pACSL4(human)-luc2
$84.00
In stock
SKU
PL22036
NC_000023.11
100μg liquid plasmid(Instantly):$105
| name | pACSL4(human)-luc2 | another name | apACSL4(human)-luc2 |
| Insert Size (bp) | 2201bp | Plasmid host | Mammary cell |
| Plasmid uses | Promoter detection | Fragment type | Promoter |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Fluc | |
| Promoter | ACSL4 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pGL4.10[luc2] | 5′ sequencing primer | RVP3 |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGCCTAACTG GCCGGTACCA GTTAAATGTG GAAGAAGGTG AGATGTGGTA GGTGATGAAG ACAGCCAGAA CTGTACACAC TAATGAATAG CTTAAGTAAT AAGAGATCTT GAAAATCCAA GTAGTTTCTT GGTCTGATAA ACATATCTAT ATGTATATCA TACACACATT TGTATACCAA AAAACGGATA GTTTCAGGCT ATAGTGATTT CTTTCCTTTA TATCTTGTAG AGCTGGAGGA ACTTCAGTAA GCTGAAGACT CTGTTCAGAG GAAATTACCT AATTGAAAAT GTCTGGGCTA AGAGACCGAC AATAAAGGAA ACTGGCAAAA GTACTCTTTA ATTACAAGTT CTTTTTCATC TTCATCCAGC ACACTGATTT ATGTGCTACA CTGGGTTTGT ATTATATCTA TATTTTAATT CCACAACTAG ACACTAACTT TATTGAGAAC AGCGATATTC ATTTCTTCAA TTTCATTTGT GTTAACCCAA CTGTAGGTGA TAGTGCTCTG TACATCATAG TTACCCAGTG AATTACGTAC CTGACATATG ATTGCTTATT TCTGGTATTA AAACAAGATT TCATAAAATA AACATGACAA TTAAACTCGT TGTTTGAAGT ACTGAACTTT GTAGGTCAAT CTTGAAAAAG GAAGCTACAT GACTGTCTTA GCAATATTTA TAGGTATATT ATATGTAAGT GGGTGGGTTT CTTGGAAATT GAATTGGTAA TATGGCTTTA GAGTAGTAAC ACAAAAACTA TAGCTCTGTG CTCTCAGCTA ACCACTTTTA GTGTCACCTG GGCTGCTTAT TAAAAATTCA AATTTCTTAT CCCTACCCAG ATCAGAATCA AAATACCTGG GGGTTGTAGC TCCAGGTACC TACATTTCAA CAAGCACCCC AGATAATTGT GATGCACACT GAAGAACTAC TCTAGCTTGT AATCTCAGGT GGTAAGGCAT TTTATATATA CATATATATA TACACACACA CAAGCACAAA TGGTAATTCT ATGGGAAAAT GGAAATCAGC AAGTGAAGTG AAATGTCAAT TACCATCAGG AACAGGAAGC TAGTTCACCA TTACGCATCA GGGAACTGGA AGAATTCTTG AGATTTATTT TTTTGGGTGA TATATTCTTG GCAGTTTATC AGGCCTCATT TGCAATTATG GTCTGTCCCT TTTGAAGCCC TCAGATAGTT CGCTGGCATT TTAATTTTAA TAGCAATTTC TTGCATCTGT TGGAACAGCA TTCGTATGAC CGGCTTATAA GCAATGATCA GGGCTAGTAA GATGTCACAA AGCTGCGGTG ACTTTTCCTT ATCAGTTGGG GGTTTTTTTG CCATGATACC ATCGTGGAAA TGAAAGACAA CGTGAAAATC CTGCGTCCTT GTGATATTTC ATCAGTGGGA GTGAGCGGGG ATCTTAACGC AGTAAGAGGC AAGATAAACG GATGAGAGGC ATTAGTTCAG GCTTACAGGA GCAAGATGCT TTTAGTCAAA GTGTTCAAGA TAGCTCTCTT AAAGAGGAGC AGTGAAACCC AAAAATGAGT GTCTTTTGAG GGCAGCTGGA AGATGACCCG AGGGGAACTT TTATCCTGGG GTGGAGTCCT GCGAAGCAAG CCGCAGGTGA GGGCGTGGGC CAATTCTGCG CCTCACTGCT GTTAGGCGCA AGGACATCCC GAAACACACT CAACGACACC AGCAGCGAGA CTGACAGAAG CGGATCGCCA GCGGGAGCAA CAACTTGAGG CGAGCCGTTA GCCGCCTCGC CTTCCTCGCA GCGCCCCTCC CGCGCCCCGG GAAGCCCGTT CGGCTCCGCC TTTTCGACTG ACGTCCCGGG CGAGCGGGGG CGGGCGCGTG GGTGGGCCGA GCCGTCTGCA GCCAGCGATT CGGCTGGCTC TGCCACACCA CCGCGCGCCC CCGCTCCGCC CGCCCCTCCG GGCGCGTCTT TTCCGGGCTC GCGCTGAGTC CCGCCTCCGC CGGCTGTCCG GGTGCGCGCG CGCCGCTGCG GCTTTTTCTC TGGCCTCCGC CGCGCGCTCC TCCTCGTCCC AGCGCTAGCG GGCACGCGGT TCCTTTTTGC GAGCTTTCCG AGTGCCAGGC GCCGGCCGGC TGCGAAGACG CGGTGGGCCG CCCCTCCGGT GAGTATCCCT GGATGGGGAG GTTGCGGCCC CGGCACCCCT GCCCTGTCGG TACCAGGGCC CCATTTCCAG AGGGGGACTG AAGCTTGGCA ATCCGGTACT GTTGGTAAAG CCACCATGGA AGATGCCAAA AACATTAAGA AGGGCCCAGC GCCATTCTAC CCACTCGAAG ACGGGACCGC CGGCGAGCAG CTGCACAAAG CCATGAAGCG CTACGCCCTG GTGCCCGGCA CCATCGCCTT TACCGACGCA CATATCGAGG TGGACATTAC CTACGCCGAG TACTTCGAGA TGAGCGTTCG GCTGGCAGAA GCTATGAAGC GCTATGGGCT GAATACAAAC CATCGGATCG TGGTGTGCAG CGAGAATAGC TTGCAGTTCT TCATGCCCGT GTTGGGTGCC CTGTTCATCG GTGTGGCTGT GGCCCCAGCT AACGACATCT ACAACGAGCG CGAGCTGCTG AACAGCATGG GCATCAGCCA GCCCACCGTC GTATTCGTGA GCAAGAAAGG GCTGCAAAAG ATCCTCAACG TGCAAAAGAA GCTACCGATC ATACAAAAGA TCATCATCAT GGATAGCAAG ACCGACTACC AGGGCTTCCA AAGCATGTAC ACCTTCGTGA CTTCCCATTT GCCACCCGGC TTCAACGAGT ACGACTTCGT GCCCGAGAGC TTCGACCGGG ACAAAACCAT CGCCCTGATC ATGAACAGTA GTGGCAGTAC CGGATTGCCC AAGGGCGTAG CCCTACCGCA CCGCACCGCT TGTGTCCGAT TCAGTCATGC CCGCGACCCC ATCTTCGGCA ACCAGATCAT CCCCGACACC GCTATCCTCA GCGTGGTGCC ATTTCACCAC GGCTTCGGCA TGTTCACCAC GCTGGGCTAC TTGATCTGCG GCTTTCGGGT CGTGCTCATG TACCGCTTCG AGGAGGAGCT ATTCTTGCGC AGCTTGCAAG ACTATAAGAT TCAATCTGCC CTGCTGGTGC CCACACTATT TAGCTTCTTC GCTAAGAGCA CTCTCATCGA CAAGTACGAC CTAAGCAACT TGCACGAGAT CGCCAGCGGC GGGGCGCCGC TCAGCAAGGA GGTAGGTGAG GCCGTGGCCA AACGCTTCCA CCTACCAGGC ATCCGCCAGG GCTACGGCCT GACAGAAACA ACCAGCGCCA TTCTGATCAC CCCCGAAGGG GACGACAAGC CTGGCGCAGT AGGCAAGGTG GTGCCCTTCT TCGAGGCTAA GGTGGTGGAC TTGGACACCG GTAAGACACT GGGTGTGAAC CAGCGCGGCG AGCTGTGCGT CCGTGGCCCC ATGATCATGA GCGGCTACGT TAACAACCCC GAGGCTACAA ACGCTCTCAT CGACAAGGAC GGCTGGCTGC ACAGCGGCGA CATCGCCTAC TGGGACGAGG ACGAGCACTT CTTCATCGTG GACCGGCTGA AGAGCCTGAT CAAATACAAG GGCTACCAGG TAGCCCCAGC CGAACTGGAG AGCATCCTGC TGCAACACCC CAACATCTTC GACGCCGGGG TCGCCGGCCT GCCCGACGAC GATGCCGGCG AGCTGCCCGC CGCAGTCGTC GTGCTGGAAC ACGGTAAAAC CATGACCGAG AAGGAGATCG TGGACTATGT GGCCAGCCAG GTTACAACCG CCAAGAAGCT GCGCGGTGGT GTTGTGTTCG TGGACGAGGT GCCTAAAGGA CTGACCGGCA AGTTGGACGC CCGCAAGATC CGCGAGATTC TCATTAAGGC CAAGAAGGGC GGCAAGATCG CCGTGTAATA ATTCTAGAGT CGGGGCGGCC GGCCGCTTCG AGCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTAAAATCG ATAAGGATCC GTCGACCGAT GCCCTTGAGA GCCTTCAACC CAGTCAGCTC CTTCCGGTGG GCGCGGGGCA TGACTATCGT CGCCGCACTT ATGACTGTCT TCTTTATCAT GCAACTCGTA GGACAGGTGC CGGCAGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG CGGCCGCAAA TGCTAAACCA CTGCAGTGGT TACCAGTGCT TGATCAGTGA GGCACCGATC TCAGCGATCT GCCTATTTCG TTCGTCCATA GTGGCCTGAC TCCCCGTCGT GTAGATCACT ACGATTCGTG AGGGCTTACC ATCAGGCCCC AGCGCAGCAA TGATGCCGCG AGAGCCGCGT TCACCGGCCC CCGATTTGTC AGCAATGAAC CAGCCAGCAG GGAGGGCCGA GCGAAGAAGT GGTCCTGCTA CTTTGTCCGC CTCCATCCAG TCTATGAGCT GCTGTCGTGA TGCTAGAGTA AGAAGTTCGC CAGTGAGTAG TTTCCGAAGA GTTGTGGCCA TTGCTACTGG CATCGTGGTA TCACGCTCGT CGTTCGGTAT GGCTTCGTTC AACTCTGGTT CCCAGCGGTC AAGCCGGGTC ACATGATCAC CCATATTATG AAGAAATGCA GTCAGCTCCT TAGGGCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCGGT GTTGTCGCTC ATGGTAATGG CAGCACTACA CAATTCTCTT ACCGTCATGC CATCCGTAAG ATGCTTTTCC GTGACCGGCG AGTACTCAAC CAAGTCGTTT TGTGAGTAGT GTATACGGCG ACCAAGCTGC TCTTGCCCGG CGTCTATACG GGACAACACC GCGCCACATA GCAGTACTTT GAAAGTGCTC ATCATCGGGA ATCGTTCTTC GGGGCGGAAA GACTCAAGGA TCTTGCCGCT ATTGAGATCC AGTTCGATAT AGCCCACTCT TGCACCCAGT TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCGGGGT GTGCAAAAAC AGGCAAGCAA AATGCCGCAA AGAAGGGAAT GAGTGCGACA CGAAAATGTT GGATGCTCAT ACTCGTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TACTAGTACG TCTCTCAAGG ATAAGTAAGT AATATTAAGG TACGGGAGGT ATTGGACAGG CCGCAATAAA ATATCTTTAT TTTCATTACA TCTGTGTGTT GGTTTTTTGT GTGAATCGAT AGTACTAACA TACGCTCTCC ATCAAAACAA AACGAAACAA AACAAACTAG CAAAATAGGC TGTCCCCAGT GCAAGTGCAG GTGCCAGAAC ATTTCTCT
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