pCMV-EGFP-APAF1(human)-Neo
$99.00
In stock
SKU
PL22197
NM_181861.2;APAF-1; CED4
100μg liquid plasmid(Instantly):$142
| name | pCMV-EGFP-APAF1(human)-Neo | another name | apCMV-EGFP-APAF1(human)-Neo |
| Insert Size (bp) | 3947bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pEGFP-C1 | 5′ sequencing primer | pEGFP-C-5' |
| 3′ sequencing primer | Copy number | Low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGG GCGGCGGAGG GAGTGGAGGA GGGGGAAGCC TCGAGATGGA TGCAAAAGCT CGAAATTGTT TGCTTCAACA TAGAGAAGCT CTGGAAAAGG ACATCAAGAC ATCCTACATC ATGGATCACA TGATTAGTGA TGGATTTTTA ACAATATCAG AAGAGGAAAA AGTAAGAAAT GAGCCCACTC AACAGCAAAG AGCAGCTATG CTGATTAAAA TGATACTTAA AAAAGATAAT GATTCCTACG TATCATTCTA CAATGCTCTA CTACATGAAG GATATAAAGA TCTTGCTGCC CTTCTCCATG ATGGCATTCC TGTTGTCTCT TCTTCCAGTG GTAAAGATTC AGTTAGTGGA ATAACTTCGT ATGTAAGGAC AGTCCTGTGT GAAGGTGGAG TACCACAGAG GCCAGTTGTT TTTGTCACAA GGAAGAAGCT GGTGAATGCA ATTCAGCAGA AGCTCTCCAA ATTGAAAGGT GAACCAGGAT GGGTCACCAT ACATGGAATG GCAGGCTGTG GGAAGTCTGT ATTAGCTGCA GAAGCTGTTA GAGATCATTC CCTTTTAGAA GGTTGTTTCC CAGGGGGAGT GCATTGGGTT TCAGTTGGGA AACAAGACAA ATCTGGGCTT CTGATGAAAC TGCAGAATCT TTGCACACGG TTGGATCAGG ATGAGAGTTT TTCCCAGAGG CTTCCACTTA ATATTGAAGA GGCTAAAGAC CGTCTCCGCA TTCTGATGCT TCGCAAACAC CCAAGGTCTC TCTTGATCTT GGATGATGTT TGGGACTCTT GGGTGTTGAA AGCTTTTGAC AGTCAGTGTC AGATTCTTCT TACAACCAGA GACAAGAGTG TTACAGATTC AGTAATGGGT CCTAAATATG TAGTCCCTGT GGAGAGTTCC TTAGGAAAGG AAAAAGGACT TGAAATTTTA TCCCTTTTTG TTAATATGAA GAAGGCAGAT TTGCCAGAAC AAGCTCATAG TATTATAAAA GAATGTAAAG GCTCTCCCCT TGTAGTATCT TTAATTGGTG CACTTTTACG TGATTTTCCC AATCGCTGGG AGTACTACCT CAAACAGCTT CAGAATAAGC AGTTTAAGAG AATAAGGAAA TCTTCGTCTT ATGATTATGA GGCTCTAGAT GAAGCCATGT CTATAAGTGT TGAAATGCTC AGAGAAGACA TCAAAGATTA TTACACAGAT CTTTCCATCC TTCAGAAGGA CGTTAAGGTG CCTACAAAGG TGTTATGTAT TCTCTGGGAC ATGGAAACTG AAGAAGTTGA AGACATACTG CAGGAGTTTG TAAATAAGTC TCTTTTATTC TGTGATCGGA ATGGAAAGTC GTTTCGTTAT TATTTACATG ATCTTCAAGT AGATTTTCTT ACAGAGAAGA ATTGCAGCCA GCTTCAGGAT CTACATAAGA AGATAATCAC TCAGTTTCAG AGATATCACC AGCCGCATAC TCTTTCACCA GATCAGGAAG ACTGTATGTA TTGGTACAAC TTTCTGGCCT ATCACATGGC CAGTGCCAAG ATGCACAAGG AACTTTGTGC TTTAATGTTT TCCCTGGATT GGATTAAAGC AAAAACAGAA CTTGTAGGCC CTGCTCATCT GATTCATGAA TTTGTGGAAT ACAGACATAT ACTAGATGAA AAGGATTGTG CAGTCAGTGA GAATTTTCAG GAGTTTTTAT CTTTAAATGG ACACCTTCTT GGACGACAGC CATTTCCTAA TATTGTACAA CTGGGTCTCT GTGAGCCGGA AACTTCAGAA GTTTATCAGC AAGCTAAGCT GCAGGCCAAG CAGGAGGTCG ATAATGGAAT GCTTTACCTG GAATGGATAA ACAAAAAAAA CATCACGAAT CTTTCCCGCT TAGTTGTCCG CCCCCACACA GATGCTGTTT ACCATGCCTG CTTTTCTGAG GATGGTCAGA GAATAGCTTC TTGTGGAGCT GATAAAACCT TACAGGTGTT CAAAGCTGAA ACAGGAGAGA AACTTCTAGA AATCAAGGCT CATGAGGATG AAGTGCTTTG TTGTGCATTC TCTACAGATG ACAGATTTAT AGCAACCTGC TCAGTGGATA AAAAAGTGAA GATTTGGAAT TCTATGACTG GGGAACTAGT ACACACCTAT GATGAGCACT CAGAGCAAGT CAATTGCTGC CATTTCACCA ACAGTAGTCA TCATCTTCTC TTAGCCACTG GGTCAAGTGA CTGCTTCCTC AAACTTTGGG ATTTGAATCA AAAAGAATGT CGAAATACCA TGTTTGGTCA TACAAATTCA GTCAATCACT GCAGATTTTC ACCAGATGAT AAGCTTTTGG CTAGTTGTTC AGCTGATGGA ACCTTAAAGC TTTGGGATGC GACATCAGCA AATGAGAGGA AAAGCATTAA TGTGAAACAG TTCTTCCTAA ATTTGGAGGA CCCTCAAGAG GATATGGAAG TGATAGTGAA GTGTTGTTCG TGGTCTGCTG ATGGTGCAAG GATAATGGTG GCAGCAAAAA ATAAAATCTT TCTTTTTGAC ATTCATACTA GTGGCCTATT GGGAGAAATC CACACGGGCC ATCACAGCAC CATCCAGTAC TGTGACTTCT CCCCACAAAA CCATTTGGCA GTGGTTGCTT TGTCCCAGTA CTGTGTAGAG TTGTGGAATA CAGACTCACG TTCAAAGGTG GCTGATTGCA GAGGACATTT AAGTTGGGTT CATGGTGTGA TGTTTTCTCC TGATGGATCA TCATTTTTGA CATCTTCTGA TGACCAGACA ATCAGGCTCT GGGAGACAAA GAAAGTATGT AAGAACTCTG CTGTAATGTT AAAGCAAGAA GTAGATGTTG TGTTTCAAGA AAATGAAGTG ATGGTCCTTG CAGTTGACCA TATAAGACGT CTGCAACTCA TTAATGGAAG AACAGGTCAG ATTGATTATC TGACTGAAGC TCAAGTTAGC TGCTGTTGCT TAAGTCCACA TCTTCAGTAC ATTGCATTTG GAGATGAAAA TGGAGCCATT GAGATTTTAG AACTTGTAAA CAATAGAATC TTCCAGTCCA GGTTTCAGCA CAAGAAAACT GTATGGCACA TCCAGTTCAC AGCCGATGAG AAGACTCTTA TTTCAAGTTC TGATGATGCT GAAATTCAGG TATGGAATTG GCAATTGGAC AAATGTATCT TTCTACGAGG CCATCAGGAA ACAGTGAAAG ACTTTAGACT CTTGAAAAAT TCAAGACTGC TTTCTTGGTC ATTTGATGGA ACAGTGAAGG TATGGAATAT TATTACTGGA AATAAAGAAA AAGACTTTGT CTGTCACCAG GGTACAGTAC TTTCTTGTGA CATTTCTCAC GATGCTACCA AGTTTTCATC TACCTCTGCT GACAAGACTG CAAAGATCTG GAGTTTTGAT CTCCTTTTGC CACTTCATGA ATTGAGGGGC CACAACGGCT GTGTGCGCTG CTCTGCCTTC TCTGTGGACA GTACCCTGCT GGCAACGGGA GATGACAATG GAGAAATCAG GATATGGAAT GTCTCAAACG GTGAGCTTCT TCATTTGTGT GCTCCGCTTT CAGAAGAAGG AGCTGCTACC CATGGAGGCT GGGTGACTGA CCTTTGCTTT TCTCCAGATG GCAAAATGCT TATCTCTGCT GGAGGATATA TTAAGTGGTG GAACGTTGTC ACTGGGGAAT CCTCACAGAC CTTCTACACA AATGGAACCA ATCTTAAGAA AATACACGTG TCCCCTGACT TCAAAACATA TGTGACTGTG GATAATCTTG GTATTTTATA TATTTTACAG ACTTTAGAAT AAGGATCCAC CGGATCTAGA TAACTGATCA TAATCAGCCA TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTAACGCGTA AATTGTAAGC GTTAATATTT TGTTAAAATT CGCGTTAAAT TTTTGTTAAA TCAGCTCATT TTTTAACCAA TAGGCCGAAA TCGGCAAAAT CCCTTATAAA TCAAAAGAAT AGACCGAGAT AGGGTTGAGT GTTGTTCCAG TTTGGAACAA GAGTCCACTA TTAAAGAACG TGGACTCCAA CGTCAAAGGG CGAAAAACCG TCTATCAGGG CGATGGCCCA CTACGTGAAC CATCACCCTA ATCAAGTTTT TTGGGGTCGA GGTGCCGTAA AGCACTAAAT CGGAACCCTA AAGGGAGCCC CCGATTTAGA GCTTGACGGG GAAAGCCGGC GAACGTGGCG AGAAAGGAAG GGAAGAAAGC GAAAGGAGCG GGCGCTAGGG CGCTGGCAAG TGTAGCGGTC ACGCTGCGCG TAACCACCAC ACCCGCCGCG CTTAATGCGC CGCTACAGGG CGCGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTCCTG AGGCGGAAAG AACCAGCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAGATCG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCTAGG GGGAGGCTAA CTGAAACACG GAAGGAGACA ATACCGGAAG GAACCCGCGC TATGACGGCA ATAAAAAGAC AGAATAAAAC GCACGGTGTT GGGTCGTTTG TTCATAAACG CGGGGTTCGG TCCCAGGGCT GGCACTCTGT CGATACCCCA CCGAGACCCC ATTGGGGCCA ATACGCCCGC GTTTCTTCCT TTTCCCCACC CCACCCCCCA AGTTCGGGTG AAGGCCCAGG GCTCGCAGCC AACGTCGGGG CGGCAGGCCC TGCCATAGCC TCAGGTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCAT GCAT
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