACCACGTAG GCCGACACGG TGCCGGGGCC GTTGAGCTTC ACGACGGAGA TCCAGCGCTC GGCCACCAAG TCCTTGACTG CGTATTGGAC CGTCCGCAAA GAACGTCCGA TGAGCTTGGA AAGTGTCTTC TGGCTGACCA CCACGGCGTT CTGGTGGCCC ATCTGCGCCA CGAGGTGATG CAGCAGCATT GCCGCCGTGG GTTTCCTCGC AATAAGCCCG GCCCACGCCT CATGCGCTTT GCGTTCCGTT TGCACCCAGT GACCGGGCTT GTTCTTGGCT TGAATGCCGA TTTCTCTGGA CTGCGTGGCC ATGCTTATCT CCATGCGGTA GGGGTGCCGC ACGGTTGCGG CACCATGCGC AATCAGCTGC AACTTTTCGG CAGCGCGACA ACAATTATGC GTTGCGTAAA AGTGGCAGTC AATTACAGAT TTTCTTTAAC CTACGCAATG AGCTATTGCG GGGGGTGCCG CAATGAGCTG TTGCGTACCC CCCTTTTTTA AGTTGTTGAT TTTTAAGTCT TTCGCATTTC GCCCTATATC TAGTTCTTTG GTGCCCAAAG AAGGGCACCC CTGCGGGGTT CCCCCACGCC TTCGGCGCGG CTCCCCCTCC GGCAAAAAGT GGCCCCTCCG GGGCTTGTTG ATCGACTGCG CGGCCTTCGG CCTTGCCCAA GGTGGCGCTG CCCCCTTGGA ACCCCCGCAC TCGCCGCCGT GAGGCTCGGG GGGCAGGCGG GCGGGCTTCG CCCTTCGACT GCCCCCACTC GCATAGGCTT GGGTCGTTCC AGGCGCGTCA AGGCCAAGCC GCTGCGCGGT CGCTGCGCGA GCCTTGACCC GCCTTCCACT TGGTGTCCAA CCGGCAAGCG AAGCGCGCAG GCCGCAGGCC GGAGGCTTTT CCCCAGAGAA AATTAAAAAA ATTGATGGGG CAAGGCCGCA GGCCGCGCAG TTGGAGCCGG TGGGTATGTG GTCGAAGGCT GGGTAGCCGG TGGGCAATCC CTGTGGTCAA GCTCGTGGGC AGGCGCAGCC TGTCCATCAG CTTGTCCAGC AGGGTTGTCC ACGGGCCGAG CGAAGCGAGC CAGCCGGTGG CCGCTCGCGG CCATCGTCCA CATATCCACG GGCTGGCAAG GGAGCGCAGC GACCGCGCAG GGCGAAGCCC GGAGAGCAAG CCCGTAGGGG GGGCGCGCCC AGCTGTCTAG GGCGGCGGAT TTGTCCTACT CAGGAGAGCG TTCACCGACA AACAACAGAT AAAACGAAAG GCCCAGTCTT TCGACTGAGC CTTTCGTTTT ATTTGATGCC T
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