NM_008737.3;C530029I03; NP-1; NPN-1; Npn1; Nrp;
100μg liquid plasmid(Instantly):$105
| name | pDC316-CMV-Nrp1(mouse)-MBH-mNeonGreen | another name | apDC316-CMV-Nrp1(mouse)-MBH-mNeonGreen |
| Insert Size (bp) | 2772bp | Plasmid host | Mammalian cell,Adenovirus |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | pDC316-MBH-mNeonGreen-CMV-MCS | 5′ sequencing primer | CMV-F |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TTCATCAATA ATATACCTTA TTTTGGATTG AAGCCAATAT GATAATGAGG GGGTGGAGTT TGTGACGTGG CGCGGGGCGT GGGAACGGGG CGGGTGACGT AGTAGTGTGG CGGAAGTGTG ATGTTGCAAG TGTGGCGGAA CACATGTAAG CGACGGATGT GGCAAAAGTG ACGTTTTTGG TGTGCGCCGG TGTACACAGG AAGTGACAAT TTTCGCGCGG TTTTAGGCGG ATGTTGTAGT AAATTTGGGC GTAACCGAGT AAGATTTGGC CATTTTCGCG GGAAAACTGA ATAAGAGGAA GTGAAATCTG AATAATTTTG TGTTACTCAT AGCGCGTAAT ATTTGTCTAG GGCCGCGGGG ACTTTGACCG TTTACGTGGA GACTCGCCCA GGTGTTTTTC TCAGGTGTTT TCCGCGTTCC GGGTCAAAGT TGGCGTTTTA TTATTATAGT CAGTCTAGAG ATATACTGAG TCATTAGGGA CTTTCCAATG GGTTTTGCCC AGTACATAAG GTCAATAGGG GTGAATCAAC AGGAAAGTCC CATTGGAGCC AAGTACACTG AGTCAATAGG GACTTTCCAT TGGGTTTTGC CCAGTACAAA AGGTCAATAG GGGGTGAGTC AATGGGTTTT TCCCATTATT GGCACGTACA TAAGGTCAAT AGGGGTGAGT CATTGGGTTT TTCCAGCCAA TTTAATTAAA ACGCCATGTA CTTTCCCACC ATTGACGTCA ATGGGCTATT GAAACTAATG CAACGTGACC TTTAAACGGT ACTTTCCCAT AGCTGATTAA TGGGAAAGTA CCGTTCTCGA GCCAATACAC GTCAATGGGA AGTGAAAGGG CAGCCAAAAC GTAACACCGC CCCGGTTTTC CCCTGGAAAT TCCATATTGG CACGCATTCT ATTGGCTGAG CTGCGTTCTA CGTGGGTATA AGAGGCGCGA CCAGCGTCGG TACCGTCGCA GTCTTCGGTC TGACCACCGT AGAACGCAGA TCGAATTCAT GGTGAGCAAG GGCGAGGAGG ATAACATGGC CTCTCTCCCA GCGACACATG AGTTACACAT CTTTGGCTCC ATCAACGGTG TGGACTTTGA CATGGTGGGT CAGGGCACCG GCAATCCAAA TGATGGTTAT GAGGAGTTAA ACCTGAAGTC CACCAAGGGT GACCTCCAGT TCTCCCCCTG GATTCTGGTC CCTCATATCG GGTATGGCTT CCATCAGTAC CTGCCCTACC CTGACGGGAT GTCGCCTTTC CAGGCCGCCA TGGTAGATGG CTCCGGATAC CAAGTCCATC GCACAATGCA GTTTGAAGAT GGTGCCTCCC TTACTGTTAA CTACCGCTAC ACCTACGAGG GAAGCCACAT CAAAGGAGAG GCCCAGGTGA AGGGGACTGG TTTCCCTGCT GACGGTCCTG TGATGACCAA CTCGCTGACC GCTGCGGACT GGTGCAGGTC GAAGAAGACT TACCCCAACG ACAAAACCAT CATCAGTACC TTTAAGTGGA GTTACACCAC TGGAAATGGC AAGCGCTACC GGAGCACTGC GCGGACCACC TACACCTTTG CCAAGCCAAT GGCGGCTAAC TATCTGAAGA ACCAGCCGAT GTACGTGTTC CGTAAGACGG AGCTCAAGCA CTCCAAGACC GAGCTCAACT TCAAGGAGTG GCAAAAGGCC TTTACCGATG TGATGGGCAT GGACGAGCTG TACAAGTGAT CATAATCAGC CATACCACAT TTGTAGAGGT TTTACTTGCT TTAAAAAACC TCCCACACCT CCCCCTGAAC CTGAAACATA AAATGAATGC AATTGTTGTT GTTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTAACGCG TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTATG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACATCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAC AACTCCGCCC CATTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT CACTATAGGG AGACCCAAGC TGGCTAGCGG CCACCATGGA GAGGGGGCTG CCGTTGCTGT GCGCCACGCT CGCCCTTGCC CTCGCCCTGG CGGGCGCTTT CCGCAGCGAC AAATGTGGCG GGACCATAAA AATCGAAAAC CCAGGGTACC TCACATCTCC CGGTTACCCT CATTCTTACC ATCCAAGTGA GAAGTGTGAA TGGCTAATCC AAGCTCCGGA ACCCTACCAG AGAATCATGA TCAACTTCAA CCCACATTTC GATTTGGAGG ACAGAGACTG CAAGTATGAC TACGTGGAAG TAATCGATGG GGAGAATGAA GGCGGCCGCC TGTGGGGGAA GTTCTGTGGG AAGATTGCAC CTTCTCCTGT GGTGTCTTCA GGGCCCTTTC TCTTCATCAA ATTTGTCTCT GACTATGAGA CACATGGGGC AGGGTTTTCC ATCCGCTATG AAATCTTCAA GAGAGGGCCC GAATGTTCTC AGAACTATAC AGCACCTACT GGAGTGATAA AGTCCCCTGG GTTCCCTGAA AAATACCCCA ACAGCTTGGA GTGCACCTAC ATCATCTTTG CACCAAAGAT GTCTGAGATA ATCCTGGAGT TTGAAAGTTT TGACCTGGAG CAAGACTCGA ATCCTCCCGG AGGAATGTTC TGTCGCTATG ACCGGCTGGA GATCTGGGAT GGATTCCCTG AAGTTGGCCC TCACATTGGG CGTTATTGTG GGCAGAAAAC TCCTGGCCGG ATCCGCTCCT CTTCAGGCGT TCTATCCATG GTCTTTTACA CTGACAGCGC AATAGCAAAA GAAGGTTTCT CAGCCAACTA CAGTGTGCTA CAGAGCAGCA TCTCTGAAGA TTTTAAGTGT ATGGAGGCTC TGGGCATGGA ATCTGGAGAG ATCCATTCTG ATCAGATCAC TGCATCTTCA CAGTATGGTA CCAACTGGTC TGTAGAGCGC TCCCGCCTGA ACTACCCTGA AAATGGGTGG ACTCCAGGAG AAGACTCCTA CAAGGAGTGG ATCCAGGTGG ACTTGGGCCT CCTGCGATTC GTTACTGCTG TAGGGACACA GGGTGCCATT TCCAAGGAAA CCAAGAAGAA ATATTATGTC AAGACTTACA GAGTAGACAT CAGCTCCAAC GGAGAGGACT GGATCTCCCT GAAAGAGGGA AATAAAGCCA TTATCTTTCA GGGAAACACC AACCCCACAG ATGTTGTCTT AGGAGTTTTC TCCAAACCAC TGATAACTCG ATTTGTCCGA ATCAAACCTG TATCCTGGGA AACTGGTATA TCTATGAGAT TTGAAGTTTA TGGCTGCAAG ATAACAGATT ATCCTTGCTC TGGAATGTTG GGCATGGTGT CTGGACTTAT TTCAGACTCC CAGATTACAG CATCCAATCA AGCCGACAGG AATTGGATGC CAGAAAACAT CCGTCTGGTG ACCAGTCGTA CCGGCTGGGC ACTGCCACCC TCACCCCACC CATACACCAA TGAATGGCTC CAAGTGGACC TGGGAGATGA GAAGATAGTA AGAGGTGTCA TCATTCAGGG TGGGAAGCAC CGAGAAAACA AGGTGTTCAT GAGGAAGTTC AAGATCGCCT ATAGTAACAA TGGCTCTGAC TGGAAAACTA TCATGGATGA CAGCAAGCGC AAGGCTAAGT CGTTCGAAGG CAACAACAAC TATGACACAC CTGAGCTTCG GACGTTTTCA CCTCTCTCCA CAAGGTTCAT CAGGATCTAC CCTGAGAGAG CCACACACAG TGGGCTTGGG CTGAGGATGG AGCTACTGGG CTGTGAAGTG GAAGCACCTA CAGCTGGACC AACCACACCC AATGGGAACC CAGTGGATGA GTGTGACGAC GACCAGGCCA ACTGCCACAG TGGCACAGGT GATGACTTCC AGCTCACAGG AGGCACCACT GTCCTGGCCA CAGAGAAGCC AACCATTATA GACAGCACCA TCCAATCAGA GTTCCCGACA TACGGTTTTA ACTGCGAGTT TGGCTGGGGC TCTCACAAGA CATTCTGCCA CTGGGAGCAT GACAGCCATG CACAGCTCAG GTGGAGTGTG CTGACCAGCA AGACAGGGCC GATTCAGGAC CATACAGGAG ATGGCAACTT CATCTATTCC CAAGCTGATG AAAATCAGAA AGGCAAAGTA GCCCGCCTGG TGAGCCCTGT GGTCTATTCC CAGAGCTCTG CCCACTGTAT GACCTTCTGG TATCACATGT CCGGCTCTCA TGTGGGTACA CTGAGGGTCA AACTACGCTA CCAGAAGCCA GAGGAATATG ATCAACTGGT CTGGATGGTG GTTGGGCACC AAGGAGACCA CTGGAAAGAA GGACGTGTCT TGCTGCACAA ATCTCTGAAA CTATATCAGG TTATTTTTGA AGGTGAAATC GGAAAAGGAA ACCTTGGTGG AATTGCTGTG GATGATATCA GTATTAACAA CCATATTTCT CAGGAAGACT GTGCAAAACC AACAGACCTA GATAAAAAGA ACACAGAAAT TAAAATTGAT GAAACAGGGA GCACTCCAGG ATATGAAGGA GAAGGGGAAG GTGACAAGAA CATCTCCAGG AAGCCAGGCA ATGTGCTTAA GACCCTGGAT CCCATCCTGA TCACCATCAT AGCCATGAGT GCCCTGGGAG TACTCCTGGG TGCAGTCTGT GGAGTTGTGC TGTACTGTGC CTGTTGGCAC AATGGGATGT CAGAAAGGAA CCTATCTGCC CTGGAGAACT ATAACTTTGA ACTTGTGGAT GGTGTAAAGT TGAAAAAAGA TAAACTGAAC CCACAGAGTA ATTACTCAGA GGCGTGATTT AAACGGGCCC TCTAGACTCG AGCGGCCGCC ACTGTGCTGG ATATCTGCAG AATTGATCCG AGCTCGGTAC CAAGCTTAAG TTTAAACCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGGGT CGACTTCGAG CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGGATCGTCT AGCATCGAAG ATCCAATAAC TTCGTATAGC ATACATTATA CGAAGTTATA AGTAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCAATG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAACA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACTC TAGGCAAAAT AGCACCCTCC CGCTCCAGAA CAACATACAG CGCTTCACAG CGGCAGCCTA ACAGTCAGCC TTACCAGTAA AAAAGAAAAC CTATTAAAAA AACACCACTC GACACGGCAC CAGCTCAATC AGTCACAGTG TAAAAAAGGG CCAAGTGCAG AGCGAGTATA TATAGGACTA AAAAATGACG TAACGGTTAA AGTCCACAAA AAACACCCAG AAAACCGCAC GCGAACCTAC GCCCAGAAAC GAAAGCCAAA AAACCCACAA CTTCCTCAAA TCGTCACTTC CGTTTTCCCA CGTTACGTAA CTTCCCATTT TAAGAAAACT ACAATTCCCA ACACATACAA GTTACTCCGC CCTAAAACCT ACGTCACCCG CCCCGTTCCC ACGCCCCGCG CCACGTCACA AACTCCACCC CCTCATTATC ATATTGGCTT CAATCCAAAA TAAGGTATA
