pLV3-CMV-Kdm5c(mouse)-Puro
$113.00
In stock
SKU
PL21574
NM_013668.5;D930009K15Rik; Jarid1c; mKIAA0234; Smcx
100μg liquid plasmid(Instantly):$134
| name | pLV3-CMV-Kdm5c(mouse)-Puro | another name | apLV3-CMV-Kdm5c(mouse)-Puro |
| Insert Size (bp) | 4656bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | pLV3-CMV-MCS-Puro | 5′ sequencing primer | CMV-F |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT AGAGCTAGCG AATTCGCCAC CATGGAGCTG GGGTCCGACG ATTTCCTACC GCCGCCGGAG TGTCCGGTGT TCGAGCCTAG CTGGGCCGAG TTCCGAGACC CTCTTGGCTA CATCGCGAAA ATCAGACCCA TCGCCGAGAA GTCCGGCATT TGCAAGATCC GCCCACCCGC GGACTGGCAG CCACCCTTTG CCGTGGAAGT GGATAACTTC AGGTTTACTC CCCGAATCCA GAGGTTGAAT GAGCTGGAGG CCCAGACAAG AGTGAAACTG AACTATTTGG ACCAGATTGC CAAATTCTGG GAGATCCAGG GCTCATCCTT AAAGATTCCC AATGTAGAAC GGCGGATCTT GGACCTCTAT AGCCTCAGCA AAATTGTGGT GGAAGAAGGT GGTTATGAAA CTATCTGCAA GGACCGTCGA TGGGCCCGTG TGGCCCAGCG TCTCAACTAC CCACCTGGCA AAAACATTGG TTCCTTGCTA CGCTCTCACT ATGAACGCAT TGTTTATCCC TATGAAATGT ACCAGTCTGG AGCCAACCTT GTGCAGTGTA ACACACGCCC ATTTGATAAT GAGGAAAAGG ACAAGGAATA TAAACCCCAC AGCATCCCCC TTCGACAGTC TGTGCAGCCT TCCAAGTTCA ACAGTTATGG ACGACGGGCA AAGAGACTAC AGCCTGATCC GGAACCCACA GAGGAAGACA TTGAAAAGAA TCCAGAACTG AAGAAATTAC AGATATATGG AGCAGGCCCC AAGATGATGG GGCTGGGCCT AATGGCCAAA GATAAGACTC TGCGGAAAAA AGATAAAGAA GGGCCTGAGT GTCCCCCCAC TGTGGTGGTA AAGGAGGAAT TAGGTGGGGA TGTCAAGATG GAATCAACCT CACCCAAGAC CTTCCTAGAA GGCAAGGAGG AACTGAGTCA TAGCCCTGAG CCCTGCACCA AGATGACCAT GAGGCTTCGA AGGAACCACA GCAATGCCCA GTTTATTGAG TCATACGTAT GCCGAATGTG TTCCCGAGGA GATGAAGATG ACAAACTCTT ACTGTGTGAT GGCTGTGATG ACAATTACCA CATCTTTTGC CTGCTGCCTC CTTTGCCTGA AATCCCGAAA GGTGTCTGGA GGTGTCCAAA GTGTGTCATG GCGGAGTGTA AGCGGCCCCC TGAAGCCTTT GGCTTTGAGC AAGCTACCCG GGAATATACT CTGCAGAGCT TTGGAGAGAT GGCTGACTCC TTTAAAGCTG ACTACTTCAA CATGCCTGTG CATATGGTAC CCACAGAACT TGTAGAGAAG GAGTTTTGGC GGCTGGTGAA TAGCATCGAG GAAGACGTAA CTGTGGAGTA TGGGGCTGAC ATCCATTCCA AAGAATTTGG CAGCGGTTTC CCTGTCAGTG ACAGTAAGCG GCACCTAACC CCAGAGGAGG AGGAGTATGC TACAAGTGGT TGGAACCTGA ATGTGATGCC TGTATTGGAG CAGTCTGTAC TGTGCCACAT CAATGCAGAT ATATCTGGCA TGAAGGTACC GTGGCTGTAT GTGGGAATGG TCTTCTCAGC CTTTTGCTGG CATATTGAAG ATCACTGGAG TTACTCTATT AACTACCTCC ACTGGGGTGA GCCAAAAACC TGGTATGGAG TCCCATCGCT TGCAGCGGAA CACTTGGAAG AAGTGATGAA GAAGCTGACA CCTGAGCTTT TTGATAGCCA GCCTGATCTC CTTCACCAAC TCGTCACCCT CATGAATCCT AACACGCTCA TGTCCCATGG TGTGCCGGTT GTCCGCACAA ACCAGTGTGC AGGAGAATTT GTCATCACTT TTCCTCGTGC TTACCACAGT GGTTTCAACC AAGGCTACAA CTTTGCTGAG GCAGTCAACT TTTGCACTGC TGACTGGCTG CCTGCTGGGC GCCAATGCAT TGAGCACTAC CGCCGACTAC GAAGATACTG TGTCTTTTCT CATGAGGAGC TCATTTGCAA GATGGCTGCC TGCCCTGAGA AACTGGACCT GAACCTGGCA GCAGCTGTAC ATAAGGAGAT GTTCATTATG GTGCAAGAGG AGCGGCGTCT ACGAAAGGCT CTGTTGGAAA AAGGTATCAC CGAGGCTGAG CGAGAGGCTT TTGAGCTGCT TCCAGATGAT GAACGTCAGT GCATAAAGTG CAAGACCACA TGTTTCCTAT CTGCCCTGGC TTGCTATGAC TGCCCAGATG GCCTTGTCTG CCTTTCTCAC ATCAATGACC TCTGCAAGTG CTCCAGTAGC CGCCAGTACC TTCGGTATCG GTACACCTTG GATGAGCTCC CTGCCATGCT GCATAAACTG AAAGTTAGAG CTGAGTCCTT TGACACCTGG GCTAACAAAG TACGAGTGGC CCTGGAGGTG GAAGATGGGC GGAAGCGCAG CCTGGAAGAA CTGAGAGCAC TAGAATCTGA GGCTCGTGAG CGAAGGTTTC CTAACAGTGA GCTTCTACAG CGACTGAAAA ACTGCCTGAG TGAAGCAGAG GCTTGTGTGT CCCGGGCTCT GGGATTGGTC AGTGGCCAGG AAGCTGGCCC TGACAGGGTG GCTGGCCTAC AGATGACCCT GGCTGAGCTA CGGGATTTTC TGGGCCAGAT GAACAACCTG CCCTGTGCCA TGCACCAGAT TGGGGATGTC AAGGGTATTC TGGAACAGGT GGAAGCCTAC CAGACTGAGG CCCGTGAGGC CCTGGTCTCA CAGCCTTCCA GTCCAGGGCT GCTGCAGTCC TTGTTGGAGA GAGGACAACA GCTGGGTGTG GAGGTACCTG AAGCCCAGCA GCTCCAAAGG CAGGTGGAAC AGGCGCGGTG GCTGGATGAG GTAAAACGCA CGCTGGCTCC CTCTGCCCGA AGGGGTACCC TGGCCATCAT GCGGGGACTG TTGGTTGCGG GCGCCAGTGT AGCCCCTAGC CCTGCTGTGG ATAAGGCCCA GGCAGAGCTT CAGGAGCTGC TGACCATCGC TGAACGCTGG GAAGAGAAAG CCCACCTCTG CTTAGAGGCC AGGCAGAAGC ATCCACCAGC CACACTTGAG GCCATAATCC ATGAAGCAGA AAACATCCCT GTTCACTTGC CCAATATCCA GTCTCTCAAG GAGGCTCTTG CTAAGGCCCG GGCATGGATT GCTGATGTGG ATGAGATCCA AAATGGTGAC CACTACCCTT GCTTGGATGA CTTGGAGGGC CTCGTAGCTG TGGGTCGGGA CCTCCCTGTG GGGCTGGAGG AACTGAGACA GCTAGAGCTA CAGGTATTGA CAGCACACTC CTGGAGAGAA AAGGCCTCCA AGACCTTCCT CAAGAAGAAC TCCTGCTATA CATTATTGGA GGTGCTCTGC CCGTGTGCAG ACGCCGGCTC AGACAGCACC AAACGCAGCC GGTGGATGGA GAAGGAGCTG GGGTTGTACA AATCTGACAC AGAGCTGTTG GGGCTGTCTG CGCAGGACCT CAGGGACCCA GGCTCTGTGA TCGTGGCCTT CAAAGAGGGG GAGCAGAAGG AGAAGGAAGG GATACTGCAG CTTCGTCGTA CCAACTCAGC CAAGCCAAGT CCACTGGCAC TGTTGACAAC AGCCTCCTCT ACAGCTTCTA TCTGCGTGTG TGGGCAAGTG CCAGCTGGGG TGGGAGCTCT GCAGTGTGAC CTGTGTCAGG ACTGGTTCCA TGGGCGCTGT GTGACAGTAC CCCGCCTCCT CAGCTCCCAG AGGTCCAGTC TTCCCTCATC CCCACTGCTG GCCTGGTGGG AATGGGACAC CAAATTCTTG TGCCCTCTGT GTATGCGCTC ACGGCGCCCA CGCTTGGAAA CCATCCTGGC ACTGCTGGTA GCCCTACAGA GACTGCCTGT GCGGCTGCCT GAGGGTGAGG CTCTACAGTG CCTCACAGAG AGAGCCATCA GCTGGCAAGG CCGTGCCAGA CAGGTTTTGG CCTCTGAGGA AGTGACTGCT CTGTTGGGAC GGCTGGCTGA ACTGCGCCAA CGGCTACAGG CTGAATCCAA GCCTGAGGAG TCCCTGGCTT ACCCTTCAGA TGGTGGAGAG GGCACTGGCA ATATGCCGAA GGTCCAGGGG CTGTTGGAGA ATGGGGACAG TGTGACCAGT CCTGAAAAGG TAGCCACAGA GGAGGGCTCA GATCTGGAGC TGCTATCCTC AATATTACCA CAGTTGTCTG GCCCTGTGTT GGAACTGCCT GAGGCAACCC GAGCCCCACT GGAGGAACTT ATGATGGAAG GGGATCTGCT TGAGGTGACC CTAGATGAGA ATCATAGCAT CTGGCAGCTG CTGCAGGCTG GGCAGCCTCC AGACTTGAAG AGGGTCCAGA CACTTCTGGA GCTGGAGAAG GCAGAGCGCC ATGGGAGTCG GACACGAGGC CGAGCCCTAG AGAGGAGACG ACGGAGGAAG GTGGATCGGG GTGGGGAGCC GGATGACCCG GCACGAGAGG AGCTAGAGCC AAAGAGGGTA CGAAGCTCAG GACCAGAAGC CGAGGAAGTC CAGGAGGAAG AGGAGCTGGA GGAGGAGACT GGGGGTGAGG TCCCTCCTGT ACCCTTCCCC AACAGTGGCA GCCCCAGCAT CCAGGAGGAC CAGGATGGCT TAGAACCAGT GTTAGAGGCT GGTTCTGACA CCTCAGCCCC TTTCTCTACT CTGACTTCCC GGCTGTTGAT GTCCTGCCCA CAGCAGCCAT CTCTGCAACA ATTGTGAGGA TCCGCGGCCG CGAAGGATCT GCGATCGCTC CGGTGCCCGT CAGTGGGCAG AGCGCACATC GCCCACAGTC CCCGAGAAGT TGGGGGGAGG GGTCGGCAAT TGAACGGGTG CCTAGAGAAG GTGGCGCGGG GTAAACTGGG AAAGTGATGT CGTGTACTGG CTCCGCCTTT TTCCCGAGGG TGGGGGAGAA CCGTATATAA GTGCAGTAGT CGCCGTGAAC GTTCTTTTTC GCAACGGGTT TGCCGCCAGA ACACAGCTGA AGCTTCGAGG GGCTCGCATC TCTCCTTCAC GCGCCCGCCG CCCTACCTGA GGCCGCCATC CACGCCGGTT GAGTCGCGTT CTGCCGCCTC CCGCCTGTGG TGCCTCCTGA ACTGCGTCCG CCGTCTAGGT AAGTTTAAAG CTCAGGTCGA GACCGGGCCT TTGTCCGGCG CTCCCTTGGA GCCTACCTAG ACTCAGCCGG CTCTCCACGC TTTGCCTGAC CCTGCTTGCT CAACTCTACG TCTTTGTTTC GTTTTCTGTT CTGCGCCGTT ACAGATCCAA GCTGTGACCG GCGCCTACGC TAGATGACCG AGTACAAGCC CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCCGGGCC GTACGCACCC TCGCCGCCGC GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGACCCG GACCGCCACA TCGAGCGGGT CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGAGTCGACA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAATCATC GTCCTTTCCT TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC CTCCCCGCCT GGTACCTTTA AGACCAATGA CTTACAAGGC AGCTGTAGAT CTTAGCCACT TTTTAAAAGA AAAGGGGGGA CTGGAAGGGC TAATTCACTC CCAACGAAAA TAAGATCTGC TTTTTGCTTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGTAGT AGTTCATGTC ATCTTATTAT TCAGTATTTA TAACTTGCAA AGAAATGAAT ATCAGAGAGT GAGAGGAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGGCT CTAGCTATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGACTTT TGCAGAGACG GCCCAAATTC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC GTATCACGAG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGCCA AGCTG
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