pACHE(human)-Fluc-SV40-hRluc-Neo
$55.00
In stock
SKU
PL20415
-
100μg liquid plasmid(Instantly):$76
| name | pACHE(human)-Fluc-SV40-hRluc-Neo | another name | apACHE(human)-Fluc-SV40-hRluc-Neo |
| Insert Size (bp) | 719bp | Plasmid host | Mammary cell |
| Plasmid uses | Promoter detection | Fragment type | Promoter |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | Rluc,Fluc |
| Promoter | ACHE | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pMCS-Fluc-SV40-hRluc-Neo | 5′ sequencing primer | cer region-F1 |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CATGCAAGCT GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCGGCCGCT TCGAGCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA GATGTGGGAG GTTTTTTTAA GCAAGTAAAA CCTCTACAAA TGTGGTAAAA TCGAATTTTA ACAAAATATT AACGCTTACA ATTTCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATACGCG GATCTGCGCA GCACCATGGC CTGAAATAAC CTCTGAAAGA GGAACTTGGT TAGGTACCTT CTGAGGCGGA AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTTGATTCT TCTGACACAA CAGTCTCGAA CCAAAGGCTG GAGCCACCAT GGCTTCCAAG GTGTACGACC CCGAGCAACG CAAACGCATG ATCACTGGGC CTCAGTGGTG GGCTCGCTGC AAGCAAATGA ACGTGCTGGA CTCCTTCATC AACTACTATG ATTCCGAGAA GCACGCCGAG AACGCCGTGA TTTTTCTGCA TGGTAACGCT GCCTCCAGCT ACCTGTGGAG GCACGTCGTG CCTCACATCG AGCCCGTGGC TAGATGCATC ATCCCTGATC TGATCGGAAT GGGTAAGTCC GGCAAGAGCG GGAATGGCTC ATATCGCCTC CTGGATCACT ACAAGTACCT CACCGCTTGG TTCGAGCTGC TGAACCTTCC AAAGAAAATC ATCTTTGTGG GCCACGACTG GGGGGCTTGT CTGGCCTTTC ACTACTCCTA CGAGCACCAA GACAAGATCA AGGCCATCGT CCATGCTGAG AGTGTCGTGG ACGTGATCGA GTCCTGGGAC GAGTGGCCTG ACATCGAGGA GGATATCGCC CTGATCAAGA GCGAAGAGGG CGAGAAAATG GTGCTTGAGA ATAACTTCTT CGTCGAGACC ATGCTCCCAA GCAAGATCAT GCGGAAACTG GAGCCTGAGG AGTTCGCTGC CTACCTGGAG CCATTCAAGG AGAAGGGCGA GGTTAGACGG CCTACCCTCT CCTGGCCTCG CGAGATCCCT CTCGTTAAGG GAGGCAAGCC CGACGTCGTC CAGATTGTCC GCAACTACAA CGCCTACCTT CGGGCCAGCG ACGATCTGCC TAAGATGTTC ATCGAGTCCG ACCCTGGGTT CTTTTCCAAC GCTATTGTCG AGGGAGCTAA GAAGTTCCCT AACACCGAGT TCGTGAAGGT GAAGGGCCTC CACTTCAGCC AGGAGGACGC TCCAGATGAA ATGGGTAAGT ACATCAAGAG CTTCGTGGAG CGCGTGCTGA AGAACGAGCA GACCGGTGGT GGGAGCGGAG GTGGCGGATC AGGTGGCGGA GGCTCCGGAG GGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA TGGCCGCAAT AAAATATCTT TATTTTCATT ACATCTGTGT GTTGGTTTTT TGTGTGAATC GATAGCGATA ATCTTTGCGC TTGCGTTTTC CCTTGTCCAG ATAGCCCAGT AGCTGACATT CATCCGGGGT CAGCACCGTT TCTGCGGACT GGCTTTCTAC GTAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTC AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACTATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ATTCGAAATG ACCGACCAAG CGACGCCCAA CCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATTTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTATA GTCCGGAAAT ACAGGAACGC ACGCTGGATG GCCCTTCGCT GGGATGGTGA AACCATGAAA AATGGCAGCT TCAGTGGATT AAGTGGGGGT AATGTGGCCT GTACCCTCTG GTTGCATAGG TATTCATACG GTTAAAATTT ATCAGGCGCG ATTGCGGCAG TTTTTCGGGT GGTTTGTTGC CATTTTTACC TGTCTGCTGC CGTGATCGCG CTGAACGCGT TTTAGCGGTG CGTACAATTA AGGGATTATG GTAAATCCAC TTACTGTCTG CCCTCGTAGC CATCGAGATA AACCGCAGTA CTCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GAGCTCAAGC TTCGAATTCT GTTAAAGCCA TTATTTGGTT TTTGGTCTCA TACAGCTGAA TTGAATATTA ACCAATAAAT AGAGGAAATA AGAATCTTTT GTGTTAGAAG ATGTGGATCA AGGTCTGTAA TAGAAGTGGG GTTCCCTGCC AGGCATGGCA GCTCATGCCT GTAATCCCAA CACTTTGGGA GGCTGAGGCA GGAGGATCAC CTGAGGTCAG GAGTTCGAGA CCAGCCTGGC CAACATGGTG AAACCCCACC TCTGCTAAAT ATACAAAAAT TAGCCAGGTG TGGTGGCAGG CGCCTCTAGT CCCAGCTACT CAGGAGGCTG GGAAAGGAGA ATCCTTGAGC CCAGGAGGCA GAGGTTGCAG CGAGCTGAGA TGGCATCATC TCTACACTCT AGCCTGGGCG ACAGAGCGAG ACTCTGTCTA AAAAAAAAAA ATGGCGCTTC CTTCCAAGGT GGGAGACAGA GATAAAAGAG GGGTAGGGAA ATTCTCTGAA TTGGCAGAAC TGGTAGTAGA AGCAAGGCTC ATTCCTGGAT AAACAGAAAT GGTGAGAGAA GAAATGGGAA AAATCAAGGA CATCCCTGCA GTGACAGCTG TGGAGGGTGG GGAGTTTACT ATGCTAGACT ATGTAGGGAA ACGCCTCCTG GCTTGACAGG TTAGGTGCCT GTGTTGACAA CGGTTGCTGA GAGCTCAAGG TGAAAAGAGG GATAGTTCCA GGAAATGATA ACCCTGGTGG ATCCCACCAT GGAAGATGCC AAAAACATTA AGAAGGGCCC AGCGCCATTC TACCCACTCG AAGACGGGAC CGCCGGCGAG CAGCTGCACA AAGCCATGAA GCGCTACGCC CTGGTGCCCG GCACCATCGC CTTTACCGAC GCACATATCG AGGTGGACAT TACCTACGCC GAGTACTTCG AGATGAGCGT TCGGCTGGCA GAAGCTATGA AGCGCTATGG GCTGAATACA AACCATCGGA TCGTGGTGTG CAGCGAGAAT AGCTTGCAGT TCTTCATGCC CGTGTTGGGT GCCCTGTTCA TCGGTGTGGC TGTGGCCCCA GCTAACGACA TCTACAACGA GCGCGAGCTG CTGAACAGCA TGGGCATCAG CCAGCCCACC GTCGTATTCG TGAGCAAGAA AGGGCTGCAA AAGATCCTCA ACGTGCAAAA GAAGCTACCG ATCATACAAA AGATCATCAT CATGGATAGC AAGACCGACT ACCAGGGCTT CCAAAGCATG TACACCTTCG TGACTTCCCA TTTGCCACCC GGCTTCAACG AGTACGACTT CGTGCCCGAG AGCTTCGACC GGGACAAAAC CATCGCCCTG ATCATGAACA GTAGTGGCAG TACCGGATTG CCCAAGGGCG TAGCCCTACC GCACCGCACC GCTTGTGTCC GATTCAGTCA TGCCCGCGAC CCCATCTTCG GCAACCAGAT CATCCCCGAC ACCGCTATCC TCAGCGTGGT GCCATTTCAC CACGGCTTCG GCATGTTCAC CACGCTGGGC TACTTGATCT GCGGCTTTCG GGTCGTGCTC ATGTACCGCT TCGAGGAGGA GCTATTCTTG CGCAGCTTGC AAGACTATAA GATTCAATCT GCCCTGCTGG TGCCCACACT ATTTAGCTTC TTCGCTAAGA GCACTCTCAT CGACAAGTAC GACCTAAGCA ACTTGCACGA GATCGCCAGC GGCGGGGCGC CGCTCAGCAA GGAGGTAGGT GAGGCCGTGG CCAAACGCTT CCACCTACCA GGCATCCGCC AGGGCTACGG CCTGACAGAA ACAACCAGCG CCATTCTGAT CACCCCCGAA GGGGACGACA AGCCTGGCGC AGTAGGCAAG GTGGTGCCCT TCTTCGAGGC TAAGGTGGTG GACTTGGACA CCGGTAAGAC ACTGGGTGTG AACCAGCGCG GCGAGCTGTG CGTCCGTGGC CCCATGATCA TGAGCGGCTA CGTTAACAAC CCCGAGGCTA CAAACGCTCT CATCGACAAG GACGGCTGGC TGCACAGCGG CGACATCGCC TACTGGGACG AGGACGAGCA CTTCTTCATC GTGGACCGGC TGAAGAGCCT GATCAAATAC AAGGGCTACC AGGTAGCCCC AGCCGAACTG GAGAGCATCC TGCTGCAACA CCCCAACATC TTCGACGCCG GGGTCGCCGG CCTGCCCGAC GACGATGCCG GCGAGCTGCC CGCCGCAGTC GTCGTGCTGG AACACGGTAA AACCATGACC GAGAAGGAGA TCGTGGACTA TGTGGCCAGC CAGGTTACAA CCGCCAAGAA GCTGCGCGGT GGTGTTGTGT TCGTGGACGA GGTGCCTAAA GGACTGACCG GCAAGTTGGA CGCCCGCAAG ATCCGCGAGA TTCTCATTAA GGCCAAGAAG GGCGGCAAGA TCGCCGTGTA ATTCTAGTTG TTTAAACGAG CTCGCTAGCC TCGAGTCTAG AGTCGACCTG CAGG
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