pCMV-TRIP12(human-toxic-opt)-3×Myc-Neo
$142.00
In stock
SKU
PL20727
NM_001284214.2,MRD49/TRIP-12/TRIPC/ULF;
100μg liquid plasmid(Instantly):$163
| name | pCMV-TRIP12(human-toxic-opt)-3×Myc-Neo | another name | apCMV-TRIP12(human-toxic-opt)-3×Myc-Neo |
| Insert Size (bp) | 6120bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pCMV-TRIP12(human)-3×Myc-Neo | 5′ sequencing primer | CMV-F |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCATGTC CAACCGGCCT AATAACAATC CAGGGGGGTC ACTGCGACGT TCACAGAGGA ACACTGCCGG GGCCCAACCA CAAGACGACT CAATAGGAGG AAGGTCACAT TTAGGGCAGG CAAAACATAA GGGATATAGC CCTCCTGAGA GTAGAAAATC TAATTCTAAG GCACCCAAAG TGCAGTCTAA TACTACTTCT GAACTGTCAA GGGGACACCT TTCTAAAAGA AGCTGCAGTT CATCATCTGC TGTGATAGTT CCACAACCAG AGGATCCAGA CAGAGCCAAT ACTTCAGAAA GACAAAAAAC GGGGCAGGTG CCTAAGAAAG ACAATTCTCG AGGAGTGAAG CGCAGTGCTA GTCCAGACTA CAACAGGACC AATTCTCCTA GCTCTGCAAA AAAACCAAAA GCACTTCAGC ATACTGAATC TCCCTCAGAA ACAAATAAGC CACATAGTAA GTCAAAGAAG AGACATTTAG ACCAGGAGCA ACAACTGAAA TCTGCACAAT CACCATCAAC AAGCAAGGCT CATACCAGGA AGAGTGGGGC CACTGGCGGT TCACGGAGTC AGAAAAGAAA AAGGACAGAG AGTTCTTGTG TAAAGAGTGG CTCCGGGTCT GAATCAACTG GTGCAGAAGA GAGATCTGCG AAACCTACCA AGCTGGCTTC AAAATCAGCC ACCTCAGCCA AAGCTGGGTG TAGCACCATC ACTGATTCTT CTTCTGCTGC CTCTACTTCC TCCTCGTCTT CTGCTGTAGC CTCGGCCTCC TCCACTGTAC CACCAGGTGC CAGAGTGAAA CAAGGAAAAG ATCAGAACAA GGCCAGGCGT TCCCGTTCAG CGTCCAGTCC CAGCCCCAGA AGAAGTAGCA GGGAAAAGGA ACAGAGTAAA ACTGGTGGCT CTTCAAAATT TGATTGGGCT GCTCGTTTCA GCCCTAAAGT TAGCCTTCCT AAAACAAAAC TGTCTCTTCC AGGGTCTTCT AAGTCAGAGA CATCAAAACC TGGACCTTCT GGATTACAGG CCAAATTAGC AAGTTTAAGA AAATCTACGA AGAAACGCAG TGAGTCTCCA CCTGCTGAGC TCCCCAGTTT GAGGCGGAGC ACACGCCAAA AGACCACGGG CTCCTGTGCT AGTACCAGTC GGCGAGGCTC TGGCCTGGGC AAAAGAGGAG CAGCTGAAGC TCGTCGACAG GAGAAAATGG CAGACCCTGA AAGCAACCAG GAGGCAGTAA ATTCTTCAGC TGCTCGGACA GATGAAGCTC CCCAAGGAGC TGCAGCCTCT AGTTCTGTTG CAGGGGCTGT TGGCATGACC ACCTCTGGGG AGAGTGAATC AGATGATTCC GAGATGGGAC GTTTGCAAGC TTTGTTAGAG GCAAGGGGTC TTCCCCCTCA CCTATTTGGT CCTCTTGGTC CTCGGATGTC ACAGCTTTTC CATAGAACAA TTGGAAGTGG AGCTAGTTCT AAGGCCCAGC AGCTACTACA AGGATTGCAA GCCAGTGATG AAAGTCAACA GCTTCAGGCA GTTATTGAGA TGTGTCAGTT ACTGGTCATG GGAAATGAGG AGACACTGGG AGGGTTTCCT GTCAAGAGTG TTGTTCCAGC TTTGATTACG TTACTTCAGA TGGAGCACAA TTTTGATATT ATGAACCATG CTTGTCGAGC CTTAACATAC ATGATGGAAG CACTTCCTCG ATCTTCTGCT GTTGTAGTAG ATGCTATTCC TGTCTTTTTA GAAAAGCTGC AAGTTATTCA GTGTATTGAT GTGGCAGAGC AGGCCTTGAC TGCCTTGGAG ATGTTGTCAC GGAGACATAG TAAAGCCATT CTACAGGCGG GTGGTTTGGC AGACTGCTTG CTGTACCTAG AATTCTTCAG CATAAATGCC CAAAGAAATG CATTAGCAAT TGCAGCTAAT TGCTGCCAGA GTATCACGCC AGATGAATTT CATTTTGTGG CAGATTCACT CCCATTGCTA ACCCAAAGGC TAACACATCA GGATAAAAAG TCAGTAGAAA GCACTTGCCT TTGTTTTGCA CGCCTAGTGG ACAACTTCCA GCATGAGGAG AATTTACTCC AGCAGGTTGC TTCCAAAGAT CTGCTTACAA ATGTTCAACA GCTGTTGGTA GTGACTCCAC CCATTTTAAG TTCTGGGATG TTCATAATGG TGGTGAGAAT GTTTAGCCTT ATGTGCTCAA ATTGCCCTAC CCTGGCTGTT CAGCTGATGA AGCAGAACAT CGCAGAGACC CTTCATTTCC TGTTGTGTGG CGCTTCCAAC GGCAGCTGCC AGGAGCAGAT CGATTTGGTG CCCCGCTCTC CACAGGAATT GTACGAGCTG ACTAGCCTTA TCTGTGAGCT GATGCCCTGT CTGCCAAAGG AGGGAATATT CGCGGTCGAC ACAATGCTGA AGAAAGGCAA TGCCCAGAAC ACTGATGGGG CTATCTGGCA ATGGAGAGAT GATAGAGGCT TGTGGCACCC GTATAACCGG ATCGATAGCC GCATAATCGA GCAGATCAAT GAGGATACCG GCACAGCCCG CGCCATACAG AGGAAGCCCA ATCCACTCGC CAACTCCAAT ACTAGTGGAT ACAGCGAAAG TAAGAAGGAC GACGCACGGG CTCAACTCAT GAAGGAGGAC CCGGAGCTTG CAAAGAGTTT CATCAAGACT CTCTTCGGCG TACTCTACGA GGTGTATTCT TCCAGTGCCG GTCCAGCTGT CAGGCACAAA TGTCTCAGGG CCATTCTCCG AATTATCTAT TTCGCCGATG CTGAGCTGCT CAAAGACGTC CTGAAGAACC ATGCCGTGTC TAGCCACATC GCTTCAATGC TGTCAAGCCA GGACCTGAAA ATCGTGGTTG GCGCCCTGCA GATGGCAGAG ATCTTGATGC AGAAGCTGCC CGATATCTTC TCCGTCTACT TCCGCAGAGA AGGGGTGATG CACCAGGTGA AACACCTGGC CGAGTCTGAA TCTCTGCTGA CATCTCCCCC AAAGGCATGT ACCAATGGAA GCGGATCCAT GGGTTCAACC ACATCTGTGT CCAGTGGTAC CGCCACCGCA GCCACACATG CTGCAGCAGA TCTCGGGAGC CCATCTCTTC AGCACTCAAG AGATGACAGT CTTGATCTGA GCCCGCAGGG CAGGCTGTCA GATGTGCTCA AACGGAAGCG CCTGCCTAAG AGGGGTCCAA GAAGGCCAAA GTACTCACCT CCAAGAGATG ATGACAAAGT AGACAATCAA GCTAAAAGCC CCACCACTAC TCAGTCACCT AAATCTTCTT TCCTGGCAAG CTTGAATCCA AAAACATGGG GAAGGTTAAG TACACAGTCC AACAGCAACA ACATTGAGCC AGCACGGACT GCGGGAGGTA GTGGCCTTGC CAGGGCTGCC TCAAAGGATA CCATCTCCAA TAATAGAGAA AAAATTAAAG GTTGGATTAA GGAGCAGGCA CATAAATTTG TAGAACGTTA TTTCAGTTCT GAGAATATGG ATGGAAGCAA CCCTGCATTG AATGTCCTTC AGAGACTTTG TGCTGCAACC GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA GTCTCAGAGT CAGATGTTTC ATCATTTGAA ATCCAACATA GTGGATTTGT GAAGCAGCTG TTGCTTTATT TGACATCTAA AAGTGAAAAG GATGCTGTGA GCAGAGAGAT CAGATTAAAG CGATTTCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCCAT TGGAAGAGTG GAACCAGTGG GTAATGCACC TTTGTTGGCA TTAGTTCACA AGATGAACAA CTGCCTCAGC CAGATGGAAC AATTTCCAGT CAAAGTACAT GATTTCCCTA GTGGAAATGG GACAGGAGGC AGCTTTTCTC TCAACAGAGG ATCACAGGCT TTAAAATTTT TCAACACACA TCAATTAAAA TGCCAGTTAC AAAGGCATCC AGACTGTGCA AATGTGAAGC AGTGGAAGGG TGGACCTGTC AAGATTGACC CTCTGGCTTT GGTACAAGCC ATCGAGAGAT ACCTTGTAGT TAGAGGGTAT GGAAGAGTAA GAGAAGATGA TGAAGACAGC GATGACGATG GATCAGATGA GGAAATAGAT GAGTCTCTGG CTGCTCAGTT CCTAAATTCA GGAAATGTAA GACACAGGCT GCAGTTTTAT ATTGGAGAAC ATTTGCTGCC GTATAACATG ACTGTGTATC AGGCAGTACG GCAGTTTAGT ATACAGGCTG AAGATGAAAG AGAATCCACA GATGATGAGA GCAATCCTCT AGGCAGAGCT GGTATTTGGA CAAAGACTCA TACAATATGG TATAAACCTG TGAGAGAGGA TGAAGAAAGT AATAAAGATT GTGTTGGTGG TAAAAGAGGA AGAGCCCAAA CAGCTCCAAC GAAAACTTCC CCTAGAAATG CAAAAAAGCA TGATGAGTTA TGGCACGATG GAGTGTGCCC ATCAGTATCA AATCCTTTAG AAGTTTACCT CATTCCCACA CCACCTGAAA ATATAACATT TGAAGACCCG TCATTAGATG TGATCCTTCT TTTAAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAC TTGTATGATA ATGCAATGTG CAAGGAAATT ATTCCAACTA GTGAATTTAT TAACAGTAAG TTAACAGCAA AAGCAAATAG GCAACTTCAA GATCCTTTAG TAATCATGAC AGGAAACATC CCAACATGGC TTACTGAGCT AGGAAAAACC TGCCCATTTT TCTTTCCTTT TGATACCCGG CAAATGCTTT TTTATGTAAC TGCATTTGAT CGGGACCGAG CAATGCAAAG ATTACTTGAT ACCAACCCAG AAATCAACCA GTCTGATTCT CAAGATAGCA GAGTTGCACC TAGATTGGAT AGAAAAAAAC GTACTGTGAA CCGAGAGGAG CTGCTGAAAC AGGCGGAGTC TGTGATGCAG GACCTCGGCA GCTCACGGGC CATGTTAGAA ATCCAGTATG AAAATGAGGT TGGTACAGGT CTTGGGCCTA CACTGGAGTT TTATGCGCTT GTATCTCAGG AACTACAGAG AGCTGACTTG GGTCTTTGGA GAGGTGAAGA AGTAACTCTT AGCAATCCAA AAGGGAGCCA AGAAGGGACC AAGTATATTC AAAACCTCCA GGGCCTGTTT GCGCTTCCCT TTGGTAGGAC AGCAAAGCCA GCTCATATCG CAAAGGTTAA GATGAAGTTT CGCTTCTTAG GAAAATTAAT GGCCAAGGCT ATCATGGATT TCAGATTGGT GGACCTTCCC CTTGGCTTAC CCTTTTATAA ATGGATGCTA CGGCAAGAAA CTTCACTGAC ATCACACGAT TTGTTTGACA TCGACCCAGT TGTAGCCAGA TCAGTTTATC ACCTAGAAGA CATTGTCAGA CAGAAGAAAA GACTTGAACA AGATAAATCC CAGACCAAAG AGAGTCTACA GTATGCATTA GAAACCTTGA CTATGAATGG CTGCTCAGTT GAAGATCTAG GACTGGATTT CACTCTGCCA GGGTTTCCCA ATATCGAACT GAAGAAAGGA GGGAAGGATA TACCAGTCAC TATCCACAAT TTAGAGGAGT ATCTAAGACT GGTTATATTC TGGGCACTAA ATGAAGGCGT TTCTAGGCAA TTTGATTCGT TCAGAGATGG ATTTGAATCA GTCTTCCCAC TCAGTCATCT TCAGTACTTC TACCCGGAGG AACTGGATCA GCTCCTTTGT GGCAGTAAAG CAGACACTTG GGATGCAAAG ACACTGATGG AATGCTGTAG GCCTGATCAT GGTTATACTC ATGACAGTCG GGCTGTGAAG TTTTTGTTTG AGATTCTCAG TAGTTTTGAT AATGAGCAGC AGAGGTTATT TCTCCAGTTT GTGACTGGTA GCCCAAGATT GCCTGTTGGA GGATTCCGGA GTTTGAATCC ACCTTTGACA ATTGTCCGAA AGACGTTTGA ATCAACAGAA AACCCAGATG ACTTCTTGCC CTCTGTAATG ACTTGTGTGA ACTATCTTAA GTTGCCGGAC TATTCAAGCA TTGAGATAAT GCGTGAAAAA CTGTTGATAG CAGCAAGAGA AGGGCAGCAG TCGTTCCATC TTTCCCTCGA GGAACAAAAA CTAATATCAG AGGAAGATTT GGAACAGAAA CTAATCAGTG AAGAAGATTT AGAGCAAAAG TTAATTTCCG AGGAGGACTT ATAGTGAGGG CCCGATATCT CGACAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAG CTGACGTCCT TTCCATGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCCTGGAAA CGGGGGAGGC TAACTGAAAC ACGGAAGGAG ACAATACCGG AAGGAACCCG CGCTATGACG GCAATAAAAA GACAGAATAA AACGCACGGG TGTTGGGTCG TTTGTTCATA AACGCGGGGT TCGGTCCCAG GGCTGGCACT CTGTCGATAC CCCACCGAGA CCCCATTGGG GCCAATACGC CCGCGTTTCT TCCTTTTCCC CACCCCACCC CCCAAGTTCG GGTGAAGGCC CAGGGCTCGC AGCCAACGTC GGGGCGGCAG GCCCTGCCAT AGCAGATCTG CGCAGCTGGG GCTCTAGGGG GTATCCCCAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGCATCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGG GGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTAAT TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCAATGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCGACGGA TCGGGAGATC TCCCGATCCC CTATGGTCGA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATCTGCTC CCTGCTTGTG TGTTGGAGGT CGCTGAGTAG TGCGCGAGCA AAATTTAAGC TACAACAAGG CAAGGCTTGA CCGACAATTG CATGAAGAAT CTGCTTAGG
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