BIFC-P53-USP7(human)
$99.00
In stock
SKU
PL21560
NM_003470.3;C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;TEF1
100μg liquid plasmid(Instantly):$120
| name | BIFC-P53-USP7(human) | another name | aBIFC-P53-USP7(human) |
| Insert Size (bp) | 3306bp | Plasmid host | Mammary cell |
| Plasmid uses | Bimolecular fluorescence | Fragment type | BIFC |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | SFFV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | BIFC-P53-MDM2 | 5′ sequencing primer | |
| 3′ sequencing primer | WPRE-R | Copy number | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC GCGCAATTAA CCCTCACTAA AGGGAACAAA AGCTGGAGCT GCAAGCTTAA TGTAGTCTTA TGCAATACTC TTGTAGTCTT GCAACATGGT AACGATGAGT TAGCAACATG CCTTACAAGG AGAGAAAAAG CACCGTGCAT GCCGATTGGT GGAAGTAAGG TGGTACGATC GTGCCTTATT AGGAAGGCAA CAGACGGGTC TGACATGGAT TGGACGAACC ACTGAATTGC CGCATTGCAG AGATATTGTA TTTAAGTGCC TAGCTCGATA CATAAACGGG TCTCTCTGGT TAGACCAGAT CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC CCTCAGACCC TTTTAGTCAG TGTGGAAAAT CTCTAGCAGT GGCGCCCGAA CAGGGACTTG AAAGCGAAAG GGAAACCAGA GGAGCTCTCT CGACGCAGGA CTCGGCTTGC TGAAGCGCGC ACGGCAAGAG GCGAGGGGCG GCGACTGGTG AGTACGCCAA AAATTTTGAC TAGCGGAGGC TAGAAGGAGA GAGATGGGTG CGAGAGCGTC AGTATTAAGC GGGGGAGAAT TAGATCGCGA TGGGAAAAAA TTCGGTTAAG GCCAGGGGGA AAGAAAAAAT ATAAATTAAA ACATATAGTA TGGGCAAGCA GGGAGCTAGA ACGATTCGCA GTTAATCCTG GCCTGTTAGA AACATCAGAA GGCTGTAGAC AAATACTGGG ACAGCTACAA CCATCCCTTC AGACAGGATC AGAAGAACTT AGATCATTAT ATAATACAGT AGCAACCCTC TATTGTGTGC ATCAAAGGAT AGAGATAAAA GACACCAAGG AAGCTTTAGA CAAGATAGAG GAAGAGCAAA ACAAAAGTAA GACCACCGCA CAGCAAGCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCT ATTCCAGCAC ATATGAGCTA GCTGCAGTAA CGCCATTTTG CAAGGCATGG AAAAATACCA AACCAAGAAT AGAGAAGTTC AGATCAAGGG CGGGTACATG AAAATAGCTA ACGTTGGGCC AAACAGGATA TCTGCGGTGA GCAGTTTCGG CCCCGGCCCG GGGCCAAGAA CAGATGGTCA CCGCAGTTTC GGCCCCGGCC CGAGGCCAAG AACAGATGGT CCCCAGATAT GGCCCAACCC TCAGCAGTTT CTTAAGACCC ATCAGATGTT TCCAGGCTCC CCCAAGGACC TGAAATGACC CTGCGCCTTA TTTGAATTAA CCAATCAGCC TGCTTCTCGC TTCTGTTCGC GCGCTTCTGC TTCCCGAGCT CTATAAAAGA GCTCACAACC CCTCACTCGG CGCGCCAGTC CTCCGACAGA CTGAGTCGGC CGGTCGAATC AAGCTTATCG ATAGCCACCA TGGAGGAGCC GCAGTCAGAT CCTAGCGTCG AGCCCCCTCT GAGTCAGGAA ACATTTTCAG ACCTATGGAA ACTACTTCCT GAAAACAACG TTCTGTCCCC CTTGCCGTCC CAAGCAATGG ATGATTTGAT GCTGTCCCCG GACGATATTG AACAATGGTT CACTGAAGAC CCAGGTCCAG ATGAAGCTCC CAGAATGCCA GAGGCTGCTC CCCCCGTGGC CCCTGCACCA GCAGCTCCTA CACCGGCGGC CCCTGCACCA GCCCCCTCCT GGCCCCTGTC ATCTTCTGTC CCTTCCCAGA AAACCTACCA GGGCAGCTAC GGTTTCCGTC TGGGCTTCTT GCATTCTGGG ACAGCCAAGT CTGTGACTTG CACGTACTCC CCTGCCCTCA ACAAGATGTT TTGCCAACTG GCCAAGACCT GCCCTGTGCA GCTGTGGGTT GATTCCACAC CCCCGCCCGG CACCCGCGTC CGCGCCATGG CCATCTACAA GCAGTCACAG CACATGACGG AGGTTGTGAG GCGCTGCCCC CACCATGAGC GCTGCTCAGA TAGCGATGGT CTGGCCCCTC CTCAGCATCT TATCCGAGTG GAAGGAAATT TGCGTGTGGA GTATTTGGAT GACAGAAACA CTTTTCGACA TAGTGTGGTG GTGCCCTATG AGCCGCCTGA GGTTGGCTCT GACTGTACCA CCATCCACTA CAACTACATG TGTAACAGTT CCTGCATGGG CGGCATGAAC CGGAGGCCCA TCCTCACCAT CATCACACTG GAAGACTCCA GTGGTAATCT ACTGGGACGG AACAGCTTTG AGGTGCGTGT TTGTGCCTGT CCTGGGAGAG ACCGGCGCAC AGAGGAAGAG AATCTCCGCA AGAAAGGGGA GCCTCACCAC GAGCTGCCCC CAGGGAGCAC TAAGCGAGCA CTGCCCAACA ACACCAGCTC CTCTCCCCAG CCAAAGAAGA AACCACTGGA TGGAGAATAT TTCACCCTTC AGATCCGTGG GCGTGAGCGC TTCGAGATGT TCCGAGAGCT GAATGAGGCC TTGGAACTCA AGGATGCCCA GGCTGGGAAG GAGCCAGGGG GGAGCAGGGC TCACTCCAGC CACCTGAAGT CCAAAAAGGG TCAGTCTACC TCCCGCCATA AAAAACTCAT GTTCAAGACA GAAGGGCCTG ACTCAGACAC CGGTGCTGAA GCTGCTGCAA AGGAAGCTGC AGCTAAGGAG GCTGCAGCTA AGGCTATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGCTGATCT GCACCACCGG CAAGCTGCCC GTGCCTTGGC CCACCCTCGT GACCACCCTG GGCTACGGCC TGCAGTGCTT CGCCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC TACAACAGCC ACAACGTCTA TCTCACCGCC TAAATGCATG AGTAACTGAG GATCCGCCCC TCTCCCTCCC CCCCCCCTAA CGTTACTGGC CGAAGCCGCT TGGAATAAGG CCGGTGTGCG TTTGTCTATA TGTTATTTTC CACCATATTG CCGTCTTTTG GCAATGTGAG GGCCCGGAAA CCTGGCCCTG TCTTCTTGAC GAGCATTCCT AGGGGTCTTT CCCCTCTCGC CAAAGGAATG CAAGGTCTGT TGAATGTCGT GAAGGAAGCA GTTCCTCTGG AAGCTTCTTG AAGACAAACA ACGTCTGTAG CGACCCTTTG CAGGCAGCGG AACCCCCCAC CTGGCGACAG GTGCCTCTGC GGCCAAAAGC CACGTGTATA AGATACACCT GCAAAGGCGG CACAACCCCA GTGCCACGTT GTGAGTTGGA TAGTTGTGGA AAGAGTCAAA TGGCTCTCCT CAAGCGTATT CAACAAGGGG CTGAAGGATG CCCAGAAGGT ACCCCATTGT ATGGGATCTG ATCTGGGGCC TCGGTACACA TGCTTTACAT GTGTTTAGTC GAGGTTAAAA AAACGTCTAG GCCCCCCGAA CCACGGGGAC GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCCTGGAAT TCGCCACCAT GAACCACCAG CAGCAGCAGC AGCAGCAGAA AGCGGGCGAG CAGCAGTTGA GCGAGCCCGA GGACATGGAG ATGGAAGCGG GAGATACAGA TGACCCACCA AGAATTACTC AGAACCCTGT GATCAATGGG AATGTGGCCC TGAGTGATGG ACACAACACC GCGGAGGAGG ACATGGAGGA TGACACCAGT TGGCGCTCCG AGGCAACCTT TCAGTTCACT GTGGAGCGCT TCAGCAGACT GAGTGAGTCG GTCCTTAGCC CTCCGTGTTT TGTGCGAAAT CTGCCATGGA AGATTATGGT GATGCCACGC TTTTATCCAG ACAGACCACA CCAAAAAAGC GTAGGATTCT TTCTCCAGTG CAATGCTGAA TCTGATTCCA CGTCATGGTC TTGCCATGCA CAAGCAGTGC TGAAGATAAT AAATTACAGA GATGATGAAA AGTCGTTCAG TCGTCGTATT AGTCATTTGT TCTTCCATAA AGAAAATGAT TGGGGATTTT CCAATTTTAT GGCCTGGAGT GAAGTGACCG ATCCTGAGAA AGGATTTATA GATGATGACA AAGTTACCTT TGAAGTCTTT GTACAGGCGG ATGCTCCCCA TGGAGTTGCG TGGGATTCAA AGAAGCACAC AGGCTACGTC GGCTTAAAGA ATCAGGGAGC GACTTGTTAC ATGAACAGCC TGCTACAGAC GTTATTTTTC ACGAATCAGC TACGAAAGGC TGTGTACATG ATGCCAACCG AGGGGGATGA TTCGTCTAAA AGCGTCCCTT TAGCATTACA AAGAGTGTTC TATGAATTAC AGCATAGTGA TAAACCTGTA GGAACAAAAA AGTTAACAAA GTCATTTGGG TGGGAAACTT TAGATAGCTT CATGCAACAT GATGTTCAGG AGCTTTGTCG AGTGTTGCTC GATAATGTGG AAAATAAGAT GAAAGGCACC TGTGTAGAGG GCACCATACC CAAATTATTC CGCGGCAAAA TGGTGTCCTA TATCCAGTGT AAAGAAGTAG ACTATCGGTC TGATAGAAGA GAAGATTATT ATGATATCCA GCTAAGTATC AAAGGAAAGA AAAATATATT TGAATCATTT GTGGATTATG TGGCAGTAGA ACAGCTCGAT GGGGACAATA AATACGACGC TGGGGAACAT GGCTTACAGG AAGCAGAGAA AGGTGTGAAA TTCCTAACAT TGCCACCAGT GTTACATCTA CAACTGATGA GATTTATGTA TGACCCTCAG ACGGACCAAA ATATCAAGAT CAATGATAGG TTTGAATTCC CAGAGCAGTT ACCACTTGAT GAATTTTTGC AAAAAACAGA TCCTAAGGAC CCTGCAAATT ATATTCTTCA TGCAGTCCTG GTTCATAGTG GAGATAATCA TGGTGGACAT TATGTGGTTT ATCTAAACCC CAAAGGGGAT GGCAAATGGT GTAAATTTGA TGACGACGTG GTGTCAAGGT GTACTAAAGA GGAAGCAATT GAGCACAATT ATGGGGGTCA CGATGACGAC CTGTCTGTTC GACACTGCAC TAATGCTTAC ATGTTAGTCT ACATCAGGGA ATCAAAACTG AGTGAAGTTT TACAGGCGGT CACCGACCAT GATATTCCTC AGCAGTTGGT GGAGCGATTA CAAGAAGAGA AAAGGATCGA GGCTCAGAAG CGGAAGGAGC GGCAGGAAGC CCATCTCTAT ATGCAAGTGC AGATAGTCGC AGAGGACCAG TTTTGTGGCC ACCAAGGGAA TGACATGTAC GATGAAGAAA AAGTGAAATA CACTGTGTTC AAAGTATTGA AGAACTCCTC GCTTGCTGAG TTTGTTCAGA GCCTCTCTCA GACCATGGGA TTTCCACAAG ATCAAATTCG ATTGTGGCCC ATGCAAGCAA GGAGTAATGG AACAAAACGA CCAGCAATGT TAGATAATGA AGCCGACGGC AATAAAACAA TGATTGAGCT CAGTGATAAT GAAAACCCTT GGACAATATT CCTGGAAACA GTTGATCCCG AGCTGGCTGC TAGTGGAGCG ACCTTACCCA AGTTTGATAA AGATCATGAT GTAATGTTAT TTTTGAAGAT GTATGATCCC AAAACGCGGA GCTTGAATTA CTGTGGGCAT ATCTACACAC CAATATCCTG TAAAATACGT GACTTGCTCC CAGTTATGTG TGACAGAGCA GGATTTATTC AAGATACTAG CCTTATCCTC TATGAGGAAG TTAAACCGAA TTTAACAGAG AGAATTCAGG ACTATGACGT GTCTCTTGAT AAAGCCCTTG ATGAACTAAT GGATGGTGAC ATCATAGTAT TTCAGAAGGA TGACCCTGAA AATGATAACA GTGAATTACC CACCGCAAAG GAGTATTTCC GAGATCTCTA CCACCGCGTT GATGTCATTT TCTGTGATAA AACAATCCCT AATGATCCTG GATTTGTGGT TACGTTATCA AATAGAATGA ATTATTTTCA GGTTGCAAAG ACAGTTGCAC AGAGGCTCAA CACAGATCCA ATGTTGCTGC AGTTTTTCAA GTCTCAAGGT TATAGGGATG GCCCAGGTAA TCCTCTTAGA CATAATTATG AAGGTACTTT AAGAGATCTT CTACAGTTCT TCAAGCCTAG ACAACCTAAG AAACTTTACT ATCAGCAGCT TAAGATGAAA ATCACAGACT TTGAGAACAG GCGAAGTTTT AAATGTATAT GGTTAAACAG CCAATTTAGG GAAGAGGAAA TAACACTATA TCCAGACAAG CATGGGTGTG TCCGGGACCT GTTAGAAGAA TGTAAAAAGG CCGTGGAGCT TGGGGAGAAA GCATCAGGGA AACTTAGGCT GCTAGAAATT GTAAGCTACA AAATCATTGG TGTTCATCAA GAAGATGAAC TATTAGAATG TTTATCTCCT GCAACGAGCC GGACGTTTCG AATAGAGGAA ATCCCTTTGG ACCAGGTGGA CATAGACAAA GAGAATGAGA TGCTTGTCAC AGTGGCGCAT TTCCACAAAG AGGTCTTCGG AACGTTCGGA ATCCCGTTTT TGCTGAGGAT ACACCAGGGC GAGCATTTTC GAGAAGTGAT GAAGCGAATC CAGAGCCTGC TGGACATCCA GGAGAAGGAG TTTGAGAAGT TTAAATTTGC AATTGTAATG ATGGGCCGAC ACCAGTACAT AAATGAAGAC GAGTATGAAG TAAATTTGAA AGACTTTGAG CCACAGCCCG GTAATATGTC TCATCCTCGG CCTTGGCTAG GGCTCGACCA CTTCAACAAA GCCCCAAAGA GGAGTCGCTA CACTTACCTT GAAAAGGCCA TTAAAATCCA TAACGGAGGC GGTAGTGGAG GCGGTAGTGG AGGCGGTAGT GACAAGCAGA AGAACGGCAT CAAGGCCAAC TTCAAGATCC GCCACAACAT CGAGGACGGC GGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCTACCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTAAACTAG TAAGCTTGGC GTAACTAGAT CTTGAGACAA ATGGCAGTAT TCATCCACAA TTTTAAAAGA AAAGGGGGGA TTGGGGGGTA CAGTGCAGGG GAAAGAATAG TAGACATAAT AGCAACAGAC ATACAAACTA AAGAATTACA AAAACAAATT ACAAAAATTC AAAATTTTCG GGTTTATTAC AGGGACAGCA GAGATCCACT TTGGGCTCGA GCTTGGTTCA TTCTCAAGCC TACGCGTTAA GTCGACAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AATCATCGTC CTTTCCTTGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCGTCGA CTTTAAGACC AATGACTTAC AAGGCAGCTG TAGATCTTAG CCACTTTTTA AAAGAAAAGG GGGGACTGGA AGGGCTAATT CACTCCCAAC GAAGACAAGA TCTGCTTTTT GCTTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GTACGTATAG TAGTTCATGT CATCTTATTA TTCAGTATTT ATAACTTGCA AAGAAATGAA TATCAGAGAG TGAGAGGAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGGC TCTAGCTATC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGGA CGTACCCAAT TCGCCCTATA GTGAGTCGTA TTACGCGCGC TCACTGGCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGCTTA CAATTTAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGC
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