NM_017672.6;ALSPDC/CHAK/CHAK1/LTrpC-7/LTRPC7/TRP-PLIK
100μg liquid plasmid(Instantly):$149
| name | pCMV-TRPM7(human-Toxic-opt)-3×FLAG-Neo | another name | apCMV-TRPM7(human-Toxic-opt)-3×FLAG-Neo |
| Insert Size (bp) | 5598bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | BL21(DE3) | Growth Temperature | 30°C |
| Vector backbone | pCMV-T7-MCS-3×FLAG-WPRE-Neo | 5′ sequencing primer | CMV-F |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCATGTC CCAGAAATCC TGGATAGAAA GCACTTTGAC CAAGAGGGAA TGTGTATATA TTATACCAAG TTCCAAGGAC CCTCACAGAT GCCTTCCAGG ATGTCAAATT TGTCAGCAAC TCGTCAGGTG TTTTTGTGGT CGCTTGGTCA AGCAACATGC TTGTTTTACT GCAAGTCTTG CCATGAAATA CTCAGATGTG AAATTGGGTG ACCATTTTAA TCAGGCAATA GAAGAATGGT CTGTGGAAAA GCATACAGAA CAGAGCCCAA CGGATGCTTA TGGAGTCATA AATTTTCAAG GGGGTTCTCA TTCCTACAGA GCTAAGTATG TGAGGCTATC ATATGACACC AAACCTGAAG TCATTCTGCA ACTTCTGCTT AAAGAATGGC AAATGGAGTT ACCCAAACTT GTTATCTCTG TACATGGGGG CATGCAGAAA TTTGAGCTTC ACCCACGAAT CAAGCAGTTG CTTGGAAAAG GTCTTATTAA AGCTGCAGTT ACAACTGGAG CCTGGATTTT AACTGGAGGA GTAAACACAG GTGTGGCAAA ACATGTTGGA GATGCCCTCA AAGAACATGC TTCCAGATCA TCTCGAAAGA TTTGCACTAT CGGAATAGCT CCATGGGGAG TGATTGAAAA CAGAAATGAT CTTGTTGGGA GAGATGTGGT TGCTCCTTAT CAAACCTTAT TGAACCCCCT GAGCAAATTG AATGTTTTGA ATAATCTGCA TTCCCATTTC ATATTGGTGG ATGATGGCAC TGTTGGAAAG TATGGGGCGG AAGTCAGACT GAGAAGAGAA CTTGAAAAAA CTATTAATCA GCAAAGAATT CATGCTAGGA TTGGCCAGGG TGTCCCTGTG GTGGCACTTA TATTTGAGGG TGGGCCAAAT GTTATCCTCA CAGTTCTTGA ATACCTTCAG GAAAGCCCCC CTGTTCCAGT AGTTGTGTGT GAAGGAACAG GCAGAGCTGC AGATCTGCTA GCGTATATTC ATAAACAAAC AGAAGAAGGA GGGAATCTTC CTGATGCAGC AGAGCCCGAT ATTATTTCCA CTATCAAAAA AACATTTAAC TTTGGCCAGA ATGAAGCACT TCATTTATTT CAAACACTGA TGGAGTGCAT GAAAAGAAAG GAGCTTATCA CTGTTTTCCA TATTGGGTCA GATGAACATC AAGATATAGA TGTAGCAATA CTTACTGCAC TGCTAAAAGG TACTAATGCA TCTGCATTTG ACCAGCTTAT CCTTACATTG GCATGGGATA GAGTTGACAT TGCCAAAAAT CATGTATTTG TTTATGGACA GCAGTGGCTG GTTGGATCCT TGGAACAAGC TATGCTTGAT GCTCTTGTAA TGGATAGAGT TGCATTTGTA AAACTTCTTA TTGAAAATGG AGTAAGCATG CATAAATTCC TTACCATTCC GAGACTGGAA GAACTTTACA ACACTAAACA AGGTCCAACT AATCCAATGC TGTTTCATCT TGTTCGAGAC GTCAAACAGG GAAATCTTCC TCCAGGATAT AAGATCACTC TGATTGATAT AGGACTTGTT ATTGAATATC TCATGGGAGG AACCTACAGA TGCACCTATA CTAGGAAACG TTTTCGATTA ATATATAATA GTCTTGGTGG AAATAATCGG AGGTCTGGCC GAAATACCTC CAGCAGCACT CCTCAGTTGC GAAAGAGTCA TGAATCTTTT GGCAATAGGG CAGATAAAAA GGAAAAAATG AGGCATAACC ATTTCATTAA GACAGCACAG CCCTACCGAC CAAAGATTGA TACAGTTATG GAAGAAGGAA AGAAGAAAAG AACCAAAGAT GAAATTGTAG ACATTGATGA TCCAGAAACC AAGCGCTTTC CTTATCCACT TAATGAACTT TTAATTTGGG CTTGCCTTAT GAAGAGGCAG GTCATGGCCC GTTTTTTATG GCAACATGGT GAAGAATCAA TGGCTAAAGC ATTAGTTGCC TGTAAGATCT ATCGTTCAAT GGCATATGAA GCAAAGCAGA GTGACCTGGT AGATGATACT TCAGAAGAAC TAAAACAGTA TTCCAATGAT TTTGGTCAGT TGGCCGTTGA ATTATTAGAA CAGTCCTTCA GACAAGATGA AACCATGGCT ATGAAATTGC TCACTTATGA ACTGAAGAAC TGGAGTAATT CAACCTGCCT TAAGTTAGCA GTTTCTTCAA GACTTAGACC TTTTGTAGCT CACACCTGTA CACAAATGCT TCTGAGCGAT ATGTGGATGG GCAGACTGAA TATGCGAAAG AACTCATGGT ATAAGGTGAT TCTCAGTATA CTGGTCCCAC CAGCGATCCT GTTGTTGGAA TACAAGACAA AGGCTGAAAT GTCTCACATT CCACAGTCAC AAGATGCGCA CCAGATGACC ATGGACGATT CTGAGAATAA CTTCCAGAAT ATAACAGAAG AGATCCCAAT GGAGGTTTTC AAAGAGGTGC GCATCCTCGA TAGCAACGAA GGGAAAAACG AGATGGAAAT CCAAATGAAA TCAAAAAAGC TCCCGATCAC GCGGAAGTTT TACGCCTTTT ACCATGCACC GATCGTAAAG TTCTGGTTCA ACACCCTGGC ATATCTGGGC TTCCTGATGT TGTATACATT TGTGGTGCTG GTCCAGATGG AACAGTTGCC TTCCGTGCAG GAATGGATCG TCATAGCTTA CATCTTCACA TACGCTATCG AAAAGGTCAG AGAGATTTTT ATGAGTGAGG CAGGTAAGGT CAACCAAAAG ATCAAGGTGT GGTTCTCTGA CTACTTTAAC ATCTCCGATA CTATCGCCAT CATCTCTTTC TTTATAGGGT TTGGCCTGAG GTTTGGCGCA AAGTGGAACT TCGCCAACGC TTACGATAAT CATGTGTTTG TCGCTGGTCG ACTCATCTAT TGCCTTAATA TCATCTTCTG GTACGTCAGA CTGTTGGACT TCCTCGCGGT CAATCAACAG GCTGGACCCT ACGTGATGAT GATTGGCAAG ATGGTAGCCA ATATGTTCTA TATCGTCGTG ATCATGGCAC TCGTACTGCT GTCCTTCGGA GTACCACGCA AGGCAATTCT TTACCCTCAC GAAGCCCCTT CTTGGACTTT GGCCAAGGAC ATTGTGTTCC ACCCATACTG GATGATCTTT GGTGAAGTCT ATGCTTACGA GATTGACGTC TGTGCCAATG ATTCCGTGAT CCCACAGATC TGTGGACCCG GTACCTGGCT CACGCCTTTC TTGCAAGCCG TCTATCTGTT CGTTCAGTAC ATTATAATGG TTAATCTTCT TATTGCATTT TTCAACAATG TGTATTTACA AGTGAAGGCA ATTTCCAATA TTGTATGGAA GTACCAGCGT TATCATTTTA TTATGGCTTA TCATGAGAAA CCAGTTCTGC CTCCTCCACT TATCATTCTT AGCCATATAG TTTCTCTGTT TTGCTGCATA TGTAAGAGAA GAAAGAAAGA TAAGACTTCC GATGGACCAA AACTTTTCTT AACAGAAGAA GATCAAAAGA AACTTCATGA TTTTGAAGAG CAGTGTGTTG AAATGTATTT CAATGAAAAA GATGACAAAT TTCATTCTGG GAGTGAAGAG AGAATTCGTG TCACTTTTGA AAGAGTGGAA CAGATGTGCA TTCAGATTAA AGAAGTTGGA GATCGTGTCA ACTACATAAA AAGATCATTA CAATCATTAG ATTCTCAAAT TGGCCATTTG CAAGATCTTT CAGCCCTGAC GGTAGATACA TTAAAAACAC TCACTGCCCA GAAAGCGTCG GAAGCTAGCA AAGTTCATAA TGAAATCACA CGAGAACTGA GCATTTCCAA ACACTTGGCT CAAAACCTTA TTGATGATGG TCCTGTAAGA CCTTCTGTAT GGAAAAAGCA TGGTGTTGTA AATACACTTA GCTCCTCTCT TCCTCAAGGT GATCTTGAAA GTAATAATCC TTTTCATTGT AATATTTTAA TGAAAGATGA CAAAGATCCC CAGTGTAATA TATTTGGTCA AGACTTACCT GCAGTACCCC AGAGAAAAGA ATTTAATTTT CCAGAGGCTG GTTCCTCTTC TGGTGCCTTA TTCCCAAGTG CTGTTTCCCC TCCAGAACTG CGACAGAGAC TACATGGGGT AGAACTCTTA AAAATATTTA ATAAAAATCA AAAATTAGGC AGTTCATCTA CTAGCATACC ACATCTGTCA TCCCCACCAA CCAAATTTTT TGTTAGTACA CCATCTCAGC CAAGTTGCAA AAGCCACTTG GAAACTGGAA CCAAAGATCA AGAAACTGTT TGCTCTAAAG CTACAGAAGG AGATAATACA GAATTTGGAG CATTTGTAGG ACACAGAGAT AGCATGGATT TACAGAGGTT TAAAGAAACA TCAAACAAGA TAAAAATACT ATCCAATAAC AATACTTCTG AAAACACTTT GAAACGAGTG AGTTCTCTTG CTGGATTTAC TGACTGTCAC AGAACTTCCA TTCCTGTTCA TTCAAAACAA GCAGAAAAAA TCAGTAGAAG GCCATCTACC GAAGACACTC ATGAAGTAGA TTCCAAAGCA GCTTTAATAC CGGATTGGTT ACAAGATAGA CCATCAAACA GAGAAATGCC ATCTGAAGAA GGAACATTAA ATGGTCTCAC TTCTCCATTT AAGCCAGCTA TGGATACAAA TTACTATTAT TCAGCTGTGG AAAGAAATAA CTTGATGAGG TTATCACAGA GCATTCCATT TACACCTGTG CCTCCAAGAG GGGAGCCTGT CACAGTGTAT CGTTTGGAAG AGAGTTCACC CAACATACTA AATAACAGCA TGTCTTCTTG GTCACAACTA GGCCTCTGTG CCAAAATAGA GTTTTTAAGC AAAGAGGAGA TGGGAGGAGG TTTACGAAGA GCTGTCAAAG TACAGTGTAC CTGGTCAGAA CATGATATCC TCAAATCAGG GCATCTTTAT ATTATCAAAT CTTTTCTTCC AGAGGTGGTT AATACATGGT CAAGTATTTA CAAAGAAGAT ACAGTTCTGC ATCTCTGTCT GAGAGAAATT CAACAACAGA GAGCAGCACA AAAGCTTACG TTTGCCTTTA ATCAAATGAA ACCCAAATCC ATACCATATT CTCCAAGGTT CCTTGAAGTT TTCCTGCTGT ATTGCCATTC AGCAGGACAG TGGTTTGCTG TGGAAGAATG TATGACTGGA GAATTTAGAA AATACAACAA TAATAATGGA GATGAGATTA TTCCAACTAA TACTCTGGAA GAGATCATGC TAGCCTTTAG CCACTGGACT TACGAATATA CAAGAGGGGA GTTACTGGTA CTTGATTTGC AAGGTGTTGG TGAAAATTTG ACTGACCCAT CTGTGATAAA AGCAGAAGAA AAGAGATCCT GTGATATGGT TTTTGGCCCA GCAAATCTAG GAGAAGATGC AATTAAAAAC TTCAGAGCAA AACATCACTG TAATTCTTGC TGTAGAAAGC TTAAACTTCC AGATCTGAAG AGGAATGATT ATACGCCTGA TAAAATTATA TTTCCTCAGG ATGAGCCTTC AGATTTGAAT CTTCAGCCTG GAAATTCCAC CAAAGAATCA GAATCAACTA ATTCTGTTCG TCTGATGTTA GGATCCGACT ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA CTACAAGGAT GACGATGACA AGTAGTGAGG GCCCGATATC TCGACAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT TGTCGGGGAA GCTGACGTCC TTTCCATGGC TGCTCGCCTG TGTTGCCACC TGGATTCTGC GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC CCGCCTGGAA ACGGGGGAGG CTAACTGAAA CACGGAAGGA GACAATACCG GAAGGAACCC GCGCTATGAC GGCAATAAAA AGACAGAATA AAACGCACGG GTGTTGGGTC GTTTGTTCAT AAACGCGGGG TTCGGTCCCA GGGCTGGCAC TCTGTCGATA CCCCACCGAG ACCCCATTGG GGCCAATACG CCCGCGTTTC TTCCTTTTCC CCACCCCACC CCCCAAGTTC GGGTGAAGGC CCAGGGCTCG CAGCCAACGT CGGGGCGGCA GGCCCTGCCA TAGCAGATCT GCGCAGCTGG GGCTCTAGGG GGTATCCCCA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGCATC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG GGGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTAA TTCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC TGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCAATGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCGACGG ATCGGGAGAT CTCCCGATCC CCTATGGTCG ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATCTGCT CCCTGCTTGT GTGTTGGAGG TCGCTGAGTA GTGCGCGAGC AAAATTTAAG CTACAACAAG GCAAGGCTTG ACCGACAATT GCATGAAGAA TCTGCTTAGG
