NM_000069.3;CACNL1A3; Cav1.1; CCHL1A3; CMYO18; CMYP18;DHPRM; HOKPP; HOKPP1; hypoPP; MHS5; TTPP1;
100μg liquid plasmid(Instantly):$149
| name | pLV3-CMV-CACNA1S(human)-3×FLAG-Puro | another name | apLV3-CMV-CACNA1S(human)-3×FLAG-Puro |
| Insert Size (bp) | 5619bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | pLV3-CMV-MCS-3×FLAG-Puro | 5′ sequencing primer | CMV-F |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT AGAGCTAGCG AATTCGCCAC CATGGAGCCA TCCTCACCCC AGGATGAAGG CCTGAGGAAG AAACAGCCCA AGAAGCCAGT TCCTGAGATT CTGCCAAGGC CACCCCGGGC CTTGTTCTGC CTGACCCTGG AGAACCCCCT GAGGAAGGCC TGCATCAGCA TTGTAGAATG GAAGCCCTTC GAGACGATCA TCTTGCTCAC CATCTTTGCC AATTGTGTGG CCCTGGCCGT GTACCTGCCC ATGCCGGAAG ATGACAACAA CTCTCTGAAC CTCGGCCTGG AGAAGCTGGA GTATTTCTTC CTCATTGTCT TCTCGATTGA AGCCGCCATG AAGATCATTG CCTACGGCTT CTTATTCCAC CAGGACGCTT ACCTGCGCAG TGGCTGGAAT GTGCTGGACT TCACCATTGT CTTCCTGGGG GTCTTCACCG TGATTCTGGA ACAGGTTAAC GTCATCCAAA GCCACACAGC CCCAATGAGC AGCAAAGGAG CCGGCTTGGA TGTCAAGGCC CTCAGAGCCT TCCGAGTGCT CAGACCCCTC CGGCTGGTGT CGGGGGTGCC TAGCCTGCAG GTGGTCCTGA ACTCCATCTT CAAGGCCATG CTCCCCCTCT TTCACATCGC CCTGCTGGTC CTCTTTATGG TCATCATCTA TGCCATCATC GGGCTGGAGC TCTTCAAGGG CAAGATGCAC AAGACCTGCT ACTTCATTGG TACAGATATC GTGGCCACGG TGGAGAATGA AGAGCCATCG CCCTGCGCCA GGACGGGCTC AGGGCGCCGG TGCACCATCA ATGGCAGTGA GTGCCGGGGC GGCTGGCCAG GGCCCAACCA TGGCATCACC CACTTCGACA ACTTCGGCTT CTCCATGCTC ACCGTGTACC AGTGCATTAC CATGGAGGGA TGGACTGACG TCCTTTACTG GGTCAATGAT GCCATCGGGA ATGAGTGGCC CTGGATCTAT TTTGTCACCC TCATTTTGCT GGGATCCTTC TTCATCCTCA ACCTGGTGCT GGGTGTCCTG AGTGGGGAAT TCACCAAGGA GCGGGAGAAG GCCAAGTCCA GGGGAACCTT CCAGAAGCTC CGGGAGAAGC AGCAACTAGA TGAGGACCTT CGGGGCTACA TGAGCTGGAT CACGCAGGGC GAGGTCATGG ATGTTGAGGA CTTCAGAGAA GGAAAACTGT CTTTGGATGA AGGTGGCTCT GACACAGAGA GCCTGTATGA AATTGCAGGC TTGAACAAAA TCATCCAGTT CATCCGACAT TGGAGGCAGT GGAACCGCAT CTTTCGCTGG AAGTGCCATG ACATCGTGAA GTCCAAGGTC TTCTATTGGC TGGTGATTCT CATCGTTGCC CTCAACACCC TGTCTATCGC CTCAGAGCAC CACAACCAGC CTCTCTGGCT GACCCGTTTG CAAGACATTG CCAACCGGGT GCTGCTGTCC CTCTTCACCA CTGAGATGCT GATGAAGATG TACGGGCTGG GCCTGCGCCA GTACTTCATG TCTATCTTCA ACCGCTTCGA CTGCTTCGTG GTGTGTAGCG GTATCCTGGA GATCCTGCTG GTGGAGTCGG GTGCCATGAC ACCCCTGGGC ATCTCCGTGC TCCGCTGCAT CCGCCTCCTG AGGATCTTCA AGATCACCAA ATATTGGACG TCGCTGAGCA ACCTGGTGGC ATCCCTGCTC AACTCCATCC GCTCCATCGC CTCCCTGCTG CTGCTGCTCT TCCTCTTCAT CGTCATCTTC GCCCTCCTGG GCATGCAGCT CTTTGGGGGG AGGTATGACT TTGAAGACAC AGAAGTACGG CGCAGCAACT TTGACAACTT TCCCCAAGCC CTCATCAGCG TCTTCCAGGT ACTGACAGGG GAAGACTGGA CCTCAATGAT GTACAATGGG ATCATGGCCT ACGGCGGGCC GTCCTACCCT GGCATGCTTG TGTGCATTTA CTTCATCATC CTTTTCGTCT GTGGCAACTA CATCCTGCTC AATGTCTTCC TGGCCATTGC CGTGGACAAC CTGGCCGAGG CGGAGAGCCT GACTTCTGCC CAGAAGGCCA AGGCTGAGGA GAAAAAACGC AGGAAGATGT CCAAGGGTCT CCCAGACAAG TCAGAAGAGG AGAAGTCAAC GATGGCCAAG AAGCTGGAGC AGAAACCCAA GGGTGAGGGC ATCCCCACCA CTGCCAAGCT GAAAATCGAT GAGTTTGAAT CTAATGTCAA TGAGGTGAAG GATCCCTACC CCTCAGCCGA CTTCCCAGGG GATGACGAGG AAGATGAGCC TGAGATCCCG CTGAGCCCCC GACCACGTCC CCTGGCTGAG CTGCAGCTGA AAGAGAAGGC CGTGCCCATT CCAGAAGCCA GCTCCTTCTT CATCTTCAGC CCCACCAATA AGATCCGTGT CCTGTGTCAC CGCATCGTCA ATGCCACCTG GTTTACCAAC TTCATCCTGC TCTTCATCCT GCTCAGCAGC GCTGCACTGG CTGCGGAAGA CCCCATCCGG GCTGATTCCA TGAGAAATCA GATCCTTAAA CACTTTGACA TCGGGTTCAC CTCTGTCTTC ACTGTGGAGA TTGTCCTCAA GATGACGACC TACGGAGCCT TCCTGCACAA GGGTTCCTTC TGCCGCAATT ACTTCAACAT GCTGGACCTG CTGGTGGTGG CCGTGTCCCT CATCTCCATG GGACTTGAGT CCAGTGCCAT CTCCGTGGTG AAGATCCTGA GGGTGCTGAG GGTGCTCCGA CCACTCAGAG CCATCAACAG AGCCAAGGGG TTGAAGCACG TGGTGCAGTG CATGTTCGTG GCCATCAGCA CCATCGGGAA CATCGTGCTG GTCACTACCC TCCTACAGTT CATGTTTGCC TGCATCGGCG TCCAGCTCTT CAAGGGGAAG TTCTTCAGGT GCACCGACTT GTCCAAGATG ACAGAGGAGG AGTGCAGGGG CTACTACTAC GTGTACAAGG ACGGGGACCC CATGCAGATA GAGCTGCGTC ACCGCGAGTG GGTACACAGC GACTTCCACT TCGACAATGT GCTCTCAGCC ATGATGTCCC TCTTCACGGT CTCCACCTTC GAGGGATGGC CTCAGCTGCT GTACAAGGCC ATAGACTCCA ATGCGGAGGA CGTGGGTCCC ATCTACAACA ACCGTGTGGA GATGGCCATC TTCTTCATCA TCTACATCAT CCTCATTGCC TTCTTCATGA TGAACATCTT TGTGGGCTTC GTCATTGTCA CCTTCCAGGA GCAGGGAGAG ACTGAGTACA AGAACTGTGA GCTGGACAAG AACCAGCGCC AATGTGTACA GTATGCCCTG AAGGCCCGCC CACTGAGGTG CTACATTCCC AAAAACCCAT ACCAGTACCA GGTGTGGTAC ATTGTCACCT CCTCCTACTT TGAATACCTG ATGTTTGCCC TCATCATGCT CAACACCATC TGCCTCGGCA TGCAGCACTA CAACCAGTCG GAGCAGATGA ACCACATCTC AGACATCCTC AATGTGGCCT TCACTATCAT CTTCACCCTG GAGATGATCC TCAAGCTCAT GGCCTTCAAG GCCAGGGGCT ACTTTGGAGA CCCCTGGAAT GTGTTTGACT TCCTGATTGT CATTGGCAGC ATCATTGATG TCATCCTCAG TGAGATCGAC ACTTTCCTGG CCTCCAGCGG GGGACTGTAT TGCCTGGGTG GAGGCTGCGG GAACGTTGAC CCAGATGAGA GTGCCCGCAT CTCCAGCGCC TTCTTCCGCC TGTTCCGTGT CATGAGGCTG ATCAAGCTGC TGAGCCGGGC AGAAGGAGTG CGAACCCTCC TGTGGACGTT CATCAAGTCC TTCCAGGCCC TACCCTACGT GGCTCTGCTC ATCGTCATGC TCTTCTTCAT CTACGCTGTC ATCGGCATGC AGATGTTTGG GAAGATCGCC TTGGTGGATG GGACCCAAAT AAACCGGAAC AACAACTTCC AGACCTTCCC ACAAGCTGTG CTACTGCTCT TCAGGTGTGC AACAGGTGAG GCCTGGCAGG AGATCCTACT GGCCTGCAGC TATGGGAAGC TGTGTGACCC AGAGTCGGAC TATGCCCCAG GGGAGGAGTA CACATGTGGC ACCAACTTTG CATACTACTA CTTCATCAGC TTCTACATGC TCTGTGCCTT CCTGGTCATC AACCTCTTTG TGGCTGTCAT CATGGACAAT TTTGACTACC TCACCCGGGA CTGGTCCATC CTGGGCCCTC ATCACCTGGA TGAGTTCAAG GCCATCTGGG CAGAGTATGA CCCAGAGGCT AAGGGGAGAA TCAAACACCT GGACGTGGTG ACCCTGCTGA GAAGGATTCA GCCCCCTCTG GGCTTTGGGA AGTTCTGCCC ACATCGGGTA GCTTGTAAGC GGCTGGTGGG CATGAACATG CCCCTGAACA GCGACGGCAC AGTCACCTTC AATGCCACAC TCTTTGCCCT GGTCCGCACG GCACTCAAGA TCAAGACGGA AGGTAACTTT GAGCAGGCCA ACGAGGAGCT GAGGGCCATC ATCAAGAAGA TCTGGAAGAG AACCAGCATG AAGCTCTTGG ACCAGGTCAT CCCTCCAATA GGAGATGATG AGGTGACAGT GGGGAAGTTC TACGCCACAT TCCTCATCCA GGAGCACTTC CGGAAGTTCA TGAAACGCCA AGAGGAGTAT TATGGCTATC GGCCCAAGAA GGACATTGTA CAGATCCAGG CAGGGCTGCG GACCATTGAG GAAGAGGCAG CCCCCGAGAT CTGTCGCACG GTCTCAGGAG ACCTGGCTGC TGAGGAGGAG CTGGAGAGAG CCATGGTGGA GGCTGCGATG GAGGAGGGAA TATTCCGGAG GACTGGAGGC CTGTTTGGCC AGGTGGACAA CTTCCTGGAA AGGACCAACT CCCTGCCCCC CGTCATGGCC AATCAGAGAC CCCTCCAGTT TGCTGAGATA GAGATGGAAG AGATGGAGTC ACCTGTCTTC TTGGAGGACT TCCCACAAGA TCCACGCACC AACCCCCTGG CTCGTGCCAA TACCAACAAT GCCAACGCCA ATGTCGCCTA TGGCAACAGC AACCATAGCA ACAGCCATGT GTTTTCCAGT GTCCACTATG AAAGGGAGTT CCCAGAAGAG ACAGAGACGC CTGCTACCAG AGGACGAGCC CTTGGCCAAC CCTGCAGGGT CCTGGGACCC CACAGCAAAC CCTGTGTGGA GATGCTGAAG GGACTGCTGA CCCAGAGGGC AATGCCCAGA GGCCAGGCAC CTCCTGCCCC CTGCCAGTGC CCCAGGGTGG AGTCCTCCAT GCCTGAGGAC AGAAAGAGCT CCACACCAGG GTCTCTTCAT GAGGAGACAC CCCACAGCAG GAGCACCAGG GAGAATACTT CCAGGTGCTC AGCACCAGCT ACAGCCCTGC TGATCCAAAA GGCTCTGGTT CGAGGGGGCC TGGGCACCTT GGCAGCTGAT GCAAACTTCA TCATGGCAAC AGGCCAGGCC CTGGCAGATG CCTGCCAAAT GGAACCAGAG GAAGTGGAGA TCATGGCAAC AGAGCTACTG AAAGGACGAG AGGCCCCAGA GGGCATGGCC AGCTCCCTGG GATGCCTGAA CCTCGGGTCC TCCCTGGGCA GCCTCGACCA ACACCAGGGC TCCCAGGAGA CCCTTATTCC TCCAAGGCTG GGATCCGACT ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA CTACAAGGAT GACGATGACA AGTAGCGGCC GCGAAGGATC TGCGATCGCT CCGGTGCCCG TCAGTGGGCA GAGCGCACAT CGCCCACAGT CCCCGAGAAG TTGGGGGGAG GGGTCGGCAA TTGAACGGGT GCCTAGAGAA GGTGGCGCGG GGTAAACTGG GAAAGTGATG TCGTGTACTG GCTCCGCCTT TTTCCCGAGG GTGGGGGAGA ACCGTATATA AGTGCAGTAG TCGCCGTGAA CGTTCTTTTT CGCAACGGGT TTGCCGCCAG AACACAGCTG AAGCTTCGAG GGGCTCGCAT CTCTCCTTCA CGCGCCCGCC GCCCTACCTG AGGCCGCCAT CCACGCCGGT TGAGTCGCGT TCTGCCGCCT CCCGCCTGTG GTGCCTCCTG AACTGCGTCC GCCGTCTAGG TAAGTTTAAA GCTCAGGTCG AGACCGGGCC TTTGTCCGGC GCTCCCTTGG AGCCTACCTA GACTCAGCCG GCTCTCCACG CTTTGCCTGA CCCTGCTTGC TCAACTCTAC GTCTTTGTTT CGTTTTCTGT TCTGCGCCGT TACAGATCCA AGCTGTGACC GGCGCCTACG CTAGATGACC GAGTACAAGC CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCCGGGC CGTACGCACC CTCGCCGCCG CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGACCC GGACCGCCAC ATCGAGCGGG TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG CCACCGTCGG CGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC CCGGAGTGGA GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC GAGGTGCCCG AAGGACCGCG CACCTGGTGC ATGACCCGCA AGCCCGGTGC CTGAGTCGAC AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAATCAT CGTCCTTTCC TTGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCC TGGTACCTTT AAGACCAATG ACTTACAAGG CAGCTGTAGA TCTTAGCCAC TTTTTAAAAG AAAAGGGGGG ACTGGAAGGG CTAATTCACT CCCAACGAAA ATAAGATCTG CTTTTTGCTT GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT TTAGTCAGTG TGGAAAATCT CTAGCAGTAG TAGTTCATGT CATCTTATTA TTCAGTATTT ATAACTTGCA AAGAAATGAA TATCAGAGAG TGAGAGGAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGGC TCTAGCTATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGACTT TTGCAGAGAC GGCCCAAATT CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGCC AAGCTG
