pCMV-5UTR-GALC(human)-3×FLAG-3UTR-Neo
$70.00
In stock
SKU
PL22353
NM_001424076.1;
100μg liquid plasmid(Instantly):$91
| name | pCMV-5UTR-GALC(human)-3×FLAG-3UTR-Neo | another name | apCMV-5UTR-GALC(human)-3×FLAG-3UTR-Neo |
| Insert Size (bp) | 1422bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pCDNA3.1(+) | 5′ sequencing primer | |
| 3′ sequencing primer | BGH-R | Copy number | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC CGAGTGCTTA TTAACTTTTC TCTCAGTCCC TCACTCTGCT GCTCTTCTCG CCTCATCTCA CATAAGGGAA AACTCAGGCT CCAAAAGCAA AGTGATTCCA CCAAGATGAC AATGGAATCG GGACCTGGTG TCCAAGCCTG GTCCTCGCAC GAGGTAGATA CTCCCTGAGA TGCTGGGGAA GAGCCATGGT AGGGCCACTC ATGGCCCTCT TCCTTTGGCG GACCTTGGAA TCCACCTTCC CTGCGTTAAA GTGCTCCACC AGGTGACGCC GGAAGAGAAG CCAGCAGCAA CCTCCCGACT TCTAGTAAAT TACCCAGAGC CCTATCGTTC TCAGATATTG GATTATCTCT TTAAGCCGAA TTTTGGTGCC TCTTTGCATA TTTTAAAAGT GGAAATAGGT GGTGATGGGC AGACAACAGA CGGCACTGAG CCCTCCCACA TGCATTATGC ACTAGATGAG AATTATTTCC GAGGATACGA GTGGTGGTTG ATGAAAGAAG CTAAGAAGAG GAATCCCAAT ATTACACTCA TTGGGTTGCC ATGGTCATTC CCTGGATGGC TGGGAAAAGG TTTCGACTGG CCTTATGTCA ATCTTCAGCT GACTGCCTAT TATGTCGTGA CCTGGATTGT GGGCGCCAAG CGTTACCATG ATTTGGACAT TGATTATATT GGAATTTGGA ATGAGAGGTC ATATAATGCC AATTATATTA AGGTTTTTGG AGAATAGGTG GTATTTGGTT ACATGAATAT TAAGAAAAAT GCTGAATTAT CAAGGTCTCC AGCGAGTGAA AATCATAGCA AGTGATAATC TCTGGGAGTC CATCTCTGCA TCCATGCTCC TTGATGCCGA ACTCTTCAAG GTGGTTGATG TTATAGGGGC TCATTATCCT GGAACCCATT CAGCAAAAGA TGCAAAGTTG ACTGGGAAGA AGCTTTGGTC TTCTGAAGAC TTTAGCACTT TAAATAGTGA CATGGGTGCA GGCTGCTGGG GTCGCATTTT AAATCAGAAT TATATCAATG GCTATATGAC TTCCACAATC GCATGGAATT TAGTGGCTAG TTACTATGAA CAGTTGCCTT ATGGGAGATG CGGGTTGATG ACGGCCCAGG AGCCATGGAG TGGGCACTAC GTGGTAGAAT CTCCTGTCTG GGTATCAGCT CATACCACTC AGTTTACTCA ACCTGGCTGG TATTACCTGA AGACAGTTGG CCATTTAGAG AAAGGAGGAA GCTACGTAGC TCTGACTGAT GGCTTAGGGA ACCTCACCAT CATCATTGAA ACCATGAGTC ATAAACATTC TAAGTGCATA CGGCCATTTC TTCCTTATTT CAATGTGTCA CAACAATTTG CCACCTTTGT TCTTAAGGGA TCTTTTAGTG AAATACCAGA GCTACAGGTA TGGTATACCA AACTTGGAAA AACATCCGAA AGATTTCTTT TTAAGCAGCT GGATTCTCTA TGGCTCCTTG ACAGCGATGG CAGTTTCACA CTGAGCCTGC ATGAAGATGA GCTGTTCACA CTCACCACTC TCACCACTGG TCGCAAAGGC AGCTACCCGC TTCCTCCAAA ATCCCAGCCC TTCCCAAGTA CCTATAAGGA TGATTTCAAT GTTGATTACC CATTTTTTAG TGAAGCTCCA AACTTTGCTG ATCAAACTGG TGTATTTGAA TATTTTACAA ATATTGAAGA CCCTGGCGAG CATCACTTCA CGCTACGCCA AGTTCTCAAC CAGAGACCCA TTACATGGGC TGCCGATGCA TCCAACACAA TCAGTATTAT AGGAGACTAC AACTGGACCA ATCTGACTAT AAAGTGTGAT GTATACATAG AGACCCCTGA CACAGGAGGT GTGTTCATTG CAGGAAGAGT AAATAAAGGT GGTATTTTGA TTAGAAGTGC CAGAGGAATT TTCTTCTGGA TTTTTGCAAA TGGATCTTAC AGGGTTACAG GTGATTTAGC TGGATGGATT ATATATGCTT TAGGACGTGT TGAAGTTACA GCAAAAAAAT GGTATACACT CACGTTAACT ATTAAGGGTC ATTTCACCTC TGGCATGCTG AATGACAAGT CTCTGTGGAC AGACATCCCT GTGAATTTTC CAAAGAATGG CTGGGCTGCA ATTGGAACTC ACTCCTTTGA ATTTGCACAG TTTGACAACT TTCTTGTGGA AGCCACACGC GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT GACAAGTGAT ACTTAACAGG GCATCATAGA ATACTCTGGA TTTTCTTCCC TTCTTTTTGG TTTTGGTTCA GAGCCAATTC TTGTTTCATT GGAACAGTAT ATGAGGCTTT TGAGACTAAA AATAATGAAG AGTAAAAGGG GAGAGAAATT TATTTTTAAT TTACCCTGTG GAAGATTTTA TTAGAATTAA TTCCAAGGGG AAAACTGGTG AATCTTTAAC ATTACCTGGT GTGTTCCCTA ACATTCAAAC TGTGCATTGG CCATACCCTT AGGAGTGGTT TGAGTAGTAC AGACCTCGAA GCCTTGCTGC TAACACTGAG GTAGCTCTCT TCATCTTATT TGCAAGCGGT CCTGTAGATG GCAGTAACTT GATCATCACT GAGATGTATT TATGCATGCT GACCGTGTGT CCAAGTGAGC CAGTGTCTTC ATCACAAGAT GATGCTGCCA TAATAGAAAG CTGAAGAACA CTAGAAGTAG CTTTTTGAAA ACCACTTCAA CCTGTTATGC TTTATGCTCT AAAAAGTATT TTTTTATTTT CCTTTTTAAG ATGATACTTT TGAAATGCAG GATATGATGA GTGGGATGAT TTTAAAAATG CCTCTTTAAT AAACTACCTC TAACACTATT TCTGCAGTAA TAGATATTAG CAGATTAATT GGGTTATTTG CATTATTTAA TTTTTTTGAT TCCAAGTTTT GGTCTTGTAA CCACTATAAC TCTCTGTGAA CGTTTTTCCA GGTGGCTGGA AGAAGGAAGA AAACCTGATA TAGCCAATGC TGTTGTAGTC GTTTCCTCAG CCTCATCTCA CTGTGCTGTG GTCTGTCCTC ACATGTGCAC TGGTAACAGA CTCACACAGC TGATGAATGC TTTTCTCTCC TTATGTGTGG AAGGAGGGGA GCACTTAGAC ATTTGCTAAC TCCCAGAATT GGATCATCTC CTAAGATGTA CTTACTTTTT AAAGTCCAAA TATGTTTATA TTTAAATATA CGTGAGCGTG TTCATCATGT TGTATGATTT ATACTAAGCA TTAATGTGGC TCTATGTAGC AAATCAGTTA TTCATGTAGG TAAAGTAAAT CTAGAATTAT TTATAAGAAT TACTCATTGA ACTAATTCTA CTATTTAGGA ATTTGTAAGA GTCTAACATA GGCTTAGCTA CAGTGAAGTT TTGCATTGCT TTTGAAGACA AGAAGATAAG TGCTAGAATA AATAAGATTA CAGAGAAAAT TTTTTGTTAA AACCAAGTGA TTTCCAGCTG ATGTATCTAA TATTTTTTAA AACGAACATT ATAGAGGTGT AATTTATTTA CAATAAAATG TTCCTACTTT AAATATACAA TTCAGTGAGT TTTGATAAAT TGATATACCC ATGTAACCAA CACTCCAGTC AAGCTTCAGA ATATTTCCAT CACCCCAGAA GGTTCTCTTG TATACCTGCT CAGTCAGTTC CTTTCACTCC CAATTGTTGG CAGCCATTGA TAGGAATTCT ATCACTATAG GTTAGTTTTC TTTGTTCCAG AACATCATGA AAGCGGCGTC ATGTACTGTG TATTCTTATG AATGGTTTCT TTCCATCAGC ATAATGATTT GAGATTTGTC CATGTTGTGT GATTCAGTGG TTTGTTCCTT CTTATTTCTG AAGAGTTTTC CATTGTATGA ATATACCACA ATTTGTTTCC TCCCCACCAG TTTCTGATAC TACAATTAAA ACTGTCTACA TTTACAACTC GAGTCTAGAG GGCCCGTTTA AACCCGCTGA TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC CCCCGTGCCT TCCTTGACCC TGGAAGGTGC CACTCCCACT GTCCTTTCCT AATAAAATGA GGAAATTGCA TCGCATTGTC TGAGTAGGTG TCATTCTATT CTGGGGGGTG GGGTGGGGCA GGACAGCAAG GGGGAGGATT GGGAAGACAA TAGCAGGCAT GCTGGGGATG CGGTGGGCTC TATGGCTTCT GAGGCGGAAA GAACCAGCTG GGGCTCTAGG GGGTATCCCC ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTA ATTCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CTGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CGACGGATCG GGAGATCTCC CGATCCCCTA TGGT
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