Customer Cases
Lentivirus vector is a gene therapy vector developed on the basis of HIV-1 ( human immunodeficiency virus type 1 ). It has the advantages of wide infection spectrum, effective infection of dividing and stationary cells, and long-term stable expression of exogenous genes. Therefore, it has become a powerful tool for introducing exogenous genes. Now the lentiviral system has been widely used in gene overexpression, RNA interference, microRNA research and in vivo animal experiments in various cell lines.
Lentivirus has the following characteristics :
Wide range of infection
Lentiviruses can effectively infect dividing and non-dividing cells and are suitable for almost all cell lines, such as neurons, hepatocytes, cardiomyocytes, tumor cells, endothelial cells, stem cells, etc.
Stable expression
Lentivirus can integrate foreign genes into the host cell genome without losing them with cell division and passage, and can achieve long-term stable expression of the target gene.
High operation safety
The lentivirus uses a self-inactivating replication-deficient virus strain to ensure safe operation.
Application of lentivirus :
Customer Case 1 :
Sun Yat sen Hospital of Sun Yat sen University customer Published in Nature，IF=64.8
Title : Tumour circular RNAs elicit anti-tumour immunity by encoding cryptic peptides
Methods:lentivirus pHBLV-CMV-Circ-MCS-EF1-zsgreen-T2A puro transfection of HEK 293T cells
Customer Case 2 :
Tianjin Medical University customer Published in Molecular Cancer，IF=41.444
Title : LINE-1 promotes tumorigenicity and exacerbates tumor progression via stimulating metabolism reprogramming in non-small cell lung cancer
Methods:H520 ( OV-L1-FGGY ) cells were infected with lentivirus carrying GPR31 / USP24 shRNA.
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