Customer case
Recombinant adenovirus（AdV）is a replication deficient adenovirus vector system widely used in fields such as gene therapy and basic life science research. The commonly used adenovirus vectors are based on human adenovirus type 5（Ad5），Its genome is a 36 kb long linear double stranded DNA.Adenoviruses can be internalized into cells through their own fibers and receptor binding on the cell surface, and then transferred from the endosome to the cytoplasm and nucleus, using the cell's transcription and translation machines to initiate virus replication and assembly. A complete viral life cycle can trigger cell death and release viral particles.
Adenovirus characteristics
Wide range of infection
Adenovirus can be used for infection in ordinary cell lines, as well as in suspension cells, primary cells, etc.
High infection efficiency
The titer of adenoviruses can reach up to1011PFU/ml，High infection efficiency, can infect suspended cells, primary cells, etc.
Large carrier capacity
The vector capacity of adenoviruses is large, up to 8kb.
Application of Adenovirus：
Customer Case 1：
Wuhan University customer Published in Nature Medicine, IF=82.9
Title：An ALOX12–12-HETE–GPR31 signaling axis is a key mediator of hepatic ischemia–reperfusion injury
Methods: Adenovirus infection of primary hepatocytes
Customer Case 2：
Shanghai Jiao Tong University Affiliated Renji Hospital customer Published in CELL, IF=66.85
Title：Fusobacterium nucleatum Promotes Chemoresistance to Colorectal Cancer by Modulating Autophagy
Methods: MRFP-GFP-LC3 adenovirus vector infects HCT116 cells and HT29 cells
Customer Case 3：
Southern Medical University customer Published in CELL, IF=66.85
Title：LECT2, a Ligand for Tie1, Plays a Crucial Role in Liver Fibrogenesis
Methods: Adenovirus is injected into mice through the tail vein (13 × Knock down Tie1, CD209a, and VEGFR2 for 108 pfu
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