NM_002473.6;BDPLT6; DFNA17; EPSTS; FTNS; MATINS; MHA;NMHC-II-A; NMMHC-IIA; NMMHCA;
100μg liquid plasmid(Instantly):$149
| name | pCMV-MYH9(human)-3×Myc-Neo | another name | apCMV-MYH9(human)-3×Myc-Neo |
| Insert Size (bp) | 5880bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pCDNA3.1-3×Myc-C | 5′ sequencing primer | BGH |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC CACTAGTCCA GTGTGGTGGA ATTCGCCACC ATGGCACAGC AAGCTGCCGA TAAGTATCTC TATGTGGATA AAAACTTCAT CAACAATCCG CTGGCCCAGG CCGACTGGGC TGCCAAGAAG CTGGTATGGG TGCCTTCCGA CAAGAGTGGC TTTGAGCCAG CCAGCCTCAA GGAGGAGGTG GGCGAAGAGG CCATCGTGGA GCTGGTGGAG AATGGGAAGA AGGTGAAGGT GAACAAGGAT GACATCCAGA AGATGAACCC GCCCAAGTTC TCCAAGGTGG AGGACATGGC AGAGCTCACG TGCCTCAACG AAGCCTCGGT GCTGCACAAC CTCAAGGAGC GTTACTACTC AGGGCTCATC TACACCTATT CAGGCCTGTT CTGTGTGGTC ATCAATCCTT ACAAGAACCT GCCCATCTAC TCTGAAGAGA TTGTGGAAAT GTACAAGGGC AAGAAGAGGC ACGAGATGCC CCCTCACATC TATGCCATCA CAGACACCGC CTACAGGAGT ATGATGCAAG ACCGAGAAGA TCAATCCATC TTGTGCACTG GTGAATCTGG AGCTGGCAAG ACGGAGAACA CCAAGAAGGT CATCCAGTAT CTGGCGTACG TGGCGTCCTC GCACAAGAGC AAGAAGGACC AGGGCGAGCT GGAGCGGCAG CTGCTGCAGG CCAACCCCAT CCTGGAGGCC TTCGGGAACG CCAAGACCGT GAAGAATGAC AACTCCTCCC GCTTCGGCAA ATTCATTCGC ATCAACTTTG ATGTCAATGG CTACATTGTT GGAGCCAACA TTGAGACTTA TCTTTTGGAG AAATCTCGTG CTATCCGCCA AGCCAAGGAA GAACGGACCT TCCACATCTT CTATTATCTC CTGTCTGGGG CTGGAGAGCA CCTGAAGACC GATCTCCTGT TGGAGCCGTA CAACAAATAC CGCTTCCTGT CCAATGGACA CGTCACCATC CCCGGGCAGC AGGACAAGGA CATGTTCCAG GAGACCATGG AGGCCATGAG GATTATGGGC ATCCCAGAAG AGGAGCAAAT GGGCCTGCTG CGGGTCATCT CAGGGGTTCT TCAGCTCGGC AACATCGTCT TCAAGAAGGA GCGGAACACT GACCAGGCGT CCATGCCCGA CAACACAGCT GCCCAAAAGG TGTCCCATCT CTTGGGTATC AATGTGACCG ATTTCACCAG AGGAATCCTC ACCCCGCGCA TCAAGGTGGG ACGGGATTAC GTCCAGAAGG CGCAGACTAA AGAGCAGGCT GACTTTGCCA TCGAGGCCTT GGCCAAGGCG ACCTATGAGC GGATGTTCCG CTGGCTGGTG CTGCGCATCA ACAAGGCTCT GGACAAGACC AAGAGGCAGG GCGCCTCCTT CATCGGGATC CTGGACATTG CCGGCTTCGA GATCTTTGAT CTGAACTCGT TTGAGCAGCT GTGCATCAAT TACACCAATG AGAAGCTGCA GCAGCTCTTC AACCACACCA TGTTCATCCT GGAGCAGGAG GAGTACCAGC GCGAGGGCAT CGAGTGGAAC TTCATCGACT TTGGCCTCGA CCTGCAGCCC TGCATCGACC TCATTGAGAA GCCAGCAGGC CCCCCGGGCA TTCTGGCCCT GCTGGACGAG GAGTGCTGGT TCCCCAAAGC CACCGACAAG AGCTTCGTGG AGAAGGTGAT GCAGGAGCAG GGCACCCACC CCAAGTTCCA GAAGCCCAAG CAGCTGAAGG ACAAAGCTGA TTTCTGCATT ATCCACTATG CCGGCAAGGT GGATTACAAA GCTGACGAGT GGCTGATGAA GAACATGGAT CCCCTGAATG ACAACATCGC CACACTGCTC CACCAGTCCT CTGACAAGTT TGTCTCGGAG CTGTGGAAGG ATGTGGACCG CATCATCGGC CTGGACCAGG TGGCCGGCAT GTCGGAGACC GCACTGCCCG GGGCCTTCAA GACGCGGAAG GGCATGTTCC GCACTGTGGG GCAGCTTTAC AAGGAGCAGC TGGCCAAGCT GATGGCTACG CTGAGGAACA CGAACCCCAA CTTTGTCCGC TGCATCATCC CCAACCACGA GAAGAAGGCC GGCAAGCTGG ACCCGCATCT CGTGCTGGAC CAGCTGCGCT GCAACGGTGT TCTCGAGGGC ATCCGTATCT GCCGCCAGGG CTTCCCCAAC AGGGTGGTCT TCCAGGAGTT TCGGCAGAGA TATGAGATCC TGACTCCAAA CTCCATTCCC AAGGGTTTCA TGGACGGGAA GCAGGCGTGC GTGCTCATGA TAAAAGCCCT GGAGCTCGAC AGCAATCTGT ACCGCATTGG CCAGAGCAAA GTCTTCTTCC GTGCCGGTGT GCTGGCCCAC CTGGAGGAGG AGCGAGACCT GAAGATCACC GACGTCATCA TAGGGTTCCA GGCCTGCTGC AGGGGCTACC TGGCCAGGAA AGCATTTGCC AAGCGGCAGC AGCAGCTTAC CGCCATGAAG GTCCTCCAGC GGAACTGCGC TGCCTACCTG AAGCTGCGGA ACTGGCAGTG GTGGCGGCTC TTCACCAAGG TCAAGCCGCT GCTGCAGGTG AGCCGGCAGG AGGAGGAGAT GATGGCCAAG GAGGAGGAGC TGGTGAAGGT CAGAGAGAAG CAGCTGGCTG CGGAGAACAG GCTCACGGAG ATGGAGACGC TGCAGTCTCA GCTCATGGCA GAGAAATTGC AGCTGCAGGA GCAGCTCCAG GCAGAAACCG AGCTGTGTGC CGAGGCTGAG GAGCTCCGGG CCCGCCTGAC CGCCAAGAAG CAGGAATTAG AAGAGATCTG CCATGACCTA GAGGCCAGGG TGGAGGAGGA GGAGGAGCGC TGCCAGCACC TGCAGGCGGA GAAGAAGAAG ATGCAGCAGA ACATCCAGGA GCTTGAGGAG CAGCTGGAGG AGGAGGAGAG CGCCCGGCAG AAGCTGCAGC TGGAGAAGGT GACCACCGAG GCGAAGCTGA AAAAGCTGGA GGAGGAGCAG ATCATCCTGG AGGACCAGAA CTGCAAGCTG GCCAAGGAAA AGAAACTGCT GGAAGACAGA ATAGCTGAGT TCACCACCAA CCTCACAGAA GAGGAGGAGA AATCTAAGAG CCTCGCCAAG CTCAAGAACA AGCATGAGGC AATGATCACT GACTTGGAAG AGCGCCTCCG CAGGGAGGAG AAGCAGCGAC AGGAGCTGGA GAAGACCCGC CGGAAGCTGG AGGGAGACTC CACAGACCTC AGCGACCAGA TCGCCGAGCT CCAGGCCCAG ATCGCGGAGC TCAAGATGCA GCTGGCCAAG AAAGAGGAGG AGCTCCAGGC CGCCCTGGCC AGAGTGGAAG AGGAAGCTGC CCAGAAGAAC ATGGCCCTCA AGAAGATCCG GGAGCTGGAA TCTCAGATCT CTGAACTCCA GGAAGACCTG GAGTCTGAGC GTGCTTCCAG GAATAAAGCT GAGAAGCAGA AACGGGACCT TGGGGAAGAG CTAGAGGCTC TGAAAACAGA GTTGGAGGAC ACGCTGGATT CCACAGCTGC CCAGCAGGAG CTCAGGTCAA AACGTGAGCA GGAGGTGAAC ATCCTGAAGA AGACCCTGGA GGAGGAGGCC AAGACCCACG AGGCCCAGAT CCAGGAGATG AGGCAGAAGC ACTCACAGGC CGTGGAGGAG CTGGCGGAGC AGCTGGAGCA GACGAAGCGG GTGAAAGCAA ACCTCGAGAA GGCAAAGCAG ACTCTGGAGA ACGAGCGGGG GGAGCTGGCC AACGAGGTGA AGGTGCTGCT GCAGGGCAAA GGGGACTCGG AGCACAAGCG CAAGAAAGTG GAGGCGCAGC TGCAGGAGCT GCAGGTCAAG TTCAACGAGG GAGAGCGCGT GCGCACAGAG CTGGCCGACA AGGTCACCAA GCTGCAGGTG GAGCTGGACA ACGTGACCGG GCTTCTCAGC CAGTCCGACA GCAAGTCCAG CAAGCTCACC AAGGACTTCT CCGCGCTGGA GTCCCAGCTG CAGGACACTC AGGAGCTGCT GCAGGAGGAG AACCGGCAGA AGCTGAGCCT GAGCACCAAG CTCAAGCAGG TGGAGGACGA GAAGAATTCC TTCCGGGAGC AGCTGGAGGA GGAGGAGGAG GCCAAGCACA ACCTGGAGAA GCAGATCGCC ACCCTCCATG CCCAGGTGGC CGACATGAAA AAGAAGATGG AGGACAGTGT GGGGTGCCTG GAAACTGCTG AGGAGGTGAA GAGGAAGCTC CAGAAGGACC TGGAGGGCCT GAGCCAGCGG CACGAGGAGA AGGTGGCCGC CTACGACAAG CTGGAGAAGA CCAAGACGCG GCTGCAGCAG GAGCTGGACG ACCTGCTGGT GGACCTGGAC CACCAGCGCC AGAGCGCGTG CAACCTGGAG AAGAAGCAGA AGAAGTTTGA CCAGCTCCTG GCGGAGGAGA AGACCATCTC TGCCAAGTAT GCAGAGGAGC GCGACCGGGC TGAGGCGGAG GCCCGAGAGA AGGAGACCAA GGCTCTGTCG CTGGCCCGGG CCCTGGAGGA AGCCATGGAG CAGAAGGCGG AGCTGGAGCG GCTCAACAAG CAGTTCCGCA CGGAGATGGA GGACCTTATG AGCTCCAAGG ATGATGTGGG CAAGAGTGTC CACGAGCTGG AGAAGTCCAA GCGGGCCCTA GAGCAGCAGG TGGAGGAGAT GAAGACGCAG CTGGAAGAGC TGGAGGACGA GCTGCAGGCC ACCGAAGATG CCAAGCTGCG GTTGGAGGTC AACCTGCAGG CCATGAAGGC CCAGTTCGAG CGGGACCTGC AGGGCCGGGA CGAGCAGAGC GAGGAGAAGA AGAAGCAGCT GGTCAGACAG GTGCGGGAGA TGGAGGCAGA GCTGGAGGAC GAGAGGAAGC AGCGCTCGAT GGCAGTGGCC GCCCGGAAGA AGCTGGAGAT GGACCTGAAG GACCTGGAGG CGCACATCGA CTCGGCCAAC AAGAACCGGG ACGAAGCCAT CAAACAGCTG CGGAAGCTGC AGGCCCAGAT GAAGGACTGC ATGCGCGAGC TGGATGACAC CCGCGCCTCT CGTGAGGAGA TCCTGGCCCA GGCCAAAGAG AACGAGAAGA AGCTGAAGAG CATGGAGGCC GAGATGATCC AGTTGCAGGA GGAACTGGCA GCCGCGGAGC GTGCCAAGCG CCAGGCCCAG CAGGAGCGGG ATGAGCTGGC TGACGAGATC GCCAACAGCA GCGGCAAAGG AGCCCTGGCG TTAGAGGAGA AGCGGCGTCT GGAGGCCCGC ATCGCCCAGC TGGAGGAGGA GCTGGAGGAG GAGCAGGGCA ACACGGAGCT GATCAACGAC CGGCTGAAGA AGGCCAACCT GCAGATCGAC CAGATCAACA CCGACCTGAA CCTGGAGCGC AGCCACGCCC AGAAGAACGA GAATGCTCGG CAGCAGCTGG AACGCCAGAA CAAGGAGCTT AAGGTCAAGC TGCAGGAGAT GGAGGGCACT GTCAAGTCCA AGTACAAGGC CTCCATCACC GCCCTCGAGG CCAAGATTGC ACAGCTGGAG GAGCAGCTGG ACAACGAGAC CAAGGAGCGC CAGGCAGCCT GCAAACAGGT GCGTCGGACC GAGAAGAAGC TGAAGGATGT GCTGCTGCAG GTGGATGACG AGCGGAGGAA CGCCGAGCAG TACAAGGACC AGGCCGACAA GGCATCTACC CGCCTGAAGC AGCTCAAGCG GCAGCTGGAG GAGGCCGAAG AGGAGGCCCA GCGGGCCAAC GCCTCCCGCC GGAAACTGCA GCGCGAGCTG GAGGACGCCA CTGAGACGGC CGATGCCATG AACCGCGAAG TCAGCTCCCT AAAGAACAAG CTCAGGCGCG GGGACCTGCC GTTTGTCGTG CCCCGCCGAA TGGCCCGGAA AGGCGCCGGG GATGGCTCCG ACGAAGAGGT AGATGGCAAA GCGGATGGGG CTGAGGCCAA ACCTGCCGAA CTCGAGGAAC AAAAACTAAT ATCAGAGGAA GATTTGGAAC AGAAACTAAT CAGTGAAGAA GATTTAGAGC AAAAGTTAAT TTCCGAGGAG GACTTATGAG GGCCCGTTTA AACCCGCTGA TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC CCCCGTGCCT TCCTTGACCC TGGAAGGTGC CACTCCCACT GTCCTTTCCT AATAAAATGA GGAAATTGCA TCGCATTGTC TGAGTAGGTG TCATTCTATT CTGGGGGGTG GGGTGGGGCA GGACAGCAAG GGGGAGGATT GGGAAGACAA TAGCAGGCAT GCTGGGGATG CGGTGGGCTC TATGGCTTCT GAGGCGGAAA GAACCAGCTG GGGCTCTAGG GGGTATCCCC ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTA ATTCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CTGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CGACGGATCG GGAGATCTCC CGATCCCCTA TGGT
