pHSE401-AtU6-26-FPA-sgRNA1-AtU6-1-BRI1-sgRNA1-35S-Cas9-35S-DsRed2-NOS-Bar
$55.00
In stock
SKU
PL20323
-
100μg liquid plasmid(Instantly):$76
| name | pHSE401-AtU6-26-FPA-sgRNA1-AtU6-1-BRI1-sgRNA1-35S-Cas9-35S-DsRed2-NOS-Bar | another name | apHSE401-AtU6-26-FPA-sgRNA1-AtU6-1-BRI1-sgRNA1-35S-Cas9-35S-DsRed2-NOS-Bar |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Arabidopsis | Prokaryotic resistance | Kan |
| Selective marker | Hyg | Fluorescent labeling | |
| Promoter | AtU6,35S | Replicon | pVS1 |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | pHSE401-AtU6-26-FPA-sgRNA1-AtU6-1-BRI1-sgRNA1-35S-Cas9-35S-DsRed2-NOS-Bar | 5′ sequencing primer | G51779-F1 |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAAACGCTCT TTTCTCTTAG GTTTACCCGC CAATATATCC TGTCAAACAC TGATAGTTTA AACTGAAGGC GGGAAACGAC AATCTGATCC AAGCTCAAGC TGCTCTAGCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGCCGGT ACCTCGACTT GCCTTCCGCA CAATACATCA TTTCTTCTTA GCTTTTTTTC TTCTTCTTCG TTCATACAGT TTTTTTTTGT TTATCAGCTT ACATTTTCTT GAACCGTAGC TTTCGTTTTC TTCTTTTTAA CTTTCCATTC GGAGTTTTTG TATCTTGTTT CATAGTTTGT CCCAGGATTA GAATGATTAG GCATCGAACC TTCAAGAATT TGATTGAATA AAACATCTTC ATTCTTAAGA TATGAAGATA ATCTTCAAAA GGCCCCTGGG AATCTGAAAG AAGAGAAGCA GGCCCATTTA TATGGGAAAG AACAATAGTA TTTCTTATAT AGGCCCATTT AAGTTGAAAA CAATCTTCAA AAGTCCCACA TCGCTTAGAT AAGAAAACGA AGCTGAGTTT ATATACAGCT AGAGTCGAAG TAGTGATTGC TCTTCCTCCA GGTCATCCTG TTTTAGAGCT AGAAATAGCA AGTTAAAATA AGGCTAGTCC GTTATCAACT TGAAAAAGTG GCACCGAGTC GGTGCTTTTT TTTGCAAAAT TTTCCAGATC GATTTCTTCT TCCTCTGTTC TTCGGCCATC GCTACCTTAG GACCGTTATA GTTAATTACC CTGTTATCCC TATTAATTAA GAGCTCAGAA ATCTCAAAAT TCCGGCAGAA CAATTTTGAA TCTCGATCCG TAGAAACGAG ACGGTCATTG TTTTAGTTCC ACCACGATTA TATTTGAAAT TTACGTGAGT GTGAGTGAGA CTTGCATAAG AAAATAAAAT CTTTAGTTGG GAAAAAATTC AATAATATAA ATGGGCTTGA GAAGGAAGCG AGGGATAGGC CTTTTTCTAA AATAGGCCCA TTTAAGCTAT TAACAATCTT CAAAAGTACC ACAGCGCTTA GGTAAAGAAA GCAGCTGAGT TTATATATGG TTAGAGACGA AGTAGTGATT GTTGGGTCAT AACGATATCT CGTTTTAGAG CTAGAAATAG CAAGTTAAAA TAAGGCTAGT CCGTTATCAA CTTGAAAAAG TGGCACCGAG TCGGTGCTTT TTTTCTAGAC CCAGCTTTCT TGTACAAGCT TGCATGCCTG CAGGTCAACA TGGTGGAGCA CGACACACTT GTCTACTCCA AAAATATCAA AGATACAGTC TCAGAAGACC AAAGGGCAAT TGAGACTTTT CAACAAAGGG TAATATCCGG AAACCTCCTC GGATTCCATT GCCCAGCTAT CTGTCACTTT ATTGTGAAGA TAGTGGAAAA GGAAGGTGGC TCCTACAAAT GCCATCATTG CGATAAAGGA AAGGCCATCG TTGAAGATGC CTCTGCCGAC AGTGGTCCCA AAGATGGACC CCCACCCACG AGGAGCATCG TGGAAAAAGA AGACGTTCCA ACCACGTCTT CAAAGCAAGT GGATTGATGT GATAACATGG TGGAGCACGA CACACTTGTC TACTCCAAAA ATATCAAAGA TACAGTCTCA GAAGACCAAA GGGCAATTGA GACTTTTCAA CAAAGGGTAA TATCCGGAAA CCTCCTCGGA TTCCATTGCC CAGCTATCTG TCACTTTATT GTGAAGATAG TGGAAAAGGA AGGTGGCTCC TACAAATGCC ATCATTGCGA TAAAGGAAAG GCCATCGTTG AAGATGCCTC TGCCGACAGT GGTCCCAAAG ATGGACCCCC ACCCACGAGG AGCATCGTGG AAAAAGAAGA CGTTCCAACC ACGTCTTCAA AGCAAGTGGA TTGATGTGAT ATCTCCACTG ACGTAAGGGA TGACGCACAA TCCCACTATC CTTCGCAAGA CCCTTCCTCT ATATAAGGAA GTTCATTTCA TTTGGAGAGG ACCTCGACCT CAACACAACA TATACAAAAC AAACGAATCT CAAGCAATCA AGCATTCTAC TTCTATTGCA GCAATTTAAA TCATTTCTTT TAAAGCAAAA GCAATTTTCT GAAAATTTTC ACCATTTACG AACGATACTC GAGTAATCTA GATGGATTAC AAGGACCACG ACGGGGATTA CAAGGACCAC GACATTGATT ACAAGGATGA TGATGACAAG ATGGCTCCGA AGAAGAAGAG GAAGGTTGGC ATCCACGGGG TGCCAGCTGC TGACAAGAAG TACTCGATCG GCCTCGATAT TGGGACTAAC TCTGTTGGCT GGGCCGTGAT CACCGACGAG TACAAGGTGC CCTCAAAGAA GTTCAAGGTC CTGGGCAACA CCGATCGGCA TTCCATCAAG AAGAATCTCA TTGGCGCTCT CCTGTTCGAC AGCGGCGAGA CGGCTGAGGC TACGCGGCTC AAGCGCACCG CCCGCAGGCG GTACACGCGC AGGAAGAATC GCATCTGCTA CCTGCAGGAG ATTTTCTCCA ACGAGATGGC GAAGGTTGAC GATTCTTTCT TCCACAGGCT GGAGGAGTCA TTCCTCGTGG AGGAGGATAA GAAGCACGAG CGGCATCCAA TCTTCGGCAA CATTGTCGAC GAGGTTGCCT ACCACGAGAA GTACCCTACG ATCTACCATC TGCGGAAGAA GCTCGTGGAC TCCACAGATA AGGCGGACCT CCGCCTGATC TACCTCGCTC TGGCCCACAT GATTAAGTTC AGGGGCCATT TCCTGATCGA GGGGGATCTC AACCCGGACA ATAGCGATGT TGACAAGCTG TTCATCCAGC TCGTGCAGAC GTACAACCAG CTCTTCGAGG AGAACCCCAT TAATGCGTCA GGCGTCGACG CGAAGGCTAT CCTGTCCGCT AGGCTCTCGA AGTCTCGGCG CCTCGAGAAC CTGATCGCCC AGCTGCCGGG CGAGAAGAAG AACGGCCTGT TCGGGAATCT CATTGCGCTC AGCCTGGGGC TCACGCCCAA CTTCAAGTCG AATTTCGATC TCGCTGAGGA CGCCAAGCTG CAGCTCTCCA AGGACACATA CGACGATGAC CTGGATAACC TCCTGGCCCA GATCGGCGAT CAGTACGCGG ACCTGTTCCT CGCTGCCAAG AATCTGTCGG ACGCCATCCT CCTGTCTGAT ATTCTCAGGG TGAACACCGA GATTACGAAG GCTCCGCTCT CAGCCTCCAT GATCAAGCGC TACGACGAGC ACCATCAGGA TCTGACCCTC CTGAAGGCGC TGGTCAGGCA GCAGCTCCCC GAGAAGTACA AGGAGATCTT CTTCGATCAG TCGAAGAACG GCTACGCTGG GTACATTGAC GGCGGGGCCT CTCAGGAGGA GTTCTACAAG TTCATCAAGC CGATTCTGGA GAAGATGGAC GGCACGGAGG AGCTGCTGGT GAAGCTCAAT CGCGAGGACC TCCTGAGGAA GCAGCGGACA TTCGATAACG GCAGCATCCC ACACCAGATT CATCTCGGGG AGCTGCACGC TATCCTGAGG AGGCAGGAGG ACTTCTACCC TTTCCTCAAG GATAACCGCG AGAAGATCGA GAAGATTCTG ACTTTCAGGA TCCCGTACTA CGTCGGCCCA CTCGCTAGGG GCAACTCCCG CTTCGCTTGG ATGACCCGCA AGTCAGAGGA GACGATCACG CCGTGGAACT TCGAGGAGGT GGTCGACAAG GGCGCTAGCG CTCAGTCGTT CATCGAGAGG ATGACGAATT TCGACAAGAA CCTGCCAAAT GAGAAGGTGC TCCCTAAGCA CTCGCTCCTG TACGAGTACT TCACAGTCTA CAACGAGCTG ACTAAGGTGA AGTATGTGAC CGAGGGCATG AGGAAGCCGG CTTTCCTGTC TGGGGAGCAG AAGAAGGCCA TCGTGGACCT CCTGTTCAAG ACCAACCGGA AGGTCACGGT TAAGCAGCTC AAGGAGGACT ACTTCAAGAA GATTGAGTGC TTCGATTCGG TCGAGATCTC TGGCGTTGAG GACCGCTTCA ACGCCTCCCT GGGGACCTAC CACGATCTCC TGAAGATCAT TAAGGATAAG GACTTCCTGG ACAACGAGGA GAATGAGGAT ATCCTCGAGG ACATTGTGCT GACACTCACT CTGTTCGAGG ACCGGGAGAT GATCGAGGAG CGCCTGAAGA CTTACGCCCA TCTCTTCGAT GACAAGGTCA TGAAGCAGCT CAAGAGGAGG AGGTACACCG GCTGGGGGAG GCTGAGCAGG AAGCTCATCA ACGGCATTCG GGACAAGCAG TCCGGGAAGA CGATCCTCGA CTTCCTGAAG AGCGATGGCT TCGCGAACCG CAATTTCATG CAGCTGATTC ACGATGACAG CCTCACATTC AAGGAGGATA TCCAGAAGGC TCAGGTGAGC GGCCAGGGGG ACTCGCTGCA CGAGCATATC GCGAACCTCG CTGGCTCGCC AGCTATCAAG AAGGGGATTC TGCAGACCGT GAAGGTTGTG GACGAGCTGG TGAAGGTCAT GGGCAGGCAC AAGCCTGAGA ACATCGTCAT TGAGATGGCC CGGGAGAATC AGACCACGCA GAAGGGCCAG AAGAACTCAC GCGAGAGGAT GAAGAGGATC GAGGAGGGCA TTAAGGAGCT GGGGTCCCAG ATCCTCAAGG AGCACCCGGT GGAGAACACG CAGCTGCAGA ATGAGAAGCT CTACCTGTAC TACCTCCAGA ATGGCCGCGA TATGTATGTG GACCAGGAGC TGGATATTAA CAGGCTCAGC GATTACGACG TCGATCATAT CGTTCCACAG TCATTCCTGA AGGATGACTC CATTGACAAC AAGGTCCTCA CCAGGTCGGA CAAGAACCGG GGCAAGTCTG ATAATGTTCC TTCAGAGGAG GTCGTTAAGA AGATGAAGAA CTACTGGCGC CAGCTCCTGA ATGCCAAGCT GATCACGCAG CGGAAGTTCG ATAACCTCAC AAAGGCTGAG AGGGGCGGGC TCTCTGAGCT GGACAAGGCG GGCTTCATCA AGAGGCAGCT GGTCGAGACA CGGCAGATCA CTAAGCACGT TGCGCAGATT CTCGACTCAC GGATGAACAC TAAGTACGAT GAGAATGACA AGCTGATCCG CGAGGTGAAG GTCATCACCC TGAAGTCAAA GCTCGTCTCC GACTTCAGGA AGGATTTCCA GTTCTACAAG GTTCGGGAGA TCAACAATTA CCACCATGCC CATGACGCGT ACCTGAACGC GGTGGTCGGC ACAGCTCTGA TCAAGAAGTA CCCAAAGCTC GAGAGCGAGT TCGTGTACGG GGACTACAAG GTTTACGATG TGAGGAAGAT GATCGCCAAG TCGGAGCAGG AGATTGGCAA GGCTACCGCC AAGTACTTCT TCTACTCTAA CATTATGAAT TTCTTCAAGA CAGAGATCAC TCTGGCCAAT GGCGAGATCC GGAAGCGCCC CCTCATCGAG ACGAACGGCG AGACGGGGGA GATCGTGTGG GACAAGGGCA GGGATTTCGC GACCGTCAGG AAGGTTCTCT CCATGCCACA AGTGAATATC GTCAAGAAGA CAGAGGTCCA GACTGGCGGG TTCTCTAAGG AGTCAATTCT GCCTAAGCGG AACAGCGACA AGCTCATCGC CCGCAAGAAG GACTGGGATC CGAAGAAGTA CGGCGGGTTC GACAGCCCCA CTGTGGCCTA CTCGGTCCTG GTTGTGGCGA AGGTTGAGAA GGGCAAGTCC AAGAAGCTCA AGAGCGTGAA GGAGCTGCTG GGGATCACGA TTATGGAGCG CTCCAGCTTC GAGAAGAACC CGATCGATTT CCTGGAGGCG AAGGGCTACA AGGAGGTGAA GAAGGACCTG ATCATTAAGC TCCCCAAGTA CTCACTCTTC GAGCTGGAGA ACGGCAGGAA GCGGATGCTG GCTTCCGCTG GCGAGCTGCA GAAGGGGAAC GAGCTGGCTC TGCCGTCCAA GTATGTGAAC TTCCTCTACC TGGCCTCCCA CTACGAGAAG CTCAAGGGCA GCCCCGAGGA CAACGAGCAG AAGCAGCTGT TCGTCGAGCA GCACAAGCAT TACCTCGACG AGATCATTGA GCAGATTTCC GAGTTCTCCA AGCGCGTGAT CCTGGCCGAC GCGAATCTGG ATAAGGTCCT CTCCGCGTAC AACAAGCACC GCGACAAGCC AATCAGGGAG CAGGCTGAGA ATATCATTCA TCTCTTCACC CTGACGAACC TCGGCGCCCC TGCTGCTTTC AAGTACTTCG ACACAACTAT CGATCGCAAG AGGTACACAA GCACTAAGGA GGTCCTGGAC GCGACCCTCA TCCACCAGTC GATTACCGGC CTCTACGAGA CGCGCATCGA CCTGTCTCAG CTCGGGGGCG ACAAGCGGCC AGCGGCGACG AAGAAGGCGG GGCAGGCGAA GAAGAAGAAG TGACTCCAAA AATATCAAAG ATACAGTCTC AGAAGACCAA AGGGCAATTG AGACTTTTCA ACAAAGGGTA ATATCCGGAA ACCTCCTCGG ATTCCATTGC CCAGCTATCT GTCACTTTAT TGTGAAGATA GTGGAAAAGG AAGGTGGCTC CTACAAATGC CATCATTGCG ATAAAGGAAA GGCCATCGTT GAAGATGCCT CTGCCGACAG TGGTCCCAAA GATGGACCCC CACCCACGAG GAGCATCGTG GAAAAAGAAG ACGTTCCAAC CACGTCTTCA AAGCAAGTGG ATTGATGTGA TATCTCCACT GACGTAAGGG ATGACGCACA ATCCCACTAT CCTTCGCAAG ACCCTTCCTC TATATAAGGA AGTTCATTTC ATTTGGAGAG AACACGGGGG ACTCTAGAGG ATCCCCGGGA TGGCCTCCTC CGAGAACGTC ATCACCGAGT TCATGCGCTT CAAGGTGCGC ATGGAGGGCA CCGTGAACGG CCACGAGTTC GAGATCGAGG GCGAGGGCGA GGGCCGCCCC TACGAGGGCC ACAACACCGT GAAGCTGAAG GTGACCAAGG GCGGCCCCCT GCCCTTCGCC TGGGACATCC TGTCCCCCCA GTTCCAGTAC GGCTCCAAGG TGTACGTGAA GCACCCCGCC GACATCCCCG ACTACAAGAA GCTGTCCTTC CCCGAGGGCT TCAAGTGGGA GCGCGTGATG AACTTCGAGG ACGGCGGCGT GGCGACCGTG ACCCAGGACT CCTCCCTGCA GGACGGCTGC TTCATCTACA AGGTGAAGTT CATCGGCGTG AACTTCCCCT CCGACGGCCC CGTGATGCAG AAGAAGACCA TGGGCTGGGA GGCCTCCACC GAGCGCCTGT ACCCCCGCGA CGGCGTGCTG AAGGGCGAGA CCCACAAGGC CCTGAAGCTG AAGGACGGCG GCCACTACCT GGTGGAGTTC AAGTCCATCT ACATGGCCAA GAAGCCCGTG CAGCTGCCCG GCTACTACTA CGTGGACGCC AAGCTGGACA TCACCTCCCA CAACGAGGAC TACACCATCG TGGAGCAGTA CGAGCGCACC GAGGGCCGCC ACCACCTGTT CCTGTAGGCT CAGAGCTTTC GTTCGTATCA TCGGTTTCGA CAACGTTCGT CAAGTTCAAT GCATCAGTTT CATTGCGCAC ACACCAGAAT CCTACTGAGT TTGAGTATTA TGGCATTGGG AAAACTGTTT TTCTTGTACC ATTTGTTGTG CTTGTAATTT ACTGTGTTTT TTATTCGGTT TTCGCTATCG AACTGTGAAA TGGAAATGGA TGGAGAAGAG TTAATGAATG ATATGGTCCT TTTGTTCATT CTCAAATTAA TATTATTTGT TTTTTCTCTT ATTTGTTGTG TGTTGAATTT GAAATTATAA GAGATATGCA AACATTTTGT TTTGAGTAAA AATGTGTCAA ATCGTGGCCT CTAATGACCG AAGTTAATAT GAGGAGTAAA ACACTTGTAG TTGTACCATT ATGCTTATTC ACTAGGCAAC AAATATATTT TCAGACCTAG AAAAGCTGCA AATGTTACTG AATACAAGTA TGTCCTCTTG TGTTTTAGAC ATTTATGAAC TTTCCTTTAT GTAATTTTCC AGAATCCTTG TCAGATTCTA ATCATTGCTT TATAATTATA GTTATACTCA TGGATTTGTA GTTGAGTATG AAAATATTTT TTAATGCATT TTATGACTTG CCAATTGATT GACAACGAAT TCGTAATCAT GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGCTAG AGCAGCTTGC CAACATGGTG GAGCACGACA CTCTCGTCTA CTCCAAGAAT ATCAAAGATA CAGTCTCAGA AGACCAAAGG GCTATGAACC GCAACGTTGA AGGAGCCACT GAGCCGCGGG TTTCTGGAGT TTAATGAGCT AAGCACATAC GTCAGAAACC ATTATTGCGC GTTCAAAAGT CGCCTAAGGT CACTATCAGC TAGCAAATAT TTCTTGTCAA AAATGCTCCA CTGACGTTCC ATAAATTCCC CTCGGTATCC AATTAGAGTC TCATATTCAC TCTCAACTCG ATCGAGGGGA TCTACCATGA GCCCAGAACG ACGCCCGGCC GACATCCGCC GTGCCACCGA GGCGGACATG CCGGCGGTCT GCACCATCGT CAACCACTAC ATCGAGACAA GCACGGTCAA CTTCCGTACC GAGCCGCAGG AACCGCAGGA GTGGACGGAC GACCTCGTCC GTCTGCGGGA GCGCTATCCC TGGCTCGTCG CCGAGGTGGA CGGCGAGGTC GCCGGCATCG CCTACGCGGG TCCCTGGAAG GCACGCAACG CCTACGACTG GACGGCCGAG TCGACCGTGT ACGTCTCCCC CCGCCACCAG CGGACGGGAC TGGGCTCCAC GCTCTACACC CACCTGCTGA AGTCCCTGGA GGCACAGGGC TTCAAGAGCG TGGTCGCTGT CATCGGGCTG CCCAACGACC CGAGCGTGCG CATGCACGAG GCGCTCGGAT ATGCCCCCCG CGGCATGCTG CGGGCGGCCG GCTTCAAGCA CGGGAACTGG CATGACGTGG GTTTCTGGCA GCTGGACTTC AGCCTGCCGG TGCCGCCCCG TCCGGTCCTG CCCGTCACCG AAATCTGATG ACCCCTAGAG TCAAGCAGAT CGTTAAAACA TTTGGCAATA AAGTTTCTTA AGATTGAATC CTGTTGCCGG TCTTGCGATG ATTATCATAT AATTTCTGTT GAATTACGTT AAGCATGTAA TAATTAACAT GTAATGCATG ACGTTATTTA TGAGATGGGT TTTTATGATT AGAGTCCCGC AATTATACAT TTAATACGCG ATAGAAAACA AAATATAGCG CGCAAACTAG GATAAATTAT CGCGCGCGGT GTCATCTATG TTACTAGATC CCGCGGTTTC AAAATCGGCT CCGTCGATAC TATGTTATAC GCCAACTTTG AAAACAACTT TGAAAAAGCT GTTTTCTGGT ATTTAAGGTT TTAGAATGCA AGGAACAGTG AATTGGAGTT CGTCTTGTTA TAATTAGCTT CTTGGGGTAT CTTTAAATAC TGTAGAAAAG AGGAAGGAAA TAATAAATGG CTAAAATGAG AATATCACCG GAATTGAAAA AACTGATCGA AAAATACCGC TGCGTAAAAG ATACGGAAGG AATGTCTCCT GCTAAGGTAT ATAAGCTGGT GGGAGAAAAT GAAAACCTAT ATTTAAAAAT GACGGACAGC CGGTATAAAG GGACCACCTA TGATGTGGAA CGGGAAAAGG ACATGATGCT ATGGCTGGAA GGAAAGCTGC CTGTTCCAAA GGTCCTGCAC TTTGAACGGC ATGATGGCTG GAGCAATCTG CTCATGAGTG AGGCCGATGG CGTCCTTTGC TCGGAAGAGT ATGAAGATGA ACAAAGCCCT GAAAAGATTA TCGAGCTGTA TGCGGAGTGC ATCAGGCTCT TTCACTCCAT CGACATATCG GATTGTCCCT ATACGAATAG CTTAGACAGC CGCTTAGCCG AATTGGATTA CTTACTGAAT AACGATCTGG CCGATGTGGA TTGCGAAAAC TGGGAAGAAG ACACTCCATT TAAAGATCCG CGCGAGCTGT ATGATTTTTT AAAGACGGAA AAGCCCGAAG AGGAACTTGT CTTTTCCCAC GGCGACCTGG GAGACAGCAA CATCTTTGTG AAAGATGGCA AAGTAAGTGG CTTTATTGAT CTTGGGAGAA GCGGCAGGGC GGACAAGTGG TATGACATTG CCTTCTGCGT CCGGTCGATC AGGGAGGATA TCGGGGAAGA ACAGTATGTC GAGCTATTTT TTGACTTACT GGGGATCAAG CCTGATTGGG AGAAAATAAA ATATTATATT TTACTGGATG AATTGTTTTA GTACCTAGAA TGCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGGGTGCCT TGATGTGGGC GCCGGCGGTC GAGTGGCGAC GGCGCGGCTT GTCCGCGCCC TGGTAGATTG CCTGGCCGTA GGCCAGCCAT TTTTGAGCGG CCAGCGGCCG CGATAGGCCG ACGCGAAGCG GCGGGGCGTA GGGAGCGCAG CGACCGAAGG GTAGGCGCTT TTTGCAGCTC TTCGGCTGTG CGCTGGCCAG ACAGTTATGC ACAGGCCAGG CGGGTTTTAA GAGTTTTAAT AAGTTTTAAA GAGTTTTAGG CGGAAAAATC GCCTTTTTTC TCTTTTATAT CAGTCACTTA CATGTGTGAC CGGTTCCCAA TGTACGGCTT TGGGTTCCCA ATGTACGGGT TCCGGTTCCC AATGTACGGC TTTGGGTTCC CAATGTACGT GCTATCCACA GGAAACAGAC CTTTTCGACC TTTTTCCCCT GCTAGGGCAA TTTGCCCTAG CATCTGCTCC GTACATTAGG AACCGGCGGA TGCTTCGCCC TCGATCAGGT TGCGGTAGCG CATGACTAGG ATCGGGCCAG CCTGCCCCGC CTCCTCCTTC AAATCGTACT CCGGCAGGTC ATTTGACCCG ATCAGCTTGC GCACGGTGAA ACAGAACTTC TTGAACTCTC CGGCGCTGCC ACTGCGTTCG TAGATCGTCT TGAACAACCA TCTGGCTTCT GCCTTGCCTG CGGCGCGGCG TGCCAGGCGG TAGAGAAAAC GGCCGATGCC GGGATCGATC AAAAAGTAAT CGGGGTGAAC CGTCAGCACG TCCGGGTTCT TGCCTTCTGT GATCTCGCGG TACATCCAAT CAGCTAGCTC GATCTCGATG TACTCCGGCC GCCCGGTTTC GCTCTTTACG ATCTTGTAGC GGCTAATCAA GGCTTCACCC TCGGATACCG TCACCAGGCG GCCGTTCTTG GCCTTCTTCG TACGCTGCAT GGCAACGTGC GTGGTGTTTA ACCGAATGCA GGTTTCTACC AGGTCGTCTT TCTGCTTTCC GCCATCGGCT CGCCGGCAGA ACTTGAGTAC GTCCGCAACG TGTGGACGGA ACACGCGGCC GGGCTTGTCT CCCTTCCCTT CCCGGTATCG GTTCATGGAT TCGGTTAGAT GGGAAACCGC CATCAGTACC AGGTCGTAAT CCCACACACT GGCCATGCCG GCCGGCCCTG CGGAAACCTC TACGTGCCCG TCTGGAAGCT CGTAGCGGAT CACCTCGCCA GCTCGTCGGT CACGCTTCGA CAGACGGAAA ACGGCCACGT CCATGATGCT GCGACTATCG CGGGTGCCCA CGTCATAGAG CATCGGAACG AAAAAATCTG GTTGCTCGTC GCCCTTGGGC GGCTTCCTAA TCGACGGCGC ACCGGCTGCC GGCGGTTGCC GGGATTCTTT GCGGATTCGA TCAGCGGCCG CTTGCCACGA TTCACCGGGG CGTGCTTCTG CCTCGATGCG TTGCCGCTGG GCGGCCTGCG CGGCCTTCAA CTTCTCCACC AGGTCATCAC CCAGCGCCGC GCCGATTTGT ACCGGGCCGG ATGGTTTGCG ACCGCTCACG CCGATTCCTC GGGCTTGGGG GTTCCAGTGC CATTGCAGGG CCGGCAGGCA ACCCAGCCGC TTACGCCTGG CCAACCGCCC GTTCCTCCAC ACATGGGGCA TTCCACGGCG TCGGTGCCTG GTTGTTCTTG ATTTTCCATG CCGCCTCCTT TAGCCGCTAA AATTCATCTA CTCATTTATT CATTTGCTCA TTTACTCTGG TAGCTGCGCG ATGTATTCAG ATAGCAGCTC GGTAATGGTC TTGCCTTGGC GTACCGCGTA CATCTTCAGC TTGGTGTGAT CCTCCGCCGG CAACTGAAAG TTGACCCGCT TCATGGCTGG CGTGTCTGCC AGGCTGGCCA ACGTTGCAGC CTTGCTGCTG CGTGCGCTCG GACGGCCGGC ACTTAGCGTG TTTGTGCTTT TGCTCATTTT CTCTTTACCT CATTAACTCA AATGAGTTTT GATTTAATTT CAGCGGCCAG CGCCTGGACC TCGCGGGCAG CGTCGCCCTC GGGTTCTGAT TCAAGAACGG TTGTGCCGGC GGCGGCAGTG CCTGGGTAGC TCACGCGCTG CGTGATACGG GACTCAAGAA TGGGCAGCTC GTACCCGGCC AGCGCCTCGG CAACCTCACC GCCGATGCGC GTGCCTTTGA TCGCCCGCGA CACGACAAAG GCCGCTTGTA GCCTTCCATC CGTGACCTCA ATGCGCTGCT TAACCAGCTC CACCAGGTCG GCGGTGGCCC ATATGTCGTA AGGGCTTGGC TGCACCGGAA TCAGCACGAA GTCGGCTGCC TTGATCGCGG ACACAGCCAA GTCCGCCGCC TGGGGCGCTC CGTCGATCAC TACGAAGTCG CGCCGGCCGA TGGCCTTCAC GTCGCGGTCA ATCGTCGGGC GGTCGATGCC GACAACGGTT AGCGGTTGAT CTTCCCGCAC GGCCGCCCAA TCGCGGGCAC TGCCCTGGGG ATCGGAATCG ACTAACAGAA CATCGGCCCC GGCGAGTTGC AGGGCGCGGG CTAGATGGGT TGCGATGGTC GTCTTGCCTG ACCCGCCTTT CTGGTTAAGT ACAGCGATAA CCTTCATGCG TTCCCCTTGC GTATTTGTTT ATTTACTCAT CGCATCATAT ACGCAGCGAC CGCATGACGC AAGCTGTTTT ACTCAAATAC ACATCACCTT TTTAGACGGC GGCGCTCGGT TTCTTCAGCG GCCAAGCTGG CCGGCCAGGC CGCCAGCTTG GCATCAGACA AACCGGCCAG GATTTCATGC AGCCGCACGG TTGAGACGTG CGCGGGCGGC TCGAACACGT ACCCGGCCGC GATCATCTCC GCCTCGATCT CTTCGGTAAT GAAAAACGGT TCGTCCTGGC CGTCCTGGTG CGGTTTCATG CTTGTTCCTC TTGGCGTTCA TTCTCGGCGG CCGCCAGGGC GTCGGCCTCG GTCAATGCGT CCTCACGGAA GGCACCGCGC CGCCTGGCCT CGGTGGGCGT CACTTCCTCG CTGCGCTCAA GTGCGCGGTA CAGGGTCGAG CGATGCACGC CAAGCAGTGC AGCCGCCTCT TTCACGGTGC GGCCTTCCTG GTCGATCAGC TCGCGGGCGT GCGCGATCTG TGCCGGGGTG AGGGTAGGGC GGGGGCCAAA CTTCACGCCT CGGGCCTTGG CGGCCTCGCG CCCGCTCCGG GTGCGGTCGA TGATTAGGGA ACGCTCGAAC TCGGCAATGC CGGCGAACAC GGTCAACACC ATGCGGCCGG CCGGCGTGGT GGTGTCGGCC CACGGCTCTG CCAGGCTACG CAGGCCCGCG CCGGCCTCCT GGATGCGCTC GGCAATGTCC AGTAGGTCGC GGGTGCTGCG GGCCAGGCGG TCTAGCCTGG TCACTGTCAC AACGTCGCCA GGGCGTAGGT GGTCAAGCAT CCTGGCCAGC TCCGGGCGGT CGCGCCTGGT GCCGGTGATC TTCTCGGAAA ACAGCTTGGT GCAGCCGGCC GCGTGCAGTT CGGCCCGTTG GTTGGTCAAG TCCTGGTCGT CGGTGCTGAC GCGGGCATAG CCCAGCAGGC CAGCGGCGGC GCTCTTGTTC ATGGCGTAAT GTCTCCGGTT CTAGTCGCAA GTATTCTACT TTATGCGACT AAAACACGCG ACAAGAAAAC GCCAGGAAAA GGGCAGGGCG GCAGCCTGTC GCGTAACTTA GGACTTGTGC GACATGTCGT TTTCAGAAGA CGGCTGCACT GAACGTCAGA AGCCGACTGC ACTATAGCAG CGGAGGGGTT GGATCAAAGT ACTTTGATCC CGAGGGGAAC CCTGTGGTTG GCATGCACAT ACAAATGGAC GAACGGATAA ACCTTTTCAC GCCCTTTTAA ATATCCGATT ATTCTAA
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