NM_198007.2;ASC1p200; B630009I04Rik; D430001L07Rik;D630041L21; Helic1; RNAH
100μg liquid plasmid(Instantly):$163
| name | pLV3-CMV-Ascc3(mouse)-3×FLAG-CopGFP-Puro | another name | apLV3-CMV-Ascc3(mouse)-3×FLAG-CopGFP-Puro |
| Insert Size (bp) | 6594bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | pLV3-CMV-MCS-3×FLAG-CopGFP-Puro | 5′ sequencing primer | CMV-F |
| 3′ sequencing primer | EF1a-R | Copy number | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT TATCGATACT AGTATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTTT ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGATTC TAGAGCTAGC CTGATACGAA CTCGGAATTC GCCACCATGG CTCTACCTCG GCTCACAGGA GCCTTGCGCT CCTTTTCAAA TGTCACCAAG CAAGATAATT ACAATGAAGA AGTAGCTGAC TTGAAGTTGA AGCGATCTAA ACTTCATGAA CAAGTTTTAG ATTTTGGCCT TACATGGAAG AAGATAGTGA AATTTTTGAA TGAAAAGCTA GAGAAGAATA AAATGCAAAA TATAAATGAA GACTTAAAAG ACATACTACA AGCTGCAAAA CAAATCGTTG GAACGGATAA TGGGAGAGAA GCAATTGAGA GTGGGGCTGC ATTTCTCTTT ATGACATTTC ACATGACGGA CTCTGTTGGT TACATGGAGA CAAAGGCTAT CAGACAGACG TTTGGTCCCT TTCCATCGTC ATCTGCTACT TCAGCTTGTA ATGCCACTAA TCGAATTATT TCTCATTTTA GTCAAGATGA TCTCACTGCT TTTGTCCAGA TGGCAGAAAA CCCATGTAAC GATAGAGTGG TTTTTGGCAA AAACTTAGCA TTTTCTTTTG ACATGTATGA TCTGGACCAC TTTGATGAAT TACCAATAAA TGGTGAATCC CAGAAAACTA TAAGCTTAGA CTACAAGAAG TTCCTGAATG AACAGTTCCA GGAACCCTAC ACTCCTGAGC TCAAGCCTGT GGAGAAAACA AATGGCTCCC TATTGTGGTG TGAAGTTGAG AAGTACTTAA ACGCAACGTT AAAGGAAATG ACTGAAGCAG CAAGAGTTGA AGATCTTTGC TGTACTCTAT ATGACATGCT TGCTTCTGCT AAAAGCGGCG ATGAACTTCA GGACGAGCTG TTTGAATTGC TGGGACCTGA AGGACTGGAT CTGATTGAGA AGCTGCTCCA GAACAGAATT ACAATTGTAG ATCGGTTCCT TAATTCCTCG AGCGATCATA AGTTTCAAGT TCTCCAAGAT AGTTGTAAAA AGATTTTAGG AGAGAATTCA AAGCCTAATT ATGGATGTCA AGTCACTATT CAATCTGAAC AAGAAAAACA GTTAATGAAA CAATATCGAC GTGAAGAAAA AAGAATTGCC AGGCGAGAAA AAAAGGCTGG AGAAGATGGG GAAGTTTCTG GAGAAGGAGT TTTGCCCTTT GATCCTAAGG AACTCCGGAT ACAGAGAGAA CATGCACTTC TGAATGCTAG AAATGCTCCA ATTTTAGGAA GACAGAGAGA CGTGGAGTTT GAAAAAATCC GTTATCCTCA TGTTTATGAT TCCCAGGCTC AGGCCCGAGA AACATCAGCA TTCATAGCTG GAGCCAAGAT GATTTTACCA GAAGGAATCC AGAGAGAGAA TACCAAGCTG TATGAGGAAG TCAGGATCCC CTATGGTGAA CCAATGCCAG TTGGGTTTGA GGAAAAACCA GTTTATATCA AAGATTTAGA TGAGGTTGGA CAGTTGGCTT TTAAAGGAAT GAAGAGACTC AATAGAATCC AGTCTATAGT ATTTGAAACA GCCTACAACA CCAACGAAAA CATGCTGATC TGTGCCCCTA CGGGAGCTGG GAAGACCAAT ATTGCAATGC TTACTATCTT ACATGAAATC CGCCAACACT TTCATCAAGG TGTTATTAAA AAGAATGAGT TTAAGATTGT GTATGTCGCT CCAATGAAAG CCTTGGCAGC TGAGATGACA AATTACTTCA GCAAACGTCT AGAGCCACTG GGCATTGTTG TGAAAGAATT GACTGGAGAT ATGCAGTTGT CAAAAAGTGA AATTTTACGA ACTCAGATGC TGGTGACCAC ACCAGAAAAA TGGGATGTAG TAACAAGAAA GAGTGTTGGG GATGTTGCTC TTTCCCAAAT CGTAAAACTC CTGATTCTTG ACGAGGTGCA CTTGCTGCAT GAAGACAGAG GCCCAGTGCT AGAAAGCATA GTCGCCAGGA CCTTACGGCA GGTTGAATCC ACACAGAGTA TGATAAGAAT TCTTGGATTG TCTGCAACCT TACCCAACTA CCTCGATGTT GCTACATTTT TGCATGTTAA CCCCTACATT GGACTTTTCT ACTTTGATGG CCGTTTTCGA CCTGTACCTC TTGGACAGAC ATTTTTAGGG ATTAAAAGTA CAAACAAGAT GCAGCAGTTA AATAACATGG ATGAAGTGTG CTACGAAAGT GTTCTGAAAC AAGTAAAGGC TGGACACCAG GTGATGGTAT TTGTACATGC TCGCAATGCC ACTGTAAGGA CCGCTATGTC TCTAATAGAG AGAGCAAAAA ATAGTGGCCA AATTTCTTGC TTTCTACCCA CCGAAGGGCC AGAGTACGGA CATGCATTAA AACAGGTACA AAAGTCAAGA AATAAGCAAG TGCGAGAATT ATTCTCCGAT GGCTTTAGCA TTCATCATGC CGGGATGCTT CGGCAAGACA GAAATTTGGT TGAAAATTTG TTTTCAAATG GGCATATCAA AGTCCTAGTC TGTACAGCCA CATTAGCCTG GGGTGTCAAT CTTCCTGCAC ATGCCGTGGT TATTAAGGGA ACACAAATAT ACGCTGCCAA AAGAGGCTCC TTTGTTGACC TTGGAATTTT AGATGTCATG CAGATATTTG GCCGAGCTGG ACGACCGCAA TTTGACAAAT TTGGGGAAGG AATCATTATA ACAACCCACG ATAAACTCAG CCATTACCTC AGTTTGCTCA CTCAACAAAA CCCAATTGAG AGTCAGTTTC TGGAAAGTCT TGCAGATAAC CTAAATGCAG AGATTGCGCT GGGGACAGTC ACCAACGTGG AAGAGGCAGT GAGGTGGATG AGTTACACTT ACCTCTATGT GCGCATGCGA GCAAACCCGC TAGCATACGG CATCAGTCAC AAAGCGTACC AGATTGACCC AACACTAAGG AAGCATCGAG AACAGCTCCT TATTGAAGTT GGACAAAAGT TGGATAAAGC AAAGATGATT CGTTTTGAAG AACGAACTGG CTATTTTTCT TCTACTGATT TGGGTAGAAC TGCCAGCCAC TTCTATATTA AATACAACAC CATTGAGACA TTTAATGAAC TCTTTGATGC ACACAAAACA GAGGGTGATA TCTTTGCTAT TGTTTCCAAA GCTGAAGAAT TTGATCAAAT TAAGGTCAGA GAAGAAGAAA TAGAAGAATT AGATGCCTTG TTAAACAATT TCTGTGAACT CTCAGCTCCA GGAGGTGTGG AAAATAGTTA TGGGAAAATA AATATCCTAC TTCAAACCTA CATCAGCCGA GGAGAGATGG ACAGTTTCTC CCTCATATCA GATTCTGCAT ATGTCGCCCA GAATGCAGCT AGAATTGTCC GTGCTCTCTT TGAAATTGCA CTGAGGAAAC GTTGGCCTAC CATGACCTAC AGGCTTTTGA ATCTTAGTAA AGTCATTGAC AAGAGACTCT GGGGTTGGGC TAGCCCTTTG AGACAATTTT CTGTGTTACC ACCACACATT CTAACAAGAT TAGAAGAAAA AAACCTTACT GTGGATAAAC TCAAAGACAT GAGAAAAGAT GAAATAGGTC ACATTTTGCA TCATGTGAAT ATTGGACTGA AGGTCAAACA GTGTGTTCAT CAAATTCCTT CTGTTACAAT GGAAGCTTCC ATTCAGCCCA TCACACGGAC TGTCCTCCGA GTATCACTGA ACATCCACCC TGATTTCTCT TGGAATGACC AGGTTCATGG AACAGTGGGG GAACCATGGT GGATTTGGGT AGAAGATCCT ACAAATGACC ATATTTATCA CTCGGAGTAC TTTCTAGCTC TTAAAAAACA GGTTATTAAT AAGGAAGCTC AACTTCTGGT ATTTACAATT CCTATTTTTG AGCCTTTGCC CTCTCAGTAC TACATTCGAG CAGTGTCTGA TCGATGGCTA GGAGCTGAAG CTGTTTGCAT TATCAACTTT CAACATCTGA TTCTACCAGA GAGACATCCT CCCCATACAG AATTATTGGA CCTTCAACCT TTACCAATTA CAGCTTTGGG GTGCAAAGCA TATGAAGCTC TATATAACTT CAGCCACTTT AACCCTGTAC AGACGCAGAT ATTCCACACA CTGTATCACA CAGACTGTAA TGTTCTGCTT GGCGCCCCCA CCGGATCAGG AAAGACAGTT GCAGCTGAAC TAGCCATTTT CAGAGTTTTC AACAAATATC CTACTTCAAA GGCAGTATAT ATTGCACCCC TCAAAGCTCT AGTACGTGAA AGAATGGATG ATTGGAAAAT TAGGATAGAA GAAAAACTTG GTAAAAAAGT TATTGAACTA ACTGGGGATG TGACTCCAGA CATGAAATCC ATTGCTAAGG CTGACCTTAT TGTCACCACA CCAGAAAAGT GGGATGGAGT TAGTCGAAGC TGGCAGAATA GAAGCTATGT TCAGCAGGTC AACATTCTCA TCATAGATGA GATCCATCTG CTAGGGGAGG AGAGAGGCCC AGTTTTAGAA GTCATTGTAT CTCGAACAAA CTTTATCTCA TCACACACAG AAAAACCAGT TAGAATAGTT GGACTGTCCA CTGCATTAGC TAATGCTAGA GATCTTGCTG ACTGGCTCAA CATTAAGCAG ATGGGTTTGT TTAACTTCCG ACCATCAGTT CGCCCAGTTC CACTGGAAGT TCACATTCAG GGTTTCCCAG GACAACATTA CTGCCCACGC ATGGCTAGTA TGAACAAGCC TGCATTTCAG GCAATTAGAA GTCACTCTCC AGCCAAGCCT GTTTTGATAT TTGTCTCATC AAGACGTCAA ACACGACTTA CAGCCTTAGA GTTGATAGCA TTTCTGGCCA CTGAAGAAGA CCCAAAGCAG TGGTTGAATA TGGATGAGCA AGAGATGGAC AACATCATTG GAACAGTACG AGATTCCAAC CTCAAGCTGA CTCTAGCTTT TGGAATAGGA ATGCATCATG CTGGCCTGCA TGAAAGAGAC CGAAAAACTG TAGAAGAGCT GTTTGTGAAC TGTAAAGTTC AGGTTCTCAT TGCTACAAGC ACATTAGCAT GGGGTGTAAA CTTTCCAGCA CACTTAGTAA TTATTAAGGG AACTGAATAT TATGATGGAA AGACAAGACG TTATGTGGAT TTTCCCATCA CAGATGTGCT GCAGATGATG GGACGAGCTG GGAGACCTCA GTTTGATGAC CAGGGCAAAG CTGTAATTCT AGTTCATGAT ATAAAAAAAG ATTTTTATAA AAAGTTTCTT TATGAACCTT TCCCAGTAGA ATCAAGCTTA TTAGGAGTGT TGTCTGACCA CTTAAATGCA GAGATTGCTG GAGGTACAAT AACATCAAAG CAAGATGCAA TGGATTATAT CACCTGGACT TACTTTTTCC GGCGTCTTAT CATGAATCCC AGCTACTACA GCTTGGGTGA CGTCAGCCAG GACTCCATAA ACAAGTTCCT GTCACATCTG ATTGGGCAGT CTCTGGTTGA ATTGGAGCTT TCCCACTGCA TTGAAGTTGG AGAGGATAAT CGCACCATTG AACCACTGAC ATGTGGTCGA ATTGCCTCCT ATTACTATTT GAAGCACAAA ACTGTCAAAA TGTTCAAGGA CCGTTTGAAA CCTGAGTGCA GCACTGAGGA ACTGCTGTCA ATTCTGAGTG ATGCAGAAGA ATACACAGAC TTACCAGTAA GACACAATGA GGACCATACG AATAATGAAT TGGCCAAATG TCTTCCCATT GAATTAAATC CTCATTCATT TGACAGCCCT CACACCAAAG CCCATCTCTT GCTTCAAGCG CATCTCAGCC GAGCCATGCT GCCCTGCCCA GATTATGATA CTGATACCAA AACAGTCTTA GACCAAGCTC TCAGAGTATG CCAGGCAATG CTGGATGTGG CTGCAAGCCA GGGATGGCTA GTGACCGTCC TGAATATCAC CCACCTGATT CAGATGGTGA TCCAAGGCCG ATGGCTCAAG GACTCTTCTC TTCTCACAAT ACCAAACATA GAGCAGCACC ACCTTCACCT TTTCAGGAAA TGGAAGCCAC CTGTAAAGAG CTCACATGCT AAGTGCCGAA CATCCATTGA GTGCCTTCCT GAGCTGATCC ATGCCTGTGA AGGGAAGGAC CATGTGTTCA GCTCCATGGT AGAGAAAGAG TTACAGCCTG CAAAAACAAA GCAGGCATGG AATTTCTTAT CTCGCTTGCC AGTGATAAAT GTTGGCATAA GTGTTAAAGG CTCATGGGAT GACTTAGTTG AAGGACATAA TGAACTCTCC ATCTCAACTC TGACAGCAGA CAAACGGGAT GAAAACAAAT GGATCAAGTT GCATGCTGAT CAGGAGTATG TGCTTCAAGT CAGCCTACAG AGAGTCCATT TTGGGCTTCC AAAGGGAAAA CATGAGAATC ACGCAGTTAC TCCTCGATTT CCAAAATTAA AAGATGAAGG CTGGTTTTTG ATATTAGGAG AAGTGGATAA GAGAGAGCTG ATGGCTGTAA AACGAGTAGG ATTTGTTCGA ACACATCATG ATGCTTCCAT CTCGTTCTTT ACTCCTGAAA CACCTGGAAG ATATATCTTT ACCCTGTATC TCATGAGTGA CTGTTACCTC GGCCTAGACC AGCAGTATGA CATTTATCTC AATGTCATAA AAGCAAACAT TTCTACAAAG GACTCTGATG TCTTCACTGA CTTGTCAGTA GGATCCGACT ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA CTACAAGGAT GACGATGACA AGTAGCGGCC GCGAAGGATC TGCGATCGCT CCGGTGCCCG TCAGTGGGCA GAGCGCACAT CGCCCACAGT CCCCGAGAAG TTGGGGGGAG GGGTCGGCAA TTGAACGGGT GCCTAGAGAA GGTGGCGCGG GGTAAACTGG GAAAGTGATG TCGTGTACTG GCTCCGCCTT TTTCCCGAGG GTGGGGGAGA ACCGTATATA AGTGCAGTAG TCGCCGTGAA CGTTCTTTTT CGCAACGGGT TTGCCGCCAG AACACAGCTG AAGCTTCGAG GGGCTCGCAT CTCTCCTTCA CGCGCCCGCC GCCCTACCTG AGGCCGCCAT CCACGCCGGT TGAGTCGCGT TCTGCCGCCT CCCGCCTGTG GTGCCTCCTG AACTGCGTCC GCCGTCTAGG TAAGTTTAAA GCTCAGGTCG AGACCGGGCC TTTGTCCGGC GCTCCCTTGG AGCCTACCTA GACTCAGCCG GCTCTCCACG CTTTGCCTGA CCCTGCTTGC TCAACTCTAC GTCTTTGTTT CGTTTTCTGT TCTGCGCCGT TACAGATCCA AGCTGTGACC GGCGCCTACG CTAGACGCCA CCATGGAGAG CGACGAGAGC GGCCTGCCCG CCATGGAGAT CGAGTGCCGC ATCACCGGCA CCCTGAACGG CGTGGAGTTC GAGCTGGTGG GCGGCGGAGA GGGCACCCCC AAGCAGGGCC GCATGACCAA CAAGATGAAG AGCACCAAAG GCGCCCTGAC CTTCAGCCCC TACCTGCTGA GCCACGTGAT GGGCTACGGC TTCTACCACT TCGGCACCTA CCCCAGCGGC TACGAGAACC CCTTCCTGCA CGCCATCAAC AACGGCGGCT ACACCAACAC CCGCATCGAG AAGTACGAGG ACGGCGGCGT GCTGCACGTG AGCTTCAGCT ACCGCTACGA GGCCGGCCGC GTGATCGGCG ACTTCAAGGT GGTGGGCACC GGCTTCCCCG AGGACAGCGT GATCTTCACC GACAAGATCA TCCGCAGCAA CGCCACCGTG GAGCACCTGC ACCCCATGGG CGATAACGTG CTGGTGGGCA GCTTCGCCCG CACCTTCAGC CTGCGCGACG GCGGCTACTA CAGCTTCGTG GTGGACAGCC ACATGCACTT CAAGAGCGCC ATCCACCCCA GCATCCTGCA GAACGGGGGC CCCATGTTCG CCTTCCGCCG CGTGGAGGAG CTGCACAGCA ACACCGAGCT GGGCATCGTG GAGTACCAGC ACGCCTTCAA GACCCCCATC GCCTTCGCCA GATCCCGCGC TCAGTCGTCC AATTCTGCCG TGGACGGCAC CGCCGGACCC GGCTCCACCG GATCTCGCGA GGGCAGAGGA AGTCTTCTAA CATGCGGTGA CGTGGAGGAG AATCCCGGCC CTATGACCGA GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG TACGCACCCT CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG ACCGCCACAT CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA GGTGCCCGAA GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GAAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAATC ATCGTCCTTT CCTTGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGGC CGCCTCCCCG CCTGGTACCT TTAAGACCAA TGACTTACAA GGCAGCTGTA GATCTTAGCC ACTTTTTAAA AGAAAAGGGG GGACTGGAAG GGCTAATTCA CTCCCAACGA AGATAAGATC TGCTTTTTGC TTGTACTGGG TCTCTCTGGT TAGACCAGAT CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC CCTCAGACCC TTTTAGTCAG TGTGGAAAAT CTCTAGCAGT AGTAGTTCAT GTCATCTTAT TATTCAGTAT TTATAACTTG CAAAGAAATG AATATCAGAG AGTGAGAGGA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG GCTCTAGCTA TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGA CTTTTGCAGA GACCAAATTC GTAATCATGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGCCAA GCTG
