pCMV-CNTNAP4(human)-Myc-6×His
$99.00
In stock
SKU
PL21753
NM_033401.5;CNTNAP4/CASPR4
100μg liquid plasmid(Instantly):$120
| name | pCMV-CNTNAP4(human)-Myc-6×His | another name | apCMV-CNTNAP4(human)-Myc-6×His |
| Insert Size (bp) | 3924bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pCDNA4-Myc-HisB | 5′ sequencing primer | CMV-F |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAC TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGT TAAGCTTGGT ACCGAGCTCG GATCCGCCAC CATGGGATCT GTCACGGGAG CTGTCCTCAA GACGCTACTT CTGTTATCTA CTCAAAATTG GAACAGAGTC GAAGCTGGGA ATTCCTATGA CTGTGATGAT CCTCTTGTGT CTGCCTTGCC TCAGGCATCC TTCAGCAGTT CTTCCGAGCT CTCCAGCAGT CATGGTCCTG GATTTGCAAG GCTGAATAGA AGAGATGGAG CTGGTGGCTG GTCTCCACTT GTGTCTAACA AATACCAGTG GTTGCAGATT GACCTTGGAG AGAGAATGGA GGTCACCGCT GTGGCCACTC AAGGGGGATA TGGTAGCTCC AACTGGGTGA CCAGCTACCT CCTGATGTTC AGTGATAGTG GCTGGAACTG GAAACAATAT CGCCAAGAGG ACAGCATCTG GGGTTTTTCA GGAAATGCAA ATGCAGACAG TGTTGTGTAC TATAGACTCC AGCCTTCTAT CAAAGCCAGA TTTCTGCGCT TCATCCCTTT GGAATGGAAC CCCAAGGGCA GAATTGGAAT GCGAATCGAA GTGTTCGGAT GTGCATACAG ATCAGAAGTG GTTGATCTTG ATGGAAAAAG TTCCCTTCTC TACAGATTTG ATCAAAAATC CCTGAGCCCA ATAAAAGACA TTATTTCTTT GAAATTCAAA ACCATGCAGA GTGATGGGAT TCTACTCCAC AGGGAAGGGC CAAATGGAGA TCACATCACA CTGCAATTAA GAAGAGCAAG ACTCTTTTTA CTTATTAATT CAGGTGAAGC TAAACTGCCT TCCACTTCCA CCCTGGTCAA TCTCACCCTG GGCAGCCTGC TAGATGATCA GCATTGGCAT TCAGTGCTCA TCCAGCGTTT GGGCAAACAA GTCAACTTCA CAGTGGACGA ACACAGGCAT CATTTCCATG CACGGGGAGA ATTCAATCTC ATGAATCTTG ATTATGAGAT CAGCTTTGGA GGGATTCCAG CACCTGGAAA ATCAGTGTCA TTCCCACATA GAAATTTTCA TGGATGTTTA GAAAATCTCT ATTATAATGG AGTGGATATC ATTGATTTGG CCAAGCAGCA AAAACCACAG ATCATTGCTA TGGGAAATGT GTCATTTTCT TGTTCACAAC CACAATCTAT GCCCGTGACT TTTCTGAGCT CCAGGAGTTA TTTAGCACTG CCAGACTTCT CTGGAGAGGA GGAGGTTTCT GCCACTTTTC AATTTCGAAC TTGGAATAAG GCAGGGCTTC TGCTGTTCAG TGAACTTCAG CTGATTTCAG GGGGTATCCT CCTCTTTCTG AGTGATGGAA AACTTAAGTC GAATCTCTAC CAGCCAGGAA AATTACCCAG TGACATCACA GCAGGTGTCG AATTAAATGA TGGGCAGTGG CATTCTGTCT CTTTATCTGC TAAAAAGAAT CACTTGAGTG TGGCGGTGGA CGGCCAGATG GCTTCTGCTG CTCCTCTGCT GGGGCCTGAG CAGATTTATT CGGGTGGCAC CTATTATTTT GGAGGTTGTC CTGACAAAAG CTTTGGATCC AAATGTAAAA GTCCACTTGG TGGATTTCAG GGATGTATGA GGCTCATTTC TATCAGCGGC AAAGTGGTAG ATCTGATTTC AGTTCAGCAG GGGTCCCTTG GGAACTTCAG TGACCTTCAG ATAGACTCAT GTGGCATCTC AGACAGGTGT TTGCCCAACT ATTGTGAACA CGGTGGGGAG TGTTCCCAGT CCTGGAGCAC CTTTCATTGT AACTGTACCA ACACTGGTTA CAGAGGAGCT ACTTGCCATA ACTCTATCTA TGAGCAGTCA TGTGAAGCCT ATAAGCACAG AGGAAATACT TCAGGGTTTT ACTATATAGA TTCAGATGGA AGTGGTCCCC TGGAACCATT TCTTCTATAT TGCAATATGA CCGAAACTGC ATGGACCATC ATACAGCACA ACGGCTCTGA CTTAACAAGA GTCAGAAATA CTAATCCAGA GAACCCATAT GCTGGGTTTT TCGAGTATGT GGCCAGCATG GAGCAACTTC AGGCCACTAT TAACCGTGCA GAGCACTGTG AACAGGAGTT TACTTATTAC TGCAAGAAGT CACGGCTGGT CAATAAGCAA GATGGAACCC CTCTGAGTTG GTGGGTAGGA AGAACCAATG AAACGCAAAC CTACTGGGGA GGTTCTTCGC CTGATCTTCA AAAATGTACT TGTGGATTAG AGGGAAACTG CATTGATTCT CAGTATTACT GCAATTGTGA TGCTGACCGG AATGAATGGA CCAATGACAC TGGATTGCTT GCTTATAAAG AACATCTTCC AGTAACTAAG ATCGTGATTA CAGACACAGG CCGACTGCAT TCAGAAGCAG CTTATAAACT GGGGCCTCTG CTCTGCCAGG GAGACAGATC ATTTTGGAAT TCAGCTTCCT TTGATACCGA GGCTTCATAT CTTCATTTTC CTACCTTCCA CGGAGAACTT AGCGCGGATG TATCTTTCTT TTTTAAGACA ACAGCTTCAT CTGGGGTATT TTTAGAGAAC TTGGGGATTG CTGATTTTAT ACGGATAGAG CTTCGCTCTC CGACAGTAGT GACTTTTTCA TTTGATGTGG GGAATGGGCC TTTTGAAATC TCAGTGCAGT CACCCACCCA CTTCAACGAC AACCAGTGGC ACCATGTGAG GGTTGAAAGG AACATGAAGG AGGCCTCCCT TCAAGTGGAT CAGCTGACAC CAAAGACACA GCCCGCCCCC GCTGATGGGC ATGTCCTGTT ACAGCTCAAC AGTCAGCTCT TCGTGGGTGG AACGGCCACC AGACAGAGAG GCTTTCTGGG CTGCATTCGG TCTCTGCAGT TGAATGGGAT GACCCTGGAT TTGGAAGAAA GAGCCCAGGT GACTCCAGAA GTGCAGCCAG GTTGTAGGGG ACATTGCAGC AGCTATGGGA AGTTATGCCG CAATGGAGGG AAATGCAGAG AAAGACCCAT TGGGTTCTTT TGTGACTGCA CTTTCTCTGC ATACACAGGG CCATTCTGCT CAAATGAGAT TTCTGCATAT TTTGGATCTG GCTCATCCGT GATATACAAT TTTCAAGAAA ATTATCTTTT AAGTAAAAAC TCCAGCTCCC ACGCTGCTTC ATTTCATGGT GATATGAAGC TGAGCAGAGA AATGATCAAA TTTAGTTTCC GAACAACACG AACACCAAGC TTGCTGCTTT TTGTGAGCTC CTTTTACAAA GAATACCTTT CTGTGATCAT TGCCAAAAAT GGAAGTTTGC AGATCAGGTA CAAGTTAAAT AAATATCAAG AGCCTGATGT TGTTAACTTT GATTTTAAAA ACATGGCTGA TGGACAACTT CACCACATAA TGATTAACAG AGAAGAAGGA GTGGTCTTTA TAGAGATTGA CGATAATAGA AGGAGACAAG TTCACCTGTC ATCAGGCACA GAATTCAGTG CAGTCAAATC TCTGGTATTG GGCAGGATTT TAGAACACAG TGATGTGGAC CAGGATACTG CACTGGCAGG TGCGCAGGGC TTCACAGGCT GCCTCTCTGC AGTGCAGCTC AGCCACGTGG CCCCTCTGAA GGCAGCTCTG CACCCCAGCC ACCCAGACCC TGTCACTGTT ACAGGACACG TGACTGAGTC CAGCTGTATG GCCCAGCCTG GCACTGATGC CACATCAAGG GAAAGGACAC ACTCGTTTGC AGATCATTCT GGAACAATAG ATGACAGAGA GCCCCTTGCT AATGCAATCA AAAGTGACTC TGCAGTAATT GGAGGTCTGA TAGCTGTTGT GATTTTTATC TTGCTTTGCA TCACTGCCAT AGCTGTTCGC ATTTATCAGC AGAAAAGGTT ATATAAAAGA AGTGAGGCAA AAAGGTCAGA GAATGTAGAC AGTGCTGAGG CTGTTCTGAA AAGTGAGCTT AATATACAAA ATGCAGTCAA TGAAAATCAG AAAGAGTACT TCTTCCTCGA GGAACAAAAA CTCATCTCAG AAGAGGATCT GAATATGAAT ACCGGTCATC ATCACCATCA CCATTGAGTT TAAACCCGCT GATCAGCCTC GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG TCTGAGTAGG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA TTGGGAAGAC AATAGCAGGC ATGCTGGGGA TGCGGTGGGC TCTATGGCTT CTGAGGCGGA AAGAACCAGC TGGGGCTCTA GGGGGTATCC CCACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGC ATCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGGGGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TAATTCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCTGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAGCAC GTGTTGACAA TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC AAGGTGAGGA ACTAAACCAT GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC GACGTCGCCG GAGCGGTCGA GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG GAGGACGACT TCGCCGGTGT GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG GACCAGGTGG TGCCGGACAA CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG TACGCCGAGT GGTCGGAGGT CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG ACCGAGATCG GCGAGCAGCC GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC TGCGTGCACT TCGTGGCCGA GGAGCAGGAC TGACACGTGC TACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAA TGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC
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