NM_005691.4；ABC37; ATFB12; CANTU; CMD1O; IDMYS; SUR2
100μg liquid plasmid(Instantly):$134
| name | PiggyBac-CMV-ABCC9(human-Toxic-opt)-Neo | another name | aPiggyBac-CMV-ABCC9(human-Toxic-opt)-Neo |
| Insert Size (bp) | 4647bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression,Transpose | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | PiggyBac-CMV-MCS-EF1a-Neo | 5′ sequencing primer | CMV-F |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAGCGC GCCTCGTTCA TTCACGTTTT TGAACCCGTG GAGGACGGGC AGACTCGCGG TGCAAATGTG TTTTACAGCG TGATGGAGCA GATGAAGATG CTCGACACGC TGCAGAACAC GCAGCTAGAT TAACCCTAGA AAGATAATCA TATTGTGACG TACGTTAAAG ATAATCATGC GTAAAATTGA CGCATGTGTT TTATCGGTCT GTATATCGAG GTTTATTTAT TAATTTGAAT AGATATTAAG TTTTATTATA TTTACACTTA CATACTAATA ATAAATTCAA CAAACAATTT ATTTATGTTT ATTTATTTAT TAAAAAAAAA CAAAAACTCA AAATTTCTTC TATAAAGTAA CAAAACTTTT ATGAGGGACA GCCCCCCCCC AAAGCCCCCA GGGATGTAAT TACGTCCCTC CCCCGCTAGG GGGCAGCAGC GAGCCGCCCG GGGCTCCGCT CCGGTCCGGC GCTCCCCCCG CATCCCCGAG CCGGCAGCGT GCGGGGACAG CCCGGGCACG GGGAAGGTGG CACGGGATCG CTTTCCTCTG AACGCTTCTC GCTGCTCTTT GAGCCTGCAG ACACCTGGGG GGATACGGGG AAAAGGCCTC CAAGGCCTAC TAGTATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCGCCT GGAGACGCCA TCCACGCTGT TTTGACCTCC ATAGAAGATT CTAGAGCTAG CGAATTCGCC ACCATGAGCC TTTCATTTTG TGGTAACAAC ATTTCTTCAT ATAATATCAA CGATGGTGTA CTACAAAATT CCTGCTTTGT GGATGCCCTC AACCTGGTCC CTCATGTCTT TCTGTTGTTT ATCACTTTTC CAATATTGTT TATTGGGTGG GGGAGCCAAA GCTCAAAAGT ACAAATTCAC CACAACACAT GGCTTCATTT TCCGGGACAT AACCTGAGAT GGATTCTTAC ATTCGCTCTC CTGTTTGTGC ATGTCTGTGA AATAGCAGAA GGCATTGTTT CAGACTCGCG GCGGGAATCA AGGCACCTCC ACCTCTTTAT GCCAGCCGTG ATGGGATTCG TTGCCACTAC AACATCGATA GTGTATTATC ATAATATCGA AACATCAAAT TTTCCTAAAT TACTTTTAGC CCTGTTCCTG TATTGGGTAA TGGCCTTTAT TACAAAAACA ATAAAATTGG TTAAGTACTG TCAGTCTGGC TTGGACATAT CAAACCTGCG TTTCTGCATC ACAGGCATGA TGGTCATCTT GAATGGGCTC TTGATGGCTG TGGAGATCAA TGTCATTCGA GTCAGGAGAT ATGTATTTTT CATGAATCCT CAGAAAGTAA AGCCTCCTGA AGACCTCCAG GATCTGGGAG TGAGATTTCT TCAACCATTT GTGAATTTGC TGTCAAAAGC AACATACTGG TGGATGAACA CACTTATTAT ATCTGCTCAC AAAAAGCCTA TTGATCTGAA GGCAATTGGA AAATTGCCAA TAGCAATGAG AGCAGTAACA AATTATGTTT GCCTGAAAGA TGCATATGAA GAACAAAAGA AAAAAGTTGC AGATCATCCA AATCGGACTC CATCTATATG GCTTGCAATG TACAGAGCTT TTGGGCGACC AATTCTACTT AGTAGCACAT TCCGCTATCT GGCTGATTTA CTGGGTTTTG CTGGACCTCT TTGTATTTCT GGAATAGTTC AGCGTGTGAA TGAAACCCAG AATGGGACAA ATAACACAAC TGGAATTTCA GAAACCCTCT CATCAAAGGA ATTTCTTGAA AACGCTTACG TTCTAGCAGT TCTTCTCTTC TTGGCTCTTA TTCTGCAAAG GACATTTTTG CAGGCTTCCT ACTATGTAAC CATAGAGACT GGCATTAACC TCCGTGGAGC TCTGCTGGCC ATGATTTATA ATAAAATCCT TAGGCTCTCT ACGTCTAACT TATCCATGGG GGAGATGACT CTGGGGCAGA TCAACAACTT AGTCGCCATT GAAACTAATC AACTCATGTG GTTTTTGTTC CTGTGTCCCA ATCTATGGGC TATGCCTGTT CAGATCATAA TGGGCGTAAT CCTCTTGTAC AACCTGCTCG GATCCTCTGC TCTCGTGGGA GCTGCAGTCA TCGTTCTTCT TGCTCCCATC CAATATTTCA TCGCAACCAA ACTTGCGGAG GCCCAGAAGA GCACTCTGGA TTACTCCACG GAAAGACTCA AGAAGACCAA TGAGATCCTG AAGGGTATCA AGCTGCTGAA ACTGTATGCC TGGGAGCATA TTTTCTGTAA GTCTGTCGAA GAAACACGGA TGAAGGAGCT GTCCAGCCTG AAAACCTTTG CCCTGTACAC TAGCTTGAGT ATCTTCATGA ATGCCGCCAT TCCGATAGCA GCAGTGCTCG CTACCTTTGT GACGCATGCA TACGCTTCCG GTAACAACCT GAAACCCGCT GAAGCCTTTG CCAGTCTTAG CCTGTTCCAT ATTTTGGTGA CGCCCTTGTT TCTGCTGTCA ACGGTGGTTA GGTTTGCTGT GAAAGCCATT ATATCCGTGC AGAAGCTGAA TGAGTTTCTG CTTTCAGACG AAATTGGCGA CGATAGCTGG AGAACCGGTG AAAGTTCACT GCCTTTTGAA TCATGCAAAA AACACACTGG CGTCCAGCCT AAGACAATTA ACAGGAAACA GCCTGGGCGA TATCACCTGG ATTCATACGA GCAATCCACA CGACGCCTCC GACCAGCAGA AACAGAGGAT ATCGCTATCA AGGTGACTAA CGGATACTTT AGCTGGGGCT CTGGGCTGGC AACACTGTCC AACATTGATA TCCGCATTCC GACCGGCCAA CTCACCATGA TCGTTGGGCA GGTTGGCTGC GGCAAATCAT CTCTGCTTCT GGCTATTCTG GGCGAAATGC AGACTCTCGA GGGTAAGGTT CACTGGAGCA ATGTGAACGA AAGCGAGCCT TCCTTCGAAG CCACTAGATC AAGAAATCGC TACAGTGTCG CTTATGCTGC ACAGAAACCA TGGCTCCTGA ACGCGACGGT CGAGGAGAAT ATCACCTTCG GCAGCCCCTT TAACAAGCAG CGCTACAAGG CCGTGACTGA CGCATGCTCC TTGCAGCCCG ACATTGACCT TCTCCCTTTT GGGGATCAAA CTGAAATTGG AGAGCGCGGA ATCAATCTGT CAGGCGGACA ACGGCAAAGA ATCTGTGTGG CACGAGCTTT GTACCAGAAC ACCAATATAG TCTTTCTGGA TGATCCCTTT TCCGCACTTG ACATCCATTT GAGCGACCAT CTGATGCAGG AAGGTATTCT CAAGTTCCTC CAAGATGACA AGCGGACATT GGTCTTGGTC ACACATAAAC TCCAGTACTT GACGCATGCA GACTGGATTA TCGCAATGAA AGACGGAAGC GTTCTTCGCG AAGGGACTCT CAAAGATATC CAAACCAAAG ACGTTGAGCT TTACGAGCAC TGGAAAACCT TGATGAATCG GCAAGATCAA GAATTAGAAA AGGATATGGA AGCTGACCAA ACTACTTTAG AGAGGAAAAC TCTCCGACGG GCCATGTATT CAAGAGAAGC CAAAGCCCAG ATGGAGGACG AAGACGAAGA GGAAGAAGAG GAGGAAGATG AGGATGATAA CATGTCCACT GTAATGAGGC TCAGGACTAA AATGCCATGG AAAACCTGCT GGCGCTACCT GACATCTGGA GGATTCTTCC TGCTCATCCT GATGATTTTC TCTAAGCTTT TGAAGCATTC GGTCATTGTA GCTATAGACT ATTGGCTGGC CACATGGACA TCGGAGTACA GTATAAACAA TACTGGAAAA GCTGATCAGA CCTACTATGT GGCTGGCTTT AGCATACTCT GTGGAGCAGG CATTTTCCTT TGCCTTGTTA CATCCCTCAC TGTAGAATGG ATGGGTCTCA CAGCTGCCAA AAATCTTCAC CACAACCTTC TCAATAAGAT AATCCTTGGA CCAATAAGGT TTTTTGATAC CACACCCCTG GGACTGATTC TCAATCGCTT TTCAGCTGAT ACTAATATCA TTGATCAGCA CATCCCTCCA ACCTTGGAAT CTCTAACTCG CTCAACACTG CTCTGCCTGT CTGCCATTGG GATGATTTCT TATGCTACTC CTGTGTTCCT GGTTGCTCTC CTGCCCCTTG GTGTTGCCTT TTATTTTATC CAGAAATACT TTCGGGTTGC CTCTAAGGAC CTCCAGGAAC TTGACGATAG TACCCAGCTC CCTCTGCTCT GTCACTTCTC AGAAACAGCA GAAGGACTCA CCACCATTCG GGCCTTTAGG CATGAAACCA GATTTAAACA ACGTATGCTG GAACTGACGG ATACAAACAA CATTGCCTAC TTATTTCTCT CAGCTGCCAA CAGATGGCTG GAGGTCAGGA CGGATTATCT GGGAGCTTGC ATTGTCCTCA CTGCATCTAT AGCATCCATT AGTGGGTCTT CGAATTCTGG ATTGGTAGGC TTGGGTCTTC TGTATGCACT TACGATAACC AATTATTTGA ATTGGGTTGT GAGGAACTTG GCTGACCTGG AGGTCCAGAT GGGTGCAGTG AAGAAGGTGA ACAGTTTCCT GACTATGGAG TCAGAGAACT ATGAAGGCAC AATGGATCCT TCTCAAGTTC CAGAACATTG GCCACAAGAA GGGGAGATCA AGATACATGA TCTGTGTGTC AGATATGAAA ATAATCTGAA ACCTGTTCTT AAGCACGTCA AGGCTTACAT CAAACCTGGA CAAAAGGTGG GCATATGTGG TCGCACTGGC AGTGGGAAAT CATCGTTATC TCTGGCTTTC TTCAGAATGG TTGATATATT TGATGGAAAA ATTGTCATTG ATGGGATAGA CATTTCCAAA TTACCACTGC ACACACTACG TTCTAGACTT TCAATCATTC TGCAGGATCC AATACTATTC AGTGGTTCCA TTAGATTTAA TTTAGATCCA GAGTGCAAAT GCACAGATGA CAGACTCTGG GAAGCCTTAG AAATTGCTCA GCTGAAGAAT ATGGTCAAAT CTCTACCTGG AGGTCTAGAT GCGGTTGTCA CTGAAGGTGG GGAGAATTTT AGCGTTGGAC AGAGACAGCT ATTTTGCCTT GCCAGGGCCT TTGTCCGCAA AAGCAGCATT CTTATTATGG ATGAGGCAAC AGCTTCCATT GACATGGCCA CAGAGAATAT TTTGCAAAAA GTAGTAATGA CAGCCTTTGC AGACCGGACC GTGGTGACAA TAGCTCACCG TGTCTCTTCT ATTATGGATG CAGGCCTTGT TTTAGTCTTT TCTGAGGGTA TTTTAGTGGA GTGTGATACT GTCCCAAATT TGCTCGCCCA CAAGAATGGC CTCTTTTCCA CTTTGGTGAT GACCAACAAG GCGGCCGCGA AGGATCTGCG ATCGCTCCGG TGCCCGTCAG TGGGCAGAGC GCACATCGCC CACAGTCCCC GAGAAGTTGG GGGGAGGGGT CGGCAATTGA ACGGGTGCCT AGAGAAGGTG GCGCGGGGTA AACTGGGAAA GTGATGTCGT GTACTGGCTC CGCCTTTTTC CCGAGGGTGG GGGAGAACCG TATATAAGTG CAGTAGTCGC CGTGAACGTT CTTTTTCGCA ACGGGTTTGC CGCCAGAACA CAGCTGAAGC TTCGAGGGGC TCGCATCTCT CCTTCACGCG CCCGCCGCCC TACCTGAGGC CGCCATCCAC GCCGGTTGAG TCGCGTTCTG CCGCCTCCCG CCTGTGGTGC CTCCTGAACT GCGTCCGCCG TCTAGGTAAG TTTAAAGCTC AGGTCGAGAC CGGGCCTTTG TCCGGCGCTC CCTTGGAGCC TACCTAGACT CAGCCGGCTC TCCACGCTTT GCCTGACCCT GCTTGCTCAA CTCTACGTCT TTGTTTCGTT TTCTGTTCTG CGCCGTTACA GATCCAAGCT GTGACCGGCG CCTACGCTAG GCCACCATGG CTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGTCGACAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCGTTAAC TAAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGGAATTGA CTCAAATGAT GTCAATTAGT CTATCAGAAG CTATCTGGTC TCCCTTCCGG GGGACAAGAC ATCCCTGTTT AATATTTAAA CAGCAGTGTT CCCAAACTGG GTTCTTATAT CCCTTGCTCT GGTCAACCAG GTTGCAGGGT TTCCTGTCCT CACAGGAACG AAGTCCCTAA AGAAACAGTG GCAGCCAGGT TTAGCCCCGG AATTGACTGG ATTCCTTTTT TAGGGCCCAT TGGTATGGCT TTTTCCCCGT ATCCCCCCAG GTGTCTGCAG GCTCAAAGAG CAGCGAGAAG CGTTCAGAGG AAAGCGATCC CGTGCCACCT TCCCCGTGCC CGGGCTGTCC CCGCACGCTG CCGGCTCGGG GATGCGGGGG GAGCGCCGGA CCGGAGCGGA GCCCCGGGCG GCTCGCTGCT GCCCCCTAGC GGGGGAGGGA CGTAATTACA TCCCTGGGGG CTTTGGGGGG GGGCTGTCCC TGATATCTAT AACAAGAAAA TATATATATA ATAAGTTATC ACGTAAGTAG AACATGAAAT AACAATATAA TTATCGTATG AGTTAAATCT TAAAAGTCAC GTAAAAGATA ATCATGCGTC ATTTTGACTC ACGCGGTCGT TATAGTTCAA AATCAGTGAC ACTTACCGCA TTGACAAGCA CGCCTCACGG GAGCTCCAAG CGGCGACTGA GATGTCCTAA ATGCACAGCG ACGGATTCGC GCTATTTAGA AAGAGAGAGC AATATTTCAA GAATGCATGC GTCAATTTTA CGCAGACTAT CTTTCTAGGG TTAATCTAGC TGCATCAGGA TCATATCGTC GGGTCTTTTT TCCGGCTCAG TCATCGCCCA AGCTGGCGCT ATCTGGGCAT CGGGGAGGAA GAAGCCCGTG CCTTTTCCCG CGAGGTTGAA GCGGCATGGA AAGAGTTTGC CGAGGATGAC TGCTGCTGCA TTGACGTTGA GCGAAAACGC ACGTTTACCA TGATGATTCG GGAAGGTGTG GCCATGCACG CCTTTAACGG TGAACTGTTC GTTCAGGCCA CCTGGGATAC CAGTTCGTCG CGGCTTTTCC GGACACAGTT CCGGATGGTC AGCCCGAAGC GCATCAGCAA CCCGAACAAT ACCGGCGACA GCCGGAACTG CCGTGCCGGT GTGCAGATTA ATGACAGCGG TGCGGCGCTG GGATATTACG TCAGCGAGGA CGGGTATCCT GGCTGGATGC CGCAGAAATG GACATGGATA CCCCGTGAGT TACCCGGCGG GCGCGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC AT
