pLV3-CMV-Ldlr(mouse)-EGFP-EF1a-Puro
$106.00
In stock
SKU
PL21157
100μg liquid plasmid(Instantly):$105
| name | pLV3-CMV-Ldlr(mouse)-EGFP-EF1a-Puro | another name | apLV3-CMV-Ldlr(mouse)-EGFP-EF1a-Puro |
| Insert Size (bp) | Plasmid host | ||
| Plasmid uses | Fragment type | ||
| Fragment species | Prokaryotic resistance | ||
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | ||
| Competence | Growth Temperature | ||
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTTAACTTT TAAAAGAAAA GGGGGGATTG GGGGGTACAG TGCAGGGGAA AGAATAGTAG ACATAATAGC AACAGACATA CAAACTAAAG AATTACAAAA ACAAATTACA AAAATTCAAA ATTTTATCGA TACTAGTATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCG TTTAGTGAAC CGTCAGATCG CCTGGAGACG CCATCCACGC TGTTTTGACC TCCATAGAAG ATTCTAGAGC TAGCGAATTC GCCACCATGA GCACCGCGGA TCTGATGCGT CGCTGGGTCA TCGCCCTGCT CCTGGCTGCT GCCGGAGTTG CAGCAGAAGA CTCATGCAGC AGGAACGAGT TCCAGTGTAG AGACGGAAAA TGCATCGCTA GCAAGTGGGT GTGCGATGGC AGCCCCGAGT GCCCGGATGG CTCCGATGAG TCCCCAGAGA CATGCATGTC TGTCACCTGT CAGTCCAATC AATTCAGCTG TGGAGGCCGT GTCAGCCGAT GCATTCCTGA CTCCTGGAGA TGTGATGGAC AGGTAGACTG TGAAAATGAC TCAGACGAAC AAGGCTGTCC CCCCAAGACG TGCTCCCAGG ATGACTTCCG ATGCCAGGAT GGCAAGTGCA TCTCCCCGCA GTTTGTGTGT GATGGAGACC GAGATTGCCT AGATGGCTCT GATGAGGCCC ACTGCCAGGC CACCACTTGT GGCCCCGCCC ACTTCCGCTG CAACTCATCC ATATGCATCC CCAGTCTTTG GGCCTGCGAC GGGGATGTCG ACTGTGTTGA CGGCTCCGAT GAGTGGCCAC AGAACTGCCA GGGCCGAGAC ACGGCCTCCA AAGGCGTTAG CAGCCCCTGC TCCTCCCTGG AGTTCCACTG TGGTAGCAGT GAGTGTATCC ATCGCAGCTG GGTCTGTGAC GGCGAGGCAG ACTGCAAGGA CAAGTCAGAT GAGGAGCACT GCGCGGTGGC CACCTGCCGA CCTGATGAAT TCCAGTGTGC AGATGGCTCC TGCATTCACG GTAGCCGCCA GTGTGACCGT GAACATGACT GCAAGGACAT GAGCGACGAG CTCGGCTGCG TCAATGTGAC ACAGTGTGAT GGCCCCAACA AGTTCAAGTG TCACAGTGGG GAGTGCATCA GCTTGGACAA GGTGTGCGAC TCCGCCCGCG ACTGCCAGGA CTGGTCGGAT GAGCCCATCA AGGAGTGCAA GACCAACGAG TGTTTGGACA ACAATGGTGG CTGTTCCCAC ATCTGCAAGG ACCTCAAGAT TGGCTCTGAG TGCCTGTGTC CCAGCGGCTT CCGGTTGGTG GACCTCCACA GGTGTGAAGA TATTGACGAG TGTCAGGAGC CAGACACCTG CAGCCAGCTC TGTGTGAACC TGGAAGGCAG CTACAAGTGT GAGTGCCAGG CCGGCTTCCA CATGGACCCA CACACCAGGG TCTGCAAGGC TGTGGGCTCC ATAGGCTATC TGCTCTTCAC CAACCGCCAC GAGGTCCGGA AGATGACCCT GGACCGCAGC GAGTACACCA GTCTGCTCCC CAACCTGAAG AATGTGGTGG CTCTCGACAC GGAGGTGACC AACAATAGAA TCTACTGGTC CGACCTGTCC CAAAAAAAGA TCTACAGCGC CCTGATGGAC CAGGCCCCTA ACTTGTCCTA CGACACCATC ATCAGTGAGG ACCTGCATGC CCCTGACGGG CTGGCGGTAG ACTGGATCCA CCGCAACATC TACTGGACAG ATTCAGTCCC AGGCAGCGTA TCTGTGGCTG ACACCAAGGG CGTAAAGAGG AGGACACTGT TCCAAGAGGC AGGGTCCAGA CCCAGAGCCA TCGTAGTGGA CCCTGTGCAT GGCTTCATGT ACTGGACAGA TTGGGGAACA CCCGCCAAGA TCAAGAAAGG GGGTTTGAAT GGTGTGGACA TCCACTCACT GGTGACCGAA AACATCCAGT GGCCAAATGG CATCACACTA GATCTTTCCA GTGGCCGTCT CTATTGGGTT GATTCCAAAC TCCACTCTAT CTCCAGCATC GATGTCAATG GGGGCAATCG GAAAACCATT TTGGAGGATG AGAACCGGCT GGCCCACCCC TTCTCCTTGG CCATCTATGA GGACAAAGTG TATTGGACAG ATGTCATAAA CGAAGCCATT TTCAGTGCCA ATCGACTCAC GGGTTCAGAT GTGAATTTGG TGGCTGAAAA CCTCTTGTCC CCGGAGGACA TTGTCCTGTT CCACAAGGTC ACACAGCCTA GAGGGGTGAA CTGGTGTGAG ACAACAGCCC TCCTCCCCAA TGGTGGTTGC CAGTACCTGT GCCTGCCCGC CCCACAGATC GGTCCCCACT CGCCCAAATT CACCTGCGCC TGCCCTGATG GCATGCTGCT GGCCAAGGAC ATGCGGAGCT GCCTCACAGA AGTCGACACT GTACTGACCA CCCAGGGGAC ATCCGCCGTC CGGCCTGTGG TCACCGCATC AGCTACCAGG CCACCGAAGC ACAGTGAGGA TCTCTCAGCT CCCAGTACTC CTAGGCAGCC TGTGGACACC CCAGGGCTCA GCACAGTGGC GTCAGTGACA GTGTCCCACC AAGTCCAGGG TGACATGGCT GGCAGAGGGA ATGAGGAGCA GCCACATGGT ATGAGGTTCC TGTCCATCTT CTTCCCTATT GCACTGGTTG CCCTCCTTGT CCTTGGGGCC GTCCTGCTGT GGAGGAACTG GCGGCTGAAG AACATCAACA GCATAAACTT TGACAACCCA GTCTACCAGA AGACCACAGA GGACGAGCTC CACATTTGCC GAAGCCAGGA TGGCTATACC TACCCCTCAA GACAGATGGT CAGCCTGGAG GACGATGTGG CAGGATCCGG AGGAGGGGGA AGCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG TAAGCGGCCG CGAAGGATCT GCGATCGCTC CGGTGCCCGT CAGTGGGCAG AGCGCACATC GCCCACAGTC CCCGAGAAGT TGGGGGGAGG GGTCGGCAAT TGAACGGGTG CCTAGAGAAG GTGGCGCGGG GTAAACTGGG AAAGTGATGT CGTGTACTGG CTCCGCCTTT TTCCCGAGGG TGGGGGAGAA CCGTATATAA GTGCAGTAGT CGCCGTGAAC GTTCTTTTTC GCAACGGGTT TGCCGCCAGA ACACAGCTGA AGCTTCGAGG GGCTCGCATC TCTCCTTCAC GCGCCCGCCG CCCTACCTGA GGCCGCCATC CACGCCGGTT GAGTCGCGTT CTGCCGCCTC CCGCCTGTGG TGCCTCCTGA ACTGCGTCCG CCGTCTAGGT AAGTTTAAAG
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