NM_080646.2;CAFS; CATCH22; CTHM; DGCR; DGS; DORV;TBX1C; TGA; VCF; VCFS;
100μg liquid plasmid(Instantly):$91
| name | pLV2-TRE3GS-EGFP-TBX1(human)-TetOne-Neo | another name | apLV2-TRE3GS-EGFP-TBX1(human)-TetOne-Neo |
| Insert Size (bp) | 1197bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression,Tetracycline induction | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | Green |
| Promoter | TRE3GS | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | pLV2-TRE3GS-EGFP-TetOne-Neo | 5′ sequencing primer | pEGFP-C-5' |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACTACTAAGT TTGTAGTACA TATTTAACAA ATACAATTTC TTTAAAAACT ACTAAGTTTG TAGTACATAT TTAACAAATA CAATTTCTTT AAAATGAAAA TAATTCAGAG GAATCACAGG TTTAGAGTAA ATGAAACCAC TAGGTAATTG GCAGTGGTAA TAGGGTATGG GGTGGGAAGT TTGGGATGAT TTTGGTTAGC TTGAGTTATC CAGTTGATCC AGACATGATA AGATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT GATCTCCCGA TCCGTCGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTCT AGCTAGAGGT CGACGGTAAA ACGCTAGCAA TGTCAAGGCC TCTCACTCTC TGATATTCAT TTCTTTGCAA GTTATAAATA CTGAATAATA AGATGACATG AACTACTACT GCTAGAGATT TTCCACACTG ACTAAAAGGG TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGGCA AAAAGCAGCT GCTTATATGC AGGATCTGAG GGCTCGCCAC TCCCCAGTCC CGCCCAGGCC ACGCCTCCCT GGAAAGTCCC CAGCGGAAAG TCCCTTGTAG CAAGCTCGAT ATCAGCAGTT CTTGAAGTAC TCCGGATGCA GCTCTCGGGC CACGTGATGA AATGCTAGGC GGCTGTCAAA CCTCCACTCT AACACTTCTC TCTCCGGGTC ATCCATCCCA TGCAGGCTCA CAGGGTGTAA CAAGCTGGTG TTCTCTCCTT TATTGGCCTC TTCTACCTTA TCTGGCTCAA CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA TCTGACCCCT GGCCCTGGTG TGTAGTTCTG CTAATCAGGG AAGTAGCCTT GTGTGTGGTA GATCCACAGA TCAAGGATAT CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCT CTTTTCTTTT AAAAAGTGGC TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG TACCTGAGGT GTGACTGGAA AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC AATCAGCATT GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC GACTGCAGAA TTAATTCCAG GCGGGGAGGC GGCCCAAAGG GAGATCCGAC TCGTCTGAGG GCGAAGGCGA AGACGCGGAA GAGGCCGCAG AGCCGGCAGC AGGCCGCGGG AAGGAAGGTC CGCTGGATTG AGGGCCGAAG GGACGTAGCA GAAGGACGTC CCGCGCAGAA TCCAGGTGGC AACACAGGCG AGCAGCCATG GAAAGGACGT CAGCTTCCCC GACAACACCA CGGAATTGTC AGTGCCCAAC AGCCGAGCCC CTGTCCAGCA GCGGGCAAGG CAGGCGGCGA TGAGTTCCGC CGTGGCAATA GGGAGGGGGA AAGCGAAAGT CCCGGAAAGG AGCTGACAGG TGGTGGCAAT GCCCCAACCA GTGGGGGTTG CGTCAGCAAA CACAGTGCAC ACCACGCCAC GTTGCCTGAC AACGGGCCAC AACTCCTCAT AAAGAGACAG CAACCAGGAT TTATACAAGG AGGAGAAAAT GAAAGCCATA CGGGAAGCAA TAGCATGATA CAAAGGCATT AAAGCAGCGT ATCCACATAG CGTAAAAGGA GCAACATAGT TAAGAATACC AGTCAATCTT TCACAAATTT TGTAATCCAG AGGTTGATTG TTCCAGACGC GTTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGG TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCGC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCCTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAGCCATGGT CGCGTTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCG CGCGTTTACC CGGGGAGCAT GTCAAGGTCA AAATCGTCAA GAGCGTCAGC AGGCAGCATA TCAAGGTCAA AGTCGTCAAG GGCATCGGCT GGGAGCATGT CTAAGTCAAA ATCGTCAAGG GCGTCGGTCG GCCCGCCGCT TTCGCACTTT AGCTGTTTCT CCAGGCCACA TATGATTAGT TCCAGGCCGA AAAGGAAGGC AGGTTCGGCT CCCTGCCGGT CGAACAGCTC AATTGCTTGT TTCAGAAGTG GGGGCATAGA ATCGGTGGTA GGTGTCTCTC TTTCCTCTTT TGCTACTTGA TGCTCCTGTT CCTCCAATAC GCAGCCCAGT GTAAAGTGGC CCACGGCGGA CAGAGCGTAC AGTGCGTTCT CCAGGGAGAA GCCTTGCTGA CACAGGAACG CGAGCTGATT TTCCAGGGTT TCGTACTGTT TCTCTGTTGG GCGGGTGCCG AGATGCACTT TAGCCCCGTC GCGATGTGAG AGGAGAGCAC AGCGGTATGA CTTGGCGTTG TTCCGCAGAA AGTCTTGCCA TGACTCGCCT TCCAGGGGGC AGGAGTGGGT ATGATGCCTG TCCAGCATCT CGATTGGCAG GGCATCGAGC AGGGCCCGCT TGTTCTTCAC GTGCCAGTAC AGGGTAGGCT GCTCAACTCC CAGCTTTTGA GCGAGTTTCC TTGTCGTCAG GCCTTCGATA CCGACTCCAT TGAGTAATTC CAGAGCAGAG TTTATGACTT TGCTCTTGTC CAGTCTAGAC ATGGTAATTC GATGATCCCC CTGGGGAGAG AGGTCGGTGA TTCGGTCAAC GAGGGAGCCG ACTGCCGACG TGCGCTCCGG AGGCTTGCAG AATGCGGAAC ACCGCGCGGG CAGGAACAGG GCCCACACTA CCGCCCCACA CCCCGCCTCC CGCACCGCCC CTTCCCGGCC GCTGCTCTCG GCGCGCCCTG CTGAGCAGCC GCTATTGGCC ACAGCCCATC GCGGTCGGCG CGCTGCCATT GCTCCCTGGC GCTGTCCGTC TGCGAGGGTA CTAGTGAGAC GTGCGGCTTC CGTTTGTCAC GTCCGGCACG CCGCGAACCG CAAGGAACCT TCCCGACTTA GGGGCGGAGC AGGAAGCGTC GCCGGGGGGC CCACAAGGGT AGCGGCGAAG ATCCGGGTGA CGCTGCGAAC GGACGTGAAG AATGTGCGAG ACCCAGGGTC GGCGCCGCTG CGTTTCCCGG AACCACGCCC AGAGCAGCCG CGTCCCTGCG CAAACCCAGG GCTGCCTTGG AAAAGGCGCA ACCCCAACCC CGTGGAATTA TCACCTCGAG TTTACTCCCT ATCAGTGATA GAGAACGTAT GAAGAGTTTA CTCCCTATCA GTGATAGAGA ACGTATGCAG ACTTTACTCC CTATCAGTGA TAGAGAACGT ATAAGGAGTT TACTCCCTAT CAGTGATAGA GAACGTATGA CCAGTTTACT CCCTATCAGT GATAGAGAAC GTATCTACAG TTTACTCCCT ATCAGTGATA GAGAACGTAT ATCCAGTTTA CTCCCTATCA GTGATAGAGA ACGTATAAGC TTTGCTTATG TAAACCAGGG CGCCTATAAA AGAGTGCTGA TTTTTTGAGT AAACTTCAAT TCCACAACAC TTTTGTCTTA TACCAACTTT CCGTACCACT TCCTACCCTC GTAAAGAATT CGCCACCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGGGTGGA GGCGGATCAG GAGGCGGTGG ATCTATGCAC TTCAGCACCG TCACCAGGGA CATGGAAGCC TTCACGGCCA GCAGCCTGAG CAGCCTGGGG GCCGCGGGGG GCTTCCCGGG CGCCGCGTCG CCCGGCGCCG ACCCGTACGG CCCGCGCGAG CCCCCGCCGC CGCCGCCGCG CTACGACCCG TGCGCCGCCG CCGCCCCCGG CGCCCCGGGC CCGCCGCCGC CGCCGCACGC CTACCCGTTT GCGCCGGCCG CCGGGGCCGC CACCAGCGCC GCCGCCGAGC CCGAGGGCCC CGGGGCCAGC TGCGCGGCCG CAGCCAAGGC GCCGGTGAAG AAGAACGCGA AGGTGGCCGG TGTGAGCGTG CAGCTAGAGA TGAAGGCGCT GTGGGACGAG TTCAACCAGC TGGGCACCGA GATGATCGTC ACCAAGGCCG GCAGGCGGAT GTTTCCCACC TTCCAAGTGA AGCTCTTCGG CATGGATCCC ATGGCCGACT ATATGCTGCT CATGGACTTC GTGCCGGTGG ACGATAAGCG CTACCGGTAC GCCTTCCACA GCTCCTCCTG GCTGGTGGCG GGGAAGGCCG ACCCTGCCAC GCCAGGCCGC GTGCACTACC ACCCGGACTC GCCTGCCAAG GGCGCGCAGT GGATGAAGCA AATCGTGTCC TTCGACAAGC TCAAGCTGAC CAACAACCTA CTGGACGACA ACGGCCACAT TATTCTGAAT TCCATGCACA GATACCAGCC CCGCTTCCAC GTGGTCTATG TGGACCCACG CAAAGATAGC GAGAAATATG CCGAGGAGAA CTTCAAAACC TTTGTGTTCG AGGAGACACG ATTCACCGCG GTCACTGCCT ACCAGAACCA TCGGATCACG CAGCTCAAGA TTGCCAGCAA TCCCTTCGCG AAAGGCTTCC GGGACTGTGA CCCTGAGGAC TGGCCCCGGA ACCACCGGCC CGGCGCACTG CCGCTCATGA GCGCCTTCGC GCGCTCGCGG AACCCCGTGG CTTCCCCGAC GCAGCCCAGC GGCACGGAGA AAGGTGGACA TGTCCTGAAG GACAAGGAAG TGAAAGCTGA GACGTCTAGG AACACACCAG AGAGAGAAGT GGAGCTTCTG AGGGATGCAG GTGGCTGTGT GAACCTGGGG CTCCCCTGCC CCGCAGAGTG CCAACCCTTC AATACCCAGG GCCTGGTGGC TGGGAGGACC GCAGGTGACC GTCTTTGTTG AGGATCCAGA CCACCTCCCC TGCGAGCTAA GCTGGACAGC CAATGACGGG TAAGAGAGTG ACATTTTTCA CTAACCTAAG ACAGGAGGGC CGTCAGAGCT ACTGCCTAAT CCAAAGACGG GTAAAAGTGA TAAAAATGTA TCACTCCAAC CTAAGACAGG CGCAGCTTCC GAGGGATTTG AGATCCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA ACCAAAACTA GAATGCAGTG AAAAAAATGC CTTATTTGTG AAATTTGTGA TGCTATTGCC TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAAGT CGATAAACTG GATCTCTGCT GTCCCTGTAA TAAACCCGAA AATTTTGAAT TTTTGTAATT TGTTTTTGTA GTTCTTTAGT TTGTATGTCT GTTGCTATTA TGTCTACTAT TCTTTCCCCT GCACTGTACC CCCCAATCCC CCCTTTTCTT TTAAAGGCGA TACCGTCGAG ATCCGTTCAC TAATCGAATG GATCTGTCTC TGTCTCTCTC TCCACCTTCT TCTTCTATTC CTTCGGGCCT GTCGGGTCCC CTCGGGGTTG GGAGGTGGGT CTGAAACGAT AATGGTGAAT ATCCCTGCCT AACTCTATTC ACTATAGAAA GTACAGCAAA AACTATTCTT AAACCTACCA AGCCTCCTAC TATCATTATG AATAATTTTA TATACCACAG CCAATTTGTT ATGTTAAACC AATTCCACAA ACTTGCCCAT TTATCTAATT CCAATAATTC TTGTTCATTC TTTTCTTGCT GGTTTTGCGA TTCTTCAATT AAGGAGTGTA TTAAGCTTGT GTAATTGTTA ATTTCTCTGT CCCACTCCAT CCAGGTCGTG TGATTCCAAA TCTGTTCCAG AGATTTATTA CTCCAACTAG CATTCCAAGG CACAGCAGTG GTGCAAATGA GTTTTCCAGA GCAACCCCAA ATCCCCAGGA GCTGTTGATC CTTTAGGTAT CTTTCCACAG CCAGGATTCT TGCCTGGAGC TGCTTGATGC CCCAGACTGT GAGTTGCAAC AGATGCTGTT GCGCCTCAAT AGCCCTCAGC AAATTGTTCT GCTGCTGCAC TATACCAGAC AATAATTGTC TGGCCTGTAC CGTCAGCGTC ATTGACGCTG CGCCCATAGT GCTTCCTGCT GCTCCCAAGA ACCCAAGGAA CAAAGCTCCT ATTCCCACTG CTCTTTTTTC TCTCTGCACC ACTCTTCTCT TTGCCTTGGT GGGTGCTACT CCTAATGGTT CAATTTTTAC TACTTTATAT TTATATAATT CACTTCTCCA ATTGTCCCTC ATATCTCCTC CTCCAGGTCT GAAGATCAGC GGCCGGCCGC TTGCTGTGCG GTGGTCTTAC TTTTGTTTTG CTCTTCCTCT ATCTTGTCTA AAGCTTCCTT GGTGTCTTTT ATCTCTATCC TTTGATGCAC ACAATAGAGG GTTGCTACTG TATTATATAA TGATCTAAGT TCTTCTGATC CTGTCTGAAG GGATGGTTGT AGCTGTCCCA GTATTTGTCT ACAGCCTTCT GATGTTTCTA ACAGGCCAGG ATTAACTGCG AATCGTTCTA GCTCCCTGCT TGCCCATACT ATATGTTTTA ATTTATATTT TTTCTTTCCC CCTGGCCTTA ACCGAATTTT TTCCCATCGC GATCTAATTC TCCCCCGCTT AATACTGACG CTCTCGCACC CATCTCTCTC CTTCTAGCCT CCGCTAGTCA AAATTTTTGG CGTACTCACC AGTCGCCGCC CCTCGCCTCT TGCCGTGCGC GCTTCAGCAA GCCGAGTCCT GCGTCGAGAG AGCTCCTCTG GTTTCCCTTT CGCTTTCAAG TCCCTGTTCG GGCGCCACTG CTAGAGATTT TCCACACTGA CTAAAAGGGT CTGAGGGATC TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC TTGAAGCACT CAAGGCAAGC TTTATTGAGG CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA GAGCTCCCAG GCTCAGATCT GGTCTAACCA GAGAGACCCA GTACAGGCAA AAAGCAGCTG CTTATATGCA GGATCTGAGG GCTCGCCACT CCCCAGTCCC GCCCAGGCCA CGCCTCCCTG GAAAGTCCCC AGCGGAAAGT CCCTTGTAGC AAGCTCGATA TCAGCAGTTC TTGAAGTACT CCGGATGCAG CTCTCGGGCC ACGTGATGAA ATGCTAGGCG GCTGTCAAAC CTCCACTCTA ACACTTCTCT CTCCGGGTCA TCCATCCCAT GCAGGCTCAC AGGGTGTAAC AAGCTGGTGT TCTCTCCTTT ATTGGCCTCT TCTACCTTAT CTGGCTCAAC TGGTACTAGC TTGTAGCACC ATCCAAAGGT CAGTGGATAT CTGACCCCTG GCCCTGGTGT GTAGTTCTGC TAATCAGGGA AGTAGCCTTG TGTGTGGTAG ATCCACAGAT CAAGGATATC TTGTCTTCTT TGGGAGTGAA TTAGCCCTTC CA
