pCMV-HA-Cep164(mouse)-mCherry-Neo
$99.00
In stock
SKU
PL20962
NM_001081373.3;D030051D21
100μg liquid plasmid(Instantly):$120
| name | pCMV-HA-Cep164(mouse)-mCherry-Neo | another name | apCMV-HA-Cep164(mouse)-mCherry-Neo |
| Insert Size (bp) | 3999bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | Red |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pmCherry-N1 | 5′ sequencing primer | CMV-F |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGACTCAG ATCTCGAGCT CAAGCTTCGA ATTCGCCACC ATGTACCCAT ACGACGTCCC AGACTACGCT ATGGCTAGAC GGCCCATCCT TTTAGGTGAC CAGCTGGTCC TGGAGGAAGA TTCTGATGAG ACCTACGTTC CCAGTGAGCA AGAAATCCTT GACTTTGCCA GAGTGATTGG TATTGACCCT ATCAAGGAAC CAGAACTGAT GTGGCTGGCG AGAGAGGGTA TTGAGGCTCC ATTGCCCAAG GGCTGGAAAC CATGCCAGAA CATCACAGGT GATCTTTACT ATTTCAACTT TGACACGGGG CAGTCCATCT GGGACCATCC ATGTGACGAG CACTATCGAA AGTTGGTCAT CCAGGAGAGG GAGAGGTGGT CAGCTCCCGG GGCCATTAAG AAGAAAGACA AGAAAAAGAA AAAGGAAAAG AAAAACAAGA AGGACAAAGA GACCTCCAAA AGTCCTCTGG TCCTGGGTTC CCCCTTAGCT CTAGTCCAGG CCCCTCTTTG GGGCCTGGCT CCCTTACGAG GCCTTGGGGA TGCCCCACCC TCTGCTCTTC GTGGGTCGCA GAGCGTGAGC TTGGGCAGCT CAGCGGATTC TGGGCATCTT GGGGAGCCCA CGCTGCCTCC ACAGGGTCTC AAGGCTGCTG CTTGTGCAAA GGGTCTCTTG GCTTCTGTCC ATGAGGGCAA GAATGCTCTC AGCCTCTTGA CTTTAGGGGA AGAGACCAAC GAGGAGGATG AAGAGGAGAG TGACAACCAG AGTGTCCGGA GCTCCAGTGA ACTTCTCAAG AACCTACATC TGGACCTTGG TGCCCTGGGA GGTAACTTTG AGTACGAGGA GTCTCCAAGA ACCAGCCAGC CAGACAAGAA AGATGTTTCT CTGGACTCAG ATGCCGATCG CCCCCCAACT CCCGGCAAGC TGTTCAGCCA GGGTGCAGAC AGTAGTGTGG CCAGTGCCAA TGGCAGCAAG TCCCAAGGAA GAGGGGCAAG TCCCTGGAAC CCACAGAAAG AAAACGAGAA TAGTGATCCC AAGGCTTCTT CAAGTCAGAT GGCTCCCGAG TTGGACCCCG GGGGTGATCA GCCTTCCAGG GCCTCTAAAA AGCAGCAGGC AGAGGACCCA GTGCAGGCAG GAAAGGAGGG AGAGTGCAGG AGGGAGAGTG CAGCCAAGGA GCCAAAGGAG GCTTCTGCCT TGGAGAACAC CTCAGATGTC AGTGAGGAGT CAGAGATCCA CGGACACTTG AAGGACGCCC GGCACTCAGG GTCCGAAGCT TCTGGCCCTA AGTCTTTCCT GGGCCTGGAC TTGGGCTTTC GCAGCCGCAT CTCGGAGCAT CTGCTGGATG GTGACACACT TTCTCCAGTC TTGGGTGGAG GCCACTGGGA GGCCCAGGGG TTAGACCAAG AAGAACAAGA TGACAGCAAA TCCAGCATCG CTGAGCCACA GAGCAAGCAT ACCCAAGGCT CCGAGAGGGA ACATCTCCAG AGTAGCCTTC ACAGCCAGGC TACTGAGGAA GGGCCTTTGC AGACCCTGGA GGGGCAGCCT GAGTGGAAGG AAGCAGAGGG ACCTGGGAAG GACTCTGTAG CCAGCCCCGC CCCACTGTCC CTCCTCCAGA GCCTCCTGAA AGCACAGCTA CAGAAAGCCA CCGCTGAGGA GAAGGAGAAG GAAGAGGAGA CTAAGATAAG AGAGGAGGAA AGCCGGAGGC TGGTCTGCCT CCGGGCCCAG GTGCAGTCCA GGACTGAGGC TTTTGAGAAC CAAATTAGAA CCGAGCAACA GGCTGCCCTA CAGAGGCTTC GAGAAGAGGC CGAGACTCTC CAGAAAGCTG AAAGAGCCAG CTTGGAACAG AAAAGCAGGA GGGCACTGGA GCAGCTGAGG GAGCAGCTGG AGGCCGAGGA GAGGAGTGCC CAGGCTGCCT TGAGGGCAGA GAAGGAGGCG GAGAAGGAGG CCGCTCTGCT GCAGCTGAGG GAGCAGCTGG AAGGGGAGAG GAAGGAAGCA GTGGCAGGCC TAGAGAAGAA GCACAGCGCT GAGCTGGAAC AGCTCTGTTC CTCACTGGAA GCCAAACACC AGGAGGTGAT CTCCAGCCTC CAGAAGAAGA TAGAGGGAGC TCAGCAGAAA GAGGAGGCCC AATTACAGGA GAGCCTTGGG TGGGCCGAGC AGAGAGCTCA CCAGAAGGTT CACCAGGTGA CCGAGTATGA ACAGGAGCTC AGCAGCCTCC TTCGAGACAA GCGCCAGGAG GTAGAGAGGG AACACGAGCG GAAGATGGAC AAGATGAAGG AGGAGCACTG GCAAGAGATG GCTGACGCCA GAGAGCGATA CGAAGCCGAG GAGAGAAAGC AGCGGGCTGA CCTCCTGGGG CACCTGACTG GAGAGCTGGA GCGCCTGCGA AGGGCCCACG AGCGGGAGTT AGAGAGTATG AGGCAGGAGC AGGACCAGCA GCTTGAAGAC CTGAGGCGTC GACACCGCGA CCATGAAAGG AAATTACAAG ACTTAGAGGT GGAACTCTCG TCCAGAACTA AAGATGTCAA GGCCAGACTA GCTCAGCTGA ATGTTCAGGA GGAGAACATA CGGAAAGAGA AGCAGCTGCT CCTGGATGCG CAGAGGCAGG CTGCTCTGGA GAGGGAGGAA GCCACAGCCA CCCACCAGCA CCTGGAAGAA GCCAAGAAAG AGCACACACA CCTGCTGGAG ACGAAGCAGC AGCTGCGAAG GACCATCGAT GACCTGAGGG TCCGGCGGGT GGAGCTGGAG TCCCAAGTGG ACCTGTTGCA GGCGCAGAGT CAGAGATTGC AGAAGCACCT GAGCAGCCTA GAGGCCGAAG TACAAAGGAA GCAGGATGTC TTGAAAGAGA TGGCAGCTGA GATGAATGCT TCCCCACACC CGGAGCCAGG GCTCCACATT GAGGACCTGA GGAAATCCCT TGACACAAAT AAGAACCAAG AGGTGTCCTC TTCTCTCTCC CTGAGTAAGG AGGAGATCGA CCTGTCTATG GAGAGTGTCC GACAGTTCCT CTCTGCTGAG GGTGTAGCTG TGCGCAACGC TAAGGAGTTC CTGGTGCGCC AGACACGCTC CATGCGGAGG CGACAGACAG CTCTGAAAGC CGCCCAGCAG CACTGGCGCC ATGAGCTGGC CAGTGCCCAG GAGGTAGACG AAGACCTGCC AGGCACCGAG GTCCTGGGAA ACATGCGCAA GAACTTGAAT GAGGAGACCA GGCACCTGGA TGAGATGAAG TCAGCCATGC GCAAAGGCCA TGACTTGTTG AAGAAGAAAG AGGAGAAGCT GATCCAACTG GAATCTTCTC TTCAGGAGGA GGTCTCTGAC GAGGACACTC TGAAAGGATC CTCTATCAAG AAGGTGACCT TTGATCTCAG TGACATGGAC GATTTGAGCA GTGAGAGTCT TGAGTCCAGC CCCGTGCTGC ACATCACCCC GACCCCGACC TCTGCTGATC CCAACAAGAT CCATTACCTG AGCAGCTCCC TTCAGCGAAT CAGCAGTGAG CTAAACGGCG TGCTGAATGT GCTGGGAAGC CTCAACTCCC AGCCGCCGCC CCAGGGCCTC GGCAGCCAGC CCCCGCCTCC ACTCTTCACC TCCTCACTTC GGTCCTCCAA GAATGTCCTT GACCCTGCCT ACTCCTCCCA GGCCAAGCTC TCCTCCTTAT CTTCCATCAC ACCCATGTCC ACCCAGTGGG CCTGGGACCC AGGGCAGGGC ACCAAGCTGA CCTCTTCCTC CTCCTCTCAA ACTGTGGATG ATTTCCTGCT GGAGAAGTGG CGCAAGTATT TTCCCTCTGG CATCCCGTTG CTCAGTGGCT CTCCTCCCCC ACCGGAGAAC AAGCTGGGTT ATGTGTCTGT AAGTGAGCAG CTCCACTTCC TGCAGCGCTC CCACCCTCGA GTCCCCAGGA CGGATGGTGT CAGCATTCAG AGCTTGATCG ATTCCAACCG GAAGTGGCTG GAACATTTCC GGAACGACCC CAAGGTACAG CTTTTCTCCT CGGCGCCCAA AGCAACAACC ACATCGAACT TATCGAACCT CCTGCAGCTG GGCCTGGACG AGAACAACAG ACTGAATGTG TTTCATTACG GATCCGGCGG CGGAGGGAGT GGAGGAGGGG GAAGCATGGT GAGCAAGGGC GAGGAGGATA ACATGGCCAT CATCAAGGAG TTCATGCGCT TCAAGGTGCA CATGGAGGGC TCCGTGAACG GCCACGAGTT CGAGATCGAG GGCGAGGGCG AGGGCCGCCC CTACGAGGGC ACCCAGACCG CCAAGCTGAA GGTGACCAAG GGTGGCCCCC TGCCCTTCGC CTGGGACATC CTGTCCCCTC AGTTCATGTA CGGCTCCAAG GCCTACGTGA AGCACCCCGC CGACATCCCC GACTACTTGA AGCTGTCCTT CCCCGAGGGC TTCAAGTGGG AGCGCGTGAT GAACTTCGAG GACGGCGGCG TGGTGACCGT GACCCAGGAC TCCTCCCTGC AGGACGGCGA GTTCATCTAC AAGGTGAAGC TGCGCGGCAC CAACTTCCCC TCCGACGGCC CCGTAATGCA GAAGAAGACC ATGGGCTGGG AGGCCTCCTC CGAGCGGATG TACCCCGAGG ACGGCGCCCT GAAGGGCGAG ATCAAGCAGA GGCTGAAGCT GAAGGACGGC GGCCACTACG ACGCTGAGGT CAAGACCACC TACAAGGCCA AGAAGCCCGT GCAGCTGCCC GGCGCCTACA ACGTCAACAT CAAGTTGGAC ATCACCTCCC ACAACGAGGA CTACACCATC GTGGAACAGT ACGAACGCGC CGAGGGCCGC CACTCCACCG GCGGCATGGA CGAGCTGTAC AAGTAGCGGC CGCGACTCTA GATCATAATC AGCCATACCA CATTTGTAGA GGTTTTACTT GCTTTAAAAA ACCTCCCACA CCTCCCCCTG AACCTGAAAC ATAAAATGAA TGCAATTGTT GTTGTTAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTAAG GCGTAAATTG TAAGCGTTAA TATTTTGTTA AAATTCGCGT TAAATTTTTG TTAAATCAGC TCATTTTTTA ACCAATAGGC CGAAATCGGC AAAATCCCTT ATAAATCAAA AGAATAGACC GAGATAGGGT TGAGTGTTGT TCCAGTTTGG AACAAGAGTC CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA AACCGTCTAT CAGGGCGATG GCCCACTACG TGAACCATCA CCCTAATCAA GTTTTTTGGG GTCGAGGTGC CGTAAAGCAC TAAATCGGAA CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG CCGGCGAACG TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG GCAAGTGTAG CGGTCACGCT GCGCGTAACC ACCACACCCG CCGCGCTTAA TGCGCCGCTA CAGGGCGCGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TCCTGAGGCG GAAAGAACCA GCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA GATCGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC CTAGGGGGAG GCTAACTGAA ACACGGAAGG AGACAATACC GGAAGGAACC CGCGCTATGA CGGCAATAAA AAGACAGAAT AAAACGCACG GTGTTGGGTC GTTTGTTCAT AAACGCGGGG TTCGGTCCCA GGGCTGGCAC TCTGTCGATA CCCCACCGAG ACCCCATTGG GGCCAATACG CCCGCGTTTC TTCCTTTTCC CCACCCCACC CCCCAAGTTC GGGTGAAGGC CCAGGGCTCG CAGCCAACGT CGGGGCGGCA GGCCCTGCCA TAGCCTCAGG TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCATGCAT
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