pJET1.2-EVM106(virus)
$70.00
In stock
SKU
PL22085
NC_004105.1
100μg liquid plasmid(Instantly):$91
| name | pJET1.2-EVM106(virus) | another name | apJET1.2-EVM106(virus) |
| Insert Size (bp) | 1935bp | Plasmid host | Escherichia coli |
| Plasmid uses | Gene template | Fragment type | cDNA |
| Fragment species | Virus | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | T7 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pJET1.2-Stuffer | 5′ sequencing primer | Pjet1-F1 |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCCCCTGCAG CCGAATTATA TTATTTTTGC CAAATAATTT TTAACAAAAG CTCTGAAGTC TTCTTCATTT AAATTCTTAG ATGATACTTC ATCTGGAAAA TTGTCCCAAT TAGTAGCATC ACGCTGTGAG TAAGTTCTAA ACCATTTTTT TATTGTTGTA TTATCTCTAA TCTTACTACT CGATGAGTTT TCGGTATTAT CTCTATTTTT AACTTGGAGC AGGTTCCATT CATTGTTTTT TTCATCATAG TGAATAAAAT CAACTGCTTT AACACTTGTG CCTGAACACC ATATCCATCC GGCGTAATAC GACTCACTAT AGGGAGAGCG GCCGCCAGAT CTTCCGGATG GCTCGAGATG GAAGCCGTGC TCAATAGTGA TGGGTTTTTA ACATCTAACG CAGGACTAAA ATCTAGTTAT ATTAATCAAA CTCTTTCTTT GGTAGATGAA GATCATATAC ACACTTCTGA TAAATCTTTG TCTTGTAGTG TATGCAATTC ATTATCCCAG ATTGTAGACG ATGACTTTAT ATCCGCAGGA GCTAGAAATC AACGTACCAA ACCTAAACGT GCAGGAAATA ATCAATCTCA ACAGCCTATC AAAAAGGATT GTATGGTTTC CATCGACGAA GTAGCATCCA CGCATGATTG GAGTACGAGA TTGAGAAATG ATGGGAATGC AATTGCTAAA TATCTAACTA CTAACAAGTA TGACACATCT AACTTTACTA TTCAGGATAT GCTTAACATT ATGAATAAAC TAAATATTGT CAGAACAAAT AGAAACGAGC TATTTCAACT CCTTACCCAT GTAAAGAGCA CGTTGAACAA TGCTAGTGTT TCTGTGAAAT GTACTCATCC TTTAGTACTT ATTCATTCTC GAGCTAGTCC TAGAATCGGT GACCAACTCA AAGAGTTAGA TAAAATATAC TCTCCATCTA ATCATCATAT TCTTCTGTCG ACTACACGAT TCCAATCCAT GCATTTTACC GATATGTCTA GTTCACAAGA TTTGTCTTTT ATTTATAGAA AACCAGAAAC TAATTACTAC ATTCATCCTA TTCTGATGGC ACTATTCGGT ATTAAACTTC CAGCGCTCGA GAACGCGTAT GTACATGGAG ACACATATAG TCTAATCCAG CAACTTTATG AATTTAGAAG AGTAAAGTCT TATAATTATA TGTTGTTGGT TAATCGTCTT ACGGAGGATA ATCCGATAGT GATTACAGGT GTATCAGATC TAATTTCCAC AGAGATTCAG AGAGCAAACA TGCATACCAT GATTAGAAAA GCAATTATGA ACATTAGAAT GGGAATTTTT TATTGTAACG ATGATGATGC GGTAGATCCC CATCTAATGA AGATTATTCA TACTGGATGC TCTCAAGTTA TGACCGATGA GGAACAGATA TTAGCTTCTA TTTTGTCTAT AGTTGGATTT AGACCTACGT TGGTTTCTGT GGCTAGACCT ATGAACGGCA TCAGTTACGA TATGAAACTT CAGGCGGCGC CATACATCGT TGTTAATCCT ATGAAGATGA TCACAACATC CGACAGTCCG ATTTCTATCA ATTCCAAGGA TATTTATTCT ATGGCATTCG ATGGCAATAG TGGAAGAGTG GTGTTCGCTC CTCCTAACAT AGGCTATGGA AGATGTTCTG GAGTTACACA CATTGATCCA TTGGGAACTA ATGTGATGGG TAGTGCTGTT CATTCCCCTG TTATCGTTAA TGGAGCAATG ATGTTTTATG TAGAACGACG CCAGAATAAG AATATGTTTG GTGGAGAATG TTACACCGGC TTTAGATCTC TAATAGATGA TACTCCAATT GACGTATCGC CAGAAATCAT GCTAAACGGT ATCATGTATA GGTTAAAGTC CGCAGTCTGT TATAAACTCG GAGACCAATT CTTTGATTGT GGATCGTCTG ATATCTTCTT GAAGGGACAT TATACGATTC TATTTACAGA AAATGGACCC TGGATGTACG ATCCTCTTTC TGTTTTCAAT CCGGGAGCTA GAAATGCTAG ATTGATGAGA GCTCTCAAAA ACCAGTACAA GAAATTATCA ATGGATTCAG ACGATGGTTT TTATGAATGG TTGAATGGCG ATGGTTCAGT ATTTGCTGCC TCAAAACAGC AAATGTTGAT GAATCACGTT GCTAACTTTG ACGACGATCT TCTAACTATG GAAGAAGCCA TGTCGATGAT TTCAAGACAT TGTTGTATCT TAATTTATGC ACAGGATTAT GATCAATATA TTAGCGCTAG GCATATTACA GAACTATTTT AGTCTAGAAG ATCTCCTACA ATATTCTCAG CTGCCATGGA AAATCGATGT TCTTCTTTTA TTCTCTCAAG ATTTTCAGGC TGTATATTAA AACTTATATT AAGAACTATG CTAACCACCT CATCAGGAAC CGTTGTAGGT GGCGTGGGTT TTCTTGGCAA TCGACTCTCA TGAAAACTAC GAGCTAAATA TTCAATATGT TCCTCTTGAC CAACTTTATT CTGCATTTTT TTTGAACGAG GTTTAGAGCA AGCTTCAGGA AACTGAGACA GGAATTTTAT TAAAAATTTA AATTTTGAAG AAAGTTCAGG GTTAATAGCA TCCATTTTTT GCTTTGCAAG TTCCTCAGCA TTCTTAACAA AAGACGTCTC TTTTGACATG TTTAAAGTTT AAACCTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACATTATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCC
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