NM_001199397.3;ALS24; NY-REN-55; OFD2; SRPS2; SRPS2A;SRTD6
100μg liquid plasmid(Instantly):$120
| name | pHAGE-3×FLAG-NEK1(human)-Puro | another name | apHAGE-3×FLAG-NEK1(human)-Puro |
| Insert Size (bp) | 3861bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | pHAGE-3×FLAG-MCS-Puro | 5′ sequencing primer | CMV-F |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGGAAGGGCT AATTCACTCC CAAAGAAGAC AAGATATCCT TGATCTGTGG ATCTACCACA CACAAGGCTA CTTCCCTGAT TAGCAGAACT ACACACCAGG GCCAGGGGTC AGATATCCAC TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCC AGATAAGGTA GAAGAGGCCA ATAAAGGAGA GAACACCAGC TTGTTACACC CTGTGAGCCT GCATGGGATG GATGACCCGG AGAGAGAAGT GTTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC GTGGCCCGAG AGCTGCATCC GGAGTACTTC AAGAACTGCT GATATCGAGC TTGCTACAAG GGACTTTCCG CTGGGGACTT TCCAGGGAGG CGTGGCCTGG GCGGGACTGG GGAGTGGCGA GCCCTCAGAT CCTGCATATA AGCAGCTGCT TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCTCGACGG TATCGCCGAA TTCACAAATG GCAGTATTCA TCCACAATTT TAAAAGAAAA GGGGGGATTG GGGGGTACAG TGCAGGGGAA AGAATAGTAG ACATAATAGC AACAGACATA CAAACTAAAG AATTACAAAA ACAAATTACA AAAATTCAAA ATTTTCGGGT TTATTACAGG GACAGCAGAG ATCCAGTTTG GACTAGTGGA GTTCCGCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGACAC CGGCGGCCAC GCGTGCCACC ATGGATTACA AGGATGACGA CGATAAGGAC TATAAGGACG ATGATGACAA GGACTACAAA GATGATGACG ATAAACGAAG CCCGGGCGGA TCGGGTTTAA ACGGATCCAT GGAGAAGTAT GTTAGACTAC AGAAGATTGG AGAAGGTTCA TTTGGAAAAG CCATTCTTGT TAAATCTACA GAAGATGGCA GACAGTATGT TATCAAGGAA ATTAACATCT CAAGAATGTC CAGTAAAGAA AGAGAAGAAT CAAGGAGAGA AGTTGCAGTA TTGGCAAACA TGAAGCATCC AAATATTGTC CAGTATAGAG AATCATTTGA AGAAAATGGC TCTCTCTACA TAGTAATGGA TTACTGTGAG GGAGGGGATC TGTTTAAGCG AATAAATGCT CAGAAAGGCG TTTTGTTTCA AGAGGATCAG ATTTTGGACT GGTTTGTACA GATATGTTTG GCCCTGAAAC ATGTACATGA TAGAAAAATT CTTCATCGAG ACATTAAATC TCAGAACATA TTTTTAACTA AAGATGGAAC AGTACAACTT GGAGATTTTG GAATTGCTAG AGTTCTTAAT AGTACTGTAG AGCTGGCTCG AACTTGCATA GGGACCCCAT ACTACTTGTC ACCTGAAATC TGTGAAAACA AACCTTACAA TAATAAAAGT GACATTTGGG CTCTGGGGTG TGTCCTTTAT GAGCTGTGTA CACTTAAACA TGCTTTTGAA GCTGGCAGTA TGAAAAACCT GGTACTGAAG ATAATATCTG GATCTTTTCC ACCTGTGTCT TTGCATTATT CCTATGATCT CCGCAGTTTG GTGTCTCAGT TATTTAAAAG AAATCCTAGG GATAGACCAT CAGTCAACTC CATATTGGAG AAAGGTTTTA TAGCCAAACG CATTGAAAAG TTTCTCTCTC CTCAGCTTAT TGCAGAAGAA TTTTGTCTAA AAACATTTTC GAAGTTTGGA TCACAGCCTA TACCAGCTAA AAGACCAGCT TCAGGACAAA ACTCGATTTC TGTTATGCCT GCTCAGAAAA TTACAAAGCC TGCCGCTAAA TATGGAATAC CTTTAGCATA TAAGAAATAT GGAGATAAAA AATTACACGA AAAGAAACCA CTGCAAAAAC ATAAACAGGC CCATCAAACT CCAGAGAAGA GAGTGAATAC TGGAGAAGAA AGGAGGAAAA TATCTGAGGA AGCAGCAAGA AAGAGAAGGC TGGAATTTAT TGAAAAAGAA AAGAAACAAA AGGATCAGAT TATTAGTTTA ATGAAGGCTG AACAAATGAA AAGGCAAGAA AAGGAAAGGT TGGAAAGAAT AAATAGGGCC AGGGAACAAG GATGGAGAAA TGTGCTAAGT GCTGGTGGAA GTGGTGAAGT AAAGGCTCCT TTTCTGGGCA GTGGAGGGAC TATAGCTCCA TCATCTTTTT CTTCTCGAGG ACAGTATGAA CATTACCATG CCATTTTTGA CCAAATGCAG CAACAAAGAG CAGAAGATAA TGAAGCTAAA TGGAAAAGAG AAATATATGG TCGAGGTCTT CCAGAAAGAG GAATTCTGCC TGGAGTTCGT CCAGGATTTC CTTATGGGGC TGCAGGTCAT CACCATTTTC CTGATGCTGA TGATATTAGA AAAACTTTGA AAAGATTGAA GGCGGTGTCT AAACAAGCCA ATGCAAACAG GCAAAAAGGG CAGCTAGCTG TAGAAAGAGC TAAACAAGTA GAAGAGTTCC TGCAGCGAAA ACGGGAAGCT ATGCAGAATA AAGCTCGAGC CGAAGGACAT ATGGGAATCC TGCAAAACCT GGCAGCTATG TATGGAGGCA GGCCCAGCTC TTCAAGAGGA GGGAAGCCAA GAAACAAAGA GGAAGAGGTT TATCTGGCAA GACTGAGGCA AATAAGACTA CAGAATTTCA ATGAGCGCCA ACAGATTAAA GCCAAACTTC GTGGTGAAAA GAAAGAAGCT AATCATTCTG AAGGACAAGA AGGAAGTGAA GAGGCTGACA TGAGGCGCAA AAAAATCGAA TCACTGAAGG CCCATGCAAA TGCACGTGCT GCTGTACTAA AAGAACAACT AGAACGAAAG AGAAAGGAGG CTTATGAGAG AGAAAAAAAA GTGTGGGAAG AGCATTTGGT GGCTAAAGGA GTTAAGAGTT CTGATGTTTC TCCACCTTTG GGACAGCATG AAACAGGTGG CTCTCCATCA AAGCAACAGA TGAGATCTGT TATTTCTGTA ACTTCAGCTT TGAAAGAAGT TGGCGTGGAC AGTAGTTTAA CTGATACCCG GGAAACTTCA GAAGAGATGC AAAAGACCAA CAATGCTATT TCAAGTAAGC GAGAAATACT TCGTAGATTA AATGAAAATC TTAAAGCTCA AGAAGATGAA AAAGGAAAGC AGAATCTCTC TGATACTTTT GAGATAAATG TTCATGAAGA TGCCAAAGAG CATGAAAAAG AAAAATCAGT TTCATCTGAT CGCAAGAAGT GGGAGGCAGG AGGTCAACTT GTGATTCCTC TGGATGAGTT AACACTAGAT ACATCCTTCT CTACAACTGA AAGACATACA GTGGGAGAAG TTATTAAATT AGGTCCTAAT GGATCTCCAA GAAGAGCCTG GGGGAAAAGT CCGACAGATT CTGTTCTAAA GATACTTGGA GAAGCTGAAC TACAACTTCA GACAGAACTA TTAGAAAATA CAACTATTAG AAGTGAGATT TCTCCCGAAG GGGAAAAGTA CAAACCCTTA ATTACTGGAG AAAAAAAAGT ACAATGTATT TCACATGAAA TAAACCCATC AGCTATTGTT GATTCTCCTG TTGAGACAAA AAGTCCCGAG TTCAGTGAGG CATCTCCACA GATGTCATTG AAACTGGAAG GAAATTTAGA AGAACCTGAT GATTTGGAAA CAGAAATTCT ACAAGAGCCA AGTGGAACAA ACAAAGATGA GAGCTTGCCA TGCACTATTA CTGATGTGTG GATTAGTGAG GAAAAAGAAA CAAAGGAAAC TCAGTCGGCA GATAGGATCA CCATTCAGGA AAATGAAGTT TCTGAAGATG GAGTCTCGAG TACTGTGGAC CAACTTAGTG ACATTCATAT AGAGCCTGGA ACCAATGATT CTCAGCACTC TAAATGTGAT GTAGATAAGT CTGTGCAACC GGAACCATTT TTCCATAAGG TGGTTCATTC TGAACACTTG AACTTAGTCC CTCAAGTTCA ATCAGTTCAG TGTTCACCAG AAGAATCCTT TGCATTTCGA TCTCACTCGC ATTTACCACC AAAAAATAAA AACAAGAATT CCTTGCTGAT TGGACTTTCA ACTGGTCTGT TTGATGCAAA CAACCCAAAG ATGTTAAGGA CATGTTCACT TCCAGATCTC TCAAAGCTGT TCAGAACCCT TATGGATGTT CCCACCGTAG GAGATGTTCG TCAAGACAAT CTTGAAATAG ATGAAATTGA AGATGAAAAC ATTAAAGAAG GACCTTCTGA TTCTGAAGAC ATTGTGTTTG AAGAAACTGA CACAGATTTA CAAGAGCTGC AGGCCTCGAT GGAACAGTTA CTTAGGGAAC AACCTGGTGA AGAATACAGT GAAGAAGAAG AGTCAGTCTT GAAGAACAGT GATGTGGAGC CAACTGCAAA TGGGACAGAT GTGGCAGATG AAGATGACAA TCCCAGCAGT GAAAGTGCCC TGAACGAAGA ATGGCACTCA GATAACAGTG ATGGTGAAAT TGCTAGTGAA TGTGAATGCG ATAGTGTCTT TAACCATTTA GAGGAACTGA GACTTCATCT GGAGCAGGAA ATGGGCTTTG AAAAATTCTT TGAGGTTTAT GAGAAAATAA AGGCTATTCA TGAAGATGAA GATGAAAATA TTGAAATTTG TTCAAAAATA GTTCAAAATA TTTTGGGAAA TGAACATCAG CATCTTTATG CCAAGATTCT TCATTTAGTC ATGGCAGATG GAGCCTACCA AGAAGATAAT GATGAATAAC TCGAGTCTAG AGGGCCCTTA GTCGAGAATT CTACCGGGTA GGGGAGGCGC TTTTCCCAAG GCAGTCTGGA GCATGCGCTT TAGCAGCCCC GCTGGGCACT TGGCGCTACA CAAGTGGCCT CTGGCCTCGC ACACATTCCA CATCCACCGG TAGGCGCCAA CCGGCTCCGT TCTTTGGTGG CCCCTTCGCG CCACCTTCTA CTCCTCCCCT AGTCAGGAAG TTCCCCCCCG CCCCGCAGCT CGCGTCGTGC AGGACGTGAC AAATGGAAGT AGCACGTCTC ACTAGTCTCG TGCAGATGGA CAGCACCGCT GAGCAATGGA AGCGGGTAGG CCTTTGGGGC AGCGGCCAAT AGCAGCTTTG CTCCTTCGCT TTCTGGGCTC AGAGGCTGGG AAGGGGTGGG TCCGGGGGCG GGCTCAGGGG CGGGCTCAGG GGCGGGGCGG GCGCCCGAAG GTCCTCCGGA GGCCCGGCAT TCTGCACGCT TCAAAAGCGC ACGTCTGCCG CGCTGTTCTC CTCTTCCTCA TCTCCGGGCC TTTCGACCTG CAGCCCAAGC TTACCATGAC CGAGTACAAG CCCACGGTGC GCCTCGCCAC CCGCGACGAC GTCCCCAGGG CCGTACGCAC CCTCGCCGCC GCGTTCGCCG ACTACCCCGC CACGCGCCAC ACCGTCGATC CGGACCGCCA CATCGAGCGG GTCACCGAGC TGCAAGAACT CTTCCTCACG CGCGTCGGGC TCGACATCGG CAAGGTGTGG GTCGCGGACG ACGGCGCCGC GGTGGCGGTC TGGACCACGC CGGAGAGCGT CGAAGCGGGG GCGGTGTTCG CCGAGATCGG CCCGCGCATG GCCGAGTTGA GCGGTTCCCG GCTGGCCGCG CAGCAACAGA TGGAAGGCCT CCTGGCGCCG CACCGGCCCA AGGAGCCCGC GTGGTTCCTG GCCACCGTCG GCGTCTCGCC CGACCACCAG GGCAAGGGTC TGGGCAGCGC CGTCGTGCTC CCCGGAGTGG AGGCGGCCGA GCGCGCCGGG GTGCCCGCCT TCCTGGAGAC CTCCGCGCCC CGCAACCTCC CCTTCTACGA GCGGCTCGGC TTCACCGTCA CCGCCGACGT CGAGGTGCCC GAAGGACCGC GCACCTGGTG CATGACCCGC AAGCCCGGTG CCTGAATCGA TAGATCCTAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAATCATCG TCCTTTCCTT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGGCCGCC TCCCCGCCTG AGATCCTTTA AGACCAATGA CTTACAAGGC AGCTGTAGAT CTTAGCCACT TTTTAAAAGA AAAGGGGGGA CTGGAAGGGC TAATTCACTC CCAACGAAGA CAAGATCTGC TTTTTGCTTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGTAGT AGTTCATGTC ATCTTATTAT TCAGTATTTA TAACTTGCAA AGAAATGAAT ATCAGAGAGT GAGAGGCCCG GGTTAATTAA GGAAAGGGCT AGATCATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CAAGCTCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCCGTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACA TGATTACGAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGGA TCAACTGGAT AACTCAAGCT AACCAAAATC ATCCCAAACT TCCCACCCCA TACCCTATTA CCACTGCCAA TTACCTGTGG TTTCATTTAC TCTAAACCTG TGATTCCTCT GAATTATTTT CATTTTAAAG AAATTGTATT TGTTAAATAT GTACTACAAA CTTAGTAGT
