pCAG-SLC9C1(human)-10×His-FLAG
$99.00
In stock
SKU
PL20581
NM_183061.3;NHE; NHE-10; SLC9A10; sperm-NHE;
100μg liquid plasmid(Instantly):$120
| name | pCAG-SLC9C1(human)-10×His-FLAG | another name | apCAG-SLC9C1(human)-10×His-FLAG |
| Insert Size (bp) | 3531bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CAG | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pCAG-_CFCH | 5′ sequencing primer | pcaggs-F |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CACCCCCCCC TCCCCCCGAG TTGCTGAGCA CGGCCCGGCT TCGGGTGCGG GGCTCCGTAC GGGGCGTGGC GCGGGGCTCG CCGTGCCGGG CGGGGGGTGG CGGCAGGTGG GGGTGCCGGG CGGGGCGGGG CCGCCTCGGG CCGGGGAGGG CTCGGGGGAG GGGCGCGGCG GCCCCCGGAG CGCCGGCGGC TGTCGAGGCG CGGCGAGCCG CAGCCATTGC CTTTTATGGT AATCGTGCGA GAGGGCGCAG GGACTTCCTT TGTCCCAAAT CTGTGCGGAG CCGAAATCTG GGAGGCGCCG CCGCACCCCC TCTAGCGGGC GCGGGGCGAA GCGGTGCGGC GCCGGCAGGA AGGAAATGGG CGGGGAGGGC CTTCGTGCGT CGCCGCGCCG CCGTCCCCTT CTCCCTCTCC AGCCTCGGGG CTGTCCGCGG GGGGACGGCT GCCTTCGGGG GGGACGGGGC AGGGCGGGGT TCGGCTTCTG GCGTGTGACC GGCGGCTCTA GAGCCTCTGC TAACCATGTT CATGCCTTCT TCTTTTTCCT ACAGCTCCTG GGCAACGTGC TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTATC GATCCGGAGG TACCGCCACC ATGGCTGGAA TATTTAAGGA GTTTTTTTTC AGTACTGAGG ACCTCCCTGA AGTCATTCTA ACATTGTCTT TGATCAGCTC CATTGGAGCA TTTTTGAACC GGCACTTGGA AGACTTTCCA ATTCCTGTCC CTGTGATATT ATTTTTACTT GGATGCAGTT TTGAAGTATT AAGCTTTACA TCTTCACAGG TCCAAAGATA CGCAAACGCC ATACAATGGA TGAGTCCAGA CTTATTTTTT CGTATATTTA CACCAGTAGT TTTCTTTACT ACTGCATTTG ACATGGATAC GTACATGCTT CAAAAGTTAT TTTGGCAGAT ACTTTTAATT TCAATTCCCG GCTTTTTGGT TAATTATATC TTAGTTCTTT GGCATCTGGC ATCTGTAAAT CAATTACTTT TGAAGCCTAC CCAATGGTTA TTATTTTCAG CTATCCTTGT GAGTTCAGAT CCCATGCTAA CCGCAGCTGC TATAAGAGAC CTTGGGCTTT CTAGAAGCCT CATCAGTTTA ATTAATGGAG AAAGTCTGAT GACCTCTGTT ATATCATTAA TTACATTTAC TAGTATTATG GATTTTGACC AAAGACTACA AAGTAAAAGA AACCATACCT TAGCTGAAGA GATCGTGGGT GGAATTTGTT CATATATTAT AGCAAGTTTC TTGTTTGGAA TTCTAAGTTC AAAACTGATT CAATTTTGGA TGTCAACTGT TTTTGGTGAT GATGTCAATC ATATAAGTCT CATCTTTTCA ATTCTGTATC TCATCTTTTA TATTTGTGAG TTAGTTGGAA TGTCAGGAAT ATTTACTCTG GCCATTGTGG GACTTCTTTT AAATTCTACA AGTTTTAAAG CAGCAATTGA AGAAACACTT CTTCTTGAAT TCTGGACTTT TCTATCACGT ATTGCTTTTC TCATGGTGTT TACTTTCTTT GGACTTCTAA TTCCTGCACA TACATATTTG TATATAGAAT TTGTTGATAT ATACTATTCA TTAAATATCT ACTTAACATT GATTGTTTTA AGATTTCTGA CCCTTCTTTT AATAAGCCCT GTTTTGTCTC GAGTTGGTCA TGAGTTCAGT TGGCGCTGGA TATTCATAAT GGTCTGTAGT GAAATGAAGG GGATGCCTAA TATAAACATG GCCCTTCTGC TTGCCTACTC TGATCTTTAT TTTGGATCTG ACAAAGAAAA ATCTCAAATA TTATTTCATG GAGTGTTAGT ATGCCTAATA ACCCTTGTTG TCAATAGATT TATTTTGCCA GTGGCAGTTA CTATACTAGG TCTTCGTGAT GCCACATCAA CAAAATATAA ATCGGTTTGT TGCACATTTC AACACTTTCA AGAGCTAACC AAGTCTGCAG CCTCTGCCCT TAAATTTGAC AAAGATCTTG CTAATGCTGA TTGGAACATG ATTGAGAAAG CAATTACACT TGAAAACCCA TACATGTTGA ACGAAGAAGA AACAACAGAA CATCAGAAGG TGAAATGTCC ACACTGTAAC AAGGAAATAG ATGAGATCTT TAACACTGAA GCAATGGAGC TGGCCAACAG GCGTCTCTTG TCAGCACAAA TAGCAAGCTA CCAGAGACAA TACAGGAATG AGATTCTGTC CCAGAGTGCT GTCCAGGTGT TGGTTGGTGC AGCAGAAAGT TTTGGTGAGA AGAAGGGAAA ATGTATGAGT CTTGATACAA TAAAGAATTA TTCTGAAAGC CAAAAAACAG TTACCTTTGC TAGAAAACTA CTACTTAATT GGGTGTATAA TACCAGAAAG GAAAAAGAGG GCCCATCAAA ATACTTCTTT TTTCGTATAT GCCATACAAT AGTATTTACT GAGGAATTTG AACATGTTGG ATACCTTGTG ATATTAATGA ATATATTTCC CTTTATAATC TCTTGGATAT CCCAGTTAAA TGTAATCTAC CACAGCGAAT TAAAACACAC TAACTACTGT TTTCTTACAC TTTATATTCT AGAGGCACTA CTTAAGATAG CAGCAATGAG GAAGGACTTT TTTTCACATG CCTGGAACAT ATTCGAGTTA GCAATTACAT TAATTGGCAT CTTACATGTA ATACTTATTG AAATAGACAC CATTAAGTAT ATTTTTAATG AGACTGAAGT AATAGTCTTT ATAAAAGTTG TTCAATTTTT TCGTATACTA CGCATTTTCA AGCTCATAGC ACCAAAGTTG CTGCAAATAA TAGATAAAAG AATGAGTCAT CAGAAGACCT TTTGGTATGG AATACTAAAA GGCTATGTCC AAGGCGAAGC AGACATAATG ACCATAATTG ATCAGATTAC AAGTTCTAAA CAGATTAAAC AGATGTTATT AAAGCAAGTG ATAAGGAATA TGGAACATGC TATAAAAGAG CTAGGCTACT TAGAGTATGA TCACCCAGAA ATTGCTGTCA CTGTGAAAAC AAAGGAAGAA ATTAATGTTA TGCTCAATAT GGCTACAGAA ATTCTTAAGG CTTTTGGCTT AAAAGGAATT ATTAGTAAAA CTGAAGGTGC TGGAATTAAT AAGTTAATCA TGGCCAAAAA GAAAGAGGTG CTTGATTCTC AATCTATTAT CAGGCCTCTT ACTGTTGAAG AAGTTCTATA TCATATTCCG TGGCTAGATA AAAACAAAGA TTATATAAAC TTCATTCAGG AAAAAGCCAA AGTTGTAACA TTTGATTGTG GAAATGATAT ATTTGAAGAA GGTGATGAGC CCAAAGGAAT CTATATCATT ATTTCAGGCA TGGTAAAGCT TGAAAAATCA AAGCCAGGTT TAGGGATTGA TCAAATGGTG GAGTCAAAGG AGAAAGATTT TCCGATAATT GACACAGACT ATATGCTCAG TGGAGAAATA ATAGGAGAGA TAAACTGCTT AACTAATGAA CCTATGAAAT ATTCTGCCAC CTGCAAAACT GTAGTGGAGA CATGTTTTAT TCCCAAAACT CACTTGTATG ATGCTTTTGA GCAATGCTCT CCTCTCATTA AACAAAAAAT GTGGCTAAAA CTTGGACTCG CTATTACAGC CAGAAAAATC AGAGAACACT TATCTTATGA GGATTGGAAC TACAATATGC AACTAAAGCT CTCTAATATT TATGTAGTAG ATATACCAAT GAGTACCAAA ACTGATATTT ATGATGAAAA TCTAATCTAT GTTATCCTCA TACATGGAGC TGTAGAAGAT TGTCTGTTAC GAAAAACTTA TAGAGCACCT TTCTTAATTC CTATAACATG CCATCAGATA CAAAGTATTG AAGATTTCAC AAAAGTAGTG ATTATTCAAA CTCCGATTAA CATGAAAACA TTCAGAAGGA ATATTAGAAA GTTTGTTCCT AAACATAAAA GTTATCTTAC ACCAGGATTA ATAGGTTCAG TTGGAACATT GGAAGAAGGC ATTCAAGAAG AAAGAAATGT TAAGGAGGAT GGAGCACACA GTGCCGCCAC TGCCAGGAGT CCCCAGCCTT GCTCCCTGCT GGGGACAAAG TTCAACTGTA AGGAGTCCCC TAGAATAAAC CTAAGGAAAG TCAGGAAAGA GCTCGAGGGC TCTGATGAGG TTGATGCTGG CTCTCATCAC CATCACCATC ACCATCACCA TCACGGCTCT GTCGAGGATT ACAAGGATGA CGATGACAAG TGATAAGTCG AGAAGCTTGC TAGCAGATCT TTTTCCCTCT GCCAAAAATT ATGGGGACAT CATGAAGCCC CTTGAGCATC TGACTTCTGG CTAATAAAGG AAATTTATTT TCATTGCAAT AGTGTGTTGG AATTTTTTGT GTCTCTCACT CGGAAGGACA TATGGGAGGG CAAATCATTT AAAACATCAG AATGAGTATT TGGTTTAGAG TTTGGCAACA TATGCCCATA TGCTGGCTGC CATGAACAAA GGTTGGCTAT AAAGAGGTCA TCAGTATATG AAACAGCCCC CTGCTGTCCA TTCCTTATTC CATAGAAAAG CCTTGACTTG AGGTTAGATT TTTTTTATAT TTTGTTTTGT GTTATTTTTT TCTTTAACAT CCCTAAAATT TTCCTTACAT GTTTTACTAG CCAGATTTTT CCTCCTCTCC TGACTACTCC CAGTCATAGC TGTCCCTCTT CTCTTATGGA GATCCCTCGA CCTGCAGCCC AAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCGGATC TGTAACAACA CCATTTAAAT GGAGTGGTTA CAAATGGAGT GGTTAATTAA GACATGTGAG CAAAAGGCCA GCAAAAGGAT CTGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTAGATCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AAGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCGAC GGATCGGGAG ATCCCTTAAT TAACAACACC ATTTGCTCGA CATTGATTAT TGACTAGTTA TTAATAGTAA TCAATTACGG GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGT CGAGGTGAGC CCCACGTTCT GCTTCACTCT CCCCATCTCC CCCCCCTCCC CACCCCCAAT TTTGTATTTA TTTATTTTTT AATTATTTTG TGCAGCGATG GGGCGGGGGG GGGGGGGGGG GGCCCCCCCC CAGGCGGGGC GGGGCCGGGG CAAAGGGCCG GGCCGGGCCA AGGCGAAAAG GTGCGGCGGC AGCCAATCAA AGCGGCGCGC TCCGAAGTTC CT
Reviews
Write Your Own Review