pET28a_T7-ARG1-LUX
$70.00
In stock
SKU
PL20649
SC114672
100μg liquid plasmid(Instantly):$113
| name | pET28a_T7-ARG1-LUX | another name | apET28a_T7-ARG1-LUX |
| Insert Size (bp) | 1650bp | Plasmid host | Escherichia coli |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Bacillus,Fishy algae | Prokaryotic resistance | Kan |
| Selective marker | Fluorescent labeling | LuX | |
| Promoter | T7 | Replicon | pBR322 |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pET28a_T7-ARG1 | 5′ sequencing primer | T7 |
| 3′ sequencing primer | Copy number | Low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGCTT ACAATTTAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA ATTAATTCTT AGAAAAACTC ATCGAGCATC AAATGAAACT GCAATTTATT CATATCAGGA TTATCAATAC CATATTTTTG AAAAAGCCGT TTCTGTAATG AAGGAGAAAA CTCACCGAGG CAGTTCCATA GGATGGCAAG ATCCTGGTAT CGGTCTGCGA TTCCGACTCG TCCAACATCA ATACAACCTA TTAATTTCCC CTCGTCAAAA ATAAGGTTAT CAAGTGAGAA ATCACCATGA GTGACGACTG AATCCGGTGA GAATGGCAAA AGTTTATGCA TTTCTTTCCA GACTTGTTCA ACAGGCCAGC CATTACGCTC GTCATCAAAA TCACTCGCAT CAACCAAACC GTTATTCATT CGTGATTGCG CCTGAGCGAG ACGAAATACG CGATCGCTGT TAAAAGGACA ATTACAAACA GGAATCGAAT GCAACCGGCG CAGGAACACT GCCAGCGCAT CAACAATATT TTCACCTGAA TCAGGATATT CTTCTAATAC CTGGAATGCT GTTTTCCCGG GGATCGCAGT GGTGAGTAAC CATGCATCAT CAGGAGTACG GATAAAATGC TTGATGGTCG GAAGAGGCAT AAATTCCGTC AGCCAGTTTA GTCTGACCAT CTCATCTGTA ACATCATTGG CAACGCTACC TTTGCCATGT TTCAGAAACA ACTCTGGCGC ATCGGGCTTC CCATACAATC GATAGATTGT CGCACCTGAT TGCCCGACAT TATCGCGAGC CCATTTATAC CCATATAAAT CAGCATCCAT GTTGGAATTT AATCGCGGCC TAGAGCAAGA CGTTTCCCGT TGAATATGGC TCATAACACC CCTTGTATTA CTGTTTATGT AAGCAGACAG TTTTATTGTT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GAGATCCCGG TGCCTAATGA GTGAGCTAAC TTACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCCAGGGTG GTTTTTCTTT TCACCAGTGA GACGGGCAAC AGCTGATTGC CCTTCACCGC CTGGCCCTGA GAGAGTTGCA GCAAGCGGTC CACGCTGGTT TGCCCCAGCA GGCGAAAATC CTGTTTGATG GTGGTTAACG GCGGGATATA ACATGAGCTG TCTTCGGTAT CGTCGTATCC CACTACCGAG ATATCCGCAC CAACGCGCAG CCCGGACTCG GTAATGGCGC GCATTGCGCC CAGCGCCATC TGATCGTTGG CAACCAGCAT CGCAGTGGGA ACGATGCCCT CATTCAGCAT TTGCATGGTT TGTTGAAAAC CGGACATGGC ACTCCAGTCG CCTTCCCGTT CCGCTATCGG CTGAATTTGA TTGCGAGTGA GATATTTATG CCAGCCAGCC AGACGCAGAC GCGCCGAGAC AGAACTTAAT GGGCCCGCTA ACAGCGCGAT TTGCTGGTGA CCCAATGCGA CCAGATGCTC CACGCCCAGT CGCGTACCGT CTTCATGGGA GAAAATAATA CTGTTGATGG GTGTCTGGTC AGAGACATCA AGAAATAACG CCGGAACATT AGTGCAGGCA GCTTCCACAG CAATGGCATC CTGGTCATCC AGCGGATAGT TAATGATCAG CCCACTGACG CGTTGCGCGA GAAGATTGTG CACCGCCGCT TTACAGGCTT CGACGCCGCT TCGTTCTACC ATCGACACCA CCACGCTGGC ACCCAGTTGA TCGGCGCGAG ATTTAATCGC CGCGACAATT TGCGACGGCG CGTGCAGGGC CAGACTGGAG GTGGCAACGC CAATCAGCAA CGACTGTTTG CCCGCCAGTT GTTGTGCCAC GCGGTTGGGA ATGTAATTCA GCTCCGCCAT CGCCGCTTCC ACTTTTTCCC GCGTTTTCGC AGAAACGTGG CTGGCCTGGT TCACCACGCG GGAAACGGTC TGATAAGAGA CACCGGCATA CTCTGCGACA TCGTATAACG TTACTGGTTT CACATTCACC ACCCTGAATT GACTCTCTTC CGGGCGCTAT CATGCCATAC CGCGAAAGGT TTTGCGCCAT TCGATGGTGT CCGGGATCTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAATT AATACGACTC ACTATAGGGG AATTGTGAGC GGATAACAAT TCCAAGCTTC GCCAGGGTTT TCCCAGTCGA GCTCATGACT CATATTTATA GTTATTCTCC TCATTTCCCA AGTTTCCGAC AACAAATAAC AAGGAATTAA AAAAATGGCA GTTGAAAAAA CCAATTCTTC CTCCAGCTTG GCAGAAGTTA TTGATAGAAT CCTCGACAAA GGTATCGTAA TTGACGCTTG GGTTCGTGTT TCCTTAGTTG GTATCGAACT ACTAGCAATT GAAGCTCGGA TCGTTATCGC TTCCGTTGAA ACCTACTTGA AGTATGCTGA AGCAGTTGGT TTGACCCAAT CAGCAGCAGT ACCTGCTTAA TTTAATTAAG CAAGTTACCA AGTAGGAGAA TAACCATGAC TCATATTTAT AGTTATTCTC CTCATTTCCC AAGTTTCCGA CAACAAATAA CAAGGAATTA AAAAAATGGC AGTTGAAAAA ACCAATTCTT CCTCCAGCTT GGCAGAAGTT ATTGATAGAA TCCTCGACAA AGGTATCGTA ATTGACGCTT GGGTTCGTGT TTCCTTAGTT GGTATCGAAC TACTAGCAAT TGAAGCTCGG ATCGTTATCG CTTCCGTTGA AACCTACTTG AAGTATGCTG AAGCAGTTGG TTTGACCCAA TCAGCAGCAG TACCTGCTTA ATTTAATTAA GCTCCATAAT ATCAGGATTA ATTAATCCTG ATATTGGTTC TGCTTTTTTT AATTATCTAG ATATTTGGAA ATCAACGCTT CTCAAAGGAT ATTACAAAAA AGTACGAATC CTTATAATGT AGTACCTGGA GAAGCAATTT CTGTTAGGTC TGGGAGCTCC CATGGGATCG TTATCCCGCG GCCAAGCTTG CTAGCGCTAA TTTACTATTT TTTGGAGAAC TTCATGATTT CTTTAATGGC AAAAATCCGG CAAGAACATC AGTCAATAGC AGAGAAAGTG GCTGAACTAT CTCTTGAGAC CAGAGAATTC TTGTCCGTCA CGACAGCGAA AAGACAAGAG CAAGCTGAAA AACAAGCTCA AGAACTGCAA GCATTCTACA AGGATCTTCA GGAAACAAGT CAGCAGTTTT TATCAGAAAC AGCCCAAGCC AGAATTGCTC AAGCTGAAAA ACAAGCTCAA GAACTGTTAG CATTCCACAA AGAACTTCAA GAAACAAGTC AGCAGTTTTT ATCAGCAACA GCCCAAGCCA GAATTGCTCA AGCTGAAAAA CAAGCGCAAG AACTGTTAGC ATTTTATCAA GAAGTTCGGG AAACAAGTCA GCAGTTTTTA TCAGCAACAG CCCAAGCAAG AATTGCTCAA GCTGAAAAAC AAGCTCAAGA ACTGTTAGCA TTCCACAAAG AACTTCAAGA AACAAGTCAG CAGTTTTTAT CAGCAACAGC CGACGCAAGA ACTGCTCAAG CTAAGGAACA GAAGGAATCT CTCCTGAAAT TCCGTCAGGA TTTGTTTGTG AGTATCTTTG GTTAATAAAG ATCTAACTAT TGGAGGCTAC TAAAAATGGA AGACGCCAAA AACATAAAGA AAGGCCCGGC GCCATTCTAT CCTCTAGAGG ATGGAACCGC TGGAGAGCAA CTGCATAAGG CTATGAAGAG ATACGCCCTG GTTCCTGGAA CAATTGCTTT TACAGATGCA CATATCGAGG TGAACATCAC GTACGCGGAA TACTTCGAAA TGTCCGTTCG GTTGGCAGAA GCTATGAAAC GATATGGGCT GAATACAAAT CACAGAATCG TCGTATGCAG TGAAAACTCT CTTCAATTCT TTATGCCGGT GTTGGGCGCG TTATTTATCG GAGTTGCAGT TGCGCCCGCG AACGACATTT ATAATGAACG TGAATTGCTC AACAGTATGA ACATTTCGCA GCCTACCGTA GTGTTTGTTT CCAAAAAGGG GTTGCAAAAA ATTTTGAACG TGCAAAAAAA ATTACCAATA ATCCAGAAAA TTATTATCAT GGATTCTAAA ACGGATTACC AGGGATTTCA GTCGATGTAC ACGTTCGTCA CATCTCATCT ACCTCCCGGT TTTAATGAAT ACGATTTTGT ACCAGAGTCC TTTGATCGTG ACAAAACAAT TGCACTGATA ATGAATTCCT CTGGATCTAC TGGGTTACCT AAGGGTGTGG CCCTTCCGCA TAGAACTGCC TGCGTCAGAT TCTCGCATGC CAGAGATCCT ATTTTTGGCA ATCAAATCAT TCCGGATACT GCGATTTTAA GTGTTGTTCC ATTCCATCAC GGTTTTGGAA TGTTTACTAC ACTCGGATAT TTGATATGTG GATTTCGAGT CGTCTTAATG TATAGATTTG AAGAAGAGCT GTTTTTACGA TCCCTTCAGG ATTACAAAAT TCAAAGTGCG TTGCTAGTAC CAACCCTATT TTCATTCTTC GCCAAAAGCA CTCTGATTGA CAAATACGAT TTATCTAATT TACACGAAAT TGCTTCTGGG GGCGCACCTC TTTCGAAAGA AGTCGGGGAA GCGGTTGCAA AACGCTTCCA TCTTCCAGGG ATACGACAAG GATATGGGCT CACTGAGACT ACATCAGCTA TTCTGATTAC ACCCGAGGGG GATGATAAAC CGGGCGCGGT CGGTAAAGTT GTTCCATTTT TTGAAGCGAA GGTTGTGGAT CTGGATACCG GGAAAACGCT GGGCGTTAAT CAGAGAGGCG AATTATGTGT CAGAGGACCT ATGATTATGT CCGGTTATGT AAACAATCCG GAAGCGACCA ACGCCTTGAT TGACAAGGAT GGATGGCTAC ATTCTGGAGA CATAGCTTAC TGGGACGAAG ACGAACACTT CTTCATAGTT GACCGCTTGA AGTCTTTAAT TAAATACAAA GGATATCAGG TGGCCCCCGC TGAATTGGAA TCGATATTGT TACAACACCC CAACATCTTC GACGCGGGCG TGGCAGGTCT TCCCGACGAT GACGCCGGTG AACTTCCCGC CGCCGTTGTT GTTTTGGAGC ACGGAAAGAC GATGACGGAA AAAGAGATCG TGGATTACGT CGCCAGTCAA GTAACAACCG CGAAAAAGTT GCGCGGAGGA GTTGTGTTTG TGGACGAAGT ACCGAAAGGT CTTACCGGAA AACTCGACGC AAGAAAAATC AGAGAGATCC TCATAAAGGC CAAGAAGGGC GGAAAGTCCA AATTGGGTGG AGGCGGATCA ATGGAAATTA AAAAAATTAT GCAAGCCGTG AACGACTTTT TCGGTGAACA CGTAGCTCCT CCTCATAAAA TTACCTCGGT GGAAGCTACT GAAGATGAAG GTTGGAGAGT TATTGTTGAA GTCATTGAAG AACGAGAATA TATGAAAAAA TACGCCAAAG ATGAAATGCT CGGAACGTAC GAGTGCTTTG TAAATAAAGA AAAAGAAGTC ATTTCATTCA AACGACTCGA CGTCAGATAT AGAAGCGCCA TTGGCATTGA AGCATAAACG ATAAGATGGC AGGAGGAACG TAAAAATGAC CGTCTTAACA GACAAAAGGA AAAAAGGCAG TGGAGCTTTT ATACAAGATG ACGAGACAAA AGAGGTTCTT TCAAGAGCGC TGAGCTATTT AAAATCCGGC TATTCCATTC ATTTTACAGG TCCTGCCGGC GGAGGCAAAA CCTCTTTAGC GCGAGCGCTT GCTAAAAAGA GAAAGCGTCC TGTAATGCTG ATGCACGGGA ATCACGAGCT CAACAACAAA GATTTAATTG GCGATTTTAC GGGATACACG AGCAAAAAAG TAATCGACCA GTACGTTCGT TCTGTCTATA AAAAAGATGA ACAGGTGAGT GAAAACTGGC AGGATGGCCG ATTGCTTGAA GCTGTAAAAA ATGGCTATAC GCTGATTTAC GACGAATTTA CTCGTTCTAA GCCTGCGACG AATAATATCT TTCTATCGAT ATTAGAAGAA GGCGTGCTGC CGCTGTATGG AGTAAAAATG ACCGATCCTT TTGTGCGCGT GCATCCCGAT TTCCGCGTCA TCTTCACAAG CAATCCAGCT GAGTATGCCG GCGTATATGA TACGCAAGAT GCGCTTCTCG ACAGGTTAAT TACCATGTTT ATTGATTATA AAGACATCGA CAGAGAGACA GCGATTTTAA CGGAGAAAAC GGACGTAGAA GAAGATGAAG CGCGCACAAT TGTAACGCTC GTAGCAAACG TGCGAAACCG CTCTGGAGAC GAAAACAGCA GCGGACTTAG CCTGCGGGCT TCGCTTATGA TCGCTACCCT TGCCACGCAG CAAGACATTC CTATCGATGG AAGTGACGAA GATTTTCAAA CGTTATGTAT CGATATTTTG CATCATCCGC TTACCAAATG TTTGGATGAA GAAAATGCAA AAAGCAAAGC CGAAAAAATC ATTTTAGAAG AATGTAAGAA TATAGACACT GAAGAAAAGT AAAGGAGCTT GAAAACATGA GTGAAACAAA CGAAACAGGT ATTTATATTT TTAGCGCCAT TCAAACGGAT AAAGACGAAG AATTTGGCGC CGTGGAAGTA GAAGGAACAA AAGCTGAAAC ATTTTTGATT CGCTACAAAG ACGCGGCTAT GGTAGCAGCT GAAGTACCGA TGAAAATTTA TCATCCTAAT CGCCAAAATT TATTAATGCA TCAAAACGCA GTAGCAGCGA TTATGGACAA GAACGATACG GTTATTCCAA TCAGCTTTGG GAATGTATTC AAATCAAAAG AAGACGTAAA AGTTCTTTTG GAAAACCTTT ATCCGCAGTT TGAAAAGCTG TTTCCAGCGA TCAAAGGAAA AATTGAAGTC GGTTTAAAAG TAATTGGGAA AAAAGAATGG CTTGAGAAAA AAGTAAACGA AAATCCTGAA CTTGAGAAAG TATCAGCATC CGTAAAAGGA AAATCAGAAG CAGCCGGTTA TTATGAGCGT ATTCAACTTG GAGGAATGGC TCAAAAGATG TTTACTTCCC TGCAAAAAGA AGTCAAGACA GATGTGTTTT CTCCGCTTGA AGAAGCAGCG GAAGCAGCAA AAGCAAATGA GCCAACGGGC GAAACGATGC TTTTAAACGC GTCTTTCTTA ATTAACCGAG AAGATGAAGC GAAGTTTGAT GAAAAAGTAA ATGAAGCGCA TGAAAACTGG AAAGACAAAG CCGATTTTCA TTACAGCGGT CCTTGGCCTG CTTATAATTT TGTGAACATT CGCCTAAAAG TAGAAGAGAA ATAACGTGCT TCACAAATTA GTAACCGCAC CCATTAACCT TGTAGTGAAA ATCGGCGAAA AAGTACAGGA AGAAGCTGAT AAACAGCTAT ATGACCTTCC GACGATTCAG CAAAAGCTCA TTCAGCTTCA AATGATGTTT GAGCTTGGTG AAATTCCAGA AGAAGCGTTT CAAGAAAAAG AAGATGAATT GTTAATGAGG TACGAAATTG CGAAACGCAG AGAAATTGAA CAATGGGAAG AGCTAACACA AAAAAGAAAT GAGGAATCCT AGATGGGAGA ATTACTGTAT TTATACGGTT TAATTCCAAC AAAAGAAGCA GCAGCCATAG AGCCGTTTCC ATCTTATAAG GGGTTTGACG GAGAACATTC ACTGTACCCA ATTGCGTTTG ATCAGGTGAC GGCTGTAGTT TCTAAGCTGG ATGCTGACAC CTATTCAGAA AAAGTGATTC AAGAAAAAAT GGAGCAGGAT ATGAGCTGGC TGCAGGAAAA AGCATTTCAT CATCACGAAA CGGTAGCCGC TTTGTACGAA GAATTTACGA TCATTCCATT AAAATTTTGC ACCATTTATA AAGGTGAAGA AAGTCTGCAG GCAGCTATTG AGATTAACAA AGAAAAGATA GAGAATTCAC TGACGCTGCT TCAAGGAAAT GAAGAGTGGA ATGTGAAAAT TTACTGTGAT GATACAGAGC TTAAAAAAGG AATCAGCGAA ACGAATGAAA GCGTGAAAGC GAAAAAACAA GAAATTAGTC ACTTATCACC AGGAAGACAG TTTTTTGAAA AGAAAAAAAT AGATCAGCTG ATTGAAAAAG AATTAGAGCT TCACAAAAAC AAAGTGTGTG AAGAGATACA TGACAAGCTA AAAGAATTAT CGCTTTATGA CTCTGTTAAA AAGAATTGGA GCAAAGACGT AACTGGCGCA GCTGAACAGA TGGCGTGGAA CAGCGTGTTT CTTCTCCCGT CTCTGCAGAT TACTAAGTTC GTAAACGAAA TAGAAGAGCT TCAGCAAAGG CTTGAAAATA AAGGCTGGAA GTTTGAAGTG ACGGGACCAT GGCCGCCCTA TCATTTCTCG AGCTTTGCGT AAAGTGAGGA ATTAACATTA TGTCTCTTAA ACAATCCATG GAGAATAAAG ATATTGCTCT TATTGATATT TTAGATGTCA TTTTAGATAA AGGAGTCGCC ATTAAAGGAG ACTTAATCAT TTCCATAGCT GGCGTCGATT TAGTGTATTT GGATTTGCGG GTGCTTATTT CTTCGGTTGA AACGCTTGTG CAAGCAAAAG AAGGAAATCA CAAACCAATC ACTTCTGAAC AATTTGATAA ACAAAAGGAG GAATTAATGG ATGCAACCGG TCAGCCAAGC AAATGGACGA ATCCACTTGG ATCCTGATCA AGCTGAACAA GGCTTAGCGC AGCTTGTGAT GACAGTTATT GAGCTATTGA GGCAAATCGT TGAACGTCAT GCCATGAGGC GGGTGGAGGG TGGAACGTTG ACGGACGAAC AAATTGAAAA CTTAGGAATT GCACTAATGA ACTTAGAAGA AAAAATGGAC GAGTTGAAAG AGGTGTTCGG TCTGGATGCA GAAGATTTAA ATATTGATCT TGGACCGCTA GGCAGCCTGC TTTAAGCGGT CAGTAGGAGG AACAGTATGG CAGTCGAACA TAATATGCAG TCAAGTACGA TTGTAGATGT GCTCGAAAAG ATTTTGGATA AAGGAGTCGT TATAGCGGGG GACATCACCG TAGGAATTGC AGATGTCGAG CTATTAACGA TAAAGATCCG CTTGATTGTG GCTTCGGTTG ATAAGGCAAA AGAAATCGGC ATGGACTGGT GGGAAAATGA TCCGTATCTC AGTTCAAAAG GAGCCAATAA CAAAGCGCTC GAAGAAGAAA ATAAAATGCT GCATGAGCGG TTAAAAACGC TTGAAGAAAA AATAGAAACG AAACGTTAAA AACTGTACGC TACTTAAAAA ATGGAGGGAT TTACAATGGC AACTGAAACA AAATTAGATA ACACACAGGC AGAAAACAAG GAAAATAAAA ATGCGGAAAA CGGTTCAAAA GAAAAGAACG GTTCAAAAGC AAGCAAAACA ACAAGCAGCG GGCCAATCAA ACGAGCGGTA GCAGGAGGCA TCATCGGTGC AACGATTGGA TATGTATCGA CTCCTGAAAA TCGAAAAAGT CTCCTTGACC GCATTGATAC AGACGAATTA AAAAGCAAAG CATCTGATTT AGGAACAAAG GTAAAAGAAA AATCAAAAAG CAGCGTGGCC AGCCTGAAAA CATCTGCGGG AAGCTTGTTT AAAAAAGATA AAGATAAATC AAAAGATGAT GAAGAAAACG TAAATTCTTC TAGTAGCGAA ACAGAAGACG ATAACGTTCA AGAGTACGAC GAGTTAAAAG AAGAAAATCA AACTCTTCAA GATCGCTTAT CACAGCTTGA AGAAAAAATG AACATGCTTG TTGAGCTTAG CCTCAATAAA AATCAAGACG AAGAAGCGGA AGATACAGAT TCCGACGAAG AAGAGAACGA TGAGAACGAT GAAAACGATG AAAACGAGCA GGACGATGAA AACGAAGAAG AAACATCTAA GCCACGTAAA AAGGATAAAA AAGAAGCTGA GGAAGAAGAA AGTGAAAGTG ACGAAGACAG CGAGGAAGAA GAGGAAGATT CTCGCTCAAA CAAAAAAAAT AAAAAAGTAA AAACAGAAGA AGAAGACGAA GATGAAAGCG AAGAAGAAAA AAAGGAAGCG AAACCAAAAA AGTCAACAGC TAAAAAATCA AAAAATACAA AAGCAAAGAA AAACACGGAC GAAGAAGATG ATGAAGCAAC ATCTCTTTCT AGTGAAGACG ATACAACAGC CTAAGACGTA AAGGAGGAAA GAAAGACATG AGTACAGGCC CTTCTTTTTC AACTAAAGAC AATACGCTTG AATACTTTGT GAAAGCTTCT AATAAACACG GCTTTTCACT TGATATTTCA TTAAATGTAA ACGGCGCTGT GATTTCCGGT ACCATGATTT CAGCAAAAGA ATACTTTGAT TACTTAAGCG AAACGTTTGA AGAAGGCAGT GAAGTGGCTC AGGCGCTAAG CGAACAATTC TCTTTAGCAA GCGAAGCGAG CGAATCAAAC GGAGAAGCAG AAGCCCATTT TATTCATTTG AAAAATACAA AGATTTACTG TGGAGACAGT AAATCTACTC CTTCTAAAGG CAAAATCTTT TGGAGAGGGA AAATAGCAGA AGTAGACGGG TTTTTCTTAG GAAAGATTTC TGATGCAAAA TCAACGAGTA AAAAGAGTTC ATAAAAAACG GCGGGGTGAT TGCCCCGCCG TTTTTTAGTG ATGTGATGAG ATGTGCAGCT TCTTTTTTCG ATACATGAGC GGAGTCATCT TCATTTCTTT TCGAAACTGA TTAATAAAAT AGCTTGTACT ATTAAACCCT ACTTGATAGG CGACTTCCGT CACATTTGCT TCTGCTTGCT GCAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT
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