pLV3-CMV-CR1(human-repeat-opt)-3×FLAG-Puro
100μg liquid plasmid(Instantly):$178
| name | pLV3-CMV-CR1(human-repeat-opt)-3×FLAG-Puro | another name | apLV3-CMV-CR1(human-repeat-opt)-3×FLAG-Puro |
| Insert Size (bp) | 7467bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | pLV3-CMV-MCS-3×FLAG-Puro | 5′ sequencing primer | CMV-F |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT AGAGCTAGCG AATTCGCCAC CATGGGCGCA TCCTCACCCA GGTCTCCAGA ACCAGTGGGC CCTCCAGCAC CAGGCCTTCC TTTCTGCTGT GGTGGGAGCC TTTTGGCCGT GGTAGTTCTG CTGGCCCTGC CAGTGGCGTG GGGACAATGC AACGCCCCCG AATGGCTCCC ATTTGCAAGG CCTACCAACC TGACAGACGA GTTTGAGTTC CCAATTGGGA CCTATCTGAA CTACGAATGT AGGCCAGGGT ACTCTGGCAG ACCTTTTAGC ATCATCTGTT TGAAAAATAG CGTGTGGACC GGCGCTAAAG ACCGCTGTAG GCGAAAATCT TGCAGAAATC CACCGGACCC TGTCAACGGT ATGGTGCACG TTATCAAGGG CATCCAGTTT GGTAGCCAGA TCAAGTACTC CTGCACAAAG GGCTATCGGC TGATCGGAAG CAGTTCTGCC ACTTGCATCA TCTCCGGAGA CACCGTGATC TGGGACAACG AGACCCCCAT ATGCGACAGG ATTCCTTGTG GCCTGCCACC TACTATCACT AACGGCGACT TCATCTCCAC GAATAGAGAG AATTTTCATT ACGGATCTGT TGTCACGTAT CGCTGCAACC CGGGGAGTGG AGGGAGAAAG GTGTTTGAAC TGGTCGGCGA GCCTAGCATT TACTGCACCT CAAACGATGA TCAGGTCGGA ATCTGGTCAG GACCCGCCCC CCAGTGTATA ATCCCCAACA AGTGCACACC ACCAAACGTA GAAAATGGGA TCCTGGTATC AGATAATAGG TCTTTGTTTA GCCTGAATGA GGTTGTCGAG TTTAGGTGCC AGCCTGGGTT TGTTATGAAA GGTCCGCGGA GGGTGAAGTG CCAGGCACTT AATAAGTGGG AACCGGAACT TCCCTCCTGC AGTCGCGTCT GTCAACCCCC TCCCGACGTA TTGCACGCTG AAAGAACCCA GAGGGACAAG GACAATTTTT CCCCTGGTCA GGAGGTGTTC TACAGTTGTG AACCCGGGTA CGACCTCAGG GGAGCTGCTA GTATGCGCTG TACTCCACAG GGAGATTGGA GTCCAGCCGC ACCAACATGT GAAGTGAAAA GCTGTGACGA TTTCATGGGG CAGCTCCTGA ACGGCCGCGT GCTGTTCCCC GTGAACTTGC AGTTGGGGGC TAAGGTCGAC TTCGTGTGTG ATGAGGGATT TCAATTGAAG GGCTCCTCAG CTTCCTACTG CGTGTTGGCG GGGATGGAAA GCCTGTGGAA TTCAAGTGTC CCTGTGTGCG AGCAAATCTT CTGCCCATCT CCACCCGTCA TTCCTAATGG GCGGCATACC GGGAAGCCAC TTGAGGTGTT TCCCTTTGGG AAGACGGTAA ATTACACGTG TGACCCACAC CCGGATAGAG GTACCTCTTT TGACTTGATT GGCGAGTCTA CCATTAGGTG CACCTCAGAT CCCCAAGGAA ATGGTGTGTG GAGCAGCCCC GCTCCTAGAT GCGGTATTCT GGGCCACTGC CAAGCTCCTG ACCACTTCCT GTTTGCTAAA TTGAAAACAC AGACCAATGC CTCCGACTTC CCGATTGGCA CCTCCCTGAA ATATGAGTGC AGGCCGGAGT ACTATGGCCG GCCTTTCTCC ATAACATGTC TGGATAACCT TGTCTGGTCT AGTCCGAAGG ATGTGTGCAA AAGAAAGTCC TGTAAGACCC CACCAGATCC CGTGAACGGC ATGGTTCACG TCATAACCGA CATCCAGGTG GGCAGCAGAA TTAACTACTC CTGTACCACC GGCCATCGGC TGATCGGCCA TTCTTCAGCT GAGTGTATCC TTAGCGGCAA TGCTGCTCAC TGGAGCACCA AGCCACCCAT TTGTCAGCGC ATTCCCTGTG GGCTGCCACC CACAATTGCC AATGGCGACT TTATCTCCAC CAATCGAGAG AATTTCCATT ACGGATCCGT CGTGACATAC AGATGCAACC CCGGGTCTGG GGGGAGAAAG GTCTTCGAGC TGGTGGGGGA ACCAAGTATT TATTGCACTT CCAATGACGA CCAGGTGGGA ATCTGGAGTG GGCCAGCCCC TCAGTGTATC ATCCCCAATA AGTGCACACC CCCAAATGTT GAGAACGGAA TCCTCGTGTC TGATAACAGG AGCTTGTTTA GCCTCAACGA GGTCGTTGAG TTCCGCTGCC AGCCTGGGTT CGTTATGAAG GGACCACGCC GGGTCAAATG CCAGGCCCTG AATAAGTGGG AACCCGAGCT GCCTAGCTGC TCCCGCGTGT GTCAGCCTCC TCCAGACGTG CTGCACGCTG AACGGACCCA GAGAGATAAA GACAACTTCT CCCCCGGGCA GGAGGTGTTT TATAGCTGTG AGCCTGGATA TGACCTGAGG GGCGCGGCCT CCATGAGATG TACTCCTCAG GGCGATTGGA GCCCTGCGGC ACCAACATGC GAAGTGAAGA GTTGCGACGA TTTCATGGGC CAGCTTTTGA ATGGCCGGGT GCTCTTCCCC GTCAATCTCC AACTTGGTGC TAAGGTGGAC TTCGTGTGCG ATGAAGGATT TCAGTTGAAA GGCAGCAGTG CCAGTTACTG CGTACTGGCC GGCATGGAAA GCCTTTGGAA TTCCTCAGTG CCAGTCTGTG AGCAGATTTT CTGTCCCAGC CCACCTGTGA TTCCCAACGG ACGCCACACC GGTAAACCCC TGGAAGTCTT CCCTTTCGGT AAAACGGTCA ATTACACCTG TGACCCGCAT CCCGATAGGG GCACCTCCTT CGACCTTATA GGTGAGAGCA CTATTAGATG CACCTCAGAT CCTCAGGGCA ATGGTGTGTG GAGTAGTCCC GCTCCTCGCT GCGGCATTCT TGGACATTGT CAGGCCCCTG ACCATTTTCT CTTCGCCAAG CTTAAAACTC AAACGAATGC GAGCGACTTT CCGATTGGAA CGTCTCTGAA ATACGAATGC CGACCCGAGT ATTATGGACG GCCATTCAGT ATCACCTGTC TGGACAACCT TGTATGGAGT TCTCCAAAGG ACGTATGTAA GAGGAAGAGC TGTAAGACTC CCCCAGACCC CGTAAATGGC ATGGTACACG TGATCACCGA CATTCAAGTG GGGTCCCGGA TCAATTACTC TTGCACCACA GGACATAGAC TGATCGGCCA CAGTTCCGCT GAGTGCATCC TCTCAGGCAA TGCTGCACAC TGGTCTACCA AACCTCCAAT CTGCCAGAGG ATTCCATGCG GACTCCCGCC TACCATAGCA AACGGTGACT TTATATCAAC AAACAGGGAG AACTTTCACT ACGGGTCTGT CGTGACCTAT AGATGCAACC CTGGTAGTGG TGGTAGAAAG GTTTTCGAGC TGGTGGGAGA ACCTAGTATC TACTGCACCA GTAACGATGA CCAGGTGGGT ATATGGAGCG GGCCCGCACC TCAGTGTATC ATTCCAAATA AGTGCACACC CCCCAACGTG GAAAACGGGA TCCTGGTGTC AGATAACCGC TCCTTGTTTT CCCTGAACGA AGTCGTGGAG TTTCGATGCC AACCCGGCTT CGTAATGAAA GGACCGCGCA GAGTTAAGTG CCAGGCTCTC AATAAGTGGG AGCCTGAGCT GCCAAGCTGC TCCAGGGTGT GCCAACCCCC TCCGGACGTG CTGCATGCAG AGAGAACGCA GAGAGATAAG GATAACTTCT CCCCAGGCCA GGAGGTTTTC TATTCCTGCG AACCAGGATA TGATCTGCGG GGCGCCGCTA GCATGCGCTG TACACCTCAG GGAGACTGGA GTCCTGCTGC CCCAACCTGC GAGGTGAAGA GTTGCGATGA TTTCATGGGC CAGCTCCTGA ATGGCCGGGT TCTGTTTCCG GTAAACTTGC AGCTCGGGGC CAAAGTAGAC TTTGTGTGTG ATGAGGGTTT CCAATTGAAA GGGAGCAGTG CGTCATATTG CGTGTTGGCC GGGATGGAGT CTCTGTGGAA TAGTAGTGTG CCAGTATGTG AGCAAATCTT CTGTCCTAGC CCCCCCGTGA TTCCTAACGG TCGACACACT GGAAAACCAT TGGAAGTGTT TCCATTCGGA AAAGCAGTGA ACTACACGTG TGATCCCCAT CCTGACAGGG GCACATCATT CGATCTGATT GGGGAATCAA CGATTAGATG CACAAGTGAC CCTCAGGGGA ATGGGGTTTG GTCATCTCCC GCCCCACGGT GTGGAATCCT GGGACACTGC CAGGCTCCCG ACCATTTCCT GTTCGCTAAA CTGAAGACAC AGACAAACGC CTCCGATTTT CCCATTGGCA CTAGTCTGAA ATACGAGTGT CGACCAGAAT ACTACGGGAG GCCCTTTAGC ATCACCTGTC TTGATAATCT CGTGTGGAGT TCCCCGAAGG ATGTCTGCAA AAGAAAAAGC TGTAAAACAC CCCCCGACCC CGTGAACGGC ATGGTCCATG TAATCACGGA CATCCAGGTC GGTTCTCGGA TTAACTACAG CTGTACCACA GGTCATAGAC TGATCGGCCA TTCTTCTGCC GAATGCATCT TGTCCGGCAA CACAGCCCAC TGGAGCACCA AACCTCCCAT CTGCCAGAGG ATTCCGTGCG GTCTGCCCCC CACAATTGCC AATGGAGACT TCATCTCTAC CAACAGGGAA AACTTCCATT ACGGCTCTGT TGTTACGTAT AGATGCAATC TCGGAAGCAG AGGGCGGAAG GTTTTCGAAC TTGTGGGAGA ACCCAGCATA TATTGCACGA GTAACGACGA TCAGGTGGGG ATATGGTCCG GACCAGCGCC CCAGTGTATA ATCCCTAACA AATGTACTCC TCCCAACGTC GAGAACGGTA TCTTGGTGAG CGACAACCGG AGCCTGTTTA GCCTCAATGA AGTGGTAGAG TTTAGATGTC AGCCCGGGTT TGTCATGAAG GGGCCTCGAA GGGTCAAATG TCAGGCACTG AACAAATGGG AACCAGAGCT GCCAAGTTGC TCCAGGGTGT GCCAGCCGCC ACCGGAAATT CTGCATGGGG AGCACACCCC TTCACACCAG GACAACTTCT CCCCTGGTCA GGAAGTGTTT TACAGTTGCG AACCGGGATA CGATTTGCGG GGTGCAGCAT CACTCCACTG TACTCCACAG GGCGATTGGT CTCCAGAAGC GCCACGCTGT GCCGTTAAAT CTTGTGACGA CTTTCTTGGA CAGCTGCCCC ATGGGAGAGT TCTGTTTCCC CTGAACCTTC AACTTGGAGC AAAGGTCAGT TTTGTGTGTG ACGAAGGCTT TCGATTGAAG GGCAGTTCAG TGTCCCACTG TGTTCTGGTG GGGATGAGAA GCCTGTGGAA CAATAGCGTT CCTGTTTGTG AACACATTTT TTGCCCTAAT CCCCCAGCTA TCCTTAACGG GAGACACACC GGCACACCGA GCGGCGATAT CCCCTACGGT AAAGAGATTT CTTACACATG CGATCCTCAC CCTGACCGAG GAATGACTTT TAATCTGATA GGCGAGTCCA CAATCAGATG CACTAGCGAC CCACACGGTA ACGGAGTGTG GAGTAGTCCA GCCCCTCGGT GCGAACTGAG CGTTAGAGCC GGTCATTGCA AAACCCCGGA GCAGTTCCCA TTTGCCAGTC CCACCATTCC GATTAATGAC TTCGAGTTTC CAGTTGGCAC CAGTCTGAAT TATGAATGCC GGCCCGGATA CTTCGGAAAG ATGTTCTCCA TTAGCTGCCT GGAGAACTTG GTCTGGAGTT CTGTTGAGGA CAACTGTAGG CGGAAATCTT GTGGACCACC CCCTGAACCC TTCAATGGTA TGGTCCATAT CAACACAGAC ACACAGTTTG GGTCTACTGT CAATTATAGT TGTAATGAAG GGTTTCGGCT CATAGGCAGT CCTAGCACCA CATGTCTCGT TAGCGGCAAC AATGTGACCT GGGACAAAAA GGCTCCCATT TGTGAAATTA TTAGCTGCGA GCCTCCACCA ACGATATCAA ACGGTGATTT TTATAGTAAT AACCGCACAA GTTTTCATAA CGGGACGGTG GTCACCTACC AGTGTCACAC AGGACCTGAC GGGGAACAGC TGTTCGAGCT GGTTGGTGAA CGGAGCATTT ATTGTACATC TAAAGATGAC CAGGTAGGAG TCTGGAGTTC TCCACCGCCC CGCTGTATCT CCACTAACAA GTGCACTGCA CCTGAAGTCG AGAACGCCAT CAGGGTGCCT GGCAACAGGT CCTTTTTCAC TCTGACCGAA ATTATCCGCT TTCGATGTCA GCCGGGATTT GTAATGGTCG GATCCCACAC AGTTCAATGT CAGACAAATG GACGGTGGGG ACCCAAACTG CCTCACTGCA GTCGAGTGTG TCAGCCACCT CCTGAGATAT TGCACGGCGA ACATACTCTG AGTCACCAAG ATAATTTCAG CCCCGGCCAG GAGGTGTTCT ACTCCTGTGA ACCGTCTTAT GATCTCCGAG GTGCTGCATC TTTGCATTGC ACACCCCAAG GAGACTGGTC TCCAGAAGCT CCCCGATGTA CTGTGAAGAG CTGTGACGAT TTTCTTGGCC AGTTGCCGCA CGGGAGAGTG CTGCTGCCCC TTAATCTCCA ATTGGGGGCC AAGGTGTCAT TTGTATGTGA TGAGGGATTC CGCCTGAAGG GAAGGAGCGC TAGCCATTGT GTCCTCGCTG GAATGAAGGC TCTCTGGAAC AGCTCCGTTC CGGTATGTGA GCAGATTTTT TGCCCCAATC CCCCAGCCAT CCTCAATGGT CGGCATACTG GTACGCCATT TGGTGATATT CCATATGGCA AAGAAATCAG CTATGCCTGC GATACGCACC CGGATAGGGG GATGACTTTC AACCTGATTG GTGAGAGCTC CATTAGGTGC ACCAGTGACC CTCAAGGCAA TGGGGTGTGG AGCTCACCTG CACCTAGATG TGAGCTGTCA GTGCCCGCCG CATGTCCTCA CCCTCCTAAA ATCCAGAACG GACACTACAT TGGCGGGCAT GTCAGCCTCT ACCTCCCTGG CATGACAATT TCATATATCT GCGACCCTGG TTATTTGCTT GTCGGCAAGG GCTTTATCTT CTGTACAGAC CAGGGCATTT GGAGCCAGCT CGACCACTAT TGTAAAGAGG TGAATTGCTC TTTTCCTCTG TTTATGAACG GGATTTCTAA GGAGCTGGAG ATGAAGAAGG TGTACCACTA TGGCGACTAC GTTACCCTGA AATGTGAAGA CGGCTACACA CTGGAGGGGT CACCCTGGTC CCAATGCCAG GCTGACGACA GATGGGATCC CCCTCTTGCT AAGTGTACCT CTCGAACCCA CGACGCCCTG ATAGTGGGAA CCCTGTCCGG AACCATCTTC TTCATCCTGT TGATTATTTT TTTGAGCTGG ATCATCCTGA AACATAGGAA GGGAAATAAC GCCCACGAGA ACCCAAAGGA AGTCGCTATT CATCTGCATT CTCAGGGAGG ATCTTCAGTA CATCCTAGAA CACTGCAAAC CAATGAAGAA AATAGCAGGG TGCTCCCAGG ATCCGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGACT ACAAGGATGA CGATGACAAG TAGCGGCCGC GAAGGATCTG CGATCGCTCC GGTGCCCGTC AGTGGGCAGA GCGCACATCG CCCACAGTCC CCGAGAAGTT GGGGGGAGGG GTCGGCAATT GAACGGGTGC CTAGAGAAGG TGGCGCGGGG TAAACTGGGA AAGTGATGTC GTGTACTGGC TCCGCCTTTT TCCCGAGGGT GGGGGAGAAC CGTATATAAG TGCAGTAGTC GCCGTGAACG TTCTTTTTCG CAACGGGTTT GCCGCCAGAA CACAGCTGAA GCTTCGAGGG GCTCGCATCT CTCCTTCACG CGCCCGCCGC CCTACCTGAG GCCGCCATCC ACGCCGGTTG AGTCGCGTTC TGCCGCCTCC CGCCTGTGGT GCCTCCTGAA CTGCGTCCGC CGTCTAGGTA AGTTTAAAGC TCAGGTCGAG ACCGGGCCTT TGTCCGGCGC TCCCTTGGAG CCTACCTAGA CTCAGCCGGC TCTCCACGCT TTGCCTGACC CTGCTTGCTC AACTCTACGT CTTTGTTTCG TTTTCTGTTC TGCGCCGTTA CAGATCCAAG CTGTGACCGG CGCCTACGCT AGATGACCGA GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCCGGGCCG TACGCACCCT CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGACCCGG ACCGCCACAT CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA GGTGCCCGAA GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GAGTCGACAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAATCATCG TCCTTTCCTT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGGCCGCC TCCCCGCCTG GTACCTTTAA GACCAATGAC TTACAAGGCA GCTGTAGATC TTAGCCACTT TTTAAAAGAA AAGGGGGGAC TGGAAGGGCT AATTCACTCC CAACGAAAAT AAGATCTGCT TTTTGCTTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTAGTA GTTCATGTCA TCTTATTATT CAGTATTTAT AACTTGCAAA GAAATGAATA TCAGAGAGTG AGAGGAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGCTC TAGCTATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGACTTTT GCAGAGACGG CCCAAATTCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGCCAA GCTG
