pLV-TRE-PspCas13b-NES-ALKBH5-EGFP-EF1a-BSD
$113.00
In stock
SKU
PL20602
-
100μg liquid plasmid(Instantly):$134
| name | pLV-TRE-PspCas13b-NES-ALKBH5-EGFP-EF1a-BSD | another name | apLV-TRE-PspCas13b-NES-ALKBH5-EGFP-EF1a-BSD |
| Insert Size (bp) | 4524bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression,Tetracycline induction | Fragment type | |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Blast | Fluorescent labeling | Green |
| Promoter | tight TRE | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | PLV-TRE-EGFP-EFS-NS-Bla | 5′ sequencing primer | TRE3GV-F2 |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGCACGGAGC TACATCTGGC CCAGTGCTGC ATGATACCGC GTGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCGAC GGATCGGGAG ATCTCCCGAT CCCCTATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GCGCGTTTTG CCTGTACTGG GTGTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG TGGCGCCCGA ACAGGGACTT GAAAGCGAAA GGGAAACCAG AGGAGCTCTC TCGACGCAGG ACTCGGCTTG CTGAAGCGCG CACGGCAAGA GGCGAGGGGC GGCGACTGGT GAGTACGCCA AAAATTTTGA CTAGCGGAGG CTAGAAGGAG AGAGATGGGT GCGAGAGCGT CAGTATTAAG CGGGGGAGAA TTAGATCGCG ATGGGAAAAA ATTCGGTTAA GGCCAGGGGG AAAGAAAAAA TATAAATTAA AACATATAGT ATGGGCAAGC AGGGAGCTAG AACGATTCGC AGTTAATCCT GGCCTGTTAG AAACATCAGA AGGCTGTAGA CAAATACTGG GACAGCTACA ACCATCCCTT CAGACAGGAT CAGAAGAACT TAGATCATTA TATAATACAG TAGCAACCCT CTATTGTGTG CATCAAAGGA TAGAGATAAA AGACACCAAG GAAGCTTTAG ACAAGATAGA GGAAGAGCAA AACAAAAGTA AGACCACCGC ACAGCAAGCG GCCGCTGATC TTCAGACCTG GAGGAGGAGA TATGAGGGAC AATTGGAGAA GTGAATTATA TAAATATAAA GTAGTAAAAA TTGAACCATT AGGAGTAGCA CCCACCAAGG CAAAGAGAAG AGTGGTGCAG AGAGAAAAAA GAGCAGTGGG AATAGGAGCT TTGTTCCTTG GGTTCTTGGG AGCAGCAGGA AGCACTATGG GCGCAGCGTC AATGACGCTG ACGGTACAGG CCAGACAATT ATTGTCTGGT ATAGTGCAGC AGCAGAACAA TTTGCTGAGG GCTATTGAGG CGCAACAGCA TCTGTTGCAA CTCACAGTCT GGGGCATCAA GCAGCTCCAG GCAAGAATCC TGGCTGTGGA AAGATACCTA AAGGATCAAC AGCTCCTGGG GATTTGGGGT TGCTCTGGAA AACTCATTTG CACCACTGCT GTGCCTTGGA ATGCTAGTTG GAGTAATAAA TCTCTGGAAC AGATTTGGAA TCACACGACC TGGATGGAGT GGGACAGAGA AATTAACAAT TACACAAGCT TAATACACTC CTTAATTGAA GAATCGCAAA ACCAGCAAGA AAAGAATGAA CAAGAATTAT TGGAATTAGA TAAATGGGCA AGTTTGTGGA ATTGGTTTAA CATAACAAAT TGGCTGTGGT ATATAAAATT ATTCATAATG ATAGTAGGAG GCTTGGTAGG TTTAAGAATA GTTTTTGCTG TACTTTCTAT AGTGAATAGA GTTAGGCAGG GATATTCACC ATTATCGTTT CAGACCCACC TCCCAACCCC GAGGGGACCC GACAGGCCCG AAGGAATAGA AGAAGAAGGT GGAGAGAGAG ACAGAGACAG ATCCATTCGA TTAGTGAACG GATCGGCACT GCGTGCGCCA ATTCTGCAGA CAAATGGCAG TATTCATCCA CAATTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT TCGGGTTTAT TACAGGGACA GCAGAGATCC AGTTTGGTTA ATTAACAACT TTGTATAGAA AAGTTGGCTC CGAATTTCTC GAGGAATTCC AATTGACCAT GACCGGTTTG TCGAGTTTAC TCCCTATCAG TGATAGAGAA CGTATGTCGA GTTTACTCCC TATCAGTGAT AGAGAACGAT GTCGAGTTTA CTCCCTATCA GTGATAGAGA ACGTATGTCG AGTTTACTCC CTATCAGTGA TAGAGAACGT ATGTCGAGTT TACTCCCTAT CAGTGATAGA GAACGTATGT CGAGTTTATC CCTATCAGTG ATAGAGAACG TATGTCGAGT TTACTCCCTA TCAGTGATAG AGAACGTATG TCGAGGTAGG CGTGTACGGT GGGAGGCCTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA GATCGCCTGG AGATTTCGAG CTCGGTACCC GGGTAACTGA TCATAATTCG ACCCAAGTTT GTACAAAAAA GCAGGCTGAT TACCGGTCGC CACCGCCACC ATGAACATCC CCGCTCTGGT GGAAAACCAG AAGAAGTACT TTGGCACCTA CAGCGTGATG GCCATGCTGA ACGCTCAGAC CGTGCTGGAC CACATCCAGA AGGTGGCCGA TATTGAGGGC GAGCAGAACG AGAACAACGA GAATCTGTGG TTTCACCCCG TGATGAGCCA CCTGTACAAC GCCAAGAACG GCTACGACAA GCAGCCCGAG AAAACCATGT TCATCATCGA GCGGCTGCAG AGCTACTTCC CATTCCTGAA GATCATGGCC GAGAACCAGA GAGAGTACAG CAACGGCAAG TACAAGCAGA ACCGCGTGGA AGTGAACAGC AACGACATCT TCGAGGTGCT GAAGCGCGCC TTCGGCGTGC TGAAGATGTA CAGGGACCTG ACCAACGCAT ACAAGACCTA CGAGGAAAAG CTGAACGACG GCTGCGAGTT CCTGACCAGC ACAGAGCAAC CTCTGAGCGG CATGATCAAC AACTACTACA CAGTGGCCCT GCGGAACATG AACGAGAGAT ACGGCTACAA GACAGAGGAC CTGGCCTTCA TCCAGGACAA GCGGTTCAAG TTCGTGAAGG ACGCCTACGG CAAGAAAAAG TCCCAAGTGA ATACCGGATT CTTCCTGAGC CTGCAGGACT ACAACGGCGA CACACAGAAG AAGCTGCACC TGAGCGGAGT GGGAATCGCC CTGCTGATCT GCCTGTTCCT GGACAAGCAG TACATCAACA TCTTTCTGAG CAGGCTGCCC ATCTTCTCCA GCTACAATGC CCAGAGCGAG GAACGGCGGA TCATCATCAG ATCCTTCGGC ATCAACAGCA TCAAGCTGCC CAAGGACCGG ATCCACAGCG AGAAGTCCAA CAAGAGCGTG GCCATGGATA TGCTCAACGA AGTGAAGCGG TGCCCCGACG AGCTGTTCAC AACACTGTCT GCCGAGAAGC AGTCCCGGTT CAGAATCATC AGCGACGACC ACAATGAAGT GCTGATGAAG CGGAGCAGCG ACAGATTCGT GCCTCTGCTG CTGCAGTATA TCGATTACGG CAAGCTGTTC GACCACATCA GGTTCCACGT GAACATGGGC AAGCTGAGAT ACCTGCTGAA GGCCGACAAG ACCTGCATCG ACGGCCAGAC CAGAGTCAGA GTGATCGAGC AGCCCCTGAA CGGCTTCGGC AGACTGGAAG AGGCCGAGAC AATGCGGAAG CAAGAGAACG GCACCTTCGG CAACAGCGGC ATCCGGATCA GAGACTTCGA GAACATGAAG CGGGACGACG CCAATCCTGC CAACTATCCC TACATCGTGG ACACCTACAC ACACTACATC CTGGAAAACA ACAAGGTCGA GATGTTTATC AACGACAAAG AGGACAGCGC CCCACTGCTG CCCGTGATCG AGGATGATAG ATACGTGGTC AAGACAATCC CCAGCTGCCG GATGAGCACC CTGGAAATTC CAGCCATGGC CTTCCACATG TTTCTGTTCG GCAGCAAGAA AACCGAGAAG CTGATCGTGG ACGTGCACAA CCGGTACAAG AGACTGTTCC AGGCCATGCA GAAAGAAGAA GTGACCGCCG AGAATATCGC CAGCTTCGGA ATCGCCGAGA GCGACCTGCC TCAGAAGATC CTGGATCTGA TCAGCGGCAA TGCCCACGGC AAGGATGTGG ACGCCTTCAT CAGACTGACC GTGGACGACA TGCTGACCGA CACCGAGCGG AGAATCAAGA GATTCAAGGA CGACCGGAAG TCCATTCGGA GCGCCGACAA CAAGATGGGA AAGAGAGGCT TCAAGCAGAT CTCCACAGGC AAGCTGGCCG ACTTCCTGGC CAAGGACATC GTGCTGTTTC AGCCCAGCGT GAACGATGGC GAGAACAAGA TCACCGGCCT GAACTACCGG ATCATGCAGA GCGCCATTGC CGTGTACGAT AGCGGCGACG ATTACGAGGC CAAGCAGCAG TTCAAGCTGA TGTTCGAGAA GGCCCGGCTG ATCGGCAAGG GCACAACAGA GCCTCATCCA TTTCTGTACA AGGTGTTCGC CCGCAGCATC CCCGCCAATG CCGTCGAGTT CTACGAGCGC TACCTGATCG AGCGGAAGTT CTACCTGACC GGCCTGTCCA ACGAGATCAA GAAAGGCAAC AGAGTGGATG TGCCCTTCAT CCGGCGGGAC CAGAACAAGT GGAAAACACC CGCCATGAAG ACCCTGGGCA GAATCTACAG CGAGGATCTG CCCGTGGAAC TGCCCAGACA GATGTTCGAC AATGAGATCA AGTCCCACCT GAAGTCCCTG CCACAGATGG AAGGCATCGA CTTCAACAAT GCCAACGTGA CCTATCTGAT CGCCGAGTAC ATGAAGAGAG TGCTGGACGA CGACTTCCAG ACCTTCTACC AGTGGAACCG CAACTACCGG TACATGGACA TGCTTAAGGG CGAGTACGAC AGAAAGGGCT CCCTGCAGCA CTGCTTCACC AGCGTGGAAG AGAGAGAAGG CCTCTGGAAA GAGCGGGCCT CCAGAACAGA GCGGTACAGA AAGCAGGCCA GCAACAAGAT CCGCAGCAAC CGGCAGATGA GAAACGCCAG CAGCGAAGAG ATCGAGACAA TCCTGGATAA GCGGCTGAGC AACAGCCGGA ACGAGTACCA GAAAAGCGAG AAAGTGATCC GGCGCTACAG AGTGCAGGAT GCCCTGCTGT TTCTGCTGGC CAAAAAGACC CTGACCGAAC TGGCCGATTT CGACGGCGAG AGGTTCAAAC TGAAAGAAAT CATGCCCGAC GCCGAGAAGG GAATCCTGAG CGAGATCATG CCCATGAGCT TCACCTTCGA GAAAGGCGGC AAGAAGTACA CCATCACCAG CGAGGGCATG AAGCTGAAGA ACTACGGCGA CTTCTTTGTG CTGGCTAGCG ACAAGAGGAT CGGCAACCTG CTGGAACTCG TGGGCAGCGA CATCGTGTCC AAAGAGGATA TCATGGAAGA GTTCAACAAA TACGACCAGT GCAGGCCCGA GATCAGCTCC ATCGTGTTCA ACCTGGAAAA GTGGGCCTTC GACACATACC CCGAGCTGTC TGCCAGAGTG GACCGGGAAG AGAAGGTGGA CTTCAAGAGC ATCCTGAAAA TCCTGCTGAA CAACAAGAAC ATCAACAAAG AGCAGAGCGA CATCCTGCGG AAGATCCGGA ACGCCTTCGA TGCAAACAAT TACCCCGACA AAGGCGTGGT GGAAATCAAG GCCCTGCCTG AGATCGCCAT GAGCATCAAG AAGGCCTTTG GGGAGTACGC CATCATGAAG GGAAGCCTGC AGCTGCCTCC ACTTGAAAGA CTGACACTGG GATCCGGAGG AGGTGGAAGC GGTACCATGG CGGCCGCCAG CGGCTACACG GACCTGCGTG AGAAGCTCAA GTCCATGACG TCCCGGGACA ACTATAAGGC GGGCAGCCGG GAGGCCGCCG CCGCTGCCGC AGCCGCCGTA GCCGCCGCAG CCGCAGCCGC CGCTGCCGCC GAACCTTACC CTGTGTCCGG GGCCAAGCGC AAGTATCAGG AGGACTCGGA CCCCGAGCGC AGCGACTATG AGGAGCAGCA GCTGCAGAAG GAGGAGGAGG CGCGCAAGGT GAAGAGCGGC ATCCGCCAGA TGCGCCTCTT CAGCCAGGAC GAGTGCGCCA AGATCGAGGC CCGCATTGAC GAGGTGGTGT CCCGCGCTGA GAAGGGCCTG TACAACGAGC ACACGGTGGA CCGGGCCCCA CTGCGCAACA AGTACTTCTT CGGCGAAGGC TACACTTACG GCGCCCAGCT GCAGAAGCGC GGGCCCGGCC AGGAGCGCCT CTACCCGCCG GGCGACGTGG ACGAGATCCC CGAGTGGGTG CACCAGCTGG TGATCCAAAA GCTGGTGGAG CACCGCGTCA TCCCCGAGGG CTTCGTCAAC AGCGCCGTCA TCAACGACTA CCAGCCCGGC GGCTGCATCG TGTCTCACGT GGACCCCATC CACATCTTCG AGCGCCCCAT CGTGTCCGTG TCCTTCTTTA GCGACTCTGC GCTGTGCTTC GGCTGCAAGT TCCAGTTCAA GCCTATTCGG GTGTCGGAAC CAGTGCTTTC CCTGCCGGTG CGCAGGGGAA GCGTGACTGT GCTCAGTGGA TATGCTGCTG ATGAAATCAC TCACTGCATA CGGCCTCAGG ACATCAAGGA GCGCCGAGCA GTCATCATCC TCAGGAAGAC AAGATTAGAT GCACCCCGGT TGGAAACAAA GTCCCTGAGC AGCTCCGTGT TACCACCCAG CTATGCTTCA GATCGCCTGT CAGGAAACAA CAGGGACCCT GCTCTGAAAC CCAAGCGGTC CCACCGCAAG GCAGACCCTG ATGCTGCCCA CAGGCCACGG ATCCTGGAGA TGGACAAGGA AGAGAACCGG CGCTCGGTGC TGCTGCCCAC ACACCGGCGG AGGGGTAGCT TCAGCTCTGA GAACTACTGG CGCAAGTCAT ACGAGTCCTC AGAGGACTGC TCTGAGGCAG CAGGCAGCCC TGCCCGAAAG GTGAAGATGC GGCGGCACGG TGGAGGCGGA TCAGGAGGCG GTGGATCTAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTAAGC GGATCCACCA ATCTAGATAG GTCTTGAAAG GAGTGGGAAT TGGCTCCGGT GCCCGTCAGT GGGCAGAGCG CACATCGCCC ACAGTCCCCG AGAAGTTGGG GGGAGGGGTC GGCAATTGAT CCGGTGCCTA GAGAAGGTGG CGCGGGGTAA ACTGGGAAAG TGATGTCGTG TACTGGCTCC GCCTTTTTCC CGAGGGTGGG GGAGAACCGT ATATAAGTGC AGTAGTCGCC GTGAACGTTC TTTTTCGCAA CGGGTTTGCC GCCAGAACAC AGGACCGGTT CTAGTGCCAC CATGGCCAAG CCTTTGTCTC AAGAAGAATC CACCCTCATT GAAAGAGCAA CGGCTACAAT CAACAGCATC CCCATCTCTG AAGACTACAG CGTCGCCAGC GCAGCTCTCT CTAGCGACGG CCGCATCTTC ACTGGTGTCA ATGTATATCA TTTTACTGGG GGACCTTGTG CAGAACTCGT GGTGCTGGGC ACTGCTGCTG CTGCGGCAGC TGGCAACCTG ACTTGTATCG TCGCGATCGG AAATGAGAAC AGGGGCATCT TGAGCCCCTG CGGACGGTGC CGACAGGTGC TTCTCGATCT GCATCCTGGG ATCAAAGCCA TAGTGAAGGA CAGTGATGGA CAGCCGACGG CAGTTGGGAT TCGTGAATTG CTGCCCTCTG GTTATGTGTG GGAGGGCTAA ATAACTGATC TACCCAGCTT TCTTGTACAA AGTGGTGAAT TCCGGCAATT CGATATCAAG CTTATCGATA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAATCATC GTCCTTTCCT TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC CTCCCCGCAT CGATACCGTC GACCTCGAGT CCTAGAAAAA CATGGAGCAA TCACAAGTAG CAATACAGCA GCTACCAATG CTGATTGTGC CTGGCTAGAA GCACAAGAGG AGGAGGAGGT GGGTTTTCCA GTCACACCTC AGTACCTTTA AGACCAATGA CTTACAAGGC AGCTGTAGAT CTTAGCCACT TTTTAAAAGA AAAGGGGGGA CTGGAAGGGC TAATTCACTC CCAACGAAGA CAAGATATCC TTGATCTGTG GATCTACCAC ACACAAGGCT ACTTCCCTGA TTGGCAGAAC TACACACCAG GGCCAGGGAT CAGATATCCA CTGACCTTTG GATGGTGCTA CAAGCTAGTA CCAGTTGAGC AAGAGAAGGT AGAAGAAGCC AATGAAGGAG AGAACACCCG CTTGTTACAC CCTGTGAGCC TGCATGGGAT GGATGACCCG GAGAGAGAAG TATTAGAGTG GAGGTTTGAC AGCCGCCTAG CATTTCATCA CATGGCCCGA GAGCTGCATC CGGACTGTAC TGGGTGTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG TCAGTGTGGA AAATCTCTAG CAGGGCCCGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AAAGAACCAG CTGGGGCTCT AGGGGGTATC CCCACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTAATTCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCTGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAGCA CGTGTTGACA ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA CAAGGTGAGG AACTAAACCA TGGCCAAGTT GACCAGTGCC GTTCCGGTGC TCACCGCGCG CGACGTCGCC GGAGCGGTCG AGTTCTGGAC CGACCGGCTC GGGTTCTCCC GGGACTTCGT GGAGGACGAC TTCGCCGGTG TGGTCCGGGA CGACGTGACC CTGTTCATCA GCGCGGTCCA GGACCAGGTG GTGCCGGACA ACACCCTGGG CCTGGGTGTG GGTGCGCGGC CTGGACGAGC TGTACGCCGA GTGGTCGGAG GTCGTGTCCA CGAACTTCCG GGACGCCTCC GGGCCGGCCA TGACCGAGAT CGGCGAGCAG CCGTGGGGGC GGGAGTTCGC CCTGCGCGAC CCGGCCGGCA ACTGCGTGCA CTTCGTGGCC GAGGAGCAGG ACTGACACGT GCTACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGTGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TGCCATGCTA CAG
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