pTol2-ubiquitin-EGFP-nr5a1b(zebrafish)
$70.00
In stock
SKU
PL19848
-
100μg liquid plasmid(Instantly):$91
| name | pTol2-ubiquitin-EGFP-nr5a1b(zebrafish) | another name | apTol2-ubiquitin-EGFP-nr5a1b(zebrafish) |
| Insert Size (bp) | 1509bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression,Transpose | Fragment type | ORF |
| Fragment species | Zebrafish | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | Replicon | ||
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | zubiquitin-GFP-2A-pTol2 | 5′ sequencing primer | pEGFP-C-5' |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCACCTAAAT TGTAAGCGTT AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGA CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACGC TGGCTAAGAA CTCATCAGCC TCCCCGGTCC ATCTACCCAC GTACCAATGC ACCAATTGGC CACAATGACG GCTACTACAT GGTGCCATTC CTTCCTCTTT ATAGGAATGG AGACTACCTC CTGTCCAACA AGGCTCTTGG ATACGAGTAC GCCTACCTGT TGGACCCAGG TCATTGCACA ACACCAGAAA TGCCCTCTGA TCTGCAAAAG ACGTGAATAT CTGTTCAGAC ACCCATATCC ACTCTGTTCC ACACAGGTCA GAGGTTTGTC CAGGAGTTCT TGACAGAGGT GTAAAAAGTA CTCAAAAATT TTACTCAAGT GAAAGTACAA GTACTTAGGG AAAATTTTAC TCAATTAAAA GTAAAAGTAT CTGGCTAGAA TCTTACTTGA GTAAAAGTAA AAAAGTACTC CATTAAAATT GTACTTGAGT ATTAAGGAAG TAAAAGTAAA AGCAAGAAAG AAAACTAGAG ATTCTTGTTT AAGCTTTTAA TCTCAAAAAA CATTAAATGA AATGCATACA AGGTTTTATC CTGCTTTAGA ACTGTTTGTA TTTAATTATC AAACTATAAG ACAGACAATC TAATGCCAGT ACACGCTACT CAAAGTTGTA AAACCTCAGA TTTAACTTCA GTAGAAGCTG ATTCTCAAAA TTGTTAGTGT CAAGCCTAGC TCTTTTGGGG CTGAAAAGCA ATCCTGCAGT GCTGAAAAGC CTCTCACAGG CAGCCGATGC GGGAAGAGGT GTATTAGTCT TGATAGAGAG GCTGCAAATA GCAGGAAACG TGAGCAGAGA CTCCCTGGTG TCTGAAACAC AGGCCAGATG GGCCCTCGAG ACCAGCAAAG TTCTAGAATT TGTCGAAACA TTTATGTTAT ATATTTCCTG AAAAAAATTC TGAGTAAGTT CTTAAGTGTT ATTGCCAGCA ACATAAACAA CAGACGGCAA AATGAATAAA TGATAACAAA GCAGTAGGCT TAAATAAACC TAATTTTTAT AGGCTGTTCT CTACAACCCT CAAACAGTGA TTAGTTTTGT ACTTATAAAC TTGCCCTTTC ATTCATATTT CAAGAAAATT GGTTCAGAAG ATCTGGATAT TCTAGCAGTT GTTCAAGCTC ATGGAGGGAT CAGTGACCTG ATTCCACAAT GACTAGGCCT AATCCAGAAA TTAGATGACT GTCAACATAA AAAGGCACAG CACTCACTAG CTGCCCTATA TATTTTATTA TATTTTACAT ATATTATTTT ATTTATTTAG CTCTGAGTGC TGTACTTTCT GGTTAAAGAA AACTGCTTAC AACAGCTAAC CTGTACTACC TCAGGCTCAG GGAATTTGGA ACAGGTTTGT CTGGTTTGTT TCTTTAACCA TGCATGCTTG TTTTCAACTA TGGCAACACA GTCACATGGG ACATTACAGA AATGATTTGT CGATGACATG CGACTTTTCT TTAATAAAGC GCAAAGATCC CAAAAAGCAA ACTTTTAACA AAAATCATAT AATTATATTT TCAATCCAGC TTTGTAGCAA CTTTGTGCTG CTGTTCACTC AGCAACAGAT AGTCAGTATA AGGTCAGTGT GTCTCAAAGC AGTGCCATCT GTTTCACACA TTGCGTTCTA TATATAAGTG TGCTGGTTGA CACGACACTG TATAAGGCCT AGGCTAAAAC ACAAACAATG TAGAATGACA CTGTGTTTTT TTTGTAAACA AATGTTGTTT TTGGTTAAAC ATCTTTGTGA AAACATCCTC CTGTCATGTA TTTGCTATAT TCAAATGTTA AACCCGTGCA GAATAGAACA TATACAAAAA AAAACAACAC AACACATTTT TAAACATTAT TAAATATCAA GTATTGCTGG CAGTTCTGTT TCTGTTTTAC AGTACCCTTT GCCACAGTTC TCCGCTTTTC CTGGTCCAGA TTCCACAAGT CTGATTCACC AATAGCAAAG CGAATAAACA ACCAAAGCAG CCAATCACTG CTTGTAGACT GTCCTGCGAG ACCGGCCCAT TCCAGCACAT TCTGGAAACT TCCTTTATAT GATAATTATA AATACATTTA AATTATTGAT ACAAAACATG TAATTCCTAG AACATAACCA TAGCAATCAT TAGTTTTCAG GGTAATTATG TATTTTTAGG ATTTGACTGC GGAAAGATCT GGTCATGTGA CGTCTCATGA ACGTCACGGC CCTGGGTTTC TATAAATACA GTAGGACTCT CGACCATCGG CAGATTTTTC GAAGAAGAAG ATCAGTTTCA GGAGCCGTAC TGTTCCGTTT CAACGCAAAT ATTAACGGTA AGAGCGAATT TCCTAGTTTG TTTTCATGCC ATTCTTTAAA ACCATAGCGT ATTACTTTAA TTATAGTAAA CTTTCGCTTT CTTTATTACA AGAGACGTTT TGTGTTGATT CTCCGCGGAC ATTTTCGGTC AGACAATCAG AAAATGACCG CGGAGGACCA GTAACTTGCA TTACACGTAA GTTAAATCTT CGTGTATTAA AATGGTTAGG TTGTTAACGT CAAATAGGTT ACCGTGTTTG CGTGTGATCA GGTTGGTTTT GTTAGATTTT TGTCAGTATT TTTAATTTAT TTGTTTTAGT TTATTTATTT TTTTTGCTGA ATCATAGTTT GTGAACAAAG AACCCGGATG TTACATACAG TACAGCCGCC ATGTTACAGA GAGTTATAAC TTAATCATTT TAAAAATAAT TTTGCCTTAC TTTTAGTTTG TCATGTTGAG AAATGAGGAA ATGTTAAAAT GAGGAAATAT CCAATTAATT TAATATATCA AAATAATCCA TGATTACAAT GCACTGAACT GGAGAAAATT AAGATGTTTT CTAGTGTCAT GAAACAAATG TAAGAGATGT ACATTGTAGA TGTTTTATGT CAAGAATTGG CTAGTTGATG CAGCATACTG GCGATACTCA GTTGTAATAA CAGTAACGTT ACATGTTAAT AGACTACTGA GTATGCTGTT CTGTCTATGT ATGCTCTGTA AGCTACGAGA AGGACTTTTT TAAACAGTAA AGGGTGCAAT ATTTTTACAA ATTGAATTAA ATAAAGGCTG TCTATTAAGT AATATGCTTG ATATTTTTCT TACTTGATCG GAAATAAGAA AAAATATAAA CGTTGTTGCT CTAAAAATCC TAGTTCAGTT TAGCCAACCA CAAATACCTT TTTGTTCCTC CAACAGTTTT TTTTTCTTCT CTATAATATT TGGCAGTCTA TAGTACTCCA AATGTTTCCC CACAGTCTAA CTAATTGGTA CAGCCAAAAT CATGACACTT ATTGCAATAA TAATTTTGGT TCATTGGCAT TGTTGATAGC CTGTGCCACT AATATGGTCG ATTGATCATG CTTCAGGAAG AAAACTATAT TGTTTGATGT AAGATTATTA AATCTTCACC TGCTTCCATT ACAAACTATT CCCATCTTAT TGAATTCTGG TATGTCTTAA AGGATTAGTT CACTTCCCAA ATCAAAATTT ACTTAGCCTT TTTTCATCCA TGATCCCTTT TTTTCATCAT TAATGAAGAA ATTGTTTTTG AAAAAGTTTC AAGATTTTTT TCTCTATATT GTGGAGCTTG TTAGTTTAAA ATTCCAAAAT GCAATATGTG GCTTCAAATG GTTCTAAATG ATCCCAGTCA AGGAATAACA GTCTTATCTA ATGAAACCAT TAGACCTTTA AAAATAAAAA AAATAAAAGT ATTTATTTTT AAATGACTGA GTGATTAAGT TGAATTTCAG CGTTTCCTTA CTGTGTAGAA GTCCTTCCTT ACTGGCCCCA CCCTTTGGTT CTCTGCCAAT CTGCTACCTA ATGTAATGTT GTGGAACATT ATTATTCTTT ATTTCTTAAT TTTTTATTTT TTATTTTAAA ACAATGTAAA CTGCACAGAT GTGCAGTTTG TTTAAAATGG CCAATGCTTT GGAAATGCAT GACATAATTA GATTTCATGA TGCACAAAGC CAAATCTCAG AGCTTGTGCA AAATGAGCTA TCATTTCACT AGGTAAGACC CTAAATTTTC ATATAGGATC ATTTGGACAA TTTTGCTGCA GGTAAAATGC ATTCTATAGT CCACTGTCAG CCATTGTTTT GGATAGTATT TATTTTTCTC TACAAGTATA GTCAATAGTT TTCTATTATT TTAAAGGTTT GTAACATTTA AGGGTGACCA AATGCAAAGT AAAATTTCAT TTTCGGGTGA ACTATCTCGT TTAACATGGG AGAAGTGCAA AACATACATT ATTGGCTAGA ACATTGTAGT ATTTTTTAAA TGGAAATGTG TGATTGCTAA TCTTACTTTG AATTTGTTTA CAGGGATCCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGGGAA GCGGAGCTAC TAACTTCAGC CTGCTGAAGC AGGCTGGAGA CGTGGAGGAG AACCCTGGAC CTCCCGGGAT GTCGACGATG GACTACAGTT ATGATGCGGA CCTGGAAGAG ATGTGTCCGG TGTGCGGGGA TAAAGTTTCT GGCTATCATT ATGGTTTATT AACCTGCGAA AGCTGCAAGG GTTTCTTTAA AAGGACCGTA CAGAATAACA AACGGTACAC ATGTGCAGAG AGTCAGGACT GTAAGATTGA TAAAACCCAG AGGAAACGGT GTCCTTTCTG CCGCTTCCAG AAATGTCTCA ACGTCGGGAT GCGACTGGAA GCTGTCCGTG CGGATAGGAT GCGAGGAGGC CGCAACAAAT TCGGCCCCAT GTATAAGCGA GACCGGGCGT TAAAGCAGCA GAAGAAAGCT CTCATCCGGG CCAGTGGCTT AAAGATGGAA GCCACACCCC CTTTGCTGAC GTCACCACAA CCTGACTACA CCTTCAGCAC AGCTTTGTCT GTGCCAGCTC CGAAAAACAC CCATCCGAAC ATCGTCACCT CTGTGGCACC CACCGATTAC GAACGCAGCC TCTACGCATC CAGCTCCCTG AGTTTATCCG TCCCCATCCC GGCCCACACT CCTCTCCCAG CACAATACCC CTATCCAAAT CTGCCCAGCC GTGCCATAAA GTCTGAGTAT CCTGACCACT ACACAAGCTC AGAGCATTAC ACCAGTGCCA GCTCCCCAGA GTCAGTGCCA GGCTACACGT ATATCGAGCA AACACGGGTG TCCAGCAGCC CGCAGGTGGT GGCACCGGGT TTAACAGTGC CGCCTTTGGT TCTGGAGTTT GTTCGCTGTG AGCAGGATGA GCTCCAGGTG CAAAGCAAAA TAAGCGCTCA CCTGGCGCAC CTGCAGCAGG AGCAGAACTC ACGGAGCACG GCAGCCAATC AGGAGCAGAG CTCCCGCCTC GCAGCCAAAC CCGAGCGGCT CAGCACATTT GGCCTCATGT GCCACATGGC CGACCAGACG CTCTTCTCTA TCGTGGAGTG GGCACGAAGC TGCATCTTCT TTAAGGAGCT TAAGGTTGGA GATCAGATGA AGTTGCTGCA TAACTGCTGG AGTGAACTTC TAGTGCTGGA CTATGTTGCC AGACAGTTGC ATCATGGGAA AGAGGACAGT GTGCTTCTCA TTACTGGACA GGAGGTGGAG CTTGCATCTC TCCTGGCCCA GGCAGGGGTC ACTTTGAGCG GGATGATCCA GAGAGGACAG GAGCTGGTGC AGCGTCTACA GGAGCTACAG CTGGACCGCA GAGAAACCGC CTGCCTCAAA TACCTCATCC TCTTTAACCC CGATGTGAAA CTGTTGGAAA ACCAGCCTTA CGTGGAGAGC GTGTACGAGC AGGTGAACGC CGCGCTGTTG GAGTACACGC TGTGCGCGTA TCCGCAATTC CCGGACAGGT TTAGCCAGAT TCTGCTGCGC CTTCCTGAGC TCAGAGCGCT GAGTTCGCAA GCCGAAGACT ACCTGTGCTA CAAACACCTG AGCGGAGAGG TGCCCTGCAA CAACCTCCTC ATAGAAATGC TGCATGCCAA GAGAACCTGC ATCTGACATA TGTAAGTATA CTTGATATCG ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT GGCTGATTAT GATCCTCTAG ATCAGATCTG CGAAGATACG GCCACGGGTG CTCTTGATCC TGTGGCTGAT TTTGGACTGT GCTGCTCGCA GCTGCTGATG AATCACATAC TTCCTCCATT TTCTTCCACT GATTGACTGT TATAATTTCC CTAATTTCCA GGTCAAGGTG CTGTGCATTG TGGTAATAGA TGTGACATGA CGTCACTTCC AAAGGACCAA TGAACATGTC TGACCAATTT CATATAATGT GAAAACGATT TTCATAGGCA GAATAAATAA CATTTAAATT AAACTGGGCA TCAGCGCAAT TCAATTGGTT TGGTAATAGC AAGGGAAAAT AGAATGAAGT GATCTCCAAA AAATAAGTAC TTTTTGACTG TAAATAAAAT TGTAAGGAGT AAAAAGTACT TTTTTTTCTA AAAAAATGTA ATTAAGTAAA AGTAAAAGTA TTGATTTTTA ATTGTACTCA AGTAAAGTAA AAATCCCCAA AAATAATACT TAAGTACAGT AATCAAGTAA AATTACTCAA GTACTTTACA CCTCTGGTTC TTGACCCCCT ACCTTCAGCA AGCCCAGCAG ATCCACTAGT TCTAGAGCGG CCGCCACCGC GGTGGAGCTC CAGCTTTTGT TCCCTTTAGT GAGGGTTAAT TGCGCGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTG
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