37825-sacas9（repeat-opt）
$70.00
In stock
SKU
PL22160
-
100μg liquid plasmid(Instantly):$113
| name | 37825-sacas9（repeat-opt） | another name | a37825-sacas9（repeat-opt） |
| Insert Size (bp) | Plasmid host | Mammary cell,Adeno-associated virus | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Golden Staphylococcus | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | pAAV-CAG-GFP | 5′ sequencing primer | |
| 3′ sequencing primer | WPRE-R | Copy number | Low |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TAAATTGTAA GCGTTAATAT TTTGTTAAAA TTCGCGTTAA ATTTTTGTTA AATCAGCTCA TTTTTTAACC AATAGGCCGA AATCGGCAAA ATCCCTTATA AATCAAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC AAGAGTCCAC TATTAAAGAA CGTGGACTCC AACGTCAAAG GGCGAAAAAC CGTCTATCAG GGCGATGGCC CACTACGTGA ACCATCACCC TAATCAAGTT TTTTGGGGTC GAGGTGCCGT AAAGCACTAA ATCGGAACCC TAAAGGGAGC CCCCGATTTA GAGCTTGACG GGGAAAGCCG GCGAACGTGG CGAGAAAGGA AGGGAAGAAA GCGAAAGGAG CGGGCGCTAG GGCGCTGGCA AGTGTAGCGG TCACGCTGCG CGTAACCACC ACACCCGCCG CGCTTAATGC GCCGCTACAG GGCGCGTCCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GCGCGCTCGC TCGCTCACTG AGGCCGCCCG GGCAAAGCCC GGGCGTCGGG CGACCTTTGG TCGCCCGGCC TCAGTGAGCG AGCGAGCGCG CAGAGAGGGA GTGGCCAACT CCATCACTAG GGGTTCCTTG TAGTTAATGA TTAACCCGCC ATGCTACTTA TCTACGTAGC CATGCTCTAG GAAGAGTACC ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTC GAGGTGAGCC CCACGTTCTG CTTCACTCTC CCCATCTCCC CCCCCTCCCC ACCCCCAATT TTGTATTTAT TTATTTTTTA ATTATTTTGT GCAGCGATGG GGGCGGGGGG GGGGGGGGGG sGCGCGCCGG GGGGGGGGGG GGGGGGGGGG GGGGGGGGsG GGGsGrGGCG GAGAGGTGCG GCGGCAGCCA ATCAGAGCGG CGCGCTCCGA AAGTTTCCTT TTATGGCGAG GCGGCGGCGG CGGCGGCCCT ATAAAAAGCG AAGCGCGCGG CGGGCGGGAG TCGCTGCGCG CTGCCTTCGC CCCGTGCCCC GCTCCGCCGC CGCCTCGCGC CGCCCGCCCC GGCTCTGACT GACCGCGTTA CTCCCACAGG TGAGCGGGCG GGACGGCCCT TCTCCTCCGG GCTGTAATTA GCGCTTGGTT TAATGACGGC TTGTTTCTTT TCTGTGGCTG CGTGAAAGCC TTGAGGGGCT CCGGGAGCCA CCATGGCCCC AAAGAAGAAG CGGAAGGTCG GTATCCACGG AGTCCCAGCA GCCAAGCGGA ACTACATCCT GGGCCTGGAC ATCGGCATCA CCAGCGTGGG CTACGGCATC ATCGACTACG AGACACGGGA CGTGATCGAT GCCGGCGTGC GGCTGTTCAA AGAGGCCAAC GTGGAAAACA ACGAGGGCAG GCGGAGCAAG AGAGGCGCCA GAAGGCTGAA GCGGCGGAGG CGGCATAGAA TCCAGAGAGT GAAGAAGCTG CTGTTCGACT ACAACCTGCT GACCGACCAC AGCGAGCTGA GCGGCATCAA CCCCTACGAG GCCAGAGTGA AGGGCCTGAG CCAGAAGCTG AGCGAGGAAG AGTTCTCTGC CGCCCTGCTG CACCTGGCCA AGAGAAGAGG CGTGCACAAC GTGAACGAGG TGGAAGAGGA CACCGGCAAC GAGCTGTCCA CCAAAGAGCA GATCAGCCGG AACAGCAAGG CCCTGGAAGA GAAATACGTG GCCGAACTGC AGCTGGAACG GCTGAAGAAA GACGGCGAAG TGCGGGGCAG CATCAACAGA TTCAAGACCA GCGACTACGT GAAAGAAGCC AAACAGCTGC TGAAGGTGCA GAAGGCCTAC CACCAGCTGG ACCAGAGCTT CATCGACACC TACATCGACC TGCTGGAAAC CCGGCGGACC TACTATGAGG GACCTGGCGA GGGCAGCCCC TTCGGCTGGA AGGACATCAA AGAATGGTAC GAGATGCTGA TGGGCCACTG CACCTACTTC CCCGAGGAAC TGCGGAGCGT GAAGTACGCC TACAACGCCG ACCTGTACAA CGCCCTGAAC GACCTGAACA ATCTCGTGAT CACCAGGGAC GAGAACGAGA AGCTGGAATA TTACGAGAAG TTCCAGATCA TCGAGAACGT GTTCAAGCAG AAGAAGAAGC CCACCCTGAA GCAGATCGCC AAAGAAATCC TCGTGAACGA AGAGGATATT AAGGGCTACA GAGTGACCAG CACCGGCAAG CCCGAGTTCA CCAACCTGAA GGTGTACCAC GACATCAAGG ACATTACCGC CCGGAAAGAG ATTATTGAGA ACGCCGAGCT GCTGGATCAG ATTGCCAAGA TCCTGACCAT CTACCAGAGC AGCGAGGACA TCCAGGAAGA ACTGACCAAT CTGAACTCCG AGCTGACCCA GGAAGAGATC GAGCAGATCT CTAATCTGAA GGGCTATACC GGCACCCACA ACCTGAGCCT GAAGGCCATC AACCTGATCC TGGACGAGCT GTGGCACACC AACGACAACC AGATCGCTAT CTTCAACCGG CTGAAGCTGG TGCCCAAGAA GGTGGACCTG TCCCAGCAGA AAGAGATCCC CACCACCCTG GTGGACGACT TCATCCTGAG CCCCGTCGTG AAGAGAAGCT TCATCCAGAG CATCAAAGTG ATCAACGCCA TCATCAAGAA GTACGGCCTG CCCAACGACA TCATTATCGA GCTGGCCCGC GAGAAGAACT CCAAGGACGC CCAGAAAATG ATCAACGAGA TGCAGAAGCG GAACCGGCAG ACCAACGAGC GGATCGAGGA AATCATCCGG ACCACCGGCA AAGAGAACGC CAAGTACCTG ATCGAGAAGA TCAAGCTGCA CGACATGCAG GAAGGCAAGT GCCTGTACAG CCTGGAAGCC ATCCCTCTGG AAGATCTGCT GAACAACCCC TTCAACTATG AGGTGGACCA CATCATCCCC AGAAGCGTGT CCTTCGACAA CAGCTTCAAC AACAAGGTGC TCGTGAAGCA GGAAGAAAAC AGCAAGAAGG GCAACCGGAC CCCATTCCAG TACCTGAGCA GCAGCGACAG CAAGATCAGC TACGAAACCT TCAAGAAGCA CATCCTGAAT CTGGCCAAGG GCAAGGGCAG AATCAGCAAG ACCAAGAAAG AGTATCTGCT GGAAGAACGG GACATCAACA GGTTCTCCGT GCAGAAAGAC TTCATCAACC GGAACCTGGT GGATACCAGA TACGCCACCA GAGGCCTGAT GAACCTGCTG CGGAGCTACT TCAGAGTGAA CAACCTGGAC GTGAAAGTGA AGTCCATCAA TGGCGGCTTC ACCAGCTTTC TGCGGCGGAA GTGGAAGTTT AAGAAAGAGC GGAACAAGGG GTACAAGCAC CACGCCGAGG ACGCCCTGAT CATTGCCAAC GCCGATTTCA TCTTCAAAGA GTGGAAGAAA CTGGACAAGG CCAAAAAAGT GATGGAAAAC CAGATGTTCG AGGAAAAGCA GGCCGAGAGC ATGCCCGAGA TCGAAACCGA GCAGGAGTAC AAAGAGATCT TCATCACCCC CCACCAGATC AAGCACATTA AGGACTTCAA GGACTACAAG TACAGCCACC GGGTGGACAA GAAGCCTAAT AGAGAGCTGA TTAACGACAC CCTGTACTCC ACCCGGAAGG ACGACAAGGG CAACACCCTG ATCGTGAACA ATCTGAACGG CCTGTACGAC AAGGACAATG ACAAGCTGAA AAAGCTGATC AACAAGAGCC CCGAAAAGCT GCTGATGTAC CACCACGACC CCCAGACCTA CCAGAAACTG AAGCTGATTA TGGAACAGTA CGGCGACGAG AAGAATCCCC TGTACAAGTA CTACGAGGAA ACCGGGAACT ACCTGACCAA GTACTCCAAA AAGGACAACG GCCCCGTGAT CAAGAAGATT AAGTATTACG GCAACAAACT GAACGCCCAT CTGGACATCA CCGACGACTA CCCCAACAGC AGAAACAAGG TCGTGAAGCT GTCCCTGAAG CCCTACAGAT TCGACGTGTA CCTGGACAAT GGCGTGTACA AGTTCGTGAC CGTGAAGAAT CTGGATGTGA TCAAAAAAGA AAACTACTAC GAAGTGAATA GCAAGTGCTA TGAGGAAGCT AAGAAGCTGA AGAAGATCAG CAACCAGGCC GAGTTTATCG CCTCCTTCTA CAACAACGAT CTGATCAAGA TCAACGGCGA GCTGTATAGA GTGATCGGCG TGAACAACGA CCTGCTGAAC CGGATCGAAG TGAACATGAT CGACATCACC TACCGCGAGT ACCTGGAAAA CATGAACGAC AAGAGGCCCC CCAGGATCAT TAAGACAATC GCCTCCAAGA CCCAGAGCAT TAAGAAGTAC AGCACAGACA TTCTGGGCAA CCTGTATGAA GTGAAATCTA AGAAGCACCC TCAGATCATC AAAAAGGGCA AAAGGCCGGC GGCCACGAAA AAGGCCGGCC AGGCAAAAAA GAAAAAGGGA TCCTATCCTT ACGACGTTCC TGATTACGCC TATCCGTACG ATGTTCCTGA CTACGCATAC CCTTACGATG TACCAGACTA CGCGTAAATC GATAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAAT CATCGTCCTT TCCTTGGCTG CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCATCGATAC CGTCGACCCG GGCGGCCGCT TCGAGCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA GATGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTAAAA TCGATAAGGA TCTTCCTAGA GCATGGCTAC GTAGATAAGT AGCATGGCGG GTTAATCATT AACTACAAGG AACCCCTAGT GATGGAGTTG GCCACTCCCT CTCTGCGCGC TCGCTCGCTC ACTGAGGCCG GGCGACCAAA GGTCGCCCGA CGCCCGGGCT TTGCCCGGGC GGCCTCAGTG AGCGAGCGAG CGCGCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CT
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