pCMV-WNK1(human)-Bleo
$157.00
In stock
SKU
PL20782
NM_018979.4;HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1;PSK
100μg liquid plasmid(Instantly):$178
| name | pCMV-WNK1(human)-Bleo | another name | apCMV-WNK1(human)-Bleo |
| Insert Size (bp) | 7149bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Bleo | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pcDNA3.1-ZEO(+) | 5′ sequencing primer | CMV-F |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAC TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTGGTA CCGAGCTCGG ATCCGCCACC ATGTCTGGCG GCGCCGCAGA GAAGCAGAGC AGCACTCCCG GTTCCCTGTT CCTCTCGCCG CCGGCTCCTG CCCCCAAGAA TGGCTCCAGC TCCGATTCCT CCGTGGGGGA GAAACTGGGA GCCGCGGCCG CCGACGCTGT GACCGGCAGG ACCGAGGAGT ACAGGCGCCG CCGCCACACT ATGGACAAGG ACAGCCGTGG GGCGGCCGCG ACCACTACCA CCACTGAGCA CCGCTTCTTC CGCCGGAGCG TCATCTGTGA CTCCAATGCC ACTGCACTGG AGCTTCCCGG CCTTCCTCTT TCCCTGCCCC AGCCCAGCAT CCCCGCGGCT GTCCCGCAGA GTGCTCCACC GGAGCCCCAC CGGGAAGAGA CCGTGACCGC CACCGCCACT TCCCAGGTAG CCCAGCAGCC TCCAGCCGCT GCCGCCCCTG GGGAACAGGC CGTCGCGGGC CCTGCCCCCT CGACTGTCCC CAGCAGTACC AGCAAAGACC GCCCAGTGTC CCAGCCTAGC CTTGTGGGGA GCAAAGAGGA GCCGCCGCCG GCGAGAAGTG GCAGCGGCGG CGGCAGCGCC AAGGAGCCAC AGGAGGAACG GAGCCAGCAG CAGGATGATA TCGAAGAGCT GGAGACCAAG GCCGTGGGAA TGTCTAACGA TGGCCGCTTT CTCAAGTTTG ACATCGAAAT CGGCAGAGGC TCCTTTAAGA CGGTCTACAA AGGTCTGGAC ACTGAAACCA CCGTGGAAGT CGCCTGGTGT GAACTGCAGG ATCGAAAATT AACAAAGTCT GAGAGGCAGA GATTTAAAGA AGAAGCTGAA ATGTTAAAAG GTCTTCAGCA TCCCAATATT GTTAGATTTT ATGATTCCTG GGAATCCACA GTAAAAGGAA AGAAGTGCAT TGTTTTGGTG ACTGAACTTA TGACGTCTGG AACACTTAAA ACGTATCTGA AAAGGTTTAA AGTGATGAAG ATCAAAGTTC TAAGAAGCTG GTGCCGTCAG ATCCTTAAAG GTCTTCAGTT TCTTCATACT CGAACTCCAC CTATCATTCA CCGCGATCTT AAATGTGACA ACATCTTTAT CACCGGCCCT ACTGGCTCAG TCAAGATTGG AGACCTCGGT CTGGCAACCC TGAAGCGGGC TTCTTTTGCC AAGAGTGTGA TAGGTACCCC AGAGTTCATG GCCCCTGAGA TGTATGAGGA GAAATATGAT GAATCCGTTG ACGTTTATGC TTTTGGGATG TGCATGCTTG AGATGGCTAC ATCTGAATAT CCTTACTCGG AGTGCCAAAA TGCTGCACAG ATCTACCGTC GCGTGACCAG TGGGGTGAAG CCAGCCAGTT TTGACAAAGT AGCAATTCCT GAAGTGAAGG AAATTATTGA AGGATGCATA CGACAAAACA AAGATGAAAG ATATTCCATC AAAGACCTTT TGAACCATGC CTTCTTCCAA GAGGAAACAG GAGTACGGGT AGAATTAGCA GAAGAAGATG ATGGAGAAAA AATAGCCATA AAATTATGGC TACGTATTGA AGATATTAAG AAATTAAAGG GAAAATACAA AGATAATGAA GCTATTGAGT TTTCTTTTGA TTTAGAGAGA GATGTCCCAG AAGATGTTGC ACAAGAAATG GTAGAGTCTG GGTATGTCTG TGAAGGTGAT CACAAGACCA TGGCTAAAGC TATCAAAGAC AGAGTATCAT TAATTAAGAG GAAACGAGAG CAGCGGCAGT TGGTACGGGA GGAGCAAGAA AAAAAAAAGC AGGAAGAGAG CAGTCTCAAA CAGCAGGTAG AACAATCCAG TGCTTCCCAG ACAGGAATCA AGCAGCTCCC TTCTGCTAGC ACCGGCATAC CTACTGCTTC TACCACTTCA GCTTCAGTTT CTACACAAGT AGAACCTGAA GAACCTGAGG CAGATCAACA TCAACAACTA CAGTACCAGC AACCCAGTAT ATCTGTGTTA TCTGATGGGA CGGTTGACAG TGGTCAGGGA TCCTCTGTCT TCACAGAATC TCGAGTGAGC AGCCAACAGA CAGTTTCATA TGGTTCCCAA CATGAACAGG CACATTCTAC AGGCACAGTC CCAGGGCATA TACCTTCTAC TGTCCAAGCA CAGTCTCAGC CCCATGGGGT ATATCCACCC TCAAGTGTGG CACAGGGGCA GAGCCAGGGT CAGCCATCCT CAAGTAGCTT AACAGGGGTT TCATCTTCCC AACCCATACA ACATCCTCAG CAGCAGCAGG GAATACAGCA GACAGCCCCT CCTCAACAGA CAGTGCAGTA TTCACTTTCA CAGACATCAA CCTCCAGTGA GGCCACTACT GCACAGCCAG TGAGTCAGCC TCAAGCTCCA CAAGTCTTGC CTCAAGTATC AGCTGGAAAA CAGCTTCCAG TTTCCCAGCC AGTACCAACT ATCCAAGGCG AACCTCAGAT CCCAGTTGCG ACACAACCCT CGGTTGTTCC AGTCCACTCT GGTGCTCATT TCCTTCCAGT GGGACAGCCG CTCCCTACTC CCTTGCTCCC TCAGTACCCT GTCTCTCAGA TTCCCATATC AACTCCTCAT GTGTCTACGG CTCAGACAGG TTTCTCATCC CTTCCCATCA CAATGGCAGC TGGCATTACT CAGCCTCTGC TCACGTTGGC TTCATCTGCT ACAACAGCTG CGATCCCGGG GGTATCAACT GTGGTTCCTA GTCAGCTTCC AACCCTTCTG CAGCCTGTGA CTCAGCTGCC AAGTCAGGTT CACCCACAGC TCCTACAACC AGCAGTTCAG TCCATGGGAA TACCAGCTAA CCTTGGACAA GCTGCTGAGG TTCCACTTTC CTCTGGAGAT GTTCTGTACC AGGGCTTCCC ACCTCGACTG CCACCACAGT ACCCAGGAGA TTCAAATATT GCTCCCTCTT CCAACGTGGC TTCTGTTTGC ATCCATTCTA CAGTCCTATC CCCTCCCATG CCGACAGAAG TACTGGCTAC ACCTGGGTAC TTTCCCACAG TGGTGCAGCC TTATGTGGAA TCAAATCTTT TAGTTCCTAT GGGTGGTGTA GGAGGACAGG TTCAAGTGTC CCAGCCAGGA GGGAGTTTAG CACAAGCCCC CACTACATCC TCCCAGCAAG CAGTTTTGGA GAGTACTCAG GGAGTCTCTC AGGTTGCTCC TGCAGAGCCA GTTGCAGTAG CACAGACCCA AGCTACCCAG CCGACCACTT TGGCTTCCTC TGTAGACAGT GCACATTCAG ATGTTGCTTC AGGTATGAGT GATGGCAATG AGAACGTCCC ATCTTCCAGT GGAAGGCATG AAGGAAGAAC TACAAAACGG CATTACCGAA AATCTGTAAG GAGTCGCTCT CGACATGAAA AAACTTCACG CCCAAAATTA AGAATTTTGA ATGTTTCAAA TAAAGGAGAC CGAGTAGTAG AATGTCAATT AGAGACTCAT AATAGGAAAA TGGTTACATT CAAATTTGAC CTAGATGGTG ACAACCCCGA GGAGATAGCA ACAATTATGG TGAACAATGA CTTTATTCTA GCAATAGAGA GAGAGTCGTT TGTGGATCAA GTGCGAGAAA TTATTGAAAA AGCTGATGAA ATGCTCAGTG AGGATGTCAG TGTGGAACCA GAGGGTGATC AGGGATTGGA GAGTCTACAA GGAAAGGATG ACTATGGCTT TTCAGGTTCT CAGAAATTGG AAGGAGAGTT CAAACAACCA ATTCCTGCGT CTTCCATGCC ACAGCAAATA GGCATTCCTA CCAGTTCTTT AACTCAAGTT GTTCATTCTG CGGGAAGGCG GTTTATAGTG AGTCCTGTGC CAGAAAGCCG ATTACGAGAA TCAAAAGTTT TCCCCAGTGA AATAACAGAT ACAGTTGCTG CCTCTACAGC TCAGAGCCCT GGAATGAACT TGTCTCACTC TGCATCATCC CTTAGTCTAC AACAGGCCTT TTCTGAACTT AGACGTGCCC AAATGACAGA AGGACCCAAC ACAGCACCTC CAAACTTTAG TCATACAGGA CCAACATTTC CAGTAGTACC TCCTTTCTTA AGTAGCATTG CTGGAGTCCC AACCACAGCA GCAGCCACAG CACCAGTCCC TGCAACAAGC AGCCCTCCTA ATGACATTTC CACATCAGTA ATTCAGTCTG AGGTTACAGT GCCCACTGAA GAGGGGATTG CTGGAGTTGC CACCAGCACA GGTGTGGTAA CTTCAGGTGG TCTCCCCATA CCACCTGTGT CTGAATCACC AGTACTTTCC AGCGTAGTTT CAAGTATCAC AATACCTGCA GTTGTCTCAA TATCTACTAC ATCCCCGTCA CTTCAAGTCC CCACATCCAC ATCTGAGATC GTTGTTTCTA GTACAGCACT GTATCCTTCA GTAACAGTTT CAGCAACTTC AGCCTCTGCA GGGGGCAGTA CTGCTACCCC AGGTCCTAAG CCTCCAGCTG TAGTATCTCA GCAGGCAGCA GGCAGCACTA CTGTGGGAGC CACATTAACA TCAGTTTCTA CCACCACTTC ATTCCCAAGC ACAGCTTCAC AGCTGTGCAT TCAGCTTAGC AGCAGTACTT CTACTCCTAC TTTAGCTGAA ACCGTGGTAG TTAGCGCACA CTCACTAGAT AAGACATCTC ATAGCAGTAC AACTGGATTG GCTTTCTCCC TCTCTGCACC ATCTTCCTCT TCCTCTCCTG GAGCAGGAGT GTCTAGTTAT ATTTCTCAGC CTGGTGGGCT GCATCCTTTG GTCATTCCAT CAGTGATAGC TTCTACTCCT ATTCTTCCCC AAGCAGCAGG ACCTACTTCT ACACCTTTAT TACCCCAAGT ACCTAGTATC CCACCCTTGG TACAGCCTGT TGCCAATGTG CCTGCTGTAC AGCAGACACT AATTCATAGT CAGCCTCAAC CAGCTTTGCT TCCCAACCAG CCCCATACTC ATTGTCCTGA AGTAGATTCT GATACACAAC CCAAAGCTCC TGGAATTGAT GACATAAAGA CTCTAGAAGA AAAGCTGCGG TCTCTGTTCA GTGAACACAG CTCATCTGGA GCTCAGCATG CCTCTGTCTC ACTGGAGACC TCACTAGTCA TAGAGAGCAC TGTCACACCA GGCATCCCAA CTACTGCTGT TGCACCAAGC AAACTCCTGA CTTCTACCAC AAGTACTTGC TTACCACCAA CCAATTTACC ACTAGGAACA GTTGCTTTGC CAGTTACACC AGTGGTCACA CCTGGGCAAG TTTCTACCCC AGTCAGCACT ACTACATCAG GAGTGAAACC TGGAACTGCT CCCTCCAAGC CACCTCTAAC TAAGGCTCCG GTGCTGCCAG TGGGTACTGA ACTTCCAGCA GGTACTCTAC CCAGCGAGCA GCTGCCACCT TTTCCAGGAC CTTCTCTAAC CCAGTCCCAG CAACCTCTAG AGGATCTTGA TGCTCAATTG AGAAGAACAC TTAGTCCAGA GATGATCACA GTGACTTCTG CGGTTGGTCC TGTGTCCATG GCGGCTCCAA CAGCAATCAC AGAAGCAGGA ACACAGCCTC AGAAGGGTGT TTCTCAAGTC AAAGAAGGCC CTGTCCTAGC AACTAGTTCA GGAGCTGGTG TTTTTAAGAT GGGACGATTT CAGGTTTCTG TTGCAGCAGA CGGTGCCCAG AAAGAGGGTA AAAATAAGTC AGAAGATGCA AAGTCTGTTC ATTTTGAATC CAGCACCTCA GAGTCCTCAG TGCTATCAAG TAGTAGTCCA GAGAGTACCT TGGTGAAACC AGAGCCGAAT GGCATAACCA TCCCTGGTAT CTCTTCAGAT GTGCCAGAGA GTGCCCACAA AACTACTGCC TCAGAGGCAA AGTCAGACAC TGGGCAGCCT ACCAAGGTTG GACGTTTTCA GGTGACAACT ACAGCAAACA AAGTGGGTCG TTTCTCTGTA TCAAAAACTG AGGACAAGAT CACTGACACA AAGAAAGAAG GACCAGTGGC ATCTCCTCCT TTTATGGATT TGGAACAAGC TGTTCTTCCT GCTGTGATAC CAAAGAAAGA GAAGCCTGAA CTGTCAGAGC CTTCACATCT AAATGGGCCG TCTTCTGACC CGGAGGCCGC TTTTTTAAGT AGGGATGTGG ATGATGGTTC CGGTAGTCCA CACTCGCCCC ATCAGCTGAG CTCAAAGAGC CTTCCTAGCC AGAATCTAAG TCAAAGCCTT AGTAATTCAT TTAACTCCTC TTACATGAGT AGCGACAATG AGTCAGATAT CGAAGATGAA GACTTAAAGT TAGAGCTGCG ACGACTACGA GATAAACATC TCAAAGAGAT TCAGGACCTG CAGAGTCGCC AGAAGCATGA AATTGAATCT TTGTATACCA AACTGGGCAA GGTGCCCCCT GCTGTTATTA TTCCCCCAGC TGCTCCCCTT TCAGGGAGAA GACGACGACC CACTAAAAGC AAAGGCAGCA AATCTAGTCG AAGCAGTTCC TTGGGGAATA AAAGCCCCCA GCTTTCAGGT AACCTGTCTG GTCAGAGTGC AGCTTCAGTC TTGCACCCCC AGCAGACCCT CCACCCTCCT GGCAACATCC CAGAGTCCGG GCAGAATCAG CTGTTACAGC CCCTTAAGCC ATCTCCCTCC AGTGACAACC TCTATTCAGC CTTCACCAGT GATGGTGCCA TTTCAGTACC AAGCCTTTCT GCTCCAGGTC AAGGAACCAG CAGCACAAAC ACTGTTGGGG CAACAGTGAA CAGCCAAGCC GCCCAAGCTC AGCCTCCTGC CATGACGTCC AGCAGGAAGG GCACATTCAC AGATGACTTG CACAAGTTGG TAGACAATTG GGCCCGAGAT GCCATGAATC TCTCAGGCAG GAGAGGAAGC AAAGGGCACA TGAATTACGA GGGCCCTGGA ATGGCAAGGA AGTTCTCTGC ACCTGGGCAA CTGTGCATCT CCATGACCTC GAACCTGGGT GGCTCTGCCC CCATCTCTGC AGCATCAGCT ACCTCTCTAG GTCACTTCAC CAAGTCTATG TGCCCCCCAC AGCAGTATGG CTTTCCAGCT ACCCCATTTG GCGCTCAATG GAGTGGGACG GGTGGCCCAG CACCACAGCC ACTTGGCCAG TTCCAACCTG TGGGAACTGC CTCCTTGCAG AATTTCAACA TCAGCAATTT GCAGAAATCC ATCAGCAACC CCCCAGGCTC CAACCTGCGG ACCACTTAGG AATTCTGCAG ATATCCAGCA CAGTGGCGGC CGCTCGAGTC TAGAGGGCCC GTTTAAACCC GCTGATCAGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG GGATGCGGTG GGCTCTATGG CTTCTGAGGC GGAAAGAACC AGCTGGGGCT CTAGGGGGTA TCCCCACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGCATCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGGGGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTAATTCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAG CACGTGTTGA CAATTAATCA TCGGCATAGT ATATCGGCAT AGTATAATAC GACAAGGTGA GGAACTAAAC CATGGCCAAG TTGACCAGTG CCGTTCCGGT GCTCACCGCG CGCGACGTCG CCGGAGCGGT CGAGTTCTGG ACCGACCGGC TCGGGTTCTC CCGGGACTTC GTGGAGGACG ACTTCGCCGG TGTGGTCCGG GACGACGTGA CCCTGTTCAT CAGCGCGGTC CAGGACCAGG TGGTGCCGGA CAACACCCTG GCCTGGGTGT GGGTGCGCGG CCTGGACGAG CTGTACGCCG AGTGGTCGGA GGTCGTGTCC ACGAACTTCC GGGACGCCTC CGGGCCGGCC ATGACCGAGA TCGGCGAGCA GCCGTGGGGG CGGGAGTTCG CCCTGCGCGA CCCGGCCGGC AACTGCGTGC ACTTCGTGGC CGAGGAGCAG GACTGACACG TGCTACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CAATGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTC
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