pCAMBIAsuper1300-EYFP-NLS
$84.00
In stock
SKU
PL21721
-
100μg liquid plasmid(Instantly):$105
| name | pCAMBIAsuper1300-EYFP-NLS | another name | apCAMBIAsuper1300-EYFP-NLS |
| Insert Size (bp) | 761bp | Plasmid host | Plant |
| Plasmid uses | Subcellular localization | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Hyg | Fluorescent labeling | Yellow |
| Promoter | MAS | Replicon | pVS1 |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pCAMBIAsuper1300-GFP | 5′ sequencing primer | |
| 3′ sequencing primer | NOS-R | Copy number | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGCTGCTCTA GCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGCC ACGGTATCGA TAAGCTCGCG GATCCCTGAA AGCGACGTTG GATGTTAACA TCTACAAATT GCCTTTTCTT ATCGACCATG TACGTAAGCG CTTACGTTTT TGGTGGACCC TTGAGGAAAC TGGTAGCTGT TGTGGGCCTG TGGTCTCAAG ATGGATCATT AATTTCCACC TTCACCTACG ATGGGGGGCA TCGCACCGGT GAGTAATATT GTACGGCTAA GAGCGAATTT GGCCTGTAGG ATCCCTGAAA GCGACGTTGG ATGTTAACAT CTACAAATTG CCTTTTCTTA TCGACCATGT ACGTAAGCGC TTACGTTTTT GGTGGACCCT TGAGGAAACT GGTAGCTGTT GTGGGCCTGT GGTCTCAAGA TGGATCATTA ATTTCCACCT TCACCTACGA TGGGGGGCAT CGCACCGGTG AGTAATATTG TACGGCTAAG AGCGAATTTG GCCTGTAGGA TCCCTGAAAG CGACGTTGGA TGTTAACATC TACAAATTGC CTTTTCTTAT CGACCATGTA CGTAAGCGCT TACGTTTTTG GTGGACCCTT GAGGAAACTG GTAGCTGTTG TGGGCCTGTG GTCTCAAGAT GGATCATTAA TTTCCACCTT CACCTACGAT GGGGGGCATC GCACCGGTGA GTAATATTGT ACGGCTAAGA GCGAATTTGG CCTGTAGGAT CCGCGAGCTG GTCAATCCCA TTGCTTTTGA AGCAGCTCAA CATTGATCTC TTTCTCGATC GAGGGAGATT TTTCAAATCA GTGCGCAAGA CGTGACGTAA GTATCCGAGT CAGTTTTTAT TTTTCTACTA ATTTGGTCGT TTATTTCGGC GTGTAGGACA TGGCAACCGG GCCTGAATTT CGCGGGTATT CTGTTTCTAT TCCAACTTTT TCTTGATCCG CAGCCATTAA CGACTTTTGA ATAGATACGC TGACACGCCA AGCCTCGCTA GTCAAAAGTG TACCAAACAA CGCTTTACAG CAAGAACGGA ATGCGCGTGA CGCTCGCGGT GACGCCATTT CGCCTTTTCA GAAATGGATA AATAGCCTTG CTTCCTATTA TATCTTCCCA AATTACCAAT ACATTACACT AGCATCTGAA TTTCATAACC AATCTCGATA CACCAAATCG ACTCTAGAAA GCTTCTGCAG GGGCCCGGGG TCGACATTTA AATACTAGTG GATCCGGTAC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCTTCGGCT ACGGCCTGCA GTGCTTCGCC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCAGAA CGTCTATCTG ATGGCCGACA AGCAGAAGAA CGGCATCAAG GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC TACCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGGT ACCCGGGATC CAGCCTAAGA AGAAGAGAAA GGTTGGAGGA TAGAGCTCGA ATTTCCCCGA TCGTTCAAAC ATTTGGCAAT AAAGTTTCTT AAGATTGAAT CCTGTTGCCG GTCTTGCGAT GATTATCATA TAATTTCTGT TGAATTACGT TAAGCATGTA ATAATTAACA TGTAATGCAT GACGTTATTT ATGAGATGGG TTTTTATGAT TAGAGTCCCG CAATTATACA TTTAATACGC GATAGAAAAC AAAATATAGC GCGCAAACTA GGATAAATTA TCGCGCGCGG TGTCATCTAT GTTACTAGAT CGGGAATTCG TAATCATGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGCTAGAGC AGCTTGCCAA CATGGTGGAG CACGACACTC TCGTCTACTC CAAGAATATC AAAGATACAG TCTCAGAAGA CCAAAGGGCT ATTGAGACTT TTCAACAAAG GGTAATATCG GGAAACCTCC TCGGATTCCA TTGCCCAGCT ATCTGTCACT TCATCAAAAG GACAGTAGAA AAGGAAGGTG GCACCTACAA ATGCCATCAT TGCGATAAAG GAAAGGCTAT CGTTCAAGAT GCCTCTGCCG ACAGTGGTCC CAAAGATGGA CCCCCACCCA CGAGGAGCAT CGTGGAAAAA GAAGACGTTC CAACCACGTC TTCAAAGCAA GTGGATTGAT GTGATATCTC CACTGACGTA AGGGATGACG CACAATCCCA CTATCCTTCG CAAGACCCTT CCTCTATATA AGGAAGTTCA TTTCATTTGG AGAGGACACG CTGAAATCAC CAGTCTCTCT CTACAAATCT ATCTCTCTCG AGCTTTCGCA GATCCGGGGG GCAATGAGAT ATGAAAAAGC CTGAACTCAC CGCGACGTCT GTCGAGAAGT TTCTGATCGA AAAGTTCGAC AGCGTCTCCG ACCTGATGCA GCTCTCGGAG GGCGAAGAAT CTCGTGCTTT CAGCTTCGAT GTAGGAGGGC GTGGATATGT CCTGCGGGTA AATAGCTGCG CCGATGGTTT CTACAAAGAT CGTTATGTTT ATCGGCACTT TGCATCGGCC GCGCTCCCGA TTCCGGAAGT GCTTGACATT GGGGAGTTTA GCGAGAGCCT GACCTATTGC ATCTCCCGCC GTTCACAGGG TGTCACGTTG CAAGACCTGC CTGAAACCGA ACTGCCCGCT GTTCTACAAC CGGTCGCGGA GGCTATGGAT GCGATCGCTG CGGCCGATCT TAGCCAGACG AGCGGGTTCG GCCCATTCGG ACCGCAAGGA ATCGGTCAAT ACACTACATG GCGTGATTTC ATATGCGCGA TTGCTGATCC CCATGTGTAT CACTGGCAAA CTGTGATGGA CGACACCGTC AGTGCGTCCG TCGCGCAGGC TCTCGATGAT CTGATGCTTT GGGCCGAGGA CTGCCCCGAA GTCCGGCACC TCGTGCACGC GGATTTCGGC TCCAACAATG TCCTGACGGA CAATGGCCGC ATAACAGCGG TCATTGACTG GAGCGAGGCG ATGTTCGGGG ATTCCCAATA CGAGGTCGCC AACATCTTCT TCTGGAGGCC GTGGTTGGCT TGTATGGAGC AGCAGACGCG CTACTTCGAG CGGAGGCATC CGGAGCTTGC AGGATCGCCA CGACTCCGGG CGTATATGCT CCGCATTGGT CTTGACCAAC TCTATCAGAG CTTGGTTGAC GGCAATTTCG ATGATGCAGC TTGGGCGCAG GGTCGATGCG ACGCAATCGT CCGATCCGGA GCCGGGACTG TCGGGCGTAC ACAAATCGCC CGCAGAAGCG CGGCCGTCTG GACCGATGGC TGTGTAGAAG TACTCGCCGA TAGTGGAAAC CGACGCCCCA GCACTCGTCC GAGGGCAAAG AAATAGAGTA GATGCCGACC GGGATCTGTC GATCGACAAG CTCGAGTTTC TCCATAATAA TGTGTGAGTA GTTCCCAGAT AAGGGAATTA GGGTTCCTAT AGGGTTTCGC TCATGTGTTG AGCATATAAG AAACCCTTAG TATGTATTTG TATTTGTAAA ATACTTCTAT CAATAAAATT TCTAATTCCT AAAACCAAAA TCCAGTACTA AAATCCAGAT CCCCCGAATT AATTCGGCGT TAATTCAGTA CATTAAAAAC GTCCGCAATG TGTTATTAAG TTGTCTAAGC GTCAATTTGT TTACACCACA ATATATCCTG CCACCAGCCA GCCAACAGCT CCCCGACCGG CAGCTCGGCA CAAAATCACC ACTCGATACA GGCAGCCCAT CAGTCCGGGA CGGCGTCAGC GGGAGAGCCG TTGTAAGGCG GCAGACTTTG CTCATGTTAC CGATGCTATT CGGAAGAACG GCAACTAAGC TGCCGGGTTT GAAACACGGA TGATCTCGCG GAGGGTAGCA TGTTGATTGT AACGATGACA GAGCGTTGCT GCCTGTGATC ACCGCGGTTT CAAAATCGGC TCCGTCGATA CTATGTTATA CGCCAACTTT GAAAACAACT TTGAAAAAGC TGTTTTCTGG TATTTAAGGT TTTAGAATGC AAGGAACAGT GAATTGGAGT TCGTCTTGTT ATAATTAGCT TCTTGGGGTA TCTTTAAATA CTGTAGAAAA GAGGAAGGAA ATAATAAATG GCTAAAATGA GAATATCACC GGAATTGAAA AAACTGATCG AAAAATACCG CTGCGTAAAA GATACGGAAG GAATGTCTCC TGCTAAGGTA TATAAGCTGG TGGGAGAAAA TGAAAACCTA TATTTAAAAA TGACGGACAG CCGGTATAAA GGGACCACCT ATGATGTGGA ACGGGAAAAG GACATGATGC TATGGCTGGA AGGAAAGCTG CCTGTTCCAA AGGTCCTGCA CTTTGAACGG CATGATGGCT GGAGCAATCT GCTCATGAGT GAGGCCGATG GCGTCCTTTG CTCGGAAGAG TATGAAGATG AACAAAGCCC TGAAAAGATT ATCGAGCTGT ATGCGGAGTG CATCAGGCTC TTTCACTCCA TCGACATATC GGATTGTCCC TATACGAATA GCTTAGACAG CCGCTTAGCC GAATTGGATT ACTTACTGAA TAACGATCTG GCCGATGTGG ATTGCGAAAA CTGGGAAGAA GACACTCCAT TTAAAGATCC GCGCGAGCTG TATGATTTTT TAAAGACGGA AAAGCCCGAA GAGGAACTTG TCTTTTCCCA CGGCGACCTG GGAGACAGCA ACATCTTTGT GAAAGATGGC AAAGTAAGTG GCTTTATTGA TCTTGGGAGA AGCGGCAGGG CGGACAAGTG GTATGACATT GCCTTCTGCG TCCGGTCGAT CAGGGAGGAT ATCGGGGAAG AACAGTATGT CGAGCTATTT TTTGACTTAC TGGGGATCAA GCCTGATTGG GAGAAAATAA AATATTATAT TTTACTGGAT GAATTGTTTT AGTACCTAGA ATGCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGGGTGCC TTGATGTGGG CGCCGGCGGT CGAGTGGCGA CGGCGCGGCT TGTCCGCGCC CTGGTAGATT GCCTGGCCGT AGGCCAGCCA TTTTTGAGCG GCCAGCGGCC GCGATAGGCC GACGCGAAGC GGCGGGGCGT AGGGAGCGCA GCGACCGAAG GGTAGGCGCT TTTTGCAGCT CTTCGGCTGT GCGCTGGCCA GACAGTTATG CACAGGCCAG GCGGGTTTTA AGAGTTTTAA TAAGTTTTAA AGAGTTTTAG GCGGAAAAAT CGCCTTTTTT CTCTTTTATA TCAGTCACTT ACATGTGTGA CCGGTTCCCA ATGTACGGCT TTGGGTTCCC AATGTACGGG TTCCGGTTCC CAATGTACGG CTTTGGGTTC CCAATGTACG TGCTATCCAC AGGAAAGAGA CCTTTTCGAC CTTTTTCCCC TGCTAGGGCA ATTTGCCCTA GCATCTGCTC CGTACATTAG GAACCGGCGG ATGCTTCGCC CTCGATCAGG TTGCGGTAGC GCATGACTAG GATCGGGCCA GCCTGCCCCG CCTCCTCCTT CAAATCGTAC TCCGGCAGGT CATTTGACCC GATCAGCTTG CGCACGGTGA AACAGAACTT CTTGAACTCT CCGGCGCTGC CACTGCGTTC GTAGATCGTC TTGAACAACC ATCTGGCTTC TGCCTTGCCT GCGGCGCGGC GTGCCAGGCG GTAGAGAAAA CGGCCGATGC CGGGATCGAT CAAAAAGTAA TCGGGGTGAA CCGTCAGCAC GTCCGGGTTC TTGCCTTCTG TGATCTCGCG GTACATCCAA TCAGCTAGCT CGATCTCGAT GTACTCCGGC CGCCCGGTTT CGCTCTTTAC GATCTTGTAG CGGCTAATCA AGGCTTCACC CTCGGATACC GTCACCAGGC GGCCGTTCTT GGCCTTCTTC GTACGCTGCA TGGCAACGTG CGTGGTGTTT AACCGAATGC AGGTTTCTAC CAGGTCGTCT TTCTGCTTTC CGCCATCGGC TCGCCGGCAG AACTTGAGTA CGTCCGCAAC GTGTGGACGG AACACGCGGC CGGGCTTGTC TCCCTTCCCT TCCCGGTATC GGTTCATGGA TTCGGTTAGA TGGGAAACCG CCATCAGTAC CAGGTCGTAA TCCCACACAC TGGCCATGCC GGCCGGCCCT GCGGAAACCT CTACGTGCCC GTCTGGAAGC TCGTAGCGGA TCACCTCGCC AGCTCGTCGG TCACGCTTCG ACAGACGGAA AACGGCCACG TCCATGATGC TGCGACTATC GCGGGTGCCC ACGTCATAGA GCATCGGAAC GAAAAAATCT GGTTGCTCGT CGCCCTTGGG CGGCTTCATA ATCGACGGCG CACCGGCTGC CGGCGGTTGC CGGGATTCTT TGCGGATTCG ATCAGCGGCC GCTTGCCACG ATTCACCGGG GCGTGCTTCT GCCTCGATGC GTTGCCGCTG GGCGGCCTGC GCGGCCTTCA ACTTCTCCAC CAGGTCATCA CCCAGCGCCG CGCCGATTTG TACCGGGCCG GATGGTTTGC GACCGCTCAC GCCGATTCCT CGGGCTTGGG GGTTCCAGTG CCATTGCAGG GCCGGCAGGC AACCCAGCCG CTTACGCCTG GCCAACCGCC CGTTCCTCCA CACATGGGGC ATTCCACGGC GTCGGTGCCT GGTTGTTCTT GATTTTCCAT GCCGCCTCCT TTAGCCGCTA AAATTCATCT ACTCATTTAT TCATTTGCTC ATTTACTCTG GTAGCTGCGC GATGTATTCA GATAGCAGCT CGGTAATGGT CTTGCCTTGG CGTACCGCGT ACATCTTCAG CTTGGTGTGA TCCTCCGCCG GCAACTGAAA GTTGACCCGC TTCATGGCTG GCGTGTCTGC CAGGCTGGCC AACGTTGCAG CCTTGCTGCT GCGTGCGCTC GGACGGCCGG CACTTAGCGT GTTTGTGCTT TTGCTCATTT TCTCTTTACC TCATTAACTC AAATGAGTTT TGATTTAATT TCAGCGGCCA GCGCCTGGAC CTCGCGGGCA GCGTCGCCCT CGGGTTCTGA TTCAAGAACG GTTGTGCCGG CGGCGGCAGT GCCTGGGTAG CTCACGCGCT GCGTGATACG GGACTCAAGA ATGGGCAGCT CGTACCCGGC CAGCGCCTCG GCAACCTCAC CGCCGATGCG CGTGCCTTTG ATCGCCCGCG ACACGACAAA GGCCGCTTGT AGCCTTCCAT CCGTGACCTC AATGCGCTGC TTAACCAGCT CCACCAGGTC GGCGGTGGCC CATATGTCGT AAGGGCTTGG CTGCACCGGA ATCAGCACGA AGTCGGCTGC CTTGATCGCG GACACAGCCA AGTCCGCCGC CTGGGGCGCT CCGTCGATCA CTACGAAGTC GCGCCGGCCG ATGGCCTTCA CGTCGCGGTC AATCGTCGGG CGGTCGATGC CGACAACGGT TAGCGGTTGA TCTTCCCGCA CGGCCGCCCA ATCGCGGGCA CTGCCCTGGG GATCGGAATC GACTAACAGA ACATCGGCCC CGGCGAGTTG CAGGGCGCGG GCTAGATGGG TTGCGATGGT CGTCTTGCCT GACCCGCCTT TCTGGTTAAG TACAGCGATA ACCTTCATGC GTTCCCCTTG CGTATTTGTT TATTTACTCA TCGCATCATA TACGCAGCGA CCGCATGACG CAAGCTGTTT TACTCAAATA CACATCACCT TTTTAGACGG CGGCGCTCGG TTTCTTCAGC GGCCAAGCTG GCCGGCCAGG CCGCCAGCTT GGCATCAGAC AAACCGGCCA GGATTTCATG CAGCCGCACG GTTGAGACGT GCGCGGGCGG CTCGAACACG TACCCGGCCG CGATCATCTC CGCCTCGATC TCTTCGGTAA TGAAAAACGG TTCGTCCTGG CCGTCCTGGT GCGGTTTCAT GCTTGTTCCT CTTGGCGTTC ATTCTCGGCG GCCGCCAGGG CGTCGGCCTC GGTCAATGCG TCCTCACGGA AGGCACCGCG CCGCCTGGCC TCGGTGGGCG TCACTTCCTC GCTGCGCTCA AGTGCGCGGT ACAGGGTCGA GCGATGCACG CCAAGCAGTG CAGCCGCCTC TTTCACGGTG CGGCCTTCCT GGTCGATCAG CTCGCGGGCG TGCGCGATCT GTGCCGGGGT GAGGGTAGGG CGGGGGCCAA ACTTCACGCC TCGGGCCTTG GCGGCCTCGC GCCCGCTCCG GGTGCGGTCG ATGATTAGGG AACGCTCGAA CTCGGCAATG CCGGCGAACA CGGTCAACAC CATGCGGCCG GCCGGCGTGG TGGTGTCGGC CCACGGCTCT GCCAGGCTAC GCAGGCCCGC GCCGGCCTCC TGGATGCGCT CGGCAATGTC CAGTAGGTCG CGGGTGCTGC GGGCCAGGCG GTCTAGCCTG GTCACTGTCA CAACGTCGCC AGGGCGTAGG TGGTCAAGCA TCCTGGCCAG CTCCGGGCGG TCGCGCCTGG TGCCGGTGAT CTTCTCGGAA AACAGCTTGG TGCAGCCGGC CGCGTGCAGT TCGGCCCGTT GGTTGGTCAA GTCCTGGTCG TCGGTGCTGA CGCGGGCATA GCCCAGCAGG CCAGCGGCGG CGCTCTTGTT CATGGCGTAA TGTCTCCGGT TCTAGTCGCA AGTATTCTAC TTTATGCGAC TAAAACACGC GACAAGAAAA CGCCAGGAAA AGGGCAGGGC GGCAGCCTGT CGCGTAACTT AGGACTTGTG CGACATGTCG TTTTCAGAAG ACGGCTGCAC TGAACGTCAG AAGCCGACTG CACTATAGCA GCGGAGGGGT TGGATCAAAG TACTTTGATC CCGAGGGGAA CCCTGTGGTT GGCATGCACA TACAAATGGA CGAACGGATA AACCTTTTCA CGCCCTTTTA AATATCCGAT TATTCTAATA AACGCTCTTT TCTCTTAGGT TTACCCGCCA ATATATCCTG TCAAACACTG ATAGTTTAAA CTGAAGGCGG GAAACGACAA TCTGATCCAA GCTCAAGCTG CTCTAGCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGG
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