pCT74-EYFP-GUS-HygR
$55.00
In stock
SKU
PL20641
-
100μg liquid plasmid(Instantly):$76
| name | pCT74-EYFP-GUS-HygR | another name | apCT74-EYFP-GUS-HygR |
| Insert Size (bp) | Plasmid host | filiformFungus | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Amp |
| Selective marker | Hyg | Fluorescent labeling | Yellow |
| Promoter | T3 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pCT74-HygR-RFP | 5′ sequencing primer | PEGFP-C-5 |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GAGCTCCAAT TCGCCCTATA GTGAGTCGTA TTACAATTCA CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATGGGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA ACGCTTACAA TTTAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAAG CTCGAAATTA ACCCTCACTA AAGGGAACAA AAGCTGGTAC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCTTCGGCT ACGGCCTGCA GTGCTTCGCC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC TACCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGCT CGAGGGCGGT GGAGGAAGCG GCGGAGGTGG ATCTATGTTA CGTCCTGTAG AAACCCCAAC CCGTGAAATC AAAAAACTCG ACGGCCTGTG GGCATTCAGT CTGGATCGCG AAAACTGTGG AATTGATCAG CGTTGGTGGG AAAGCGCGTT ACAAGAAAGC CGGGCAATTG CTGTGCCAGG CAGTTTTAAC GATCAGTTCG CCGATGCAGA TATTCGTAAT TATGCGGGCA ACGTCTGGTA TCAGCGCGAA GTCTTTATAC CGAAAGGTTG GGCAGGCCAG CGTATCGTGC TGCGTTTCGA TGCGGTCACT CATTACGGCA AAGTGTGGGT CAATAATCAG GAAGTGATGG AGCATCAGGG CGGCTATACG CCATTTGAAG CCGATGTCAC GCCGTATGTT ATTGCCGGGA AAAGTGTACG TATCACCGTT TGTGTGAACA ACGAACTGAA CTGGCAGACT ATCCCGCCGG GAATGGTGAT TACCGACGAA AACGGCAAGA AAAAGCAGTC TTACTTCCAT GATTTCTTTA ACTATGCCGG AATCCATCGC AGCGTAATGC TCTACACCAC GCCGAACACC TGGGTGGACG ATATCACCGT GGTGACGCAT GTCGCGCAAG ACTGTAACCA CGCGTCTGTT GACTGGCAGG TGGTGGCCAA TGGTGATGTC AGCGTTGAAC TGCGTGATGC GGATCAACAG GTGGTTGCAA CTGGACAAGG CACTAGCGGG ACTTTGCAAG TGGTGAATCC GCACCTCTGG CAACCGGGTG AAGGTTATCT CTATGAACTG TGCGTCACAG CCAAAAGCCA GACAGAGTGT GATATCTACC CGCTTCGCGT CGGCATCCGG TCAGTGGCAG TGAAGGGCGA ACAGTTCCTG ATTAACCACA AACCGTTCTA CTTTACTGGC TTTGGTCGTC ATGAAGATGC GGACTTGCGT GGCAAAGGAT TCGATAACGT GCTGATGGTG CACGACCACG CATTAATGGA CTGGATTGGG GCCAACTCCT ACCGTACCTC GCATTACCCT TACGCTGAAG AGATGCTCGA CTGGGCAGAT GAACATGGCA TCGTGGTGAT TGATGAAACT GCTGCTGTCG GCTTTAACCT CTCTTTAGGC ATTGGTTTCG AAGCGGGCAA CAAGCCGAAA GAACTGTACA GCGAAGAGGC AGTCAACGGG GAAACTCAGC AAGCGCACTT ACAGGCGATT AAAGAGCTGA TAGCGCGTGA CAAAAACCAC CCAAGCGTGG TGATGTGGAG TATTGCCAAC GAACCGGATA CCCGTCCGCA AGGTGCACGG GAATATTTCG CGCCACTGGC GGAAGCAACG CGTAAACTCG ACCCGACGCG TCCGATCACC TGCGTCAATG TAATGTTCTG CGACGCTCAC ACCGATACCA TCAGCGATCT CTTTGATGTG CTGTGCCTGA ACCGTTATTA CGGATGGTAT GTCCAAAGCG GCGATTTGGA AACGGCAGAG AAGGTACTGG AAAAAGAACT TCTGGCCTGG CAGGAGAAAC TGCATCAGCC GATTATCATC ACCGAATACG GCGTGGATAC GTTAGCCGGG CTGCACTCAA TGTACACCGA CATGTGGAGT GAAGAGTATC AGTGTGCATG GCTGGATATG TATCACCGCG TCTTTGATCG CGTCAGCGCC GTCGTCGGTG AACAGGTATG GAATTTCGCC GATTTTGCGA CCTCGCAAGG CATATTGCGC GTTGGCGGTA ACAAGAAAGG GATCTTCACT CGCGACCGCA AACCGAAGTC GGCGGCTTTT CTGCTGCAAA AACGCTGGAC TGGCATGAAC TTCGGTGAAA AACCGCAGCA GGGAGGCAAA CAATGACTCG AGGTCGACGT TAACTGGTTC CCGGTCGGCA TCTACTCTAT TCCTTTGCCC TCGGACGAGT GCTGGGGCGT CGGTTTCCAC TATCGGCGAG TACTTCTACA CAGCCATCGG TCCAGACGGC CGCGCTTCTG CGGGCGATTT GTGTACGCCC GACAGTCCCG GCTCCGGATC GGACGATTGC GTCGCATCGA CCCTGCGCCC AAGCTGCATC ATCGAAATTG CCGTCAACCA AGCTCTGATA GAGTTGGTCA AGACCAATGC GGAGCATATA CGCCCGGAGG CGCGGCGATC CTGCAAGCTC CGGATGCCTC CGCTCGAAGT AGCGCGTCTG CTGCTCCATA CAAGCCAACC ACGGCCTCCA GAAGAGGATG TTGGCGACCT CGTATTGGGA ATCCCCGAAC ATCGCCTCGC TCCAGTCAAT GACCGCTGTT ATGCGGCCAT TGTCCGTCAG GACATTGTTG GAGCCGAAAT CCGCATGCAC GAGGTGCCGG ACTTCGGGGC AGTCCTCGGC CCAAAGCATC AGCTCATCGA GAGCCTGCGC GACGGACGCA CTGACGGTGT CGTCCATCAC AGTTTGCCAG TGATACACAT GGGGATCAGC AATCGCGCAT ATGAAATCAC GCCATGTAGT GTATTGACCG ATTCCTTGCG GTCCGAATGG GCCGAACCCG CTCGTCTGGC TAAGATCGGC CGCAGCGATC GCATCCATGG CCTCCGCGAC CGGCTGGAGA ACAGCGGGCA GTTCGGTTTC AGGCAGGTCT TGCAACGTGA CACCCTGTGC ACGGCGGGAG ATGCAATAGG TCAGGCTCTC GCTGAACTCC CCAATGTCAA GCACTTCCGG AATCGGGAGC GCGGCCGATG CAAAGTGCCG ATAAACATAA CGATCTTTGT AGAAACCATC GGCGCAGCTA TTTACCCGCA GGACATATCC ACGCCCTCCT ACATCGAAGC TGAAAGCACG AGATTCTTCG CCCTCCGAGA GCTGCATCAG GTCGGAGACG CTGTCGAACT TTTCGATCAG AAACTTCTCG ACAGACGTCG CGGTGAGTTC AGGCTTTTTC ATTTGGATGC TTGGGTAGAA TAGGTAAGTC AGATTGAATC TGAAATAAAG GGAGGAAGGG CGAACTTAAG AAGGTATGAC CGGGTCGTCC ACTTACCTTG CTTGACAAAC GCACCAAGTT ATCGTGCACC AAGCAGCAGA TGATAATAAT GTCCTCGTTC CTGTCTGCTA ATAAGAGTCA CACTTCGAGC GCCGCCGCTA CTGCTACAAG TGGGGCTGAT CTGACCAGTT GCCTAAATGA ACCATCTTGT CAAACGACAC AAATTTTGTG CTCACCGCCT GGACGACTAA ACCAAAATAG GCATTCATTG TTGACCTCCA CTAGCTCCAG CCAAGCCCAA AAAATGCTCC TTCAATATCA GTTAACGTCG ACGGTATCGA TGAATTCCTG CAGCCCGGGG GATCCACTAG TTCTAGAGCG GCCGCCACCG CGGTG
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