NM_006517.5;AHDS; DXS128; DXS128E; MCT 7; MCT 8; MCT7;MCT8; MRX22; XPCT;
100μg liquid plasmid(Instantly):$91
| name | pCAG-SLC16A2(human)-10×His-FLAG | another name | apCAG-SLC16A2(human)-10×His-FLAG |
| Insert Size (bp) | 1617bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CAG | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pCAG-_CFCH | 5′ sequencing primer | pcaggs-F |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CACCCCCCCC TCCCCCCGAG TTGCTGAGCA CGGCCCGGCT TCGGGTGCGG GGCTCCGTAC GGGGCGTGGC GCGGGGCTCG CCGTGCCGGG CGGGGGGTGG CGGCAGGTGG GGGTGCCGGG CGGGGCGGGG CCGCCTCGGG CCGGGGAGGG CTCGGGGGAG GGGCGCGGCG GCCCCCGGAG CGCCGGCGGC TGTCGAGGCG CGGCGAGCCG CAGCCATTGC CTTTTATGGT AATCGTGCGA GAGGGCGCAG GGACTTCCTT TGTCCCAAAT CTGTGCGGAG CCGAAATCTG GGAGGCGCCG CCGCACCCCC TCTAGCGGGC GCGGGGCGAA GCGGTGCGGC GCCGGCAGGA AGGAAATGGG CGGGGAGGGC CTTCGTGCGT CGCCGCGCCG CCGTCCCCTT CTCCCTCTCC AGCCTCGGGG CTGTCCGCGG GGGGACGGCT GCCTTCGGGG GGGACGGGGC AGGGCGGGGT TCGGCTTCTG GCGTGTGACC GGCGGCTCTA GAGCCTCTGC TAACCATGTT CATGCCTTCT TCTTTTTCCT ACAGCTCCTG GGCAACGTGC TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTATC GATCCGGAGG TACCGCCACC ATGGCGCTGC AAAGCCAGGC GAGCGAGGAA GCAAAGGGGC CCTGGCAGGA GGCAGACCAG GAACAGCAGG AGCCGGTGGG TAGCCCAGAG CCGGAGTCTG AGCCGGAGCC TGAGCCCGAG CCCGAGCCCG TGCCAGTGCC CCCGCCCGAG CCCCAGCCGG AGCCCCAGCC CCTACCGGAC CCCGCACCCC TGCCGGAGCT GGAGTTCGAG TCCGAGCGGG TGCACGAACC CGAGCCCACG CCTACGGTAG AGACCCGCGG CACCGCGCGC GGCTTCCAGC CTCCCGAAGG TGGCTTCGGC TGGGTGGTGG TGTTCGCTGC CACCTGGTGC AACGGCTCCA TCTTCGGCAT CCATAACTCT GTCGGGATCC TCTACTCCAT GCTGCTAGAG GAGGAAAAGG AAAAAAATCG CCAAGTGGAG TTCCAAGCAG CATGGGTCGG AGCCCTCGCG ATGGGTATGA TCTTCTTCTG TTCTCCCATT GTGAGTATAT TCACTGACCG TTTGGGCTGC CGAATCACAG CAACCGCGGG GGCTGCCGTT GCTTTCATTG GCCTCCATAC CAGCTCCTTC ACCAGCTCCC TAAGCCTGCG CTACTTCACC TACGGGATTC TCTTTGGTTG TGGCTGTTCC TTCGCCTTTC AGCCATCCCT CGTCATCCTG GGCCACTACT TTCAACGCCG CCTGGGTCTG GCCAATGGTG TGGTGTCTGC TGGGAGTAGC ATTTTCTCCA TGTCCTTCCC CTTCCTCATC AGAATGCTGG GGGATAAGAT CAAGCTGGCC CAAACCTTCC AGGTGCTGAG TACCTTCATG TTTGTTCTTA TGCTGCTTTC ACTCACCTAC CGGCCCCTCC TGCCCAGCTC CCAGGACACC CCAAGCAAGA GAGGTGTCCG CACCCTGCAC CAGCGCTTTC TGGCTCAGCT CAGGAAGTAC TTCAACATGC GAGTGTTCCG CCAACGCACT TACCGCATCT GGGCCTTCGG AATTGCTGCT GCTGCCCTTG GCTACTTTGT TCCCTATGTA CACCTGATGA AGTATGTGGA GGAGGAGTTC TCAGAAATCA AGGAGACCTG GGTGCTCTTG GTGTGTATTG GGGCTACCTC AGGCCTTGGG CGTCTTGTGT CAGGCCACAT CAGTGACTCC ATCCCTGGAC TTAAGAAGAT CTACTTGCAG GTCCTTTCCT TCCTGCTCCT GGGCCTGATG TCCATGATGA TTCCCCTGTG CCGGGACTTC GGGGGCCTTA TCGTCGTCTG TCTTTTCCTG GGCCTTTGCG ATGGCTTCTT CATCACCATC ATGGCCCCCA TTGCATTTGA GCTGGTGGGC CCAATGCAGG CCTCACAGGC CATTGGCTAC CTCCTGGGCA TGATGGCCCT GCCAATGATT GCTGGGCCCC CCATTGCAGG CCTACTCCGC AACTGTTTTG GGGACTACCA TGTGGCCTTC TACTTTGCCG GTGTGCCCCC CATCATCGGG GCTGTAATCC TCTTCTTCGT CCCTCTGATG CATCAAAGGA TGTTCAAGAA AGAGCAGAGA GATTCCAGCA AGGATAAGAT GTTGGCCCCT GACCCAGACC CCAATGGGGA GCTACTGCCG GGCTCCCCCA ACCCTGAGGA ACCAATCCTC GAGGGCTCTG ATGAGGTTGA TGCTGGCTCT CATCACCATC ACCATCACCA TCACCATCAC GGCTCTGTCG AGGATTACAA GGATGACGAT GACAAGTGAT AAGTCGAGAA GCTTGCTAGC AGATCTTTTT CCCTCTGCCA AAAATTATGG GGACATCATG AAGCCCCTTG AGCATCTGAC TTCTGGCTAA TAAAGGAAAT TTATTTTCAT TGCAATAGTG TGTTGGAATT TTTTGTGTCT CTCACTCGGA AGGACATATG GGAGGGCAAA TCATTTAAAA CATCAGAATG AGTATTTGGT TTAGAGTTTG GCAACATATG CCCATATGCT GGCTGCCATG AACAAAGGTT GGCTATAAAG AGGTCATCAG TATATGAAAC AGCCCCCTGC TGTCCATTCC TTATTCCATA GAAAAGCCTT GACTTGAGGT TAGATTTTTT TTATATTTTG TTTTGTGTTA TTTTTTTCTT TAACATCCCT AAAATTTTCC TTACATGTTT TACTAGCCAG ATTTTTCCTC CTCTCCTGAC TACTCCCAGT CATAGCTGTC CCTCTTCTCT TATGGAGATC CCTCGACCTG CAGCCCAAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CGGATCTGTA ACAACACCAT TTAAATGGAG TGGTTACAAA TGGAGTGGTT AATTAAGACA TGTGAGCAAA AGGCCAGCAA AAGGATCTGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTA GATCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAAGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCGACGGAT CGGGAGATCC CTTAATTAAC AACACCATTT GCTCGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTCGAG GTGAGCCCCA CGTTCTGCTT CACTCTCCCC ATCTCCCCCC CCTCCCCACC CCCAATTTTG TATTTATTTA TTTTTTAATT ATTTTGTGCA GCGATGGGGC GGGGGGGGGG GGGGGGGGCC CCCCCCCAGG CGGGGCGGGG CCGGGGCAAA GGGCCGGGCC GGGCCAAGGC GAAAAGGTGC GGCGGCAGCC AATCAAAGCG GCGCGCTCCG AAGTTCCT
